Positive selection of FLP-mediated unequal sister chromatid exchange products in mammalian cells.

abstract：:Drosophila hsp70 genes have an RNA polymerase II molecule paused at their 5' ends in uninduced cells. In this study we have shown that this pausing also occurs on other heat shock and constitutively expressed genes. We propose that a rate-limiting step in early elongation occurs in many Drosophila genes and may be a t...

journal_title：Molecular and cellular biology

authors： Rougvie AE,Lis JT

更新日期：1990-11-01 00:00:00

abstract：:Expression of the granulocyte-macrophage colony-stimulating factor (GM-CSF) gene in T cells is activated by the combination of phorbol ester (phorbol myristate acetate) and calcium ionophore (A23187), which mimic antigen stimulation through the T-cell receptor. We have previously shown that a fragment containing bp -9...

journal_title：Molecular and cellular biology

authors： Masuda ES,Tokumitsu H,Tsuboi A,Shlomai J,Hung P,Arai K,Arai N

更新日期：1993-12-01 00:00:00

abstract：:Neurite outgrowth is key to the formation of functional circuits during neuronal development. Neurotrophins, including nerve growth factor (NGF), increase neurite outgrowth in part by altering the function and expression of Ca(2+)-permeable cation channels. Here we report that transient receptor potential vanilloid 2 ...

journal_title：Molecular and cellular biology

authors： Cohen MR,Johnson WM,Pilat JM,Kiselar J,DeFrancesco-Lisowitz A,Zigmond RE,Moiseenkova-Bell VY

更新日期：2015-12-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, meiosis and spore formation require the induction of sporulation-specific genes. Two genes are thought to activate the sporulation program: IME1 and IME2 (inducer of meiosis). Both genes are induced upon entry into meiosis, and IME1 is required for IME2 expression. We report here...

journal_title：Molecular and cellular biology

authors： Mitchell AP,Driscoll SE,Smith HE

更新日期：1990-05-01 00:00:00

abstract：:Tumor suppressor genes regulate the antiviral host defense through molecular mechanisms that are not yet well explored. Here, we show that the tumor suppressor retinoblastoma (Rb) protein positively regulates Toll-like receptor 3 (TLR3) expression, the sensing receptor for viral double-stranded RNA and poly(I · C). TL...

journal_title：Molecular and cellular biology

authors： Taura M,Suico MA,Koyama K,Komatsu K,Miyakita R,Matsumoto C,Kudo E,Kariya R,Goto H,Kitajima S,Takahashi C,Shuto T,Nakao M,Okada S,Kai H

更新日期：2012-04-01 00:00:00

abstract：:The Drosophila gap gene knirps (kni) is required for abdominal segmentation. It encodes a steroid/thyroid orphan receptor-type transcription factor which is distributed in a broad band of nuclei in the posterior region of the blastoderm. To identify essential domains of the kni protein (KNI), we cloned and sequenced t...

journal_title：Molecular and cellular biology

authors： Gerwin N,La Rosée A,Sauer F,Halbritter HP,Neumann M,Jäckle H,Nauber U

更新日期：1994-12-01 00:00:00

abstract：:Receptor tyrosine kinases (RTKs) play distinct roles in multiple biological systems. Many RTKs transmit similar signals, raising questions about how specificity is achieved. One potential mechanism for RTK specificity is control of the magnitude and kinetics of activation of downstream pathways. We have found that the...

journal_title：Molecular and cellular biology

authors： Zhang SQ,Tsiaras WG,Araki T,Wen G,Minichiello L,Klein R,Neel BG

更新日期：2002-06-01 00:00:00

abstract：:IkappaB alpha retains the transcription factor NF-kappaB in the cytoplasm, thus inhibiting its function. Various stimuli inactivate IkappaB alpha by triggering phosphorylation of the N-terminal residues Ser32 and Ser36. Phosphorylation of both serines is demonstrated directly by phosphopeptide mapping utilizing calpai...

journal_title：Molecular and cellular biology

authors： Brown K,Franzoso G,Baldi L,Carlson L,Mills L,Lin YC,Gerstberger S,Siebenlist U

更新日期：1997-06-01 00:00:00

abstract：:The myeloperoxidase (MPO) and neutrophil elastase genes are expressed specifically in immature myeloid cells. The integrity of a polyomavirus enhancer core sequence, 5'-AACCACA-3', is critical to the activity of the murine MPO proximal enhancer. This element binds two species, myeloid nuclear factors 1 alpha and 1 bet...

journal_title：Molecular and cellular biology

authors： Nuchprayoon I,Meyers S,Scott LM,Suzow J,Hiebert S,Friedman AD

更新日期：1994-08-01 00:00:00

abstract：:We have previously described an isolated kinetoplast system from Crithidia fasciculata capable of ATP-dependent replication of kinetoplast DNA minicircles (L. Birkenmeyer and D.S. Ray, J. Biol. Chem. 261: 2362-2368, 1986). We present here the identification of two new minicircle species observed in short pulse-labelin...

