Analysis of Small Critical Regions of Swi1 Conferring Prion Formation, Maintenance, and Transmission.

abstract：:Regulation of the activity of N-methyl-d-aspartate receptors (NMDARs) at glutamatergic synapses is essential for certain forms of synaptic plasticity underlying learning and memory and is also associated with neurotoxicity and neurodegenerative diseases. In this report, we investigate the role of Src-like adaptor prot...

journal_title：Molecular and cellular biology

authors： Semerdjieva S,Abdul-Razak HH,Salim SS,Yáñez-Muñoz RJ,Chen PE,Tarabykin V,Alifragis P

更新日期：2013-04-01 00:00:00

abstract：:The RGT1 gene of Saccharomyces cerevisiae plays a central role in the glucose-induced expression of hexose transporter (HXT) genes. Genetic evidence suggests that it encodes a repressor of the HXT genes whose function is inhibited by glucose. Here, we report the isolation of RGT1 and demonstrate that it encodes a bifu...

journal_title：Molecular and cellular biology

authors： Ozcan S,Leong T,Johnston M

更新日期：1996-11-01 00:00:00

abstract：:As shown by Southern blot analysis, the metallothionein-1 (MT-1) genes in rats comprise a multigene family. We present the sequence of the MT-1 structural gene and compare its features with other metallothionein genes. Three MT-1 pseudogenes which we sequenced apparently arose by reverse transcription of processed mRN...

journal_title：Molecular and cellular biology

authors： Andersen RD,Birren BW,Taplitz SJ,Herschman HR

更新日期：1986-01-01 00:00:00

abstract：:Woodchucks infected with woodchuck hepatitis virus (WHV) and ground squirrels infected with ground squirrel hepatitis virus (GSHV) both develop hepatocellular carcinoma (HCC), but WHV-associated tumors arise more frequently and much earlier in life. These differences are preserved when the oncogenic potentials of the ...

journal_title：Molecular and cellular biology

authors： Hansen LJ,Tennant BC,Seeger C,Ganem D

更新日期：1993-01-01 00:00:00

abstract：:During cerebellum development, Sonic hedgehog (Shh)-induced proliferation of cerebellar granular neuronal precursors (CGNPs) is potently inhibited by bone morphogenetic proteins (BMPs). We have previously reported the upregulation of TIEG-1 and Mash1, two antimitotic factors that modulate MYCN transcription and N-Myc ...

journal_title：Molecular and cellular biology

authors： Berenguer J,Herrera A,Vuolo L,Torroba B,Llorens F,Sumoy L,Pons S

更新日期：2013-07-01 00:00:00

abstract：:Tenomodulin (Tnmd) is a member of a new family of type II transmembrane glycoproteins. It is predominantly expressed in tendons, ligaments, and eyes, whereas the only other family member, chondromodulin I (ChM-I), is highly expressed in cartilage and at lower levels in the eye and thymus. The C-terminal extracellular ...

journal_title：Molecular and cellular biology

authors： Docheva D,Hunziker EB,Fässler R,Brandau O

更新日期：2005-01-01 00:00:00

abstract：:Recombination enhancer (RE) is essential for regulating donor preference during yeast mating type switching. In this study, by using minichromosome affinity purification (MAP) and mass spectrometry, we found that yeast Ku80p is associated with RE in MATa cells. Chromatin immunoprecipitation assays confirmed its occupa...

journal_title：Molecular and cellular biology

authors： Ruan C,Workman JL,Simpson RT

更新日期：2005-10-01 00:00:00

abstract：:Nucleosomes were reconstituted in vitro with several singly end-labeled restriction fragments derived from a cloned somatic-type 5S RNA gene of Xenopus laevis and purified nucleosome core particles from Xenopus cultured cells or chicken erythrocytes. Nucleosome locations were determined by digestion of the reconstitut...

journal_title：Molecular and cellular biology

authors： Gottesfeld JM

更新日期：1987-05-01 00:00:00

abstract：:We report in vivo analysis of histone and RNA polymerase II (pol II) occupancy at the 601 element, which functions as a strong in vitro nucleosome-positioning element and transcriptional pause site. Surprisingly, nucleosomes were not strongly positioned over the 601 element inserted either within a yeast chromosomal o...

journal_title：Molecular and cellular biology

authors： Perales R,Zhang L,Bentley D

更新日期：2011-08-01 00:00:00

abstract：:Plasmids containing the hormone regulatory element of mouse mammary tumor virus linked to the thymidine kinase promoter of herpes simplex virus and the reporter gene chloramphenicol acetyltransferase of Escherichia coli respond to glucocorticoids and progestins when transfected into appropriate cells. In the human mam...

