Recombinational instability of a chimeric plasmid in Saccharomyces cerevisiae.

Abstract:

:Wild-type strains of Saccharomyces cerevisiae exhibit mitotic recombination between the chimeric plasmid TLC-1 and the endogenous 2mu circle that involves sequence homologies between the two plasmids that are not acted on by the 2mu circle site-specific recombination system. This generalized recombination can be detected because it separates the LEU2 and CAN1 markers of TLC-1 from each other through the formation of a plasmid containing only the S. cerevisiae LEU2 region and the 2mu circle. This derivative plasmid is maintained more stably during vegetative growth than TLC-1, and strains which carry it frequently lose the endogenous 2mu circle. Therefore, TLC-1 can provide a convenient selection for [cir0] cells. Formation of this new plasmid is greatly reduced, but not eliminated, in strains containing the rad52-1 mutation. This indicates that generalized mitotic recombination between plasmid sequences utilizes functions required for chromosomal recombination in S. cerevisiae.

journal_name

Mol Cell Biol

authors

Whiteway MS,Ahmed A

doi

10.1128/mcb.4.1.195

subject

Has Abstract

pub_date

1984-01-01 00:00:00

pages

195-8

issue

1

eissn

0270-7306

issn

1098-5549

journal_volume

4

pub_type

杂志文章
  • Activation of transcription by PU.1 requires both acidic and glutamine domains.

    abstract::The B-lymphocyte- and macrophage-specific transcription factor PU.1 is a member of the ets family of proteins. To understand how PU.1 functions as a transcription factor, we initiated a series of experiments to define its activation domain. Using deletion analysis, we showed that the activation domain of PU.1 is locat...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.16.1.390

    authors: Klemsz MJ,Maki RA

    更新日期:1996-01-01 00:00:00

  • Formation of vesicular stomatitis virus nucleocapsid from cytoskeletal framework-bound N protein: possible model for structure assembly.

    abstract::The pathway of vesicular stomatitis virus N protein from synthesis to assembly into capsids was studied by use of detergent extraction of infected HeLa cells together with protein cross-linking. One half of the newly synthesized N protein was extracted with the soluble cell proteins and, when cross-linked, never forme...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.4.10.2231

    authors: Chatterjee PK,Cervera MM,Penman S

    更新日期:1984-10-01 00:00:00

  • Heparin is required for cell-free binding of basic fibroblast growth factor to a soluble receptor and for mitogenesis in whole cells.

    abstract::Heparin is required for the binding of basic fibroblast growth factor (bFGF) to high-affinity receptors on cells deficient in cell surface heparan sulfate proteoglycan. So that this heparin requirement could be evaluated in the absence of other cell surface molecules, we designed a simple assay based on a genetically ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.12.1.240

    authors: Ornitz DM,Yayon A,Flanagan JG,Svahn CM,Levi E,Leder P

    更新日期:1992-01-01 00:00:00

  • Vaccinia-related kinase 2 mediates accumulation of polyglutamine aggregates via negative regulation of the chaperonin TRiC.

    abstract::Misfolding of proteins containing abnormal expansions of polyglutamine (polyQ) repeats is associated with cytotoxicity in several neurodegenerative disorders, including Huntington's disease. Recently, the eukaryotic chaperonin TRiC hetero-oligomeric complex has been shown to play an important role in protecting cells ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00756-13

    authors: Kim S,Park DY,Lee D,Kim W,Jeong YH,Lee J,Chung SK,Ha H,Choi BH,Kim KT

    更新日期:2014-02-01 00:00:00

  • Structural and functional conservation of the NuA4 histone acetyltransferase complex from yeast to humans.

    abstract::The NuA4 histone acetyltransferase (HAT) multisubunit complex is responsible for acetylation of histone H4 and H2A N-terminal tails in yeast. Its catalytic component, Esa1, is essential for cell cycle progression, gene-specific regulation and has been implicated in DNA repair. Almost all NuA4 subunits have clear homol...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.24.5.1884-1896.2004

    authors: Doyon Y,Selleck W,Lane WS,Tan S,Côté J

    更新日期:2004-03-01 00:00:00

  • Posttranscriptional regulation of chicken ccn2 gene expression by nucleophosmin/B23 during chondrocyte differentiation.

    abstract::CCN2/CTGF is a multifunctional factor that plays a crucial role in the growth and differentiation of chondrocytes. The chicken ccn2 gene is regulated not only at the transcriptional level but also by the interaction between a posttranscriptional element in the 3' untranslated region (3'-UTR) and a cofactor. In the pre...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00495-08

    authors: Mukudai Y,Kubota S,Kawaki H,Kondo S,Eguchi T,Sumiyoshi K,Ohgawara T,Shimo T,Takigawa M

