Differential effectiveness of yeast cytochrome c oxidase subunit genes results from differences in expression not function.

abstract：:Recombination and synapsis of homologous chromosomes are hallmarks of meiosis in many organisms. Meiotic recombination is initiated by Spo11-induced DNA double-strand breaks (DSBs), whereas chromosome synapsis is mediated by a tripartite structure named the synaptonemal complex (SC). Previously, we proposed that buddi...

journal_title：Molecular and cellular biology

authors： Cheng YH,Chuang CN,Shen HJ,Lin FM,Wang TF

更新日期：2013-08-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the intracellular concentration of trehalose increases rapidly in response to many environmental stresses, including heat shock. These high trehalose levels have been correlated with tolerance to adverse conditions and led to the model that trehalose functions as a chemical cochaperone. He...

journal_title：Molecular and cellular biology

authors： Conlin LK,Nelson HC

更新日期：2007-02-01 00:00:00

abstract：:With the murine retinoblastoma (RB) cDNA, a series of RB mutants were expressed in COS-1 cells and the pRB products were assessed for their ability (i) to bind to large T antigen (large T), (ii) to become modified by phosphorylation, and (iii) to localize in the nucleus. All point mutations and deletions introduced in...

journal_title：Molecular and cellular biology

authors： Hamel PA,Cohen BL,Sorce LM,Gallie BL,Phillips RA

更新日期：1990-12-01 00:00:00

abstract：:Two signals activate meiosis in yeast: starvation and expression of the a1 and alpha 2 products of the mating-type locus. Prior studies suggest that these signals stimulate expression of an activator of meiosis, the IME1 (inducer of meiosis) product. We have cloned a gene, IME2, with properties similar to those of IME...

journal_title：Molecular and cellular biology

authors： Smith HE,Mitchell AP

更新日期：1989-05-01 00:00:00

abstract：:Treatment of K562 cells, a human erythroleukemia cell line, with desferrioxamine raised the levels of the receptor for transferrin (Tf) two- to threefold over that of the control cells. The levels of receptor were reduced by at least 50 and 35% of that of the control in cells treated with diferric Tf and ferric ammoni...

journal_title：Molecular and cellular biology

authors： Rao KK,Shapiro D,Mattia E,Bridges K,Klausner R

更新日期：1985-04-01 00:00:00

abstract：:Only a fraction of retroviral primary transcripts are spliced to subgenomic mRNAs; the unspliced transcripts are transported to the cytoplasm for packaging into virions and for translation of the gag and pol genes. We identified cis-acting sequences within the gag gene of Rous sarcoma virus (RSV) which negatively regu...

journal_title：Molecular and cellular biology

authors： Arrigo S,Beemon K

更新日期：1988-11-01 00:00:00

abstract：:Protein kinase C theta (PKC theta) is unique among PKC isozymes in its translocation to the center of the immune synapse in T cells and its unique downstream signaling. Here we show that the hematopoietic protein tyrosine phosphatase (HePTP) also accumulates in the immune synapse in a PKC theta-dependent manner upon a...

journal_title：Molecular and cellular biology

authors： Nika K,Charvet C,Williams S,Tautz L,Bruckner S,Rahmouni S,Bottini N,Schoenberger SP,Baier G,Altman A,Mustelin T

更新日期：2006-03-01 00:00:00

abstract：:TFIID, comprising the TATA box binding protein (TBP) and 13 TBP-associated factors (TAFs), plays a role in nucleation in the assembly of the RNA polymerase II preinitiation complexes on protein-encoding genes. TAFs are shared among other transcription regulatory complexes (e.g., SAGA, TBP-free TAF-containing complex [...

journal_title：Molecular and cellular biology

authors： Soutoglou E,Demény MA,Scheer E,Fienga G,Sassone-Corsi P,Tora L

更新日期：2005-05-01 00:00:00

abstract：:The E1a gene of adenovirus encodes two proteins, 289 and 243 amino acids long, which have positive (transactivator) and negative (enhancer repressor) RNA polymerase II transcriptional regulatory properties and cell transformation activities including cooperation with an activated ras gene. The E1a transforming functio...

journal_title：Molecular and cellular biology

authors： Velcich A,Ziff E

更新日期：1988-05-01 00:00:00

abstract：:Zinc finger genes encode proteins that act as transcription factors. The myeloid zinc finger 1 (MZF1) gene encodes a zinc finger protein with two DNA-binding domains that recognize two distinct consensus sequences, is preferentially expressed in hematopoietic cells, and may be involved in the transcriptional regulatio...

