当前位置： SCI文献检索 > MOLECULAR AND CELLULAR BIOLOGY期刊下所有文献 > Multiple interferon regulatory factor and NF-κB sites cooperate in mediating cell-type- and maturation-specific activation of the human CD83 promoter in dendritic cells.

Multiple interferon regulatory factor and NF-κB sites cooperate in mediating cell-type- and maturation-specific activation of the human CD83 promoter in dendritic cells.

Abstract：

:CD83 is one of the best-known surface markers for fully mature dendritic cells (mature DCs), and its cell-type- and maturation-specific regulation makes the CD83 promoter an interesting tool for the genetic modulation of DCs. To determine the mechanisms regulating this DC- and maturation-specific CD83 expression, chromatin immunoprecipitation (ChIP)-on-chip microarray, biocomputational, reporter, electrophoretic mobility shift assay (EMSA), and ChIP analyses were performed. These studies led to the identification of a ternary transcriptional activation complex composed of an upstream regulatory element, a minimal promoter, and an enhancer, which have not been reported in this arrangement for any other gene so far. Notably, these DNA regions contain a complex framework of interferon regulatory factor (IRF)- and NF-κB transcription factor-binding sites mediating their arrangement. Mutation of any of the IRF-binding sites resulted in a significant loss of promoter activity, whereas overexpression of NF-κB transcription factors clearly enhanced transcription. We identified IRF-1, IRF-2, IRF-5, p50, p65, and cRel to be involved in regulating maturation-specific CD83 expression in DCs. Therefore, the characterization of this promoter complex not only contributes to the knowledge of DC-specific gene regulation but also suggests the involvement of a transcriptional module with binding sites separated into distinct regions in transcriptional activation as well as cell-type- and maturation-specific transcriptional targeting of DCs.

journal_name

Mol Cell Biol

journal_title

Molecular and cellular biology

authors

Stein MF,Lang S,Winkler TH,Deinzer A,Erber S,Nettelbeck DM,Naschberger E,Jochmann R,Stürzl M,Slany RK,Werner T,Steinkasserer A,Knippertz I

doi

10.1128/MCB.01051-12

subject

Has Abstract

pub_date

2013-04-01 00:00:00

pages

1331-44

issue

7

eissn

0270-7306

issn

1098-5549

pii

MCB.01051-12

journal_volume

33

pub_type

杂志文章

相关文献

MOLECULAR AND CELLULAR BIOLOGY文献大全
  • Impaired retinoic acid (RA) signal leads to RARbeta2 epigenetic silencing and RA resistance.

    abstract：:Resistance to the growth-inhibitory action of retinoic acid (RA), the bioactive derivative of vitamin A, is common in human tumors. One form of RA resistance has been associated with silencing and hypermethylation of the retinoic acid receptor beta2 gene (RARbeta2), an RA-regulated tumor suppressor gene. The presence ...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/MCB.25.23.10591-10603.2005

    authors： Ren M,Pozzi S,Bistulfi G,Somenzi G,Rossetti S,Sacchi N

    更新日期：2005-12-01 00:00:00

  • Identification of a peroxisomal ATP carrier required for medium-chain fatty acid beta-oxidation and normal peroxisome proliferation in Saccharomyces cerevisiae.

    abstract：:We have characterized the role of YPR128cp, the orthologue of human PMP34, in fatty acid metabolism and peroxisomal proliferation in Saccharomyces cerevisiae. YPR128cp belongs to the mitochondrial carrier family (MCF) of solute transporters and is localized in the peroxisomal membrane. Disruption of the YPR128c gene r...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/MCB.21.13.4321-4329.2001

    authors： van Roermund CW,Drissen R,van Den Berg M,Ijlst L,Hettema EH,Tabak HF,Waterham HR,Wanders RJ

    更新日期：2001-07-01 00:00:00

  • Transcription factor UAF, expansion and contraction of ribosomal DNA (rDNA) repeats, and RNA polymerase switch in transcription of yeast rDNA.

