Fragments of ATM which have dominant-negative or complementing activity.

abstract：:A protein that binds specifically to Saccharomyces cerevisiae centromere DNA element I was purified on the basis of a nitrocellulose filter-binding assay. This protein, termed centromere-binding protein 1 (CP1), was heat stable and renaturable from sodium dodecyl sulfate (SDS), and assays of eluates from SDS gels indi...

journal_title：Molecular and cellular biology

authors： Bram RJ,Kornberg RD

更新日期：1987-01-01 00:00:00

abstract：:To explore the diversity in the mechanisms of basal transcription by RNA polymerase II, we have employed a novel biochemical approach that involves perturbation of the transcription reaction with exogenously added TFIIB or TATA box-binding protein (TBP). Under these conditions, we observe promoter-selective inhibition...

journal_title：Molecular and cellular biology

authors： George CP,Lira-DeVito LM,Wampler SL,Kadonaga JT

更新日期：1995-02-01 00:00:00

abstract：:We have investigated the kinds of mutations induced when a shuttle vector containing covalently bound residues of the (+/-)-7 beta,8 alpha-dihydroxy-9 alpha,10 alpha-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE) replicates in the monkey kidney cell line COS7. The target for detecting mutations was the 200-base pair g...

journal_title：Molecular and cellular biology

authors： Yang JL,Maher VM,McCormick JJ

更新日期：1987-03-01 00:00:00

abstract：:In Saccharomyces cerevisiae, many amino acid biosynthetic pathways are coregulated by a complex general control system: starvation for a single amino acid results in the derepression of amino acid biosynthetic genes in multiple pathways. Derepression of these genes is mediated by positive (GCN) and negative (GCD) regu...

journal_title：Molecular and cellular biology

authors： Myers PL,Skvirsky RC,Greenberg ML,Greer H

更新日期：1986-09-01 00:00:00

abstract：:Sequences of DNA that hybridize on Southern blots with cloned intracisternal A-particle (IAP) sequences have been examined in genomic DNAs of neonatal mice, livers of adult mice (3, 6, 12, 18, 24, and 26 months old), and the solid myeloma tumor MOPC-315. The isoschizomers HpaII (CCGG or mCCGG) and MspI (CCGG or CmCGG)...

journal_title：Molecular and cellular biology

authors： Mays-Hoopes LL,Brown A,Huang RC

更新日期：1983-08-01 00:00:00

abstract：:Apoptotic cells are swiftly engulfed by macrophages to prevent the release of noxious materials from dying cells. Apoptotic cells expose phosphatidylserine (PtdSer) on their surface, and macrophages engulf them by recognizing PtdSer using specific receptors and opsonins. Here, we found that mouse resident peritoneal m...

journal_title：Molecular and cellular biology

authors： Nishi C,Toda S,Segawa K,Nagata S

更新日期：2014-04-01 00:00:00

abstract：:SCF complexes are E3 ubiquitin-protein ligases that mediate degradation of regulatory and signaling proteins and control G1/S cell cycle progression by degradation of G1 cyclins and the cyclin-dependent kinase inhibitor, Sic1. Interchangeable F-box proteins bind the core SCF components; each recruits a specific subset...

journal_title：Molecular and cellular biology

authors： Ivantsiv Y,Kaplun L,Tzirkin-Goldin R,Shabek N,Raveh D

更新日期：2006-03-01 00:00:00

abstract：:Retinoblastoma (RB) tumors develop when both alleles of a gene (RB1) are mutated and unable to function normally. Recently, Friend et al. [S. H. Friend, R. Bernards, S. Rogelj, R. A. Weinberg, J. M. Rapaport, D. M. Albert, and T. P. Dryja, Nature (London) 32:643-646, 1986] reported the cloning of a gene, 4.7R, with so...

journal_title：Molecular and cellular biology

authors： Goddard AD,Balakier H,Canton M,Dunn J,Squire J,Reyes E,Becker A,Phillips RA,Gallie BL

更新日期：1988-05-01 00:00:00

abstract：:Transfection of C3H/10T1/2 cells with a c-myc gene resulted in the acquisition of responsiveness to transforming growth factor type beta. Cells transfected with an activated H-ras gene or an H-ras and c-myc gene, however, exhibited a transformed morphology and spontaneous soft-agar growth, a phenotype induced reversib...

journal_title：Molecular and cellular biology

authors： Leof EB,Proper JA,Moses HL

更新日期：1987-07-01 00:00:00

abstract：:Nucleotide excision repair and the long-patch mismatch repair systems correct abnormal DNA structures arising from DNA damage and replication errors, respectively. DNA synthesis past a damaged base (translesion replication) often causes misincorporation at the lesion site. In addition, mismatches are hot spots for DNA...

journal_title：Molecular and cellular biology

authors： Mu D,Tursun M,Duckett DR,Drummond JT,Modrich P,Sancar A

更新日期：1997-02-01 00:00:00

abstract：:Telomere maintenance is required for chromosome stability, and telomeres are typically replicated by the action of the reverse transcriptase telomerase. In both tumor and yeast cells that lack telomerase, telomeres are maintained by an alternative recombination mechanism. Genetic studies have led to the identification...

