Abstract:
:Expression of the recombinant human U1-70K protein in COS cells resulted in its rapid transport to the nucleus, even when binding to U1 RNA was debilitated. Deletion analysis of the U1-70K protein revealed the existence of two segments of the protein which were independently capable of nuclear localization. One nuclear localization signal (NLS) was mapped within the U1 RNA-binding domain and consists of two typically separated but interdependent elements. The major element of this NLS resides in structural loop 5 between the beta 4 strand and the alpha 2 helix of the folded RNA recognition motif. The C-terminal half of the U1-70K protein which was capable of nuclear entry contains two arginine-rich regions, which suggests the existence of a second NLS. Site-directed mutagenesis of the RNA recognition motif NLS demonstrated that the U1-70K protein can be transported independently of U1 RNA and that its association with the U1 small nuclear ribonucleoprotein particle can occur in the nucleus.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Romac JM,Graff DH,Keene JDdoi
10.1128/mcb.14.7.4662subject
Has Abstractpub_date
1994-07-01 00:00:00pages
4662-70issue
7eissn
0270-7306issn
1098-5549journal_volume
14pub_type
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