Abstract:
:DNA in the polyploid macronucleus of the ciliated protozoan Tetrahymena thermophila contains the modified base N6-methyladenine. We identified two GATC sites which are methylated in most or all of the 45 copies of the macronuclear genome. One site is 2 kilobases 5' to the histone H4-I gene, and the other is 5 kilobases 3' to the 73-kilodalton heat shock protein gene. These sites are de novo methylated between 10 and 16 h after initiation of conjugation, during macronuclear anlage development. The methylation states of these two GATC sites and four other unmethylated GATC sites do not change in the DNA of cells cultured under conditions which change the activity of the genes, including logarithmic growth, starvation, and heat shock.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Harrison GS,Findly RC,Karrer KMdoi
10.1128/mcb.6.7.2364subject
Has Abstractpub_date
1986-07-01 00:00:00pages
2364-70issue
7eissn
0270-7306issn
1098-5549journal_volume
6pub_type
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