Genetic demonstration of a redundant role of extracellular signal-regulated kinase 1 (ERK1) and ERK2 mitogen-activated protein kinases in promoting fibroblast proliferation.

abstract：:Microinjection of p21Ha-ras proteins into Xenopus laevis oocytes induces a rapid increase of 1,2-diacylglycerol (DAG) levels. The observed alterations in DAG levels were consistent with the ability of the protein to induce maturation, measured by germinal vesicle breakdown (GVBD). Both the increase in DAG levels and G...

journal_title：Molecular and cellular biology

authors： Lacal JC

更新日期：1990-01-01 00:00:00

abstract：:In an effort to identify aberrantly expressed genes in v-rel-induced tumors, monoclonal antibodies were developed that reacted selectively with avian B-cell tumors. One antibody, HY78, immunoprecipitated a 120-kDa glycoprotein (p120) from cells that express v-rel. N-terminal amino acid sequencing of p120 identified a ...

journal_title：Molecular and cellular biology

authors： Zhang G,Slaughter C,Humphries EH

更新日期：1995-03-01 00:00:00

abstract：:Selenoprotein mRNAs are potential targets for degradation via nonsense-mediated decay due to the presence of in-frame UGA codons that can be decoded as either selenocysteine or termination codons. When UGA decoding is inefficient, as occurs when selenium is limiting, termination occurs at these positions. Based on the...

journal_title：Molecular and cellular biology

authors： Squires JE,Stoytchev I,Forry EP,Berry MJ

更新日期：2007-11-01 00:00:00

abstract：:Oncostatin M is a polypeptide cytokine, produced by normal and malignant hematopoietic cells, that has several in vitro activities, including the ability to inhibit growth of cultured carcinoma cells. Here we present a structural and functional comparison of two oncostatin M-related proteins (Mr 36,000 and 32,000) sec...

journal_title：Molecular and cellular biology

authors： Linsley PS,Kallestad J,Ochs V,Neubauer M

更新日期：1990-05-01 00:00:00

abstract：:To investigate in vivo roles of a murine hypothalamic homeobox gene, Bsx, we generated and analyzed two mutant alleles, Bsx(DeltaHD) and Bsx(lacZ). Bsx(DeltaHD) lacks the homeodomain, and Bsx(lacZ) is an insertion of a lacZ reporter gene. Bsx-lacZ expression was detected in the hypothalamus and pineal gland and reiter...

journal_title：Molecular and cellular biology

authors： McArthur T,Ohtoshi A

更新日期：2007-07-01 00:00:00

abstract：:The Ras-RAF-mitogen-activated protein kinase (Ras-RAF-MAPK) pathway is overactive in many cancers and in some developmental disorders. In one of those disorders, namely, Noonan syndrome, nine activating C-RAF mutations cluster around Ser(259), a regulatory site for inhibition by 14-3-3 proteins. We show that these mut...

journal_title：Molecular and cellular biology

authors： Molzan M,Schumacher B,Ottmann C,Baljuls A,Polzien L,Weyand M,Thiel P,Rose R,Rose M,Kuhenne P,Kaiser M,Rapp UR,Kuhlmann J,Ottmann C

更新日期：2010-10-01 00:00:00

abstract：:We have previously shown by affinity chromatography that RAP30 and RAP74 are the mammalian proteins that have the highest affinity for RNA polymerase II. Here we show that RAP30 binds to RAP74 and that the RAP30-RAP74 complex (RAP30/74) is required for accurate initiation by RNA polymerase II. RAP30/74 is required for...

journal_title：Molecular and cellular biology

authors： Burton ZF,Killeen M,Sopta M,Ortolan LG,Greenblatt J

更新日期：1988-04-01 00:00:00

abstract：:The mammalian signal recognition particle (SRP) is a small cytoplasmic ribonucleoprotein required for the cotranslational targeting of secretory proteins to the endoplasmic reticulum membrane. The heterodimeric protein subunit SRP9/14 was previously shown to be essential for SRP to cause pausing in the elongation of s...

journal_title：Molecular and cellular biology

authors： Strub K,Moss J,Walter P

更新日期：1991-08-01 00:00:00

abstract：:Woodchucks infected with woodchuck hepatitis virus (WHV) and ground squirrels infected with ground squirrel hepatitis virus (GSHV) both develop hepatocellular carcinoma (HCC), but WHV-associated tumors arise more frequently and much earlier in life. These differences are preserved when the oncogenic potentials of the ...

journal_title：Molecular and cellular biology

authors： Hansen LJ,Tennant BC,Seeger C,Ganem D

更新日期：1993-01-01 00:00:00

abstract：:There exist in the Xenopus laevis genome clusters of tandemly repeated DNA sequences, consisting of two types of 393-base-pair repeating unit. Each such cluster contains several units of one of these paired tandem repeats (PTR-1), followed by several units of the other repeat (PTR-2). The number of repeats of each typ...