    更新日期:2008-10-01 00:00:00

  • Endogenous fluctuations of DNA topology in the chloroplast of Chlamydomonas reinhardtii.

    abstract::DNA supercoiling in the chloroplast of the unicellular green alga Chlamydomonas reinhardtii was found to change with a diurnal rhythm in cells growing in alternating 12-h dark-12-h light periods. Highest and lowest DNA superhelicities occurred at the beginning and towards the end of the 12-h light periods, respectivel...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.18.12.7235

    authors: Salvador ML,Klein U,Bogorad L

    更新日期:1998-12-01 00:00:00

  • Requirement of Phosphoinositides Containing Stearic Acid To Control Cell Polarity.

    abstract::Phosphoinositides (PIPs) are present in very small amounts but are essential for cell signaling, morphogenesis, and polarity. By mass spectrometry, we demonstrated that some PIPs with stearic acyl chains were strongly disturbed in a psi1Δ Saccharomyces cerevisiae yeast strain deficient in the specific incorporation of...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00843-15

    authors: Doignon F,Laquel P,Testet E,Tuphile K,Fouillen L,Bessoule JJ

    更新日期:2016-02-16 00:00:00

  • Genetic and biochemical distinction among Chinese hamster cell emtA, emtB, and emtC mutants.

    abstract::Genetic and biochemical experiments have enabled us to more clearly distinguish three genetic loci, emtA, emtB, and emtC, all of which can be altered to give rise to resistance to the protein synthesis inhibitor, emetine, in cultured Chinese hamster cells. Genetic experiments have demonstrated that, unlike the emtB lo...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.3.3.429

    authors: Chang S,Wasmuth JJ

    更新日期:1983-03-01 00:00:00

  • Regulation of in vitro and in vivo transcription of early-region IV of adenovirus type 5 by multiple cis-acting elements.

    abstract::A series of deletion mutants spanning the promoter of the adenovirus early-region IV (EIV) gene were tested for transcriptional activity, using both in vitro and in vivo assays. Four distinct domains had additive effects on efficient transcription from the EIV promoter in HeLa whole-cell extracts. The first resided 20...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.7.7.2578

    authors: Hanaka S,Nishigaki T,Sharp PA,Handa H

    更新日期:1987-07-01 00:00:00

  • Regulation of the human cardiac/slow-twitch troponin C gene by multiple, cooperative, cell-type-specific, and MyoD-responsive elements.

    abstract::The cardiac troponin C (cTnC) gene produces identical transcripts in slow-twitch skeletal muscle and in heart muscle (R. Gahlmann, R. Wade, P. Gunning, and L. Kedes, J. Mol. Biol. 201:379-391, 1988). A separate gene encodes the fast-twitch skeletal muscle troponin C and is not expressed in heart muscle. We have used t...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.13.11.6752

    authors: Christensen TH,Prentice H,Gahlmann R,Kedes L

    更新日期:1993-11-01 00:00:00

  • Ribosome stalling is responsible for arginine-specific translational attenuation in Neurospora crassa.

    abstract::The Neurospora crassa arg-2 upstream open reading frame (uORF) plays a role in negative arginine-specific translational regulation. Primer extension inhibition analyses of arg-2 uORF-containing RNA translated in a cell-free system in which arginine-specific regulation was retained revealed "toeprints" corresponding to...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.17.9.4904

    authors: Wang Z,Sachs MS

    更新日期:1997-09-01 00:00:00

  • Cross talk between beta-adrenergic and bradykinin B(2) receptors results in cooperative regulation of cyclic AMP accumulation and mitogen-activated protein kinase activity.

    abstract::Costimulation of G protein-coupled receptors (GPCRs) may result in cross talk interactions between their downstream signaling pathways. Stimulation of GPCRs may also lead to cross talk regulation of receptor tyrosine kinase signaling and thereby to activation of mitogen-activated protein kinase (MAPK). In COS-7 cells,...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.21.24.8452-8460.2001

    authors: Hanke S,Nürnberg B,Groll DH,Liebmann C

    更新日期:2001-12-01 00:00:00

  • Analysis of Small Critical Regions of Swi1 Conferring Prion Formation, Maintenance, and Transmission.

    abstract::Saccharomyces cerevisiae contains several prion elements, which are epigenetically transmitted as self-perpetuating protein conformations. One such prion is [SWI + ], whose protein determinant is Swi1, a subunit of the SWI/SNF chromatin-remodeling complex. We previously rep...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.00206-17

    authors: Valtierra S,Du Z,Li L

    更新日期:2017-09-26 00:00:00

  • ARS2 is a conserved eukaryotic gene essential for early mammalian development.