journal_title：Molecular and cellular biology

authors： Piña B,Haché RJ,Arnemann J,Chalepakis G,Slater EP,Beato M

更新日期：1990-02-01 00:00:00

abstract：:We investigated the possible interactions between pp39mos and p34cdc2 kinase in NIH 3T3 cells transformed by c-mosxe. pp39mos is coprecipitated with p34cdc2 when using either anti-PSTAIR antibody or p13suc1-Sepharose beads. Likewise, p34cdc2 is coprecipitated with pp39mos when using anti-mos antibody. However, pp39mos...

journal_title：Molecular and cellular biology

authors： Zhou R,Daar I,Ferris DK,White G,Paules RS,Vande Woude G

更新日期：1992-08-01 00:00:00

abstract：:The lck proto-oncogene encodes a lymphocyte-specific member of the src family of protein tyrosine kinases. Here we demonstrate that pp56lck is phosphorylated in vivo at a carboxy-terminal tyrosine residue (Tyr-505) analogous to Tyr-527 of pp60c-src. Substitution of phenylalanine for tyrosine at this position resulted ...

journal_title：Molecular and cellular biology

authors： Marth JD,Cooper JA,King CS,Ziegler SF,Tinker DA,Overell RW,Krebs EG,Perlmutter RM

更新日期：1988-02-01 00:00:00

abstract：:RSC is an essential chromatin remodeling complex in Saccharomyces cerevisiae that performs central roles in transcriptional regulation and cell cycle progression. Here we identify Htl1 as a novel factor that associates with the RSC complex both physically and functionally. We isolated HTL1 through a genetic screen for...

journal_title：Molecular and cellular biology

authors： Romeo MJ,Angus-Hill ML,Sobering AK,Kamada Y,Cairns BR,Levin DE

更新日期：2002-12-01 00:00:00

abstract：:Transfection of a functional cloned p53 gene into an L12 p53 nonproducer cell line efficiently reconstituted p53 expression. The p53 protein synthesized in these clones was indistinguishable from that occurring naturally in tumor cells. When a p53 cDNA clone was used instead, we observed that the L12-derived clones ex...

journal_title：Molecular and cellular biology

authors： Arai N,Nomura D,Yokota K,Wolf D,Brill E,Shohat O,Rotter V

更新日期：1986-09-01 00:00:00

abstract：:Individual steps in the processing of pre-mRNA, including 5'-end cap formation, splicing, and 3'-end processing (cleavage and polyadenylation) are highly integrated and can influence one another. In addition, prior splicing can influence downstream steps in gene expression, including export of mRNA from the nucleus. H...

journal_title：Molecular and cellular biology

authors： McCracken S,Lambermon M,Blencowe BJ

更新日期：2002-01-01 00:00:00

abstract：:The transcription and DNA repair factor TFIIH is composed of 10 subunits. Mutations in the XPB, XPD, and p8 subunits are genetically linked to human diseases, including cancer. However, no reports of mutations in other TFIIH subunits have been reported in higher eukaryotes. Here, we analyze at genetic, molecular, and ...

journal_title：Molecular and cellular biology

authors： Fregoso M,Lainé JP,Aguilar-Fuentes J,Mocquet V,Reynaud E,Coin F,Egly JM,Zurita M

更新日期：2007-05-01 00:00:00

abstract：:Several factors that mediate activation by nuclear receptors also modify the chemical and structural composition of chromatin. Prominent in this diverse group is the steroid receptor coactivator 1 (SRC-1) family, which interact with agonist-bound nuclear receptors, thereby coupling them to multifunctional transcriptio...

journal_title：Molecular and cellular biology

authors： DiRenzo J,Shang Y,Phelan M,Sif S,Myers M,Kingston R,Brown M

更新日期：2000-10-01 00:00:00

abstract：:Thyroid hormone (T(3)) activates nuclear receptor transcription factors, encoded by the TRalpha (NR1A1) and TRbeta (NR1A2) genes, to regulate target gene expression. Several TR isoforms exist, and studies of null mice have identified some unique functions for individual TR variants, although considerable redundancy oc...

journal_title：Molecular and cellular biology

authors： Williams GR

更新日期：2000-11-01 00:00:00

abstract：:The RNA polymerase III transcription initiation factor TFIIIB contains the TATA-box-binding protein (TBP) and polymerase III-specific TBP-associated factors (TAFs). Previous studies have shown that DNA oligonucleotides containing the consensus TATA-box sequence inhibit polymerase III transcription, implying that the D...

journal_title：Molecular and cellular biology

authors： McBryant SJ,Meier E,Leresche A,Sharp SJ,Wolf VJ,Gottesfeld JM

更新日期：1996-09-01 00:00:00

abstract：:Fusions between the TRM1 gene of Saccharomyces cerevisiae and COXIV or DHFR were made to examine the mitochondrial targeting signals of N2,N2-dimethylguanosine-specific tRNA methyltransferase [tRNA (m2(2)G)dimethyltransferase]. This enzyme is responsible for the modification of both mitochondrial and cytoplasmic tRNAs...

