Mutational analysis of the hsp70-interacting protein Hip.

abstract：:Calcineurin (Cn) is a calcium-activated serine/threonine protein phosphatase that is broadly implicated in diverse cellular processes, including the regulation of gene expression. During skeletal muscle differentiation, Cn activates the nuclear factor of activated T-cell (NFAT) transcription factor but also promotes d...

journal_title：Molecular and cellular biology

authors： Witwicka H,Nogami J,Syed SA,Maehara K,Padilla-Benavides T,Ohkawa Y,Imbalzano AN

更新日期：2019-09-11 00:00:00

abstract：:In response to ionizing radiation (IR), the tumor suppressor p53 is stabilized and promotes either cell cycle arrest or apoptosis. Chk2 activated by IR contributes to this stabilization, possibly by direct phosphorylation. Like p53, Chk2 is mutated in patients with Li-Fraumeni syndrome. Since the ataxia telangiectasia...

journal_title：Molecular and cellular biology

authors： Hirao A,Cheung A,Duncan G,Girard PM,Elia AJ,Wakeham A,Okada H,Sarkissian T,Wong JA,Sakai T,De Stanchina E,Bristow RG,Suda T,Lowe SW,Jeggo PA,Elledge SJ,Mak TW

更新日期：2002-09-01 00:00:00

abstract：:The tumor suppressor gene PTEN is a phosphoinositide phosphatase that is inactivated by deletion and/or mutation in diverse human tumors. Wild-type PTEN is expressed both in the cytoplasm and nucleus in normal cells, with a preferential nuclear localization in differentiated or resting cells. To elucidate the relation...

journal_title：Molecular and cellular biology

authors： Liu JL,Sheng X,Hortobagyi ZK,Mao Z,Gallick GE,Yung WK

更新日期：2005-07-01 00:00:00

abstract：:AIRE is a transcriptional activator that directs the ectopic expression of many tissue-specific genes in medullary thymic epithelial cells, which plays an important role in the negative selection of autoreactive T cells. However, its mechanism of action remains poorly understood. In this study, we found that AIRE regu...

journal_title：Molecular and cellular biology

authors： Oven I,Brdicková N,Kohoutek J,Vaupotic T,Narat M,Peterlin BM

更新日期：2007-12-01 00:00:00

abstract：:Hybridization to synthetic oligonucleotides representing conserved regions in the promoter and first intron of several vertebrate beta-actin genes was used to discriminate between what appears to be a single functional beta-actin gene and numerous pseudogenes in the mouse genome. Sequences derived from the 5' end of t...

journal_title：Molecular and cellular biology

authors： Elder PK,French CL,Subramaniam M,Schmidt LJ,Getz MJ

更新日期：1988-01-01 00:00:00

abstract：:Cyclic AMP-responsive element-binding protein 3-like 3, hepatocyte specific (CREBH), is a hepatic transcription factor that functions as a key regulator of energy homeostasis. Here, we defined a regulatory CREBH posttranslational modification process, namely, lysine-specific acetylation, and its functional involvement...

journal_title：Molecular and cellular biology

authors： Kim H,Mendez R,Chen X,Fang D,Zhang K

更新日期：2015-12-01 00:00:00

abstract：:T beta R-II (transforming growth factor beta [TGF-beta] type II receptor) is a transmembrane serine/threonine kinase that acts as the primary TGF-beta receptor. Ligand binding to T beta R-II leads to the recruitment and phosphorylation of T beta R-I, a distantly related transmembrane kinase that acts as a downstream s...

journal_title：Molecular and cellular biology

authors： Cárcamo J,Zentella A,Massagué J

更新日期：1995-03-01 00:00:00

abstract：:TEL-JAK2 fusion proteins, which are a result of t(9;12)(p24;p13) translocations associated with human leukemia, activate Stat5 in vitro and in vivo and cause a myelo- and lymphoproliferative disease in a murine bone marrow transplant model. We report that Socs-1, a member of the SOCS family of endogenous inhibitors of...

journal_title：Molecular and cellular biology

authors： Frantsve J,Schwaller J,Sternberg DW,Kutok J,Gilliland DG

更新日期：2001-05-01 00:00:00

abstract：:Protein ubiquitination plays a key role in the regulation of a variety of DNA repair mechanisms. Protein ubiquitination is controlled by the coordinate activity of ubiquitin ligases and deubiquitinating enzymes (DUBs). The deubiquitinating enzyme USP1 regulates DNA repair and the Fanconi anemia pathway through its ass...

journal_title：Molecular and cellular biology

authors： Murai J,Yang K,Dejsuphong D,Hirota K,Takeda S,D'Andrea AD

更新日期：2011-06-01 00:00:00

abstract：:We investigated the role of cytoplasmic and anchor domains of type II glycoproteins in intracellular transport, oligomerization, and endocytosis by expressing the wild-type and chimeric genes in mammalian cells. Chimeric genes were constructed by exchanging the DNA segments that encode the cytoplasmic and anchor domai...