    abstract::Determining the functions of novel genes implicated in cell survival is directly relevant to our understanding of mammalian development and carcinogenesis. ARS2 is an evolutionarily conserved gene that confers arsenite resistance on arsenite-sensitive Chinese hamster ovary cells. Little is known regarding the function...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.01565-07

    authors: Wilson MD,Wang D,Wagner R,Breyssens H,Gertsenstein M,Lobe C,Lu X,Nagy A,Burke RD,Koop BF,Howard PL

    更新日期:2008-03-01 00:00:00

  • Stabilization of a specific nuclear mRNA precursor by thyroid hormone.

    abstract::The regulation of a thyroid hormone-responsive gene in rats, designated spot 14, was explored. The expression of this gene in liver is rapidly (less than 10 min) and markedly (greater than 10-fold) altered by the administration of 3,5,3'-triiodo-L-thyronine (T3) to hypothyroid rats (P. Narayan, C. W. Liaw, and H. C. T...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.5.10.2642

    authors: Narayan P,Towle HC

    更新日期:1985-10-01 00:00:00

  • Direct interaction with Rab11a targets the epithelial Ca2+ channels TRPV5 and TRPV6 to the plasma membrane.

    abstract::TRPV5 and TRPV6 are the most Ca2+-selective members of the transient receptor potential (TRP) family of cation channels and play a pivotal role in the maintenance of Ca2+ balance in the body. However, little is known about the mechanisms controlling the plasma membrane abundance of these channels to regulate epithelia...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.26.1.303-312.2006

    authors: van de Graaf SF,Chang Q,Mensenkamp AR,Hoenderop JG,Bindels RJ

    更新日期:2006-01-01 00:00:00

  • Kinetics and regulation of the tyrosine phosphorylation of epidermal growth factor receptor in intact A431 cells.

    abstract::We have previously reported that antibodies to phosphotyrosine recognize the phosphorylated forms of platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) receptors (Zippel et al., Biochim. Biophys. Acta 881:54-61, 1986, and Sturani et al., Biochem. Biophys. Res. Commun. 137:343-350, 1986). In this r...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.8.3.1345

    authors: Sturani E,Zippel R,Toschi L,Morello L,Comoglio PM,Alberghina L

    更新日期:1988-03-01 00:00:00

  • A transcriptional insulator element, the su(Hw) binding site, protects a chromosomal DNA replication origin from position effects.

    abstract::Eukaryotic chromosomes are organized into domains of activity for both transcription and DNA replication. Transcriptional "border," or "insulator," elements have been implicated in mediating the organization of transcriptional domains. However, the DNA sequence elements which might demarcate domains of DNA replication...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.17.4.2202

    authors: Lu L,Tower J

    更新日期:1997-04-01 00:00:00

  • Stabilization of tubulin mRNA by inhibition of protein synthesis in sea urchin embryos.

    abstract::An increased level of unpolymerized tubulin caused by depolymerization of microtubules in sea urchin larvae resulted in a rapid loss of tubulin mRNA, which was prevented by nearly complete inhibition of protein synthesis. Results of an RNA run-on assay indicated that inhibition of protein synthesis does not alter tubu...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.8.8.3518

    authors: Gong ZY,Brandhorst BP

    更新日期:1988-08-01 00:00:00

  • Bioluminescence imaging captures the expression and dynamics of endogenous p21 promoter activity in living mice and intact cells.

    abstract::To interrogate endogenous p21(WAF1/CIP1) (p21) promoter activity under basal conditions and in response to various forms of stress, knock-in imaging reporter mice in which expression of firefly luciferase (FLuc) was placed under the control of the endogenous p21 promoter within the Cdkn1a gene locus were generated. Bi...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.05243-11

    authors: Tinkum KL,Marpegan L,White LS,Sun J,Herzog ED,Piwnica-Worms D,Piwnica-Worms H

    更新日期:2011-09-01 00:00:00

  • Modular recognition of 5-base-pair DNA sequence motifs by human heat shock transcription factor.

    abstract::We investigated the recognition of the conserved 5-bp repeated motif NGAAN, which occurs in heat shock gene promoters of Drosophila melanogaster and other eukaryotic organisms, by human heat shock transcription factor (HSF). Extended heat shock element mutants of the human HSP70 gene promoter, containing additional NG...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.11.7.3504

    authors: Cunniff NF,Wagner J,Morgan WD

    更新日期:1991-07-01 00:00:00

  • Interactions of Pex7p and Pex18p/Pex21p with the peroxisomal docking machinery: implications for the first steps in PTS2 protein import.

