Abstract:
:The binding of the U1 small nuclear ribonucleoprotein (snRNP)-specific proteins C, A, and 70K to U1 small nuclear RNA (snRNA) was analyzed. Assembly of U1 snRNAs from bean and soybean and a set of mutant Xenopus U1 snRNAs into U1 snRNPs in Xenopus egg extracts was studied. The ability to bind proteins was analyzed by immunoprecipitation with monospecific antibodies and by a protein-sequestering assay. The only sequence essential for binding of the U1-specific proteins was the conserved loop sequence in the 5' hairpin of U1. Further analysis suggested that protein C binds directly to the loop and that the assembly of proteins A and 70K into the RNP requires mainly protein-protein interactions. Protein C apparently recognizes a specific RNA sequence rather than a secondary structural element in the RNA.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Hamm J,van Santen VL,Spritz RA,Mattaj IWdoi
10.1128/mcb.8.11.4787subject
Has Abstractpub_date
1988-11-01 00:00:00pages
4787-91issue
11eissn
0270-7306issn
1098-5549journal_volume
8pub_type
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journal_title:Molecular and cellular biology
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journal_title:Molecular and cellular biology
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doi:10.1128/mcb.15.9.4727
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