Novel splicing factor RBM25 modulates Bcl-x pre-mRNA 5' splice site selection.

abstract：:The Drosophila melanogaster Myb-MuvB/dREAM complex (MMB/dREAM) participates in both the activation and repression of developmentally regulated genes and origins of DNA replication. Mutants in MMB subunits exhibit diverse phenotypes, including lethality, eye defects, reduced fecundity, and sterility. Here, we used P-el...

journal_title：Molecular and cellular biology

authors： Lewis PW,Sahoo D,Geng C,Bell M,Lipsick JS,Botchan MR

更新日期：2012-08-01 00:00:00

abstract：:The biosynthesis, phosphorylation, and degradation of the epidermal growth factor (EGF) receptor were examined in normal human fibroblasts. The receptor was initially synthesized as an Mr = 160,000 immature form which matured to an Mr = 170,000 form in a monensin-sensitive manner. Tunicamycin treatment led to the accu...

journal_title：Molecular and cellular biology

authors： Decker SJ

更新日期：1984-09-01 00:00:00

abstract：:The treatment of cells with histone deacetylase inhibitors (HDACi) was reported to reveal the acetylation of STAT1 at lysine 410 and lysine 413 (O. H. Krämer et al., Genes Dev. 20:473-485, 2006). STAT1 acetylation was proposed to regulate apoptosis by facilitating binding to NF-κB and to control immune responses by su...

journal_title：Molecular and cellular biology

authors： Antunes F,Marg A,Vinkemeier U

更新日期：2011-07-01 00:00:00

abstract：:An enzyme that hydrolyzes one PPi bond of the cap structure of mRNA, yielding m7GDP and 5'-p RNA was purified from Saccharomyces cerevisiae to a stage suitable for characterization. The specificity of the enzyme was studied, using both yeast mRNA and synthetic RNAs labeled in the cap structure. A synthetic capped RNA ...

journal_title：Molecular and cellular biology

authors： Stevens A

更新日期：1988-05-01 00:00:00

abstract：:SR proteins are essential for pre-mRNA splicing in vitro, act early in the splicing pathway, and can influence alternative splice site choice. Here we describe the isolation of both dominant and loss-of-function alleles of B52, the gene for a Drosophila SR protein. The allele B52ED was identified as a dominant second-...

journal_title：Molecular and cellular biology

authors： Peng X,Mount SM

更新日期：1995-11-01 00:00:00

abstract：:Inactivating mutations within the von Hippel-Lindau (VHL) tumor suppressor gene predispose patients to develop a variety of highly vascularized tumors. pVHL targets alpha subunits of the heterodimeric transcription factor hypoxia-inducible factor (HIF), a critical regulator of energy metabolism, angiogenesis, hematopo...

journal_title：Molecular and cellular biology

authors： Mack FA,Patel JH,Biju MP,Haase VH,Simon MC

更新日期：2005-06-01 00:00:00

abstract：:Hybridization to synthetic oligonucleotides representing conserved regions in the promoter and first intron of several vertebrate beta-actin genes was used to discriminate between what appears to be a single functional beta-actin gene and numerous pseudogenes in the mouse genome. Sequences derived from the 5' end of t...

journal_title：Molecular and cellular biology

authors： Elder PK,French CL,Subramaniam M,Schmidt LJ,Getz MJ

更新日期：1988-01-01 00:00:00

abstract：:The mRNA for transforming growth factor beta 3 (TGF-beta 3) includes a long (1.1-kb) 5' noncoding region which exerts a potent inhibitory effect on translational efficiency. We now report that many human breast cancer cell lines (T47-D, SK-BR-3, ZR-75-1, and BT-474) express two mRNA species for TGF-beta 3: the 3.5-kb ...

journal_title：Molecular and cellular biology

authors： Arrick BA,Grendell RL,Griffin LA

更新日期：1994-01-01 00:00:00

abstract：:Human insulin-like growth factor II (IGF-II) mRNAs are subject to site-specific endonucleolytic cleavage in the 3' untranslated region, leading to an unstable 5' cleavage product containing the IGF-II coding region and a very stable 3' cleavage product of 1.8 kb. This endonucleolytic cleavage is most probably the firs...

journal_title：Molecular and cellular biology

authors： Scheper W,Meinsma D,Holthuizen PE,Sussenbach JS

更新日期：1995-01-01 00:00:00

abstract：:Selective and accurate transcription of purified genes by RNA polymerase II requires multiple factors. The factor designated TFIID was purified extensively from HeLa cell nuclear extracts by using a simple and novel complementation assay. Thus, TFIID was preferentially inactivated by mild heat treatment of a nuclear e...

