Abstract:
:We investigated the possible interactions between pp39mos and p34cdc2 kinase in NIH 3T3 cells transformed by c-mosxe. pp39mos is coprecipitated with p34cdc2 when using either anti-PSTAIR antibody or p13suc1-Sepharose beads. Likewise, p34cdc2 is coprecipitated with pp39mos when using anti-mos antibody. However, pp39mos was not present in histone H1 kinase-active p34cdc2 complexes precipitated with anti-p34cdc2 C-terminal peptide antibody even during metaphase of the cell cycle. The molar ratio of p34 to pp39mos in the p13suc1 complex is approximately 2:1. Consistent with the tight association between pp39mos and tubulin, tubulin was also present in equivalent amounts with pp39mos and p34 in the p13suc1 complex. This pp39mos-p34cdc2-tubulin complex may be important in transformation by the mos oncogene.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Zhou R,Daar I,Ferris DK,White G,Paules RS,Vande Woude Gdoi
10.1128/mcb.12.8.3583subject
Has Abstractpub_date
1992-08-01 00:00:00pages
3583-9issue
8eissn
0270-7306issn
1098-5549journal_volume
12pub_type
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