The chromatin structure at the promoter of a glyceraldehyde phosphate dehydrogenase gene from Saccharomyces cerevisiae reflects its functional state.

abstract：:The polypyrimidine tract near the 3' splice site is important for pre-mRNA splicing. Using pseudouridine incorporation and in vivo RNA-guided RNA pseudouridylation, we have identified two important uridines in the polypyrimidine tract of adenovirus pre-mRNA. Conversion of either uridine into pseudouridine leads to a s...

journal_title：Molecular and cellular biology

authors： Chen C,Zhao X,Kierzek R,Yu YT

更新日期：2010-09-01 00:00:00

abstract：:The Drosophila dopa decarboxylase gene, Ddc, is expressed in the hypoderm and in a small number of cells in the central nervous system (CNS). The unique Ddc primary transcript is alternatively spliced in these two tissues. We investigated whether Ddc splicing in the CNS is a general property of the CNS or a unique pro...

journal_title：Molecular and cellular biology

authors： Shen J,Beall CJ,Hirsh J

更新日期：1993-08-01 00:00:00

abstract：:The STE4 gene encodes the beta subunit of a heterotrimeric G protein that is an essential component of the pheromone signal transduction pathway. To identify downstream component(s) of Ste4, we sought pseudo-revertants that restored mating competence to ste4 mutants. The suppressor mot2 was isolated as a recessive mut...

journal_title：Molecular and cellular biology

authors： Irie K,Yamaguchi K,Kawase K,Matsumoto K

更新日期：1994-05-01 00:00:00

abstract：:Preadipocyte factor 1 (Pref-1/Dlk1) inhibits in vitro adipocyte differentiation and has been recently reported to be a paternally expressed imprinted gene at human chromosome 14q32. Studies on human chromosome 14 deletions and maternal uniparental disomy (mUPD) 14 suggest that misexpression of a yet-to-be-identified i...

journal_title：Molecular and cellular biology

authors： Moon YS,Smas CM,Lee K,Villena JA,Kim KH,Yun EJ,Sul HS

更新日期：2002-08-01 00:00:00

abstract：:Enhancer elements modulate promoter activity over vast chromosomal distances, and mechanisms that ensure restrictive interactions between promoters and enhancers are critical for proper control of gene expression. The human beta-globin locus control region (LCR) activates expression of five genes in erythroid cells, i...

journal_title：Molecular and cellular biology

authors： Shimotsuma M,Matsuzaki H,Tanabe O,Campbell AD,Engel JD,Fukamizu A,Tanimoto K

更新日期：2007-08-01 00:00:00

abstract：:We recently reported that Rho kinase is required for sustained ERK signaling and the consequent mid-G(1) phase induction of cyclin D1 in fibroblasts. The results presented here indicate that these Rho kinase effects are mediated by the formation of stress fibers and the consequent clustering of alpha5beta1 integrin. M...

journal_title：Molecular and cellular biology

authors： Roovers K,Assoian RK

更新日期：2003-06-01 00:00:00

abstract：:Wnt signaling is critical to many aspects of development, and aberrant activation of the Wnt signaling pathway can cause cancer. Dishevelled (Dvl) protein plays a central role in this pathway by transducing the signal from the Wnt receptor complex to the beta-catenin destruction complex. Dvl also plays a pivotal role ...

journal_title：Molecular and cellular biology

authors： Cong F,Schweizer L,Varmus H

更新日期：2004-03-01 00:00:00

abstract：:Eukaryotic genomes are divided into independent transcriptional domains by DNA elements known as insulators. The gypsy insulator, a 350-bp element isolated from the Drosophila gypsy retrovirus, contains twelve degenerate binding sites for the Suppressor of Hairy-wing [Su(Hw)] protein. Su(Hw) associates with over 500 n...

journal_title：Molecular and cellular biology

authors： Parnell TJ,Kuhn EJ,Gilmore BL,Helou C,Wold MS,Geyer PK

更新日期：2006-08-01 00:00:00

abstract：:RNase H1 in mammalian cells is present in nuclei and mitochondria. Its absence in mitochondria results in embryonic lethality due to the failure to amplify mitochondrial DNA (mtDNA). Dual localization to mitochondria and nuclei results from differential translation initiation at two in-frame AUGs (M1 and M27) of a sin...

journal_title：Molecular and cellular biology

authors： Suzuki Y,Holmes JB,Cerritelli SM,Sakhuja K,Minczuk M,Holt IJ,Crouch RJ

更新日期：2010-11-01 00:00:00

abstract：:Polycomb group proteins mediate heritable transcriptional silencing and function through multiprotein complexes that methylate and ubiquitinate histones. The 600-kDa E(Z)/ESC complex, also known as Polycomb repressive complex 2 (PRC2), specifically methylates histone H3 lysine 27 (H3 K27) through the intrinsic histone...