journal_title：Molecular and cellular biology

authors： Nakajima N,Horikoshi M,Roeder RG

更新日期：1988-10-01 00:00:00

abstract：:The pluripotent factor Oct4 is a key transcription factor that maintains embryonic stem (ES) cell self-renewal and is down-regulated upon the differentiation of ES cells and silenced in somatic cells. A combination of cis elements, transcription factors, and epigenetic modifications, such as DNA methylation, are invol...

journal_title：Molecular and cellular biology

authors： Gu P,Le Menuet D,Chung AC,Cooney AJ

更新日期：2006-12-01 00:00:00

abstract：:Numb proteins are evolutionarily conserved signaling molecules that make the daughter cells different after asymmetric divisions by segregating to only one daughter. They contain distinct binding motifs for alpha-adaptin (alpha-Ada) and proteins with Eps15 homology (EH) domains, which regulate endocytosis, and for E3 ...

journal_title：Molecular and cellular biology

authors： Tang H,Rompani SB,Atkins JB,Zhou Y,Osterwalder T,Zhong W

更新日期：2005-04-01 00:00:00

abstract：:Plasmids containing the hormone regulatory element of mouse mammary tumor virus linked to the thymidine kinase promoter of herpes simplex virus and the reporter gene chloramphenicol acetyltransferase of Escherichia coli respond to glucocorticoids and progestins when transfected into appropriate cells. In the human mam...

journal_title：Molecular and cellular biology

authors： Piña B,Haché RJ,Arnemann J,Chalepakis G,Slater EP,Beato M

更新日期：1990-02-01 00:00:00

abstract：:Gonadotropin-releasing hormone (GnRH) interacts with a G protein-coupled receptor and increases the transcription of the glycoprotein hormone alpha-subunit gene. We have explored the possibility that mitogen-activated protein kinase (MAPK) plays a role in mediating GnRH effects on transcription. Activation of the MAPK...

journal_title：Molecular and cellular biology

authors： Roberson MS,Misra-Press A,Laurance ME,Stork PJ,Maurer RA

更新日期：1995-07-01 00:00:00

abstract：:Phosphorylation on tyrosine residues mediated by pp60src appears to be a primary biochemical event leading to the establishment of the transformed phenotype in Rous sarcoma virus (RSV)-infected cells. To identify the cellular proteins that undergo tyrosine phosphorylation during transformation, a 32P-labeled RSV-trans...

journal_title：Molecular and cellular biology

authors： Martinez R,Nakamura KD,Weber MJ

更新日期：1982-06-01 00:00:00

abstract：:When exposed to 5-azacytidine, marrow stromal cells from active long-term marrow cultures and cell lines derived from simian virus 40-transformed stromal cells rapidly upregulated c-abl and interleukin-6 transcripts while downregulating the expression of collagen I, a major matrix protein. Similar effects occurred wit...

journal_title：Molecular and cellular biology

authors： Andrews DF 3rd,Nemunaitis J,Tompkins C,Singer JW

更新日期：1989-06-01 00:00:00

abstract：:Nearly 40% of cases of acute myelogenous leukemia (AML) of the M2 subtype are due to a chromosomal translocation that combines a sequence-specific DNA binding protein, AML1, with a potent transcriptional repressor, ETO. ETO interacts with nuclear receptor corepressors SMRT and N-CoR, which recruit histone deacetylase ...

journal_title：Molecular and cellular biology

authors： Zhang J,Hug BA,Huang EY,Chen CW,Gelmetti V,Maccarana M,Minucci S,Pelicci PG,Lazar MA

更新日期：2001-01-01 00:00:00

abstract：:Initiation of T-lymphocyte-mediated immune responses involves two cellular processes: entry into the cell cycle (G(0)-->G(1)) for clonal proliferation and coordinated changes in surface and secreted molecules that mediate effector functions. However, a point during G(0)-->G(1) beyond which T cells are committed to ent...

journal_title：Molecular and cellular biology

authors： Lea NC,Orr SJ,Stoeber K,Williams GH,Lam EW,Ibrahim MA,Mufti GJ,Thomas NS

更新日期：2003-04-01 00:00:00

abstract：:The LINE-1, or L1, family of interspersed repeated DNA constitutes roughly 10% of the mammalian genome. Its abundance is due to duplicative transposition via an RNA intermediate, L1-encoded proteins, and reverse transcription. Although, in principle, transposition may occur in any cell type, expression and transpositi...

journal_title：Molecular and cellular biology

authors： Branciforte D,Martin SL

更新日期：1994-04-01 00:00:00

abstract：:Low oxygen gradients (hypoxia and anoxia) are important determinants of pathological conditions under which the tissue blood supply is deficient or defective, such as in solid tumors. We have been investigating the relationship between the activation of hypoxia-inducible factor 1 (HIF-1), the primary transcriptional r...