journal_title：Molecular and cellular biology

authors： Sheline C,Melendy T,Ray DS

更新日期：1989-01-01 00:00:00

abstract：:ZO-1, ZO-2, and ZO-3 are closely related MAGUK family proteins that localize at the cytoplasmic surface of tight junctions (TJs). ZO-1 and ZO-2 are expressed in both epithelia and endothelia, whereas ZO-3 is exclusively expressed in epithelia. In spite of intensive studies of these TJ MAGUKs, our knowledge of their fu...

journal_title：Molecular and cellular biology

authors： Adachi M,Inoko A,Hata M,Furuse K,Umeda K,Itoh M,Tsukita S

更新日期：2006-12-01 00:00:00

abstract：:Signal transducer and activator of transcription 6 (Stat6) and NF-kappaB are widely distributed transcription factors which are induced by different stimuli and bind to distinct DNA sequence motifs. Interleukin-4 (IL-4), which activates Stat6, synergizes with activators of NF-kappaB to induce IL-4-responsive genes, bu...

journal_title：Molecular and cellular biology

authors： Shen CH,Stavnezer J

更新日期：1998-06-01 00:00:00

abstract：:We have isolated a full-length mouse cDNA encoding a lysine-rich protein of 1,131 amino acids with a calculated molecular mass of 126 kDa. The protein binds in a sequence-unspecific manner to DNA, is localized exclusively in the nucleus, and contains a putative ATP binding site and a stretch of 80 amino acids with hom...

journal_title：Molecular and cellular biology

authors： Luckow B,Bunz F,Stillman B,Lichter P,Schütz G

更新日期：1994-03-01 00:00:00

abstract：:Plasmid DNA substrates were X-irradiated and injected into the nuclei of Xenopus laevis oocytes. After incubation for 20 h, DNA was recovered from the oocytes and analyzed simultaneously for repair and for intermolecular homologous recombination by electrophoresis and bacterial transformation. Oocyte-mediated repair o...

journal_title：Molecular and cellular biology

authors： Sweigert SE,Carroll D

更新日期：1990-11-01 00:00:00

abstract：:At least two separate but interdependent events are required to attain autonomous growth as a consequence of ectopic expression of the multilineage colony-stimulating factor gene in hematopoietic progenitor cells. The rate at which the second event occurs is more than 3 orders of magnitude higher in precursor cell lin...

journal_title：Molecular and cellular biology

authors： Laker C,Kluge N,Stocking C,Just U,Franz MJ,Ostertag W,DeLamarter JF,Dexter M,Spooncer E

更新日期：1989-12-01 00:00:00

abstract：:Cancer cells often heavily depend on the ubiquitin-proteasome system (UPS) for their growth and survival. Irrespective of their strong dependence on the proteasome activity, cancer cells, except for multiple myeloma, are mostly resistant to proteasome inhibitors. A major cause of this resistance is the proteasome boun...

journal_title：Molecular and cellular biology

authors： Sekine H,Okazaki K,Kato K,Alam MM,Shima H,Katsuoka F,Tsujita T,Suzuki N,Kobayashi A,Igarashi K,Yamamoto M,Motohashi H

更新日期：2018-08-15 00:00:00

abstract：:Wild-type strains of Saccharomyces cerevisiae exhibit mitotic recombination between the chimeric plasmid TLC-1 and the endogenous 2mu circle that involves sequence homologies between the two plasmids that are not acted on by the 2mu circle site-specific recombination system. This generalized recombination can be detec...

journal_title：Molecular and cellular biology

authors： Whiteway MS,Ahmed A

更新日期：1984-01-01 00:00:00

abstract：:Upon the addition of different growth factors and cytokines, the Gab1 docking protein is tyrosine phosphorylated and in turn activates different signaling pathways. On the basis of the large body of evidence concerning cross talk between the signaling pathways activated by growth factors and oxidative stress, we decid...

journal_title：Molecular and cellular biology

authors： Holgado-Madruga M,Wong AJ

更新日期：2003-07-01 00:00:00

abstract：:The myeloperoxidase (MPO) gene is transcribed specifically in immature myeloid cells and is regulated in part by a 414-bp proximal enhancer. Mutation of a core binding factor (CBF)-binding site at -288 decreased enhancer activity 30-fold in 32D cl3 myeloid cells cultured in granulocyte colony-stimulating factor (G-CSF...

journal_title：Molecular and cellular biology

authors： Britos-Bray M,Friedman AD

更新日期：1997-09-01 00:00:00

abstract：:To study the relationship between oncogenesis by v-src and normal cellular signalling pathways, we determined the effects of v-src on 3T3-TNR9 cells, a Swiss 3T3 variant which does not respond mitogenically to tumor promoters such as 12-O-tetradecanoyl-phorbol-13-acetate (TPA). We found that src was unable to transfor...

