Deletions extending from a single Ty1 element in Saccharomyces cerevisiae.

abstract：:S1 mapping of the termination region in the ribosomal DNA from a thermophilic fungus, Thermomyces lanuginosus, revealed three distinct termini corresponding to the mature 25S rRNA, a precursor that is 19 nucleotides longer and corresponds to the 37S precursor in yeast cells, and a putative termination site at +96 that...

journal_title：Molecular and cellular biology

authors： Walker K,Wong WM,Nazar RN

更新日期：1990-01-01 00:00:00

abstract：:SMAUG (SMG) is an RNA-binding protein that functions as a key component of a transcript degradation pathway that eliminates maternal mRNAs in the bulk cytoplasm of activated Drosophila melanogaster eggs. We previously showed that SMG destabilizes maternal Hsp83 mRNA by recruiting the CCR4-NOT deadenylase to trigger de...

journal_title：Molecular and cellular biology

authors： Semotok JL,Luo H,Cooperstock RL,Karaiskakis A,Vari HK,Smibert CA,Lipshitz HD

更新日期：2008-11-01 00:00:00

abstract：:The forkhead transcription factor FoxM1 has been reported to regulate, variously, proliferation and/or spindle formation during the G2/M transition of the cell cycle. Here we define specific functions of FoxM1 during brain development by the investigation of FoxM1 loss-of-function mutations in the context of Sonic hed...

journal_title：Molecular and cellular biology

authors： Schüller U,Zhao Q,Godinho SA,Heine VM,Medema RH,Pellman D,Rowitch DH

更新日期：2007-12-01 00:00:00

abstract：:Human myeloid leukemia cells respond to 12-O-tetradecanoylphorbol-13-acetate (TPA) and other activators of protein kinase C (PKC) with induction of monocytic differentiation. The present studies demonstrated that treatment of U-937 and HL-60 myeloid leukemia cells with TPA, phorbol-12,13-dibutyrate, or bryostatin 1 wa...

journal_title：Molecular and cellular biology

authors： Kaneki M,Kharbanda S,Pandey P,Yoshida K,Takekawa M,Liou JR,Stone R,Kufe D

更新日期：1999-01-01 00:00:00

abstract：:T-cell activation requires two different signals. The T-cell receptor's recognition of a specific antigen on antigen-presenting cells provides one, and the second signal comes from costimulatory molecules such as CD28. In contrast, T cells that are stimulated with antigen in the absence of the CD28 costimulatory signa...

journal_title：Molecular and cellular biology

authors： Lai JH,Horvath G,Subleski J,Bruder J,Ghosh P,Tan TH

更新日期：1995-08-01 00:00:00

abstract：:The SNF3 gene of Saccharomyces cerevisiae encodes a high-affinity glucose transporter that is homologous to mammalian glucose transporters. Point mutations affecting the function of the transporter were recovered from the genomes of four snf3 mutants and characterized. Two of the mutations introduced a charged amino a...

journal_title：Molecular and cellular biology

authors： Marshall-Carlson L,Celenza JL,Laurent BC,Carlson M

更新日期：1990-03-01 00:00:00

abstract：:RBM25 has been shown to associate with splicing cofactors SRm160/300 and assembled splicing complexes, but little is known about its splicing regulation. Here, we characterize the functional role of RBM25 in alternative pre-mRNA splicing. Increased RBM25 expression correlated with increased apoptosis and specifically ...

journal_title：Molecular and cellular biology

authors： Zhou A,Ou AC,Cho A,Benz EJ Jr,Huang SC

更新日期：2008-10-01 00:00:00

abstract：:The 12p13 ETV6 (TEL) gene is frequently targeted by chromosomal translocations in human malignancies, resulting in the formation of oncogenic ETV6 gene fusions. Many of the known partner genes encode protein tyrosine kinases (PTKs), generating fusion proteins that function as chimeric PTKs. ETV6-NTRK3 (EN), comprised ...

journal_title：Molecular and cellular biology

authors： Tognon CE,Mackereth CD,Somasiri AM,McIntosh LP,Sorensen PH

更新日期：2004-06-01 00:00:00

abstract：:The opposing effects on proliferation mediated by G-protein-coupled receptor isoforms differing in their COOH termini could be correlated with the abilities of the receptors to differentially activate p38, implicated in apoptotic events, or phosphatidylinositol 3-kinase (PI 3-K), which provides a source of survival si...

journal_title：Molecular and cellular biology

authors： Sellers LA,Alderton F,Carruthers AM,Schindler M,Humphrey PP

更新日期：2000-08-01 00:00:00

abstract：:Eukaryotic ribosome biogenesis requires the action of approximately 200 trans-acting factors and the incorporation of 79 ribosomal proteins (RPs). The delivery of RPs to preribosomes is a major challenge for the cell because RPs are often highly basic and contain intrinsically disordered regions prone to nonspecific i...