journal_title：Molecular and cellular biology

authors： Laderoute KR,Amin K,Calaoagan JM,Knapp M,Le T,Orduna J,Foretz M,Viollet B

更新日期：2006-07-01 00:00:00

abstract：:The genes of the trithorax group (trxG) in Drosophila melanogaster are required to maintain the pattern of homeotic gene expression that is established early in embryogenesis by the transient expression of the segmentation genes. The precise role of each of the diverse trxG members and the functional relationships amo...

journal_title：Molecular and cellular biology

authors： Crosby MA,Miller C,Alon T,Watson KL,Verrijzer CP,Goldman-Levi R,Zak NB

更新日期：1999-02-01 00:00:00

abstract：:The ESC-E(Z) complex of Drosophila melanogaster Polycomb group (PcG) repressors is a histone H3 methyltransferase (HMTase). This complex silences fly Hox genes, and related HMTases control germ line development in worms, flowering in plants, and X inactivation in mammals. The fly complex contains a catalytic SET domai...

journal_title：Molecular and cellular biology

authors： Ketel CS,Andersen EF,Vargas ML,Suh J,Strome S,Simon JA

更新日期：2005-08-01 00:00:00

abstract：:Transcriptional insulators are cis regulatory elements that organize chromatin into independently regulated domains. At the imprinted murine Igf2/H19 locus, the H19-ICR insulator prevents the activation of the Igf2 promoter on the maternal allele by enhancers that activate H19 on the same chromosome. Given the well-de...

journal_title：Molecular and cellular biology

authors： Singh V,Srivastava M

更新日期：2008-06-01 00:00:00

abstract：:p27(Kip1) is a member of the Cip-Kip family of cyclin-dependent kinase (Cdk) inhibitors that binds to cyclin-Cdk complexes and inhibits their catalytic activity in response to antiproliferative stimuli. p27(Kip1) is regulated by several posttranscriptional mechanisms, including subcellular localization. We have identi...

journal_title：Molecular and cellular biology

authors： Smitherman M,Lee K,Swanger J,Kapur R,Clurman BE

更新日期：2000-08-01 00:00:00

abstract：:Tetraspanins are clustered in specific microdomains (named tetraspanin-enriched microdomains, or TERM) in the plasma membrane and regulate the functions of associated transmembrane receptors, including integrins and receptor tyrosine kinases. We have identified syntenin-1, a PDZ domain-containing protein, as a new com...

journal_title：Molecular and cellular biology

authors： Latysheva N,Muratov G,Rajesh S,Padgett M,Hotchin NA,Overduin M,Berditchevski F

更新日期：2006-10-01 00:00:00

abstract：:The mTOR signaling complex integrates signals from growth factors and nutrient availability to control cell growth and proliferation, in part through effects on the protein-synthetic machinery. Protein synthesis rates fluctuate throughout the cell cycle but diminish significantly during the G(2)/M transition. The fate...

journal_title：Molecular and cellular biology

authors： Ramírez-Valle F,Badura ML,Braunstein S,Narasimhan M,Schneider RJ

更新日期：2010-07-01 00:00:00

abstract：:We analyzed epsilon-globin transcription in erythroid cells and in erythroid extracts to determine the requirements for enhancer-dependent expression of this gene. Mutations that abolished GATA-1 binding at a single position in the promoter prevented interaction with enhancers, whereas elimination of a second more dis...

journal_title：Molecular and cellular biology

authors： Gong Q,Dean A

更新日期：1993-02-01 00:00:00

abstract：:Zinc finger genes encode proteins that act as transcription factors. The myeloid zinc finger 1 (MZF1) gene encodes a zinc finger protein with two DNA-binding domains that recognize two distinct consensus sequences, is preferentially expressed in hematopoietic cells, and may be involved in the transcriptional regulatio...

journal_title：Molecular and cellular biology

authors： Perrotti D,Melotti P,Skorski T,Casella I,Peschle C,Calabretta B

更新日期：1995-11-01 00:00:00

abstract：:After flagellar detachment in Chlamydomonas reinhardi, there is a rapid synthesis and accumulation of mRNAs for tubulin and other flagellar proteins. Maximum levels of these mRNAs (flagellar RNAs) are reached within 1 h after deflagellation, after which they are rapidly degraded to their predeflagellation levels. The ...

journal_title：Molecular and cellular biology

authors： Baker EJ,Keller LR,Schloss JA,Rosenbaum JL

更新日期：1986-01-01 00:00:00

abstract：:An empirical equation has been developed that can be used to calculate genetic map distances from tetrad data with good accuracy for distances of up to at least 120 centimorgans. ...

journal_title：Molecular and cellular biology

authors： Ma C,Mortimer RK

更新日期：1983-10-01 00:00:00