    abstract：:Strains of the yeast Saccharomyces cerevisiae defective in transcription factor UAF give rise to variants able to grow by transcribing endogenous ribosomal DNA (rDNA) by RNA polymerase II (Pol II). We have demonstrated that the switch to growth using the Pol II system consists of two steps: a mutational alteration in ...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/mcb.19.12.8559

    authors： Oakes M,Siddiqi I,Vu L,Aris J,Nomura M

    更新日期：1999-12-01 00:00:00

  • RAD1, an excision repair gene of Saccharomyces cerevisiae, is also involved in recombination.

    abstract：:The RAD1 gene of Saccharomyces cerevisiae is required for the incision step of excision repair of damaged DNA. In this paper, we report our observations on the effect of the RAD1 gene on genetic recombination. Mitotic intrachromosomal and interchromosomal recombination in RAD+, rad1, rad52, and other rad mutant strain...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/mcb.8.9.3619

    authors： Schiestl RH,Prakash S

    更新日期：1988-09-01 00:00:00

  • O glycosylation of an Sp1-derived peptide blocks known Sp1 protein interactions.

    abstract：:The O-linked N-acetylglucosamine (O-GlcNAc) modification of proteins is dynamic and abundant in the nucleus and cytosol. Several transcription factors, including Sp1, have been shown to contain this modification; however, the functional role of O-GlcNAc in these proteins has not been determined. In this paper we descr...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/mcb.17.11.6472

    authors： Roos MD,Su K,Baker JR,Kudlow JE

    更新日期：1997-11-01 00:00:00

  • Fragments of ATM which have dominant-negative or complementing activity.

    abstract：:The ATM protein has been implicated in pathways controlling cell cycle checkpoints, radiosensitivity, genetic instability, and aging. Expression of ATM fragments containing a leucine zipper motif in a human tumor cell line abrogated the S-phase checkpoint after ionizing irradiation and enhanced radiosensitivity and ch...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/mcb.17.4.2020

    authors： Morgan SE,Lovly C,Pandita TK,Shiloh Y,Kastan MB

    更新日期：1997-04-01 00:00:00

  • A highly specific phosphatase from Saccharomyces cerevisiae implicated in tRNA splicing.

    abstract：:We identified and partially purified a phosphatase from crude extracts of Saccharomyces cerevisiae cells that can catalyze the last step of tRNA splicing in vitro. This phosphatase can remove the 2'-phosphate left over at the splice junction after endonuclease has removed the intron and ligase has joined together the ...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/mcb.10.3.1049

    authors： McCraith SM,Phizicky EM

    更新日期：1990-03-01 00:00:00

  • Partial cleavage of RasGAP by caspases is required for cell survival in mild stress conditions.

    abstract：:Tight control of apoptosis is required for proper development and maintenance of homeostasis in multicellular organisms. Cells can protect themselves from potentially lethal stimuli by expressing antiapoptotic factors, such as inhibitors of apoptosis, FLICE (caspase 8)-inhibitory proteins, and members of the Bcl2 fami...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/MCB.24.23.10425-10436.2004

    authors： Yang JY,Michod D,Walicki J,Murphy BM,Kasibhatla S,Martin SJ,Widmann C

    更新日期：2004-12-01 00:00:00

  • HDAC4, a human histone deacetylase related to yeast HDA1, is a transcriptional corepressor.

    abstract：:Histone acetylation plays an important role in regulating chromatin structure and thus gene expression. Here we describe the functional characterization of HDAC4, a human histone deacetylase whose C-terminal part displays significant sequence similarity to the deacetylase domain of yeast HDA1. HDAC4 is expressed in va...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/mcb.19.11.7816

    authors： Wang AH,Bertos NR,Vezmar M,Pelletier N,Crosato M,Heng HH,Th'ng J,Han J,Yang XJ

    更新日期：1999-11-01 00:00:00

  • E1A-mediated repression of progesterone receptor-dependent transactivation involves inhibition of the assembly of a multisubunit coactivation complex.

