Origins and formation of histone methylation across the human cell cycle.

abstract：:Telomere stabilization is critical for tumorigenesis. A number of tumors and cell lines use a recombination-based mechanism, alternative lengthening of telomeres (ALT), to maintain telomere repeat arrays. Current data suggest that the mutation of p53 facilitates the activation of this pathway. In addition to its funct...

journal_title：Molecular and cellular biology

authors： Razak ZR,Varkonyi RJ,Kulp-McEliece M,Caslini C,Testa JR,Murphy ME,Broccoli D

更新日期：2004-07-01 00:00:00

abstract：:Nucleoporins mediate transport of macromolecules across the nuclear pore complex, yet the function of many individual nucleoporins is largely unresolved. To address this question, we depleted cells of the cytoplasmic nucleoporins Nup214/CAN and Nup358/RanBP2 by RNA interference. Depletion of Nup214 resulted in codeple...

journal_title：Molecular and cellular biology

authors： Hutten S,Kehlenbach RH

更新日期：2006-09-01 00:00:00

abstract：:All inbred strains of mice carry the Ren-1 structural gene, which encodes the renin-1 isozyme, the classical renin activity found in kidneys. In addition, some strains carry a second renin structural gene, Ren-2, which encodes the predominantly expressed submaxillary gland renin isozyme, renin-2. Ren-1 and Ren-2 exhib...

journal_title：Molecular and cellular biology

authors： Field LJ,Philbrick WM,Howles PN,Dickinson DP,McGowan RA,Gross KW

更新日期：1984-11-01 00:00:00

abstract：:Interleukin-6 (IL-6) and leukemia inhibitory factor (LIF), two multifunctional cytokines, recently have been identified as physiological inducers of hematopoietic cell differentiation which also induce terminal differentiation and growth arrest of the myeloblastic leukemic M1 cell line. In this work, it is shown that ...

journal_title：Molecular and cellular biology

authors： Hoffman-Liebermann B,Liebermann DA

更新日期：1991-05-01 00:00:00

abstract：:An accumulation in cells of unfolded proteins is believed to be the common signal triggering the induction of heat shock proteins (hsps). Accordingly, in Saccharomyces cerevisiae, inhibition of protein breakdown at 30 degrees C with the proteasome inhibitor MG132 caused a coordinate induction of many heat shock protei...

journal_title：Molecular and cellular biology

authors： Lee DH,Goldberg AL

更新日期：1998-01-01 00:00:00

abstract：:The cardiac troponin C (cTnC) gene produces identical transcripts in slow-twitch skeletal muscle and in heart muscle (R. Gahlmann, R. Wade, P. Gunning, and L. Kedes, J. Mol. Biol. 201:379-391, 1988). A separate gene encodes the fast-twitch skeletal muscle troponin C and is not expressed in heart muscle. We have used t...

journal_title：Molecular and cellular biology

authors： Christensen TH,Prentice H,Gahlmann R,Kedes L

更新日期：1993-11-01 00:00:00

abstract：:The metabolic lifetime of mRNA can be specified by specific cis-acting elements within mRNA. One type of element is an adenylate- and uridylate-rich element (ARE) found in the 3' untranslated region of many highly unstable mRNAs for mammalian early-response genes (ERGs). Among the better-characterized members of the E...

journal_title：Molecular and cellular biology

authors： Chen CY,Shyu AB

更新日期：1994-12-01 00:00:00

abstract：:Although the primary function of U1 snRNA is to define the 5' donor site of an intron, it can also block the accumulation of a specific RNA transcript when it binds to a donor sequence within its terminal exon. This work was initiated to investigate if this property of U1 snRNA could be exploited as an effective metho...

journal_title：Molecular and cellular biology

authors： Beckley SA,Liu P,Stover ML,Gunderson SI,Lichtler AC,Rowe DW

更新日期：2001-04-01 00:00:00

abstract：:By using a retrovirus-derived vector system, we generated derivatives of the human colon cancer cell line HT29 that stably overexpress a full-length cDNA encoding the beta 1 isoform of rat protein kinase C (PKC). Two of these cell lines, PKC6 and PKC7, displayed an 11- to 15-fold increase in PKC activity when compared...

journal_title：Molecular and cellular biology

authors： Choi PM,Tchou-Wong KM,Weinstein IB

更新日期：1990-09-01 00:00:00

abstract：:In the acellular slime mold Physarum polycephalum, the several hundred genes coding for rRNA are located on linear extrachromosomal DNA molecules of a discrete size, 60 kilobases. Each molecule contains two genes that are arranged in a palindromic fashion and separated by a central spacer region. We investigated how r...

journal_title：Molecular and cellular biology

authors： Ferris PJ,Vogt VM,Truitt CL

更新日期：1983-04-01 00:00:00

abstract：:A complex pattern of regulation of the cartilage matrix protein gene was revealed by transient expression experiments. A minimal promoter from positions -15 to +64 functioned in chondrocytes and fibroblasts. An enhancer located in the first intron exerted chondrocyte-specific stimulation on the minimal promoter activi...

