Two distinct mechanisms of chromatin interaction by the Isw2 chromatin remodeling complex in vivo.

Abstract:

:We have previously shown that Saccharomyces cerevisiae Isw2 complex slides nucleosomes to remodel chromatin in vivo. Our data suggested a model in which Isw2 complex binds the histone octamer and DNA separately to generate the force necessary for nucleosome movement. Here we find that the histone H4 "basic patch" is the only portion of any amino-terminal histone tail required for both target-specific association of Isw2 complex with chromatin and chromatin remodeling in vivo, whereas it is dispensable for basal levels of chromatin binding. Similarly, we find that nonremodeled chromatin structure and integrity of Isw2 complex are required only for target-specific association of Isw2 with chromatin. These data demonstrate fundamental differences between the target-specific and basal modes of chromatin binding by Isw2 complex in vivo and suggest that only the former involves contributions from DNA, histone H4, and sequence-specific DNA binding proteins. We propose a model for target recognition and chromatin remodeling by Isw2 complex in vivo.

journal_name

Mol Cell Biol

authors

Fazzio TG,Gelbart ME,Tsukiyama T

doi

10.1128/MCB.25.21.9165-9174.2005

subject

Has Abstract

pub_date

2005-11-01 00:00:00

pages

9165-74

issue

21

eissn

0270-7306

issn

1098-5549

pii

25/21/9165

journal_volume

25

pub_type

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