journal_title：Molecular and cellular biology

authors： Carroll D,Garrett JE,Lam BS

更新日期：1984-02-01 00:00:00

abstract：:Keratin 6 (K6) expression in the epidermis has two components: constitutive expression in the innermost layer of the outer root sheath (ORS) of hair follicles and inducible expression in the interfollicular epidermis in response to stressful stimuli such as wounding. Mice express two K6 isoforms, MK6a and MK6b. To gai...

journal_title：Molecular and cellular biology

authors： Wojcik SM,Bundman DS,Roop DR

更新日期：2000-07-01 00:00:00

abstract：:To understand the mechanisms by which the chromatin-remodeling SWI/SNF complex interacts with DNA and alters nucleosome organization, we have imaged the SWI/SNF complex with both naked DNA and nucleosomal arrays by using energy-filtered microscopy. By making ATP-independent contacts with DNA at multiple sites on its s...

journal_title：Molecular and cellular biology

authors： Bazett-Jones DP,Côté J,Landel CC,Peterson CL,Workman JL

更新日期：1999-02-01 00:00:00

abstract：:The retrotransposon LINE-1 (L1) is a significant source of endogenous mutagenesis in humans. In each individual genome, a few retrotransposition-competent L1s (RC-L1s) can generate new heritable L1 insertions in the early embryo, primordial germ line, and germ cells. L1 retrotransposition can also occur in the neurona...

journal_title：Molecular and cellular biology

authors： Salvador-Palomeque C,Sanchez-Luque FJ,Fortuna PRJ,Ewing AD,Wolvetang EJ,Richardson SR,Faulkner GJ

更新日期：2019-03-19 00:00:00

abstract：:Obesity and type 2 diabetes are characterized by insulin resistance. Mice lacking the protein-tyrosine phosphatase PTP1B in all tissues are hypersensitive to insulin but also have diminished fat stores. Because adiposity affects insulin sensitivity, the extent to which PTP1B directly regulates glucose homeostasis has ...

journal_title：Molecular and cellular biology

authors： Delibegovic M,Bence KK,Mody N,Hong EG,Ko HJ,Kim JK,Kahn BB,Neel BG

更新日期：2007-11-01 00:00:00

abstract：:Small nucleolar RNAs (snoRNAs) orchestrate the modification and cleavage of pre-rRNA and are essential for ribosome biogenesis. Recent data suggest that after nucleoplasmic synthesis, snoRNAs transiently localize to the Cajal body (in plant and animal cells) or the homologous nucleolar body (in budding yeast) for matu...

journal_title：Molecular and cellular biology

authors： Qiu H,Eifert J,Wacheul L,Thiry M,Berger AC,Jakovljevic J,Woolford JL Jr,Corbett AH,Lafontaine DL,Terns RM,Terns MP

更新日期：2008-06-01 00:00:00

abstract：:Mammalian cells were observed to die under conditions in which nutrients were depleted and, simultaneously, macroautophagy was inhibited either genetically (by a small interfering RNA targeting Atg5, Atg6/Beclin 1-1, Atg10, or Atg12) or pharmacologically (by 3-methyladenine, hydroxychloroquine, bafilomycin A1, or mone...

journal_title：Molecular and cellular biology

authors： Boya P,González-Polo RA,Casares N,Perfettini JL,Dessen P,Larochette N,Métivier D,Meley D,Souquere S,Yoshimori T,Pierron G,Codogno P,Kroemer G

更新日期：2005-02-01 00:00:00

abstract：:The serum response factor (SRF) and myocyte enhancer factor 2A (MEF2A) represent two human members of the MADS-box transcription factor family. Each protein has a distinct biological function which is reflected by the distinct specificities of the proteins for coregulatory protein partners and DNA-binding sites. In th...

journal_title：Molecular and cellular biology

authors： West AG,Shore P,Sharrocks AD

更新日期：1997-05-01 00:00:00

abstract：:Cotranslational insertion of type I collagen chains into the lumen of the endoplasmic reticulum (ER) and their subsequent folding into a heterotrimeric helix is a complex process which requires coordinated action of the translation machinery, components of translocons, molecular chaperones, and modifying enzymes. Here...

journal_title：Molecular and cellular biology

authors： Stefanovic B,Stefanovic L,Schnabl B,Bataller R,Brenner DA

更新日期：2004-02-01 00:00:00

abstract：:Cholera toxin (CT) activates expression of two immediate-early response genes (JE and TIS10) in quiescent BALB/c 3T3 cells. Increases in cyclic AMP (cAMP) levels in response to CT are likely responsible for the induction of TIS10 gene expression, since treatment with 8-Br-cAMP and increasing the intracellular levels o...

journal_title：Molecular and cellular biology

authors： Qureshi SA,Alexandropoulos K,Joseph CK,Spangler R,Foster DA

更新日期：1991-01-01 00:00:00

abstract：:Entry into mitosis depends upon activation of the dual-specificity phosphatase Cdc25C, which dephosphorylates and activates the cyclin B-Cdc2 complex. Previous work has shown that the Xenopus polo-like kinase Plx1 can phosphorylate and activate Cdc25C in vitro. In the work presented here, we demonstrate that Plx1 is a...

