Abstract:
:A novel small nuclear ribonucleoprotein (snRNP) complex containing both U11 and U12 RNAs has been identified in HeLa cell extracts. This U11/U12 snRNP complex can be visualized on glycerol gradients, on native polyacrylamide gels, and by selection with antisense 2'-O-methyl oligoribonucleotides. RNase H-mediated degradation of the U12 snRNA confirmed a direct interaction between the U11 and U12 snRNPs. This snRNP complex is the first to be identified involving low-abundance snRNPs. Selection of the U11/U12 snRNP complex is sensitive to high salt, suggestive of a protein-mediated interaction. Secondary structure analyses revealed several regions of the U11 snRNP accessible for interaction with other RNAs or proteins but no detectable difference between the accessibility of these regions in the U11 monoparticle compared with the U11/U12 snRNP complex. There are also several accessible single-stranded regions in the U12 snRNP, and oligonucleotide-directed RNase H digestion identified nucleotides 28 to 36 of U12 as containing sequences required for the U11/U12 interaction. Both the U12 snRNP and the U11/U12 snRNP complex can be disrupted without altering the cleavage/polyadenylation activity of a nuclear extract.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Wassarman KM,Steitz JAdoi
10.1128/mcb.12.3.1276subject
Has Abstractpub_date
1992-03-01 00:00:00pages
1276-85issue
3eissn
0270-7306issn
1098-5549journal_volume
12pub_type
杂志文章abstract::The NF-kappa B/Rel family of transcription factors participates in the control of a wide array of genes, including genes involved in embryonic development and regulation of immune, inflammation, and stress responses. In most cells, inhibitory I kappa B proteins sequester NF-kappa B/Rel in the cytoplasm. Cellular stimu...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.23.4.1418-1427.2003
更新日期:2003-02-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.01713-06
更新日期:2007-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1997-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1993-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1997-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-01-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:1992-08-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.00553-09
更新日期:2009-09-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.16.3.1157
更新日期:1996-03-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.22.5.1438-1445.2002
更新日期:2002-03-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.16.1.309
更新日期:1996-01-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.00206-17
更新日期:2017-09-26 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.17.8.4526
更新日期:1997-08-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章,收录出版
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更新日期:2003-06-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.24.6.2536-2545.2004
更新日期:2004-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1986-11-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.13.10.6201
更新日期:1993-10-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:2009-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1998-06-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.6.4.1315
更新日期:1986-04-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.16.6.3085
更新日期:1996-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1993-02-01 00:00:00
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更新日期:2016-05-02 00:00:00
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pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.7.12.4204
更新日期:1987-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/mcb.23.5.1832-1842.2003
更新日期:2003-03-01 00:00:00