journal_title：Molecular and cellular biology

authors： Kiss I,Bösze Z,Szabó P,Altanchimeg R,Barta E,Deák F

更新日期：1990-05-01 00:00:00

abstract：:Treatment of cells with tumor-promoting phorbol esters results in the activation but then depletion of phorbol ester-responsive protein kinase C (PKC) isoforms. The ubiquitin-proteasome pathway has been implicated in regulating the levels of many cellular proteins, including those involved in cell cycle control. We re...

journal_title：Molecular and cellular biology

authors： Lu Z,Liu D,Hornia A,Devonish W,Pagano M,Foster DA

更新日期：1998-02-01 00:00:00

abstract：:The Wnt signaling pathway plays a pivotal role in vertebrate early development and morphogenesis. Duplin (axis duplication inhibitor) interacts with beta-catenin and prevents its binding to Tcf, thereby inhibiting downstream Wnt signaling. Here we show that Duplin is expressed predominantly from early- to mid-stage mo...

journal_title：Molecular and cellular biology

authors： Nishiyama M,Nakayama K,Tsunematsu R,Tsukiyama T,Kikuchi A,Nakayama KI

更新日期：2004-10-01 00:00:00

abstract：:In the early phase of bone damage, low concentrations of the cytokine tumor necrosis factor alpha (TNF-α) favor osteoblast differentiation. In contrast, chronic high doses of the same cytokine contribute to bone loss, demonstrating opposite effects depending on its concentration and on the time of exposure. In the bon...

journal_title：Molecular and cellular biology

authors： Daniele S,Natali L,Giacomelli C,Campiglia P,Novellino E,Martini C,Trincavelli ML

更新日期：2017-03-31 00:00:00

abstract：:An ACG codon, which replaces the AUG codon used to initiate the synthesis of bacteriophage T7 gene 0.3 protein, was shown to function as a low-efficiency initiation codon in a wheat germ cell-free protein-synthesizing system. ...

journal_title：Molecular and cellular biology

authors： Anderson CW,Buzash-Pollert E

更新日期：1985-12-01 00:00:00

abstract：:The murine allele temperature-sensitive (ts) p53Val-135 encodes a ts p53 protein that behaves as a mutant polypeptide at 37 degrees C and as a wild-type polypeptide at 32 degrees C. This ts allele was introduced into the p53 nonproducer Friend erythroleukemia cell line DP16-1. The DP16-1 cell line was derived from the...

journal_title：Molecular and cellular biology

authors： Johnson P,Chung S,Benchimol S

更新日期：1993-03-01 00:00:00

abstract：:The GCN4 gene encodes a positive effector of amino acid biosynthetic genes in Saccharomyces cerevisiae. Genetic analysis has suggested that GCN4 is regulated by a hierarchy of interacting positive and negative effectors in response to amino acid starvation. Results presented here for a GCN4-lacZ gene fusion support th...

journal_title：Molecular and cellular biology

authors： Hinnebusch AG

更新日期：1985-09-01 00:00:00

abstract：:Cellular levels of diadenosine tetraphosphate (Ap4A) and adenosine tetraphospho-guanosine (Ap4G) were specifically measured during the cell cycle of Physarum polycephalum by a high-pressure liquid chromatographic method. Ap4A was also measured indirectly by a coupled phosphodiesterase-luciferase assay. No cell cycle-s...

journal_title：Molecular and cellular biology

authors： Garrison PN,Mathis SA,Barnes LD

更新日期：1986-04-01 00:00:00

abstract：:EGL-15 is a fibroblast growth factor receptor in the nematode Caenorhabditis elegans. Components that mediate EGL-15 signaling have been identified via mutations that confer a Clear (Clr) phenotype, indicative of hyperactivity of this pathway, or a suppressor-of-Clr (Soc) phenotype, indicative of reduced pathway activ...

journal_title：Molecular and cellular biology

authors： Schutzman JL,Borland CZ,Newman JC,Robinson MK,Kokel M,Stern MJ

更新日期：2001-12-01 00:00:00

abstract：:Genomic DNA in higher eucaryotic cells is organized into a series of loops, each of which may be affixed at its base to the nuclear matrix via a specific matrix attachment region (MAR). In this report, we describe the distribution of MARs within the amplified dihydrofolate reductase (DHFR) domain (amplicon) in the met...

journal_title：Molecular and cellular biology

authors： Dijkwel PA,Hamlin JL

更新日期：1988-12-01 00:00:00

abstract：:Phospholipase C-gamma2 (PLC-gamma2) plays an important role in B-cell signaling. Phosphorylation of various tyrosine residues of PLC-gamma2 has been implicated in regulation of its lipase activity. With the use of antibodies specific for each of the putative phosphorylation sites, we have now shown that PLC-gamma2 is ...