    abstract：:The steroid hormone progesterone acts via high-affinity nuclear receptors that interact with specific DNA sequences located near the promoter of the hormone-responsive gene. Recent studies suggested that the hormone-occupied progesterone receptor (PR) mediates gene activation by recruiting a cellular coregulatory fact...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/mcb.20.6.2138-2146.2000

    authors： Xu Y,Klein-Hitpass L,Bagchi MK

    更新日期：2000-03-01 00:00:00

  • Identification of histone mutants that are defective for transcription-coupled nucleosome occupancy.

    abstract：:Our previous studies of Saccharomyces cerevisiae described a gene repression mechanism where the transcription of intergenic noncoding DNA (ncDNA) (SRG1) assembles nucleosomes across the promoter of the adjacent SER3 gene that interfere with the binding of transcription factors. To investigate the role of histones in ...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/MCB.05195-11

    authors： Hainer SJ,Martens JA

    更新日期：2011-09-01 00:00:00

  • Accessibility of ribosomal genes to trimethyl psoralen in nuclei of Physarum polycephalum.

    abstract：:We have probed the accessibility of the genes for rRNA in Physarum polycephalum by using the photoreactive DNA cross-linking agent 4,5',8-trimethyl psoralen. Nuclei isolated from actively growing Physarum were treated with trimethyl psoralen and irradiated with 360-nm light in order to form cross-links. The palindromi...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/mcb.2.3.211

    authors： Judelson HS,Vogt VM

    更新日期：1982-03-01 00:00:00

  • Functions and regulation of circular dorsal ruffles.

    abstract：:Cells construct a number of plasma membrane structures to meet a range of physiological demands. Driven by juxtamembrane actin machinery, these actin-based membrane protrusions are essential for the operation and maintenance of cellular life. They are required for diverse cellular functions, such as directed cell moti...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章,评审

    doi：10.1128/MCB.00551-12

    authors： Hoon JL,Wong WK,Koh CG

    更新日期：2012-11-01 00:00:00

  • Differential activation of the Ras/extracellular-signal-regulated protein kinase pathway is responsible for the biological consequences induced by the Axl receptor tyrosine kinase.

    abstract：:To understand the mechanism of Axl signaling, we have initiated studies to delineate downstream components in interleukin-3-dependent 32D cells by using a chimeric receptor containing the recombinant epidermal growth factor (EGF) receptor extracellular and transmembrane domains and the Axl kinase domain (EAK [for EGF ...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/mcb.16.1.135

    authors： Fridell YW,Jin Y,Quilliam LA,Burchert A,McCloskey P,Spizz G,Varnum B,Der C,Liu ET

    更新日期：1996-01-01 00:00:00

  • Alternative processing of RNA transcribed from NMYC.

    abstract：:NMYC is a gene whose amplification and overexpression have been implicated in the generation of certain human malignancies. Little is known of how the expression of NMYC is normally controlled. We have therefore characterized transcription from the gene and the structure and stability of the resulting mRNAs. Transcrip...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/mcb.7.12.4266

    authors： Stanton LW,Bishop JM

    更新日期：1987-12-01 00:00:00

  • Identification of base and backbone contacts used for DNA sequence recognition and high-affinity binding by LAC9, a transcription activator containing a C6 zinc finger.

    abstract：:The LAC9 protein of Kluyveromyces lactis is a transcriptional regulator of genes in the lactose-galactose regulon. To regulate transcription, LAC9 must bind to 17-bp upstream activator sequences (UASs) located in front of each target gene. LAC9 is homologous to the GAL4 protein of Saccharomyces cerevisiae, and the two...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/mcb.11.4.1777

    authors： Halvorsen YD,Nandabalan K,Dickson RC

    更新日期：1991-04-01 00:00:00

  • Human-mouse interspecies collagen I heterotrimer is functional during embryonic development of Mov13 mutant mouse embryos.

    abstract：:To investigate whether the human pro alpha 1(I) collagen chain could form an in vivo functional interspecies heterotrimer with the mouse pro alpha 2(I) collagen chain, we introduced the human COL1A1 gene into Mov13 mice which have a functional deletion of the endogenous COL1A1 gene. Transgenic mouse strains (HucI and ...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/mcb.10.4.1452

    authors： Wu H,Bateman JF,Schnieke A,Sharpe A,Barker D,Mascara T,Eyre D,Bruns R,Krimpenfort P,Berns A