    abstract::Peroxisomal PTS2-dependent matrix protein import starts with the recognition of the PTS2 targeting signal by the import receptor Pex7p. Subsequently, the formed Pex7p/cargo complex is transported from the cytosol to the peroxisomal docking complex, consisting of Pex13p and Pex14p. In Saccharomyces cerevisiae, the latt...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.22.17.6056-6069.2002

    authors: Stein K,Schell-Steven A,Erdmann R,Rottensteiner H

    更新日期:2002-09-01 00:00:00

  • Native polycystin 2 functions as a plasma membrane Ca2+-permeable cation channel in renal epithelia.

    abstract::Mutations in polycystin 2 (PC2), a Ca(2+)-permeable cation channel, cause autosomal dominant polycystic kidney disease. Whether PC2 functions in the endoplasmic reticulum (ER) or in the plasma membrane has been controversial. Here we generated and characterized a polyclonal antibody against PC2, determined the subcell...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.23.7.2600-2607.2003

    authors: Luo Y,Vassilev PM,Li X,Kawanabe Y,Zhou J

    更新日期:2003-04-01 00:00:00

  • Nuclear envelope localization of an adenovirus tumor antigen maintains the integrity of cellular DNA.

    abstract::The adenovirus early-region 1B 19,000-molecular-weight tumor antigen is required for oncogenic transformation of cells by adenovirus. We have demonstrated that this tumor antigen is located in the nuclear envelope of infected and transformed cells and that a fraction of the protein within the nuclear envelope is assoc...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.4.12.2865

    authors: White E,Blose SH,Stillman BW

    更新日期:1984-12-01 00:00:00

  • Roles of hnRNP A1, SR proteins, and p68 helicase in c-H-ras alternative splicing regulation.

    abstract::Human ras genes play central roles in coupling extracellular signals with complex intracellular networks controlling proliferation, differentiation, and apoptosis, among others processes. c-H-ras pre-mRNA can be alternatively processed into two mRNAs due to the inclusion or exclusion of the alternative exon IDX; this ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.23.8.2927-2941.2003

    authors: Guil S,Gattoni R,Carrascal M,Abián J,Stévenin J,Bach-Elias M

    更新日期:2003-04-01 00:00:00

  • Capn5 is expressed in a subset of T cells and is dispensable for development.

    abstract::The Capn5 gene was inactivated by homologous recombination in ES cells that subsequently colonized the germ line of mice. The targeted mutation integrated a lacZ expression cassette into the Capn5 gene, allowing the expression of Capn5 mRNA to be examined in detail in heterozygous animals. Expression was observed in e...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.24.4.1649-1654.2004

    authors: Franz T,Winckler L,Boehm T,Dear TN

    更新日期:2004-02-01 00:00:00

  • The transcription factor RFX3 directs nodal cilium development and left-right asymmetry specification.

    abstract::There are five members of the RFX family of transcription factors in mammals. While RFX5 plays a well-defined role in the immune system, the functions of RFX1 to RFX4 remain largely unknown. We have generated mice with a deletion of the Rfx3 gene. RFX3-deficient mice exhibit frequent left-right (LR) asymmetry defects ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.24.10.4417-4427.2004

    authors: Bonnafe E,Touka M,AitLounis A,Baas D,Barras E,Ucla C,Moreau A,Flamant F,Dubruille R,Couble P,Collignon J,Durand B,Reith W

    更新日期:2004-05-01 00:00:00

  • Analysis of ATF3, a transcription factor induced by physiological stresses and modulated by gadd153/Chop10.

    abstract::We demonstrate that ATF3, a member of the ATF/CREB family of transcription factors, is induced in a variety of stressed tissues: mechanically injured liver, toxin-injured liver, blood-deprived heart, and postseizure brain. We also demonstrate that an ATF3-interacting protein, gadd153/Chop10, forms a nonfunctional hete...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/mcb.16.3.1157

    authors: Chen BP,Wolfgang CD,Hai T

    更新日期:1996-03-01 00:00:00

  • Scaffolding protein Gab2 mediates differentiation signaling downstream of Fms receptor tyrosine kinase.

    abstract::Fms is the receptor for macrophage colony-stimulating factor (M-CSF) and contains intrinsic tyrosine kinase activity. Expression of exogenous Fms in a murine myeloid progenitor cell line, FDC-P1 (FD-Fms), results in M-CSF-dependent growth and macrophage differentiation. Previously, we described a 100-kDa protein that ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章

    doi:10.1128/MCB.21.9.3047-3056.2001

    authors: Liu Y,Jenkins B,Shin JL,Rohrschneider LR

    更新日期:2001-05-01 00:00:00