journal_title：Molecular and cellular biology

authors： Kim YJ,Sekiya F,Poulin B,Bae YS,Rhee SG

更新日期：2004-11-01 00:00:00

abstract：:The signal-transducing adaptor protein 2 (STAP-2) is a recently identified adaptor protein that contains a pleckstrin homology (PH) and Src homology 2 (SH2)-like domains, as well as a proline-rich domain in its C-terminal region. In previous studies, we demonstrated that STAP-2 binds to MyD88 and IKK-alpha or IKK-beta...

journal_title：Molecular and cellular biology

authors： Ikeda O,Sekine Y,Yasui T,Oritani K,Sugiyma K,Muromoto R,Ohbayashi N,Yoshimura A,Matsuda T

更新日期：2008-08-01 00:00:00

abstract：:Pax transactivation domain-interacting protein (PTIP, or PAXIP1) is required for mouse development and has been implicated in DNA damage responses and histone modification. However, the physiological roles of PTIP during embryogenesis remain unclear due to early embryonic lethality of null mutants. We describe two N-e...

journal_title：Molecular and cellular biology

authors： Mu W,Wang W,Schimenti JC

更新日期：2008-10-01 00:00:00

abstract：:The Polycomb group proteins are transcriptional repressors that are thought to act through multimeric nuclear complexes. We show that ph and Psc coprecipitate with Pc from nuclear extracts. We have analyzed the domains required for the association of Psc with ph and Pc by using the yeast two-hybrid system and an in vi...

journal_title：Molecular and cellular biology

authors： Kyba M,Brock HW

更新日期：1998-05-01 00:00:00

abstract：:DHTKD1, a part of 2-ketoadipic acid dehydrogenase complex, is involved in lysine and tryptophan catabolism. Mutations in DHTKD1 block the metabolic pathway and cause 2-aminoadipic and 2-oxoadipic aciduria (AMOXAD), an autosomal recessive inborn metabolic disorder. In addition, a nonsense mutation in DHTKD1 that we ide...

journal_title：Molecular and cellular biology

authors： Xu WY,Zhu H,Shen Y,Wan YH,Tu XD,Wu WT,Tang L,Zhang HX,Lu SY,Jin XL,Fei J,Wang ZG

更新日期：2018-06-14 00:00:00

abstract：:Recognition of gene promoters by RNA polymerase II is mediated by general transcription factor IID (TFIID), which has been thought to be a static complex and to play a passive role in the regulation of gene expression under the instruction of gene-specific transcription factors. Here we show that transforming growth f...

journal_title：Molecular and cellular biology

authors： Nakagawa T,Hosogane M,Nakagawa M,Morohoshi A,Funayama R,Nakayama K

更新日期：2018-02-12 00:00:00

abstract：:The Saccharomyces cerevisiae PGK (phosphoglycerate kinase) gene encodes one of the most abundant mRNA and protein species in the cell. To identify the promoter sequences required for the efficient expression of PGK, we undertook a detailed internal deletion analysis of the 5' noncoding region of the gene. Our analysis...

journal_title：Molecular and cellular biology

authors： Ogden JE,Stanway C,Kim S,Mellor J,Kingsman AJ,Kingsman SM

更新日期：1986-12-01 00:00:00

abstract：:In Saccharomyces cerevisiae, transcriptional activation mediated by the GAL4 regulatory protein is repressed in the absence of galactose by the binding of the GAL80 protein, an interaction that requires the carboxy-terminal 28 amino acids of GAL4. The homolog of GAL4 from Kluyveromyces lactis, LAC9, activates transcri...

journal_title：Molecular and cellular biology

authors： Salmeron JM Jr,Langdon SD,Johnston SA

更新日期：1989-07-01 00:00:00

abstract：:p105 (NFKB1) acts in a dual way as a cytoplasmic IkappaB molecule and as the source of the NF-kappaB p50 subunit upon processing. p105 can form various heterodimers with other NF-kappaB subunits, including its own processing product, p50, and these complexes are signal responsive. Signaling through the IkappaB kinase ...

journal_title：Molecular and cellular biology

authors： Heissmeyer V,Krappmann D,Hatada EN,Scheidereit C

更新日期：2001-02-01 00:00:00

abstract：:In CBFbeta-SMMHC, core binding factor beta (CBFbeta) is fused to the alpha-helical rod domain of smooth muscle myosin heavy chain (SMMHC). We generated Ba/F3 hematopoietic cells expressing a CBFbeta-SMMHC variant lacking 28 amino acids homologous to the assembly competence domain (ACD) required for multimerization of ...

journal_title：Molecular and cellular biology

authors： Kummalue T,Lou J,Friedman AD

更新日期：2002-12-01 00:00:00