journal_title：Molecular and cellular biology

authors： Perrotti D,Melotti P,Skorski T,Casella I,Peschle C,Calabretta B

更新日期：1995-11-01 00:00:00

abstract：:Telomerase extends chromosome ends by the synthesis of tandem simple-sequence repeats. Studies of minimal recombinant telomerase ribonucleoprotein (RNP) reconstituted in vitro have revealed sequences within the telomerase RNA subunit (TER) that are required to establish its internal template and other unique features ...

journal_title：Molecular and cellular biology

authors： Cunningham DD,Collins K

更新日期：2005-06-01 00:00:00

abstract：:Genomic instability, including the ability to undergo gene amplification, is a hallmark of neoplastic cells. Similar to normal cells, "nonpermissive" REF52 cells do not develop resistance to N-(phosphonacetyl)-L-aspartate (PALA), an inhibitor of the synthesis of pyrimidine nucleotides, through amplification of cad, th...

journal_title：Molecular and cellular biology

authors： Chernova OB,Chernov MV,Ishizaka Y,Agarwal ML,Stark GR

更新日期：1998-01-01 00:00:00

abstract：:The immunoglobulin M heavy-chain locus contains two poly(A) sites which are alternatively expressed during B-cell differentiation. Despite its promoter proximal location, the secretory poly(A) site is not expressed in undifferentiated cells. Crucial to the activation of the secretory poly(A) site during B-cell differe...

journal_title：Molecular and cellular biology

authors： Phillips C,Pachikara N,Gunderson SI

更新日期：2004-07-01 00:00:00

abstract：:The variety of environmental stresses is probably the major challenge imposed on transcription activators and the transcriptional machinery. To precisely describe the very early genomic response developed by yeast to accommodate a chemical stress, we performed time course analyses of the modifications of the yeast gen...

journal_title：Molecular and cellular biology

authors： Lucau-Danila A,Lelandais G,Kozovska Z,Tanty V,Delaveau T,Devaux F,Jacq C

更新日期：2005-03-01 00:00:00

abstract：:Nucleosomes were reconstituted in vitro with several singly end-labeled restriction fragments derived from a cloned somatic-type 5S RNA gene of Xenopus laevis and purified nucleosome core particles from Xenopus cultured cells or chicken erythrocytes. Nucleosome locations were determined by digestion of the reconstitut...

journal_title：Molecular and cellular biology

authors： Gottesfeld JM

更新日期：1987-05-01 00:00:00

abstract：:We isolated the gene for citrate synthase (citrate oxaloacetate lyase; EC 4.1.3.7) from Saccharomyces cerevisiae and ablated it by inserting the yeast LEU2 gene within its reading frame. This revealed a second, nonmitochondrial citrate synthase. Like the mitochondrial enzyme, this enzyme was sensitive to glucose repre...

journal_title：Molecular and cellular biology

authors： Rickey TM,Lewin AS

更新日期：1986-02-01 00:00:00

abstract：:The Drosophila melanogaster GAGA factor (encoded by the Trithorax-like [Trl] gene) is required for correct chromatin architecture at diverse chromosomal sites. The Trl gene encodes two alternatively spliced isoforms of the GAGA factor (GAGA-519 and GAGA-581) that are identical except for the length and sequence of the...

journal_title：Molecular and cellular biology

authors： Greenberg AJ,Schedl P

更新日期：2001-12-01 00:00:00

abstract：:Peroxisomal matrix proteins are synthesized on cytosolic ribosomes and transported to the organelle by shuttling receptors. Matrix proteins containing a type 1 signal are carried to the peroxisome by PEX5, whereas those harboring a type 2 signal are transported by a PEX5-PEX7 complex. The pathway followed by PEX5 duri...

journal_title：Molecular and cellular biology

authors： Rodrigues TA,Alencastre IS,Francisco T,Brites P,Fransen M,Grou CP,Azevedo JE

更新日期：2014-08-01 00:00:00

abstract：:We examined the relationship between pre-mRNA splicing and the nuclear matrix by using an in vivo system that we have developed. Plasmids containing the inducible herpesvirus tk gene promoter linked to an intron-containing segment of the rabbit beta-globin gene were transfected into HeLa cells, and then the promoter w...

journal_title：Molecular and cellular biology

authors： Zeitlin S,Parent A,Silverstein S,Efstratiadis A

更新日期：1987-01-01 00:00:00

abstract：:Matrix metalloproteinases (MMPs) exert both pro- and antiangiogenic functions by the release of cytokines or proteolytically generated angiogenic inhibitors from extracellular matrix and basement membrane remodeling. In the Mmp2-/- mouse neovascularization is greatly reduced, but the mechanistic aspects of this remain...

journal_title：Molecular and cellular biology

authors： Dean RA,Butler GS,Hamma-Kourbali Y,Delbé J,Brigstock DR,Courty J,Overall CM