journal_title：Molecular and cellular biology

authors： Black JJ,Musalgaonkar S,Johnson AW

更新日期：2019-08-12 00:00:00

abstract：:Rpd3(L) and Rpd3(S) are distinct multisubunit complexes containing the Rpd3 histone deacetylase. Disruption of the GCN5 histone acetyltransferase gene shows a strong synthetic phenotype when combined with either an sds3 mutation affecting only the Rpd3(L) complex or an rco1 mutation affecting only Rpd3(S). However, th...

journal_title：Molecular and cellular biology

authors： Biswas D,Takahata S,Stillman DJ

更新日期：2008-07-01 00:00:00

abstract：:The E2F transcription factors are key downstream targets of the retinoblastoma protein (pRB) tumor suppressor. We have previously shown that E2F3 plays a critical role in mediating the mitogen-induced activation of E2F-responsive genes and contributes to both the inappropriate proliferation and the p53-dependent apopt...

journal_title：Molecular and cellular biology

authors： Ziebold U,Lee EY,Bronson RT,Lees JA

更新日期：2003-09-01 00:00:00

abstract：:Protein 4.1G is a membrane skeletal protein found in specific subcellular structures in myelinated Schwann cells and seminiferous tubules. Here, we show that in the mouse sciatic nerve, protein 4.1G colocalized at Schmidt-Lanterman incisures (SLI) and the paranodes with a member of the membrane-associated guanylate ki...

journal_title：Molecular and cellular biology

authors： Terada N,Saitoh Y,Ohno N,Komada M,Saitoh S,Peles E,Ohno S

更新日期：2012-01-01 00:00:00

abstract：:Gene targeting is a very powerful tool for studying mammalian development and physiology and for creating models of human diseases. In many instances, however, it is desirable to study different modifications of a target gene, but this is limited by the generally low frequency of homologous recombination in mammalian ...

journal_title：Molecular and cellular biology

authors： Cohen-Tannoudji M,Robine S,Choulika A,Pinto D,El Marjou F,Babinet C,Louvard D,Jaisser F

更新日期：1998-03-01 00:00:00

abstract：:U6 is the most conserved of the five small nuclear RNAs known to participate in pre-mRNA splicing. In the fission yeast Schizosaccharomyces pombe, the single-copy gene encoding this RNA is itself interrupted by an intron (T. Tani and Y. Ohshima, Nature (London) 337:87-90, 1989). Here we report analysis of the U6 genes...

journal_title：Molecular and cellular biology

authors： Reich C,Wise JA

更新日期：1990-10-01 00:00:00

abstract：:We have previously formulated a list of approximately 2,000 RNA octamers as putative exonic splicing enhancers (PESEs) based on a statistical comparison of human exonic and nonexonic sequences (X. H. Zhang and L. A. Chasin, Genes Dev. 18:1241-1250, 2004). When inserted into a poorly spliced test exon, all eight tested...

journal_title：Molecular and cellular biology

authors： Zhang XH,Kangsamaksin T,Chao MS,Banerjee JK,Chasin LA

更新日期：2005-08-01 00:00:00

abstract：:Pretreatment of 3T6 murine cells with the carcinogen UV radiation or N-acetoxy-N-acetylaminofluorene increased the number of methotrexate-resistant colonies. This carcinogen-induced enhancement was seen only at low toxicities. The enhancement was transient and was observed at its maximum when cells were subjected to m...

journal_title：Molecular and cellular biology

authors： Tlsty TD,Brown PC,Schimke RT

更新日期：1984-06-01 00:00:00

abstract：:hsa-miR-33a and hsa-miR-33b, intronic microRNAs (miRNAs) located within the sterol regulatory element-binding protein 2 and 1 genes (Srebp-2 and -1), respectively, have recently been shown to regulate lipid homeostasis in concert with their host genes. Although the functional role of miR-33a and -b has been highly inv...

journal_title：Molecular and cellular biology

authors： Goedeke L,Vales-Lara FM,Fenstermaker M,Cirera-Salinas D,Chamorro-Jorganes A,Ramírez CM,Mattison JA,de Cabo R,Suárez Y,Fernández-Hernando C

更新日期：2013-06-01 00:00:00

abstract：:In eukaryotes, crossovers in mitotic cells can have deleterious consequences and therefore must be suppressed. Mutations in BLM give rise to Bloom syndrome, a disease that is characterized by an elevated rate of crossovers and increased cancer susceptibility. However, simple eukaryotes such as Saccharomyces cerevisiae...

journal_title：Molecular and cellular biology

authors： Hu Y,Lu X,Barnes E,Yan M,Lou H,Luo G

更新日期：2005-05-01 00:00:00

abstract：:We have previously shown that Saccharomyces cerevisiae Isw2 complex slides nucleosomes to remodel chromatin in vivo. Our data suggested a model in which Isw2 complex binds the histone octamer and DNA separately to generate the force necessary for nucleosome movement. Here we find that the histone H4 "basic patch" is t...