journal_title：Molecular and cellular biology

authors： Tie F,Stratton CA,Kurzhals RL,Harte PJ

更新日期：2007-03-01 00:00:00

abstract：:The simian virus 40 72-base-pair repeats substituted for the polyomavirus enhancer, allowing replication and transcription in mouse 3T6 but not monkey CV-1 cells. A polyomavirus genome containing the entire simian virus 40 control region replicated at low levels in 3T6 and CV-1 cells; however, transcripts were detecte...

journal_title：Molecular and cellular biology

authors： Campbell BA,Villarreal LP

更新日期：1985-06-01 00:00:00

abstract：:The assembly of alpha-ketoglutarate dehydrogenase complex (KGDC) has been studied in wild-type Saccharomyces cerevisiae and in respiratory-deficient strains (pet) with mutations in KGD1 and KGD2, the structural genes for alpha-ketoglutarate dehydrogenase (KE1) and dihydrolipoyl transsuccinylase (KE2) components, respe...

journal_title：Molecular and cellular biology

authors： Repetto B,Tzagoloff A

更新日期：1991-08-01 00:00:00

abstract：:Bone-specific transcription of the osteocalcin (OC) gene is regulated principally by the Runx2 transcription factor and is further stimulated in response to 1alpha,25-dihydroxyvitamin D3 via its specific receptor (VDR). The rat OC gene promoter contains three recognition sites for Runx2 (sites A, B, and C). Mutation o...

journal_title：Molecular and cellular biology

authors： Paredes R,Arriagada G,Cruzat F,Villagra A,Olate J,Zaidi K,van Wijnen A,Lian JB,Stein GS,Stein JL,Montecino M

更新日期：2004-10-01 00:00:00

abstract：:Phorbol-12-myristate-13-acetate (PMA), a potent tumor promoter, was shown to have opposite effects on the cellular morphology and steady-state levels of beta-actin mRNA in embryonic chicken muscle fibroblasts and sternal chondrocytes. When fibroblasts were treated with PMA, they formed foci of densely packed cells, ce...

journal_title：Molecular and cellular biology

authors： Gerstenfeld LC,Finer MH,Boedtker H

更新日期：1985-06-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the TPI gene product, triosephosphate isomerase, makes up about 2% of the soluble cellular protein. Using in vitro and in vivo footprinting techniques, we have identified four binding sites for three factors in the 5' noncoding region of TPI: a REB1-binding site located at positions -401 t...

journal_title：Molecular and cellular biology

authors： Scott EW,Baker HV

更新日期：1993-01-01 00:00:00

abstract：:The extracellular signal-regulated kinase 1 and 2 (ERK1/2) mitogen-activated protein (MAP) kinase signaling pathway plays an important role in the proliferative response of mammalian cells to mitogens. However, the individual contribution of the isoforms ERK1 and ERK2 to cell proliferation control is unclear. The two ...

journal_title：Molecular and cellular biology

authors： Voisin L,Saba-El-Leil MK,Julien C,Frémin C,Meloche S

更新日期：2010-06-01 00:00:00

abstract：:The ability of the zeste moiety of beta-galactosidase-zeste fusion proteins synthesized in Escherichia coli to bind specific DNA sequences was examined. Such fusion proteins recognize a region of the white locus upstream of the start of transcription; this region has previously been shown to be required for genetic in...

journal_title：Molecular and cellular biology

authors： Mansukhani A,Crickmore A,Sherwood PW,Goldberg ML

更新日期：1988-02-01 00:00:00

abstract：:A major goal of cancer research is the identification of tumor-specific vulnerabilities that can be exploited for the development of therapies that are selectively toxic to the tumor. We show here that the transcriptional coactivators peroxisome proliferator-activated receptor gamma coactivator 1β (PGC1β) and estrogen...

journal_title：Molecular and cellular biology

authors： Fisher KW,Das B,Kim HS,Clymer BK,Gehring D,Smith DR,Costanzo-Garvey DL,Fernandez MR,Brattain MG,Kelly DL,MacMillan J,White MA,Lewis RE

更新日期：2015-11-01 00:00:00

abstract：:Cell lines established from the lepidopteran insect Spodoptera frugiperda (fall armyworm; Sf9) are used routinely as hosts for the expression of foreign proteins by recombinant baculovirus vectors. We have examined the pathway of protein glycosylation and secretion in these cells, using human tissue plasminogen activa...

journal_title：Molecular and cellular biology

authors： Jarvis DL,Summers MD

更新日期：1989-01-01 00:00:00

abstract：:Little is known about the regions of RNA polymerase II (RNAPII) that are involved in the process of transcript elongation and interaction with elongation factors. One elongation factor, TFIIS, stimulates transcript elongation by binding to RNAPII and facilitating its passage through intrinsic pausing sites in vitro. I...

