Abstract:
:We have combined in vitro DNA replication reactions and immunological techniques to analyze biochemical interactions between simian virus (SV40) large T antigen and components of the cellular replication apparatus. First, in vitro SV40 DNA replication was characterized with specific origin mutants. Next, monoclonal antibodies were used to demonstrate that a specific domain of T antigen formed a complex with cellular DNA polymerase alpha. Several antibodies were identified that coprecipitated T antigen and DNA polymerase alpha, while others were found to selectively prevent this interaction and concomitantly inhibit DNA replication. DNA polymerase alpha also bound efficiently to a T-antigen affinity column, confirming the immunoprecipitation results and providing a useful method for purification of the complete protein complex. Taken together, these results suggest that the T-antigen-polymerase association may be a key step in the initiation of SV40 DNA replication.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Smale ST,Tjian Rdoi
10.1128/mcb.6.11.4077subject
Has Abstractpub_date
1986-11-01 00:00:00pages
4077-87issue
11eissn
0270-7306issn
1098-5549journal_volume
6pub_type
杂志文章abstract::During mammalian dosage compensation, one of two X-chromosomes in female cells is inactivated. The choice of which X is silenced can be imprinted or stochastic. Although genetic loci influencing the choice decision have been identified, the primary marks for imprinting and random selection remain undefined. Here, we e...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.8.11.4787
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pub_type: 杂志文章
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.7.4.1563
更新日期:1987-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1988-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/mcb.10.9.4737
更新日期:1990-09-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.9.3.959
更新日期:1989-03-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.11.3.1500
更新日期:1991-03-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.11.5.2832
更新日期:1991-05-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:1991-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/mcb.16.1.390
更新日期:1996-01-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:2006-06-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:1984-10-01 00:00:00
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更新日期:2002-01-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2001-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1993-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1985-02-01 00:00:00