Abstract:
:The Ste12p transcription factor controls the expression of Ty1 transposable element insertion mutations and genes whose products are required for mating in Saccharomyces cerevisiae. The binding site for Ste12p is a consensus DNA sequence known as a pheromone response element (PRE). Upstream activating sequences (UASs) derived from known Ste12p-dependent genes have previously been characterized to require either multiple PREs or a single PRE coupled to a binding site for a second protein. The Ste12p-dependent UAS from Ty1, called a sterile response element (SRE), is of the second type and is comprised of a PRE and an adjacent TEA (TEF-1, Tec1, and AbaA motif) DNA consensus sequence (TCS). In this report, we show by UV cross-linking analysis that two proteins, Ste12p and a protein with an apparent size of 72 kDa, directly contact the Ty1 SRE. Other experiments show that Tec1p is required for formation of the Ty1 SRE protein-DNA complex and is physically present in the complex. These results establish a direct role for Tec1p in the Ty1 SRE and yet another set of combinatorial interactions that achieve a qualitatively distinct mode of transcriptional regulation with Ste12p.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Baur M,Esch RK,Errede Bdoi
10.1128/mcb.17.8.4330subject
Has Abstractpub_date
1997-08-01 00:00:00pages
4330-7issue
8eissn
0270-7306issn
1098-5549journal_volume
17pub_type
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.18.10.5828
更新日期:1998-10-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.10.1.333
更新日期:1990-01-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1988-07-01 00:00:00
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更新日期:1999-03-01 00:00:00
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更新日期:2009-03-01 00:00:00
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更新日期:1999-12-01 00:00:00