journal_title：Molecular and cellular biology

authors： Archambault J,Lacroute F,Ruet A,Friesen JD

更新日期：1992-09-01 00:00:00

abstract：:Transforming growth factor beta (TGF-beta) plays important roles in the regulation of proliferation, differentiation, apoptosis, and carcinogenesis. To identify genes responsible for maintaining the phenotype induced by TGF-beta, we performed a retrovirus-mediated gene trap screening designed to isolate TGF-beta-respo...

journal_title：Molecular and cellular biology

authors： Akiyama N,Matsuo Y,Sai H,Noda M,Kizaka-Kondoh S

更新日期：2000-05-01 00:00:00

abstract：:Histone acetylation provides a switch between transcriptionally repressive and permissive chromatin. By regulating the chromatin structure at specific promoters, histone acetyltransferases (HATs) carry out important functions during differentiation and development of higher eukaryotes. HAT complexes are present in org...

journal_title：Molecular and cellular biology

authors： Guelman S,Suganuma T,Florens L,Weake V,Swanson SK,Washburn MP,Abmayr SM,Workman JL

更新日期：2006-10-01 00:00:00

abstract：:The four receptor tyrosine kinases of the ErbB family play essential roles in several physiological processes and have also been implicated in tumor generation and/or progression. Activation of ErbB1/EGFR is mainly triggered by epidermal growth factor (EGF) and other related ligands, while activation of ErbB2, ErbB3, ...

journal_title：Molecular and cellular biology

authors： Esparís-Ogando A,Díaz-Rodríguez E,Montero JC,Yuste L,Crespo P,Pandiella A

更新日期：2002-01-01 00:00:00

abstract：:The inducer of short transcripts, or IST, is an unusual transcriptional element located downstream of the human immunodeficiency virus type 1 (HIV-1) promoter. IST activates HIV-1 transcription, but the resulting RNAs are short and end at approximately position +59. IST, therefore, appears to promote the formation of ...

journal_title：Molecular and cellular biology

authors： Sheldon M,Ratnasabapathy R,Hernandez N

更新日期：1993-02-01 00:00:00

abstract：:The mouse myosin light-chain 1A (MLC1A) gene, expressed in the atria of the adult heart, is one of the first muscle genes to be activated when skeletal as well as cardiac muscles form in the embryo. It is also transcribed in skeletal muscle cell lines at the onset of differentiation. Transient transfection assays of m...

journal_title：Molecular and cellular biology

authors： Catala F,Wanner R,Barton P,Cohen A,Wright W,Buckingham M

更新日期：1995-08-01 00:00:00

abstract：:Cell polarity plays a critical role in the development of all metazoans; however, the mechanisms of cell polarity and the specific role of cell polarity pathways in mammalian organisms are still poorly understood. Lethal giant larvae (Lgl) is an apical-basal polarity gene identified in Drosophila, where it functions a...

journal_title：Molecular and cellular biology

authors： Sripathy S,Lee M,Vasioukhin V

更新日期：2011-07-01 00:00:00

abstract：:Mutations in the gene encoding methyl-CpG-binding protein 2 (MeCP2) lead to disrupted neuronal function and can cause the neurodevelopmental disorder Rett syndrome. MeCP2 is a transcriptional regulator that binds to methylated DNA and is most abundant in neuronal nuclei. The mechanisms by which MeCP2 regulates gene ex...

journal_title：Molecular and cellular biology

authors： Gonzales ML,Adams S,Dunaway KW,LaSalle JM

更新日期：2012-07-01 00:00:00

abstract：:The Ran binding protein RanBP1 is localized to the cytosol of interphase cells. A leucine-rich nuclear export signal (NES) near the C terminus of RanBP1 is essential to maintain this distribution. We now show that RanBP1 accumulates in nuclei of cells treated with the export inhibitor, leptomycin B, and collapse of th...

journal_title：Molecular and cellular biology

authors： Plafker K,Macara IG

更新日期：2000-05-01 00:00:00

abstract：:Study of the mechanism(s) of genomic instability induced by the c-myc proto-oncogene has the potential to shed new light on its well-known oncogenic activity. However, an underlying mechanism(s) for this phenotype is largely unknown. In the present study, we investigated the effects of c-Myc overexpression on the DNA ...

journal_title：Molecular and cellular biology

authors： Sheen JH,Dickson RB

更新日期：2002-03-01 00:00:00

abstract：:The histone H1(0)-encoding gene is expressed in vertebrates in differentiating cells during the arrest of proliferation. In the H1(0) promoter, a specific regulatory element, which we named the H4 box, exhibits features which implicate a role in mediating H1(0) gene expression in response to both differentiation and c...

journal_title：Molecular and cellular biology

authors： Lemercier C,Duncliffe K,Boibessot I,Zhang H,Verdel A,Angelov D,Khochbin S

更新日期：2000-09-01 00:00:00