    更新日期：1990-04-01 00:00:00

  • Tenomodulin is necessary for tenocyte proliferation and tendon maturation.

    abstract：:Tenomodulin (Tnmd) is a member of a new family of type II transmembrane glycoproteins. It is predominantly expressed in tendons, ligaments, and eyes, whereas the only other family member, chondromodulin I (ChM-I), is highly expressed in cartilage and at lower levels in the eye and thymus. The C-terminal extracellular ...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/MCB.25.2.699-705.2005

    authors： Docheva D,Hunziker EB,Fässler R,Brandau O

    更新日期：2005-01-01 00:00:00

  • The nuclear pore complex and the DEAD box protein Rat8p/Dbp5p have nonessential features which appear to facilitate mRNA export following heat shock.

    abstract：:Nuclear pore complexes (NPCs) play an essential role in RNA export. Nucleoporins required for mRNA export in Saccharomyces cerevisiae are found in the Nup84p and Nup82p subcomplexes of the NPC. The Nup82p subcomplex contains Nup82p, Rat7p/Nup159p, Nsp1p, Gle1p/Rss1p, and Rip1p/Nup42p and is found only on the cytoplasm...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/MCB.24.11.4869-4879.2004

    authors： Rollenhagen C,Hodge CA,Cole CN

    更新日期：2004-06-01 00:00:00

  • The amino-terminal C/H1 domain of CREB binding protein mediates zta transcriptional activation of latent Epstein-Barr virus.

    abstract：:Latent Epstein-Barr virus (EBV) is maintained as a nucleosome-covered episome that can be transcriptionally activated by overexpression of the viral immediate-early protein, Zta. We show here that reactivation of latent EBV by Zta can be significantly enhanced by coexpression of the cellular coactivators CREB binding ...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/mcb.19.3.1617

    authors： Zerby D,Chen CJ,Poon E,Lee D,Shiekhattar R,Lieberman PM

    更新日期：1999-03-01 00:00:00

  • Differential regulation of human interferon A gene expression by interferon regulatory factors 3 and 7.

    abstract：:Differential expression of the human interferon A (IFN-A) gene cluster is modulated following paramyxovirus infection by the relative amounts of active interferon regulatory factor 3 (IRF-3) and IRF-7. IRF-3 expression activates predominantly IFN-A1 and IFN-B, while IRF-7 expression induces multiple IFN-A genes. IFN-A...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/MCB.01805-08

    authors： Génin P,Lin R,Hiscott J,Civas A

    更新日期：2009-06-01 00:00:00

  • The protein level of PGC-1α, a key metabolic regulator, is controlled by NADH-NQO1.

    abstract：:PGC-1α is a key transcription coactivator regulating energy metabolism in a tissue-specific manner. PGC-1α expression is tightly regulated, it is a highly labile protein, and it interacts with various proteins--the known attributes of intrinsically disordered proteins (IDPs). In this study, we characterize PGC-1α as a...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/MCB.01672-12

    authors： Adamovich Y,Shlomai A,Tsvetkov P,Umansky KB,Reuven N,Estall JL,Spiegelman BM,Shaul Y

    更新日期：2013-07-01 00:00:00

  • Human Cdc34 and Rad6B ubiquitin-conjugating enzymes target repressors of cyclic AMP-induced transcription for proteolysis.

    abstract：:Ubiquitin-mediated proteolysis controls diverse physiological processes in eukaryotes. However, few in vivo targets of the mammalian Cdc34 and Rad6 ubiquitin-conjugating enzymes are known. A yeast-based genetic assay to identify proteins that interact with human Cdc34 resulted in three cDNAs encoding bZIP DNA binding ...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/mcb.19.7.5001

    authors： Pati D,Meistrich ML,Plon SE

    更新日期：1999-07-01 00:00:00

  • CAP is a bifunctional component of the Saccharomyces cerevisiae adenylyl cyclase complex.