journal_title：Molecular and cellular biology

authors： Ellis SR,Hopper AK,Martin NC

更新日期：1989-04-01 00:00:00

abstract：:The adenovirus type 5 early region 1A (E1A) gene was introduced into neu-transformed B104-1-1 cells. Cells that expressed E1A possessed reduced transforming activity in vitro and reduced tumorigenicity in nude mice. These results demonstrate that the E1A gene products can act negatively to suppress the transformed phe...

journal_title：Molecular and cellular biology

authors： Yu DH,Scorsone K,Hung MC

更新日期：1991-03-01 00:00:00

abstract：:A transcriptional enhancer has been mapped to a region 5.5 kilobases 3' of the C beta 2 gene in the human T-cell receptor (TCR) beta-chain locus. Transient transfections allowed localization of enhancer activity to a 480-base-pair HincII-XbaI restriction enzyme fragment. The TCR beta enhancer was active on both the mi...

journal_title：Molecular and cellular biology

authors： Gottschalk LR,Leiden JM

更新日期：1990-10-01 00:00:00

abstract：:Minisatellite DNA is repetitive DNA with a repeat unit length from 15 to 100 bp. While stable during mitosis, it destabilizes during meiosis, altering both in length and in sequence composition. The basis for this instability is unknown. To investigate the factors controlling minisatellite stability, a minisatellite s...

journal_title：Molecular and cellular biology

authors： Jauert PA,Edmiston SN,Conway K,Kirkpatrick DT

更新日期：2002-02-01 00:00:00

abstract：:Sphingomyelin is a major lipid in the bilayer of subcellular membranes of eukaryotic cells. Different sphingomyelinases catalyze the initial step in the catabolism of sphingomyelin, the hydrolysis to phosphocholine and ceramide. Sphingomyelinases have been postulated to generate ceramide as a lipophilic second messeng...

journal_title：Molecular and cellular biology

authors： Zumbansen M,Stoffel W

更新日期：2002-06-01 00:00:00

abstract：:Growth, patterning, and apoptosis are mutually interactive during development. For example, cells that select an abnormal fate in a developing field are frequently removed by apoptosis. An important issue in this process that needs to be resolved is the mechanism used by cells to discern their correct fate from an abn...

journal_title：Molecular and cellular biology

authors： Adachi-Yamada T,Harumoto T,Sakurai K,Ueda R,Saigo K,O'Connor MB,Nakato H

更新日期：2005-04-01 00:00:00

abstract：:Protein modification by SUMO conjugation has emerged to be an important regulatory event. Recently, the mechanisms through which SUMO elicits its effects on target proteins have been elucidated. One of these is the noncovalent association between SUMO and coregulatory proteins via SUMO interaction motifs (SIMs). We th...

journal_title：Molecular and cellular biology

authors： Yang SH,Sharrocks AD

更新日期：2010-05-01 00:00:00

abstract：:The carcinoembryonic antigen (CEA) family consists of a large group of evolutionarily divergent glycoproteins. The secreted pregnancy-specific glycoproteins constitute a subgroup within the CEA family. They are predominantly expressed in trophoblast cells throughout placental development and are essential for a positi...

journal_title：Molecular and cellular biology

authors： Finkenzeller D,Fischer B,McLaughlin J,Schrewe H,Ledermann B,Zimmermann W

更新日期：2000-10-01 00:00:00

abstract：:Nucleoporins mediate transport of macromolecules across the nuclear pore complex, yet the function of many individual nucleoporins is largely unresolved. To address this question, we depleted cells of the cytoplasmic nucleoporins Nup214/CAN and Nup358/RanBP2 by RNA interference. Depletion of Nup214 resulted in codeple...

journal_title：Molecular and cellular biology

authors： Hutten S,Kehlenbach RH

更新日期：2006-09-01 00:00:00

abstract：:Chicken heart mesenchymal cells do not proliferate in medium of physiological composition containing plasma (S. Balk, Proc. Natl. Acad. Sci. USA 77:6606-6610, 1980). To understand the molecular events involved in cell quiescence and in the initiation of cell division under physiological conditions, we examined the dif...

journal_title：Molecular and cellular biology

authors： Bédard PA,Balk SD,Gunther HS,Morisi A,Erikson RL

更新日期：1987-04-01 00:00:00

abstract：:Oncoprotein E2a/Pbx1 is produced by the t(1;19) chromosomal translocation of human pre-B acute lymphoblastic leukemia. E2a/Pbx1 blocks differentiation of primary myeloid progenitors but, paradoxically, induces apoptosis in established pre-B-cell lines, and no transforming function of E2a/Pbx1 has been reported in cult...

journal_title：Molecular and cellular biology

authors： Sykes DB,Kamps MP

更新日期：2004-02-01 00:00:00