journal_title：Molecular and cellular biology

authors： Qian YW,Erikson E,Li C,Maller JL

更新日期：1998-07-01 00:00:00

abstract：:A protein that binds specifically to Saccharomyces cerevisiae centromere DNA element I was purified on the basis of a nitrocellulose filter-binding assay. This protein, termed centromere-binding protein 1 (CP1), was heat stable and renaturable from sodium dodecyl sulfate (SDS), and assays of eluates from SDS gels indi...

journal_title：Molecular and cellular biology

authors： Bram RJ,Kornberg RD

更新日期：1987-01-01 00:00:00

abstract：:The complete nucleotide sequence of a genomic clone encoding the mouse skeletal alpha-actin gene has been determined. This single-copy gene codes for a protein identical in primary sequence to the rabbit skeletal alpha-actin. It has a large intron in the 5'-untranslated region 12 nucleotides upstream from the initiato...

journal_title：Molecular and cellular biology

authors： Hu MC,Sharp SB,Davidson N

更新日期：1986-01-01 00:00:00

abstract：:Gonadotropin-releasing hormone (GnRH) interacts with a G protein-coupled receptor and increases the transcription of the glycoprotein hormone alpha-subunit gene. We have explored the possibility that mitogen-activated protein kinase (MAPK) plays a role in mediating GnRH effects on transcription. Activation of the MAPK...

journal_title：Molecular and cellular biology

authors： Roberson MS,Misra-Press A,Laurance ME,Stork PJ,Maurer RA

更新日期：1995-07-01 00:00:00

abstract：:Entry into mitosis is controlled by the cyclin-dependent kinase CDK1 and can be delayed in response to DNA damage. In some systems, such G(2)/M arrest has been shown to reflect the stabilization of inhibitory phosphorylation sites on CDK1. In human cells, full G(2) arrest appears to involve additional mechanisms. We d...

journal_title：Molecular and cellular biology

authors： Badie C,Itzhaki JE,Sullivan MJ,Carpenter AJ,Porter AC

更新日期：2000-04-01 00:00:00

abstract：:Ubiquitously expressed transcription factors play an integral role in establishing and regulating patterns of gene transcription. Common factor 1 (CF1) is a ubiquitously expressed DNA-binding protein previously identified in our laboratory. We show here that CF1 recognizes sites in several diverse transcription elemen...

journal_title：Molecular and cellular biology

authors： Riggs KJ,Merrell KT,Wilson G,Calame K

更新日期：1991-03-01 00:00:00

abstract：:Swi4 and Swi6 form a complex which is required for Start-dependent activation of HO and for high-level expression of G1 cyclin genes CLN1 and CLN2. To identify other regulators of this pathway, we screened for dominant, recessive, conditional, and allele-specific suppressors of swi4 mutants. We isolated 16 recessive s...

journal_title：Molecular and cellular biology

authors： Lycan D,Mikesell G,Bunger M,Breeden L

更新日期：1994-11-01 00:00:00

abstract：:TGF-alpha-PE40 is a hybrid protein composed of transforming growth factor-alpha (TGF-alpha) fused to a 40,000-dalton segment of Pseudomonas exotoxin A (PE40). This hybrid protein possesses the receptor-binding activity of TGF-alpha and the cell-killing properties of PE40. These properties enable TGF-alpha-PE40 to bind...

journal_title：Molecular and cellular biology

authors： Edwards GM,DeFeo-Jones D,Tai JY,Vuocolo GA,Patrick DR,Heimbrook DC,Oliff A

更新日期：1989-07-01 00:00:00

abstract：:The transcription factor LFB1 (HNF1) was initially identified as a regulator of liver-specific gene expression in mammals. It interacts with the promoter element HP1, which is functionally conserved between mammals and amphibians, suggesting that a homologous factor, XLFB1, also exists in Xenopus laevis. To study the ...

journal_title：Molecular and cellular biology

authors： Bartkowski S,Zapp D,Weber H,Eberle G,Zoidl C,Senkel S,Klein-Hitpass L,Ryffel GU

更新日期：1993-01-01 00:00:00

abstract：:Transcriptional activators interact with multisubunit coactivators that modify chromatin structure or recruit the general transcriptional machinery to their target genes. Budding yeast cells respond to amino acid starvation by inducing an activator of amino acid biosynthetic genes, Gcn4p. We conducted a comprehensive ...

journal_title：Molecular and cellular biology

authors： Swanson MJ,Qiu H,Sumibcay L,Krueger A,Kim SJ,Natarajan K,Yoon S,Hinnebusch AG

更新日期：2003-04-01 00:00:00

abstract：:The retinoblastoma susceptibility gene product pRb restricts cellular proliferation by affecting gene expression by all three classes of nuclear RNA polymerases. To elucidate the molecular mechanisms underlying pRb-mediated repression of ribosomal DNA (rDNA) transcription by RNA polymerase I, we have analyzed the effe...

journal_title：Molecular and cellular biology

authors： Voit R,Schäfer K,Grummt I

更新日期：1997-08-01 00:00:00