    abstract：:CAP, a protein from Saccharomyces cerevisiae that copurifies with adenylyl cyclase, appears to be required for yeast cells to be fully responsive to RAS proteins. CAP also appears to be required for normal cell morphology and responsiveness to nutrient deprivation and excess. We describe here a molecular and phenotypi...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/mcb.11.3.1248

    authors： Gerst JE,Ferguson K,Vojtek A,Wigler M,Field J

    更新日期：1991-03-01 00:00:00

  • Simian virus 40 large T-antigen point mutants that are defective in viral DNA replication but competent in oncogenic transformation.

    abstract：:The large T antigen of simian virus 40 (SV40) is a multifunctional protein that is essential in both the virus lytic cycle and the oncogenic transformation of cells by SV40. To investigate the role of the numerous biochemical and physiological activities of T antigen in the lytic and transformation processes, we have ...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/mcb.4.6.1125

    authors： Manos MM,Gluzman Y

    更新日期：1984-06-01 00:00:00

  • DEAD Box 1 Facilitates Removal of RNA and Homologous Recombination at DNA Double-Strand Breaks.

    abstract：:Although RNA and RNA-binding proteins have been linked to double-strand breaks (DSBs), little is known regarding their roles in the cellular response to DSBs and, if any, in the repair process. Here, we provide direct evidence for the presence of RNA-DNA hybrids at DSBs and suggest that binding of RNA to DNA at DSBs m...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/MCB.00415-16

    authors： Li L,Germain DR,Poon HY,Hildebrandt MR,Monckton EA,McDonald D,Hendzel MJ,Godbout R

    更新日期：2016-10-28 00:00:00

  • A ubiquitin mutant with specific defects in DNA repair and multiubiquitination.

    abstract：:The degradation of many proteins involves the sequential ligation of ubiquitin molecules to the substrate to form a multiubiquitin chain linked through Lys-48 of ubiquitin. To test for the existence of alternate forms of multiubiquitin chains, we examined the effects of individually substituting each of six other Lys ...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/mcb.15.3.1265

    authors： Spence J,Sadis S,Haas AL,Finley D

    更新日期：1995-03-01 00:00:00

  • Regulation of urokinase-type plasminogen activator gene transcription by macrophage colony-stimulating factor.

    abstract：:The mouse urokinase-type plasminogen activator (uPA) gene was used as a model macrophage colony-stimulating factor 1 (CSF-1)-inducible gene to investigate CSF-1 signalling pathways. Nuclear run-on analysis showed that induction of uPA mRNA by CSF-1 and phorbol myristate acetate (PMA) was at the transcriptional level i...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/mcb.15.6.3430

    authors： Stacey KJ,Fowles LF,Colman MS,Ostrowski MC,Hume DA

    更新日期：1995-06-01 00:00:00

  • The Drosophila polycomb protein interacts with nucleosomal core particles In vitro via its repression domain.

    abstract：:The proteins of the Polycomb group (PcG) are required for maintaining regulator genes, such as the homeotic selectors, stably and heritably repressed in appropriate developmental domains. It has been suggested that PcG proteins silence genes by creating higher-order chromatin structures at their chromosomal targets, t...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/mcb.19.12.8451

    authors： Breiling A,Bonte E,Ferrari S,Becker PB,Paro R

    更新日期：1999-12-01 00:00:00

  • Isolation and characterization of calmodulin genes from Xenopus laevis.

    abstract：:Two cDNAs derived from Xenopus laevis calmodulin mRNA have been cloned. Both cDNAs contain the complete protein-coding region and various lengths of untranslated segments. The two cDNAs encode an identical protein but differ from each other by 5% nucleotide substitutions. The 5' and 3' untranslated regions, to the ext...

    journal_title：Molecular and cellular biology

    pub_type： 杂志文章

    doi：10.1128/mcb.4.3.507

    authors： Chien YH,Dawid IB

    更新日期：1984-03-01 00:00:00