更新日期：2007-12-01 00:00:00

abstract：:Mutations in the spin gene are characterized by an extraordinarily strong rejection behavior of female flies in response to male courtship. They are also accompanied by decreases in the viability, adult life span, and oviposition rate of the flies. In spin mutants, some oocytes and adult neural cells undergo degenerat...

journal_title：Molecular and cellular biology

authors： Nakano Y,Fujitani K,Kurihara J,Ragan J,Usui-Aoki K,Shimoda L,Lukacsovich T,Suzuki K,Sezaki M,Sano Y,Ueda R,Awano W,Kaneda M,Umeda M,Yamamoto D

更新日期：2001-06-01 00:00:00

abstract：:Sequences of DNA that hybridize on Southern blots with cloned intracisternal A-particle (IAP) sequences have been examined in genomic DNAs of neonatal mice, livers of adult mice (3, 6, 12, 18, 24, and 26 months old), and the solid myeloma tumor MOPC-315. The isoschizomers HpaII (CCGG or mCCGG) and MspI (CCGG or CmCGG)...

journal_title：Molecular and cellular biology

authors： Mays-Hoopes LL,Brown A,Huang RC

更新日期：1983-08-01 00:00:00

abstract：:Elastin, an extracellular component of arteries, lung, and skin, is produced during fetal and neonatal growth. We reported previously that the cessation of elastin production is controlled by a posttranscriptional mechanism. Although tropoelastin pre-mRNA is transcribed at the same rate in neonates and adults, marked ...

journal_title：Molecular and cellular biology

authors： Zhang M,Pierce RA,Wachi H,Mecham RP,Parks WC

更新日期：1999-11-01 00:00:00

abstract：:3'-end processing of nucleus-encoded mRNAs includes the addition of a poly(A) tail that is important for translation initiation. Since the vast majority of chloroplast mRNAs acquire their 3' termini by processing yet are not polyadenylated, we asked whether 3' end maturation plays a role in chloroplast translation. A ...

journal_title：Molecular and cellular biology

authors： Rott R,Levy H,Drager RG,Stern DB,Schuster G

更新日期：1998-08-01 00:00:00

abstract：:Approximately 30% of human tumors examined for mutations in polymerase beta (pol beta) appear to express pol beta variant proteins (D. Starcevic, S. Dalal, and J. B. Sweasy, Cell Cycle 3:998-1001, 2004). Many of these variants result from a single amino acid substitution. We have previously shown that the K289M and I2...

journal_title：Molecular and cellular biology

authors： Lang T,Dalal S,Chikova A,DiMaio D,Sweasy JB

更新日期：2007-08-01 00:00:00

abstract：:S1 mapping of the termination region in the ribosomal DNA from a thermophilic fungus, Thermomyces lanuginosus, revealed three distinct termini corresponding to the mature 25S rRNA, a precursor that is 19 nucleotides longer and corresponds to the 37S precursor in yeast cells, and a putative termination site at +96 that...

journal_title：Molecular and cellular biology

authors： Walker K,Wong WM,Nazar RN

更新日期：1990-01-01 00:00:00

abstract：:Microtubules in yeast are essential components of the mitotic and meiotic spindles and are essential for nuclear movement during cell division and mating. The relative importance in these processes of the two divergent alpha-tubulin genes of the budding yeast Saccharomyces cerevisiae, TUB1 and TUB3, was examined throu...

journal_title：Molecular and cellular biology

authors： Schatz PJ,Solomon F,Botstein D

更新日期：1986-11-01 00:00:00

abstract：:The activities of the related Abl and Arg nonreceptor tyrosine kinases are kept under tight control in cells, but exposure to several different stimuli results in a two- to fivefold stimulation of kinase activity. Following the breakdown of inhibitory intramolecular interactions, Abl activation requires phosphorylatio...

journal_title：Molecular and cellular biology

authors： Tanis KQ,Veach D,Duewel HS,Bornmann WG,Koleske AJ

更新日期：2003-06-01 00:00:00

abstract：:Two-dimensional protein gels were used to systematically assess changes in gene expression in Friend erythroleukemia cells after exposure to inducers of differentiation. A rapid decrease in expression of the stress protein HSP70 was observed after exposure to inducers. The kinetics of this change suggest that it may b...

journal_title：Molecular and cellular biology

authors： Hensold JO,Housman DE

更新日期：1988-05-01 00:00:00

abstract：:SATB1 is expressed primarily in thymocytes and orchestrates temporal and spatial expression of a large number of genes in the T-cell lineage. SATB1 binds to the bases of chromatin loop domains in vivo, recognizing a special DNA context with strong base-unpairing propensity. The majority of thymocytes are eliminated by...

journal_title：Molecular and cellular biology

authors： Galande S,Dickinson LA,Mian IS,Sikorska M,Kohwi-Shigematsu T

更新日期：2001-08-01 00:00:00