journal_title：Molecular and cellular biology

authors： Fazzio TG,Gelbart ME,Tsukiyama T

更新日期：2005-11-01 00:00:00

abstract：:TEL1 is important in Saccharomyces cerevisiae telomere maintenance, and its kinase activity is required. Tel1p associates with telomeres in vivo, is enriched at short telomeres, and enhances the binding of telomerase components to short telomeres. However, it is unclear how the kinase activity and telomere association...

journal_title：Molecular and cellular biology

authors： Ma Y,Greider CW

更新日期：2009-09-01 00:00:00

abstract：:The full-length normal and T24 mutant human H-ras proteins and two truncated derivatives of the T24 mutant were expressed efficiently in Escherichia coli. The proteins accumulated to 1 to 5% of total cellular protein, and each was specifically recognized by anti-ras monoclonal antibodies. The two full-length proteins ...

journal_title：Molecular and cellular biology

authors： Gross M,Sweet RW,Sathe G,Yokoyama S,Fasano O,Goldfarb M,Wigler M,Rosenberg M

更新日期：1985-05-01 00:00:00

abstract：:Activation of oncogenes or inhibition of WEE1 kinase deregulates cyclin-dependent kinase (CDK) activity and leads to replication stress; however, the underlying mechanism is not understood. We now show that elevation of CDK activity by inhibition of WEE1 kinase rapidly increases initiation of replication. This leads t...

journal_title：Molecular and cellular biology

authors： Beck H,Nähse-Kumpf V,Larsen MS,O'Hanlon KA,Patzke S,Holmberg C,Mejlvang J,Groth A,Nielsen O,Syljuåsen RG,Sørensen CS

更新日期：2012-10-01 00:00:00

abstract：:A region located at kbp -3.9 to -2.6 5' to the first hematopoietic exon of the GATA-1 gene is necessary to recapitulate gene expression in both the primitive and definitive erythroid lineages. In transfection analyses, this region activated reporter gene expression from an artificial promoter in a position- and orient...

journal_title：Molecular and cellular biology

authors： Nishimura S,Takahashi S,Kuroha T,Suwabe N,Nagasawa T,Trainor C,Yamamoto M

更新日期：2000-01-01 00:00:00

abstract：:Barsoum and Varshavsky (Proc. Natl. Acad. Sci. U.S.A. 80:5330-5334, 1983) suggest that polypeptide mitogens and the mitogenic tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) stimulate gene amplification by related pathways. I demonstrated that TPA and the polypeptide mitogen fibroblast growth factor (FGF) bo...

journal_title：Molecular and cellular biology

authors： Herschman HR

更新日期：1985-05-01 00:00:00

abstract：:In Saccharomyces cerevisiae (budding yeast), commitment to cell division in late G(1) is promoted by the G(1) cyclin Cln3 and its associated cyclin-dependent kinase, Cdc28. We show here that all known aspects of the function of Cln3 in G(1) phase, including control of cell size, pheromone sensitivity, cell cycle progr...

journal_title：Molecular and cellular biology

authors： Wijnen H,Landman A,Futcher B

更新日期：2002-06-01 00:00:00

abstract：:We employed a protein gel blotting procedure to search for nuclear proteins from rat pituitary cells that bind preferentially to the 5'-flanking region of the rat prolactin gene. By gel blots of chromatin proteins from GH3 rat pituitary tumor cells with a 32P-labeled prolactin genomic clone, we detected two major bind...

journal_title：Molecular and cellular biology

authors： White BA,Preston GM,Lufkin TC,Bancroft C

更新日期：1985-11-01 00:00:00

abstract：:Inhibition of mitosis by antimitotic drugs is thought to occur by destruction of microtubules, causing cells to arrest through the action of one or more mitotic checkpoints. We have patterned experiments in the yeast Saccharomyces cerevisiae after recent studies in mammalian cells that demonstrate the effectiveness of...

journal_title：Molecular and cellular biology

authors： Wang Y,Burke DJ

更新日期：1995-12-01 00:00:00

abstract：:The recruitment of transcriptional coactivators, including histone modifying enzymes, is an important step in transcription regulation. A typical activator is thought to interact with several cofactors, presumably in a sequential manner. The common use of several cofactors raises the question of how activators achieve...

journal_title：Molecular and cellular biology

authors： Gamper AM,Roeder RG

更新日期：2008-04-01 00:00:00

abstract：:Oncogenic activation of the mitogen-activated protein (MAP) kinase cascade in murine fibroblasts initiates a senescence-like cell cycle arrest that depends on the ARF/p53 tumor suppressor pathway. To investigate whether p53 is sufficient to induce senescence, we introduced a conditional murine p53 allele (p53(val135))...

journal_title：Molecular and cellular biology

authors： Ferbeyre G,de Stanchina E,Lin AW,Querido E,McCurrach ME,Hannon GJ,Lowe SW

更新日期：2002-05-01 00:00:00