journal_title：Molecular and cellular biology

authors： Kundu A,Jabbar MA,Nayak DP

更新日期：1991-05-01 00:00:00

abstract：:Hepatocyte growth factor (HGF), a cytokine with multiple functions, exhibits cell-type-specific as well as cytokine- and steroid hormone-regulated expression. The HGF gene is known to be expressed predominately in mesenchymal but not in epithelial cells. In this study, we report the identification of a cell-type-speci...

journal_title：Molecular and cellular biology

authors： Liu Y,Beedle AB,Lin L,Bell AW,Zarnegar R

更新日期：1994-11-01 00:00:00

abstract：:The interleukin-2 receptor (IL-2R) beta chain (IL-2R beta) is an essential signaling component of high- and intermediate-affinity IL-2Rs. Our laboratory previously reported that a DNA fragment containing 857 bp of 5'-flanking sequence of the human IL-2R beta gene exhibited promoter activity. We have now further charac...

journal_title：Molecular and cellular biology

authors： Lin JX,Bhat NK,John S,Queale WS,Leonard WJ

更新日期：1993-10-01 00:00:00

abstract：:In order to identify catalytically important amino acid changes within the second-largest subunit of yeast RNA polymerase III, we mutagenized selected regions of its gene (RET1) and devised in vivo assays for both increased and decreased transcription termination by this enzyme. Using as the reporter gene a mutant SUP...

journal_title：Molecular and cellular biology

authors： Shaaban SA,Krupp BM,Hall BD

更新日期：1995-03-01 00:00:00

abstract：:Quantitative immunoelectron microscopy and subcellular fractionation established the site of endoplasmic reticulum (ER)-Golgi transport arrest induced by the phosphatase inhibitor okadaic acid (OA). OA induced the disappearance of transitional element tubules and accumulation of the anterograde-transported Chandipura ...

journal_title：Molecular and cellular biology

authors： Pryde JG,Farmaki T,Lucocq JM

更新日期：1998-02-01 00:00:00

abstract：:Synonymous single nucleotide polymorphisms (SNPs) within a transcript's coding region produce no change in the amino acid sequence of the protein product and are therefore intuitively assumed to have a neutral effect on protein function. We report that two common variants of high-temperature requirement A1 (HTRA1) tha...

journal_title：Molecular and cellular biology

authors： Jacobo SM,Deangelis MM,Kim IK,Kazlauskas A

更新日期：2013-05-01 00:00:00

abstract：:The UvsW protein of bacteriophage T4 is involved in many aspects of phage DNA metabolism, including repair, recombination, and recombination-dependent replication. UvsW has also been implicated in the repression of origin-dependent replication at late times of infection, when UvsW is normally synthesized. Two well-cha...

journal_title：Molecular and cellular biology

authors： Dudas KC,Kreuzer KN

更新日期：2001-04-01 00:00:00

abstract：:Although phosphoinositide 3-kinase (PI 3-kinase) is essential for cell cycle progression, the molecular mechanisms that regulate its diverse biological effects are poorly understood. We demonstrate here that Rb, a key regulator of cell cycle progression, associates with p55 kDa (p55alpha and p55gamma) regulatory subun...

journal_title：Molecular and cellular biology

authors： Xia X,Cheng A,Akinmade D,Hamburger AW

更新日期：2003-03-01 00:00:00

abstract：:Strong evidence indicates that endosome-localized epidermal growth factor receptor (EGFR) plays an important role in cell signaling. However, elimination of endosomal signaling does not attenuate EGF-induced physiological outcomes, arguing against physiological relevance. Recently we established a system to specifical...

journal_title：Molecular and cellular biology

authors： Pennock S,Wang Z

更新日期：2003-08-01 00:00:00

abstract：:Three subtypes of retinoic acid receptors (RAR), termed RAR alpha, RAR beta, and RAR gamma, have been described. They are composed of different structural domains, including distinct domains for DNA and ligand binding. RARs specifically bind all-trans-retinoic acid (RA), 9-cis-RA, and retinoid analogs. In this study, ...

journal_title：Molecular and cellular biology

authors： Lamour FP,Lardelli P,Apfel CM

更新日期：1996-10-01 00:00:00

abstract：:In Saccharomyces cerevisiae, COX5a and COX5b encode two distinct forms of cytochrome c oxidase subunit V, Va and Vb, respectively. To determine the relative contribution of COX5a and COX5b to cytochrome c oxidase function, we have disrupted each gene. Cytochrome c oxidase activity levels and respiration rates of strai...