journal_title：Molecular and cellular biology

authors： Tsai YL,Tseng SF,Chang SH,Lin CC,Teng SC

更新日期：2002-08-01 00:00:00

abstract：:The steady-state mRNA levels of the proliferating cell nuclear antigen (PCNA) gene are growth regulated. In a previous paper (L. Ottavio, C.-D. Chang, M. G. Rizzo, S. Travali, C. Casadevall, and R. Baserga, Mol. Cell. Biol. 10:303-309, 1990), we reported that introns (especially intron 4) participate in growth regulat...

journal_title：Molecular and cellular biology

authors： Chang CD,Ottavio L,Travali S,Lipson KE,Baserga R

更新日期：1990-07-01 00:00:00

abstract：:In a gene trap screen for genes expressed in the primitive streak and tail bud during mouse embryogenesis, we isolated a mutation in Jade1, a gene encoding a PHD zinc finger protein previously shown to interact with the tumor suppressor pVHL. Expressed sequence tag analysis indicates that Jade1 is subject to posttrans...

journal_title：Molecular and cellular biology

authors： Tzouanacou E,Tweedie S,Wilson V

更新日期：2003-12-01 00:00:00

abstract：:The expression of Id1, a helix-loop-helix protein which inhibits the activity of basic helix-loop-helix transcription factors, is down-regulated during cellular differentiation and cell cycle withdrawal both in tissue culture models and in mouse embryos. In order to study the mechanism of control of Idl expression, we...

journal_title：Molecular and cellular biology

authors： Tournay O,Benezra R

更新日期：1996-05-01 00:00:00

abstract：:The murine Igh locus has a 3' regulatory region (3' RR) containing four enhancers (hs3A, hs1,2, hs3B, and hs4) at DNase I-hypersensitive sites. The 3' RR exerts long-range effects on class switch recombination (CSR) to several isotypes through its control of germ line transcription. By measuring levels of acetylated h...

journal_title：Molecular and cellular biology

authors： Garrett FE,Emelyanov AV,Sepulveda MA,Flanagan P,Volpi S,Li F,Loukinov D,Eckhardt LA,Lobanenkov VV,Birshtein BK

更新日期：2005-02-01 00:00:00

abstract：:The human glycoprotein IIB (GPIIB) gene is expressed only in megakaryocytes, and its promoter displays cell type specificity. We show that this specificity involved two cis-acting sequences. The first one, located at -55, contains a GATA binding site. Point mutations that abolish protein binding on this site decrease ...

journal_title：Molecular and cellular biology

authors： Lemarchandel V,Ghysdael J,Mignotte V,Rahuel C,Roméo PH

更新日期：1993-01-01 00:00:00

abstract：:The induction of the granulocytic differentiation of leukemic cells by all-trans retinoic acid (RA) has been a major breakthrough in terms of survival for acute promyelocytic leukemia (APL) patients. Here we highlight the synergism and the underlying novel mechanism between RA and the granulocyte colony-stimulating fa...

journal_title：Molecular and cellular biology

authors： Cassinat B,Zassadowski F,Ferry C,Llopis L,Bruck N,Lainey E,Duong V,Cras A,Despouy G,Chourbagi O,Beinse G,Fenaux P,Rochette Egly C,Chomienne C

更新日期：2011-04-01 00:00:00

abstract：:When herpes simplex virus infects permissive cells, the viral regulatory protein VP16 forms a specific complex with HCF-1, a preexisting nuclear protein involved in cell proliferation. The majority of HCF-1 in the cell is a complex of associated amino (HCF-1(N))- and carboxy (HCF-1(C))-terminal subunits that result fr...

journal_title：Molecular and cellular biology

authors： Wilson AC,Boutros M,Johnson KM,Herr W

更新日期：2000-09-01 00:00:00

abstract：:The B-lymphocyte- and macrophage-specific transcription factor PU.1 is a member of the ets family of proteins. To understand how PU.1 functions as a transcription factor, we initiated a series of experiments to define its activation domain. Using deletion analysis, we showed that the activation domain of PU.1 is locat...

journal_title：Molecular and cellular biology

authors： Klemsz MJ,Maki RA

更新日期：1996-01-01 00:00:00

abstract：:Cwc25 has previously been identified to associate with pre-mRNA splicing factor Cef1/Ntc85, a component of the Prp19-associated complex (nineteen complex, or NTC) involved in spliceosome activation. We show here that Cwc25 is neither tightly associated with NTC nor required for spliceosome activation but is required f...

journal_title：Molecular and cellular biology

authors： Chiu YF,Liu YC,Chiang TW,Yeh TC,Tseng CK,Wu NY,Cheng SC

更新日期：2009-11-01 00:00:00

abstract：:Mutations in mitochondrial DNA (mtDNA) cause a variety of relatively rare human diseases and may contribute to the pathogenesis of other, more common degenerative diseases. This stimulates interest in the capacity of mitochondria to repair damage to mtDNA. Several recent studies have shown that some types of damage to...