journal_title：Molecular and cellular biology

authors： Nori M,Shawver LK,Weber MJ

更新日期：1990-08-01 00:00:00

abstract：:DHCR24/seladin-1, a crucial enzyme in sterol synthesis, is of lower abundance in brain areas affected by Alzheimer's disease. While high levels of DHCR24/seladin-1 exert antiapoptotic function by conferring resistance against oxidative stress, the molecular mechanism for this protective effect is not fully understood....

journal_title：Molecular and cellular biology

authors： Kuehnle K,Crameri A,Kälin RE,Luciani P,Benvenuti S,Peri A,Ratti F,Rodolfo M,Kulic L,Heppner FL,Nitsch RM,Mohajeri MH

更新日期：2008-01-01 00:00:00

abstract：:We have recently reported that the yeast chromatin-remodeling factor Swi1 can exist as a prion, [SWI(+)], demonstrating a link between prionogenesis and global transcriptional regulation. To shed light on how the Swi1 conformational switch influences Swi1 function and to define the sequence and structural requirements...

journal_title：Molecular and cellular biology

authors： Du Z,Crow ET,Kang HS,Li L

更新日期：2010-10-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the intracellular concentration of trehalose increases rapidly in response to many environmental stresses, including heat shock. These high trehalose levels have been correlated with tolerance to adverse conditions and led to the model that trehalose functions as a chemical cochaperone. He...

journal_title：Molecular and cellular biology

authors： Conlin LK,Nelson HC

更新日期：2007-02-01 00:00:00

abstract：:The Rex protein of human T-cell leukemia virus type 1, like the functionally equivalent Rev protein of human immunodeficiency virus type 1, contains a leucine-rich activation domain that specifically interacts with the human nucleoporin-like Rab/hRIP cofactor. Here, this Rex sequence is shown to function also as a pro...

journal_title：Molecular and cellular biology

authors： Bogerd HP,Fridell RA,Benson RE,Hua J,Cullen BR

更新日期：1996-08-01 00:00:00

abstract：:Interleukin-6 (IL-6) and leukemia inhibitory factor (LIF), two multifunctional cytokines, recently have been identified as physiological inducers of hematopoietic cell differentiation which also induce terminal differentiation and growth arrest of the myeloblastic leukemic M1 cell line. In this work, it is shown that ...

journal_title：Molecular and cellular biology

authors： Hoffman-Liebermann B,Liebermann DA

更新日期：1991-05-01 00:00:00

abstract：:The erythropoietin (Epo) gene is under tissue-specific inducible regulation. Because the kidney is the primary EPO-producing tissue in adults, impaired EPO production in chronic kidney disorders results in serious renal anemia. The Epo gene contains a liver-specific enhancer in the 3' region, but the kidney-specific e...

journal_title：Molecular and cellular biology

authors： Hirano I,Suzuki N,Yamazaki S,Sekine H,Minegishi N,Shimizu R,Yamamoto M

更新日期：2017-02-01 00:00:00

abstract：:The 3' ends of metazoan histone mRNAs are generated by specialized processing machinery that cleaves downstream of a conserved stem-loop structure. To examine how this reaction might be influenced by transcription, we used a Drosophila melanogaster in vitro system that supports both processes. In this system the compl...

journal_title：Molecular and cellular biology

authors： Adamson TE,Price DH

更新日期：2003-06-01 00:00:00

abstract：:A recent analysis of gene expression in renal cell carcinoma cells led to the identification of mRNAs whose translation was dependent on the presence of the von Hippel-Lindau (VHL) tumor suppressor gene product, pVHL. Here, we investigate the finding that pVHL-expressing RCC cells (VHL(+)) exhibited elevated levels of...

journal_title：Molecular and cellular biology

authors： Galbán S,Martindale JL,Mazan-Mamczarz K,López de Silanes I,Fan J,Wang W,Decker J,Gorospe M

更新日期：2003-10-01 00:00:00

abstract：:The onc gene (v-fes) of the acutely transforming feline sarcoma virus (Snyder-Theilen strain) has homologous cellular sequences (c-fes) in all vertebrate species, including humans. We isolated from a human DNA library recombinant phages containing overlapping c-fes sequences. The human c-fes locus spans a region of 3....

journal_title：Molecular and cellular biology

authors： Franchini G,Gelmann EP,Dalla-Favera R,Gallo RC,Wong-Staal F

更新日期：1982-08-01 00:00:00

abstract：:The pathway of vesicular stomatitis virus N protein from synthesis to assembly into capsids was studied by use of detergent extraction of infected HeLa cells together with protein cross-linking. One half of the newly synthesized N protein was extracted with the soluble cell proteins and, when cross-linked, never forme...

journal_title：Molecular and cellular biology

authors： Chatterjee PK,Cervera MM,Penman S

更新日期：1984-10-01 00:00:00