journal_title：Molecular and cellular biology

authors： Trueblood CE,Poyton RO

更新日期：1987-10-01 00:00:00

abstract：:Abundant approximately 28-nucleotide RNAs that are thought to direct histone H3 lysine 9 (H3K9) methylation and promote the elimination of nearly 15 Mbp of DNA from the developing somatic genome are generated during Tetrahymena thermophila conjugation. To identify the protein(s) that generates these small RNAs, we stu...

journal_title：Molecular and cellular biology

authors： Malone CD,Anderson AM,Motl JA,Rexer CH,Chalker DL

更新日期：2005-10-01 00:00:00

abstract：:Combined deficiencies of poly(ADP)ribosyl polymerase 1 (PARP1) and ataxia telangiectasia mutated (ATM) result in synthetic lethality and, in the mouse, early embryonic death. Here, we investigated the genetic requirements for this lethality via analysis of mice deficient for PARP1 and either of two ATM-regulated DNA d...

journal_title：Molecular and cellular biology

authors： Orsburn B,Escudero B,Prakash M,Gesheva S,Liu G,Huso DL,Franco S

更新日期：2010-05-01 00:00:00

abstract：:We have previously described an isolated kinetoplast system from Crithidia fasciculata capable of ATP-dependent replication of kinetoplast DNA minicircles (L. Birkenmeyer and D.S. Ray, J. Biol. Chem. 261: 2362-2368, 1986). We present here the identification of two new minicircle species observed in short pulse-labelin...

journal_title：Molecular and cellular biology

authors： Sheline C,Melendy T,Ray DS

更新日期：1989-01-01 00:00:00

abstract：:To modulate transcription, regulatory factors communicate with basal transcription factors and/or RNA polymerases in a variety of ways. Previously, it has been reported that RNA polymerase II subunit 5 (RPB5) is one of the targets of hepatitis B virus X protein (HBx) and that both HBx and RPB5 specifically interact wi...

journal_title：Molecular and cellular biology

authors： Dorjsuren D,Lin Y,Wei W,Yamashita T,Nomura T,Hayashi N,Murakami S

更新日期：1998-12-01 00:00:00

abstract：:Pref-1/Dlk1 is made as an epidermal growth factor (EGF) repeat-containing transmembrane protein but is cleaved by tumor necrosis factor alpha converting enzyme (TACE) to generate a biologically active soluble form. Soluble Pref-1 inhibits adipocyte differentiation through the activation of extracellular signal-regulat...

journal_title：Molecular and cellular biology

authors： Wang Y,Zhao L,Smas C,Sul HS

更新日期：2010-07-01 00:00:00

abstract：:p53 promotes apoptosis in response to death stimuli by transactivation of target genes and by transcription-independent mechanisms. We recently showed that wild-type p53 rapidly translocates to mitochondria in response to multiple death stimuli in cultured cells. Mitochondrial p53 physically interacts with antiapoptot...

journal_title：Molecular and cellular biology

authors： Erster S,Mihara M,Kim RH,Petrenko O,Moll UM

更新日期：2004-08-01 00:00:00

abstract：:Axin is a recently identified protein encoded by the fused locus in mice that is required for normal vertebrate axis formation. We have defined a 25-amino-acid sequence in axin that comprises the glycogen synthase kinase 3beta (GSK-3beta) interaction domain (GID). In contrast to full-length axin, which has been shown ...

journal_title：Molecular and cellular biology

authors： Hedgepeth CM,Deardorff MA,Rankin K,Klein PS

更新日期：1999-10-01 00:00:00

abstract：:V(D)J recombination requires binding and synapsis of a complementary (12/23) pair of recombination signal sequences (RSSs) by the RAG1 and RAG2 proteins, aided by a high-mobility group protein, HMG1 or HMG2. Double-strand DNA cleavage within this synaptic, or paired, complex is thought to involve DNA distortion or mel...

journal_title：Molecular and cellular biology

authors： Ciubotaru M,Schatz DG

更新日期：2004-10-01 00:00:00

abstract：:Using PCR-coupled subtractive screening-representational difference analysis, we have cloned a novel gene from AML1-ETO knockin mice. This gene is highly expressed in the yolk sac and fetal liver of the knockin mice. Nucleotide sequence analysis indicates that its cDNA contains an 1,107-bp open reading frame encoding ...

journal_title：Molecular and cellular biology

authors： Liu LQ,Ilaria R Jr,Kingsley PD,Iwama A,van Etten RA,Palis J,Zhang DE

更新日期：1999-04-01 00:00:00

abstract：:We have developed a high-frequency cotransformation system which is useful in introducing nonreplicating circular DNA plasmids into the fission yeast Schizosaccharomyces pombe. This system depends on two factors: the ability of the ural-complementing helper plasmids pFYM2 and pFYM225 to propagate autonomously in S. po...

journal_title：Molecular and cellular biology

authors： Sakai K,Sakaguchi J,Yamamoto M

更新日期：1984-04-01 00:00:00