journal_title：Molecular and cellular biology

authors： Pinz KG,Bogenhagen DF

更新日期：1998-03-01 00:00:00

abstract：:We show that the nuclear genes for the cytoplasmic and mitochondrial leucyl-tRNA synthetase (LeuRS) of Neurospora crassa are distinct in their encoded proteins, codon usage, mRNA levels, and regulation. The 4.2-kilobase-pair region representing the structural gene for cytoplasmic LeuRS and flanking regions has been se...

journal_title：Molecular and cellular biology

authors： Chow CM,Rajbhandary UL

更新日期：1989-11-01 00:00:00

abstract：:Microinjection of p21Ha-ras proteins into Xenopus laevis oocytes induces a rapid increase of 1,2-diacylglycerol (DAG) levels. The observed alterations in DAG levels were consistent with the ability of the protein to induce maturation, measured by germinal vesicle breakdown (GVBD). Both the increase in DAG levels and G...

journal_title：Molecular and cellular biology

authors： Lacal JC

更新日期：1990-01-01 00:00:00

abstract：:Compromised immunoregulation contributes to obesity and complications in metabolic pathogenesis. Here, we demonstrate that the nuclear factor of activated T cell (NFAT) group of transcription factors contributes to glucose and insulin homeostasis. Expression of two members of the NFAT family (NFATc2 and NFATc4) is ind...

journal_title：Molecular and cellular biology

authors： Yang TT,Suk HY,Yang X,Olabisi O,Yu RY,Durand J,Jelicks LA,Kim JY,Scherer PE,Wang Y,Feng Y,Rossetti L,Graef IA,Crabtree GR,Chow CW

更新日期：2006-10-01 00:00:00

abstract：:The simian virus 40 72-base-pair repeats substituted for the polyomavirus enhancer, allowing replication and transcription in mouse 3T6 but not monkey CV-1 cells. A polyomavirus genome containing the entire simian virus 40 control region replicated at low levels in 3T6 and CV-1 cells; however, transcripts were detecte...

journal_title：Molecular and cellular biology

authors： Campbell BA,Villarreal LP

更新日期：1985-06-01 00:00:00

abstract：:Transcriptional coactivators play a crucial role in gene expression by communicating between regulatory factors and the basal transcription machinery. The coactivator multiprotein bridging factor 1 (MBF1) was originally identified as a bridging molecule that connects the Drosophila nuclear receptor FTZ-F1 and TATA-bin...

journal_title：Molecular and cellular biology

authors： Takemaru K,Harashima S,Ueda H,Hirose S

更新日期：1998-09-01 00:00:00

abstract：:Oxygen radicals regulate many physiological processes, such as signaling, proliferation, and apoptosis, and thus play a pivotal role in pathophysiology and disease development. There are at least two thioredoxin reductase/thioredoxin/peroxiredoxin systems participating in the cellular defense against oxygen radicals. ...

journal_title：Molecular and cellular biology

authors： Conrad M,Jakupoglu C,Moreno SG,Lippl S,Banjac A,Schneider M,Beck H,Hatzopoulos AK,Just U,Sinowatz F,Schmahl W,Chien KR,Wurst W,Bornkamm GW,Brielmeier M

更新日期：2004-11-01 00:00:00

abstract：:A C/EBP-like transcription factor, AGP/EBP, that binds to three distinct motifs in the 5'-flanking region of alpha 1-acid glycoprotein gene (AGP) has been identified. Here we report the cloning and properties of cDNA corresponding to mouse AGP/EBP. AGP/EBP and C/EBP share 87% amino acid sequence homology in the "leuci...

journal_title：Molecular and cellular biology

authors： Chang CJ,Chen TT,Lei HY,Chen DS,Lee SC

更新日期：1990-12-01 00:00:00

abstract：:The Myt1 protein kinase functions to negatively regulate Cdc2-cyclin B complexes by phosphorylating Cdc2 on threonine 14 and tyrosine 15. Throughout interphase, human Myt1 localizes to the endoplasmic reticulum and Golgi complex, whereas Cdc2-cyclin B1 complexes shuttle between the nucleus and the cytoplasm. Here we r...

journal_title：Molecular and cellular biology

authors： Liu F,Rothblum-Oviatt C,Ryan CE,Piwnica-Worms H

更新日期：1999-07-01 00:00:00

abstract：:SEC63 encodes a protein required for secretory protein translocation into the endoplasmic reticulum (ER) of Saccharomyces cerevisiae (J. A. Rothblatt, R. J. Deshaies, S. L. Sanders, G. Daum, and R. Schekman, J. Cell Biol. 109:2641-2652, 1989). Antibody directed against a recombinant form of the protein detects a 73-kD...

journal_title：Molecular and cellular biology

authors： Feldheim D,Rothblatt J,Schekman R

更新日期：1992-07-01 00:00:00