HCF-1 amino- and carboxy-terminal subunit association through two separate sets of interaction modules: involvement of fibronectin type 3 repeats.


:When herpes simplex virus infects permissive cells, the viral regulatory protein VP16 forms a specific complex with HCF-1, a preexisting nuclear protein involved in cell proliferation. The majority of HCF-1 in the cell is a complex of associated amino (HCF-1(N))- and carboxy (HCF-1(C))-terminal subunits that result from an unusual proteolytic processing of a large precursor polypeptide. Here, we have characterized the structure and function of sequences required for HCF-1(N) and HCF-1(C) subunit association. HCF-1 contains two matched pairs of self-association sequences called SAS1 and SAS2. One of these matched association sequences, SAS1, consists of a short 43-amino-acid region of the HCF-1(N) subunit, which associates with a carboxy-terminal region of the HCF-1(C) subunit that is composed of a tandem pair of fibronectin type 3 repeats, a structural motif known to promote protein-protein interactions. Unexpectedly, the related protein HCF-2, which is not proteolyzed, also contains a functional SAS1 association element, suggesting that this element does not function solely to maintain HCF-1(N) and HCF-1(C) subunit association. HCF-1(N) subunits do not possess a nuclear localization signal. We show that, owing to a carboxy-terminal HCF-1 nuclear localization signal, HCF-1(C) subunits can recruit HCF-1(N) subunits to the nucleus.


Mol Cell Biol


Wilson AC,Boutros M,Johnson KM,Herr W




Has Abstract


2000-09-01 00:00:00












  • Spatiotemporally regulated protein kinase A activity is a critical regulator of growth factor-stimulated extracellular signal-regulated kinase signaling in PC12 cells.

    abstract::PC12 cells exhibit precise temporal control of growth factor signaling in which stimulation with epidermal growth factor (EGF) leads to transient extracellular signal-regulated kinase (ERK) activity and cell proliferation, whereas nerve growth factor (NGF) stimulation leads to sustained ERK activity and differentiatio...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Herbst KJ,Allen MD,Zhang J

    更新日期:2011-10-01 00:00:00

  • A family of insulin-like growth factor II mRNA-binding proteins represses translation in late development.

    abstract::Insulin-like growth factor II (IGF-II) is a major fetal growth factor. The IGF-II gene generates multiple mRNAs with different 5' untranslated regions (5' UTRs) that are translated in a differential manner during development. We have identified a human family of three IGF-II mRNA-binding proteins (IMPs) that exhibit m...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Nielsen J,Christiansen J,Lykke-Andersen J,Johnsen AH,Wewer UM,Nielsen FC

    更新日期:1999-02-01 00:00:00

  • A Swiss 3T3 variant cell line resistant to the effects of tumor promoters cannot be transformed by src.

    abstract::To study the relationship between oncogenesis by v-src and normal cellular signalling pathways, we determined the effects of v-src on 3T3-TNR9 cells, a Swiss 3T3 variant which does not respond mitogenically to tumor promoters such as 12-O-tetradecanoyl-phorbol-13-acetate (TPA). We found that src was unable to transfor...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Nori M,Shawver LK,Weber MJ

    更新日期:1990-08-01 00:00:00

  • Identification of cis and trans components of a novel heat shock stress regulatory pathway in Saccharomyces cerevisiae.

    abstract::The stress-responsive DDR2 gene (previously called DDRA2) of Saccharomyces cerevisiae is transcribed at elevated levels following stress caused by heat shock or DNA damage. Previously, we identified a 51-bp promoter fragment, oligo31/32, which conferred heat shock inducibility on the heterologous CYC1-lacZ reporter ge...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Kobayashi N,McEntee K

    更新日期:1993-01-01 00:00:00

  • Stb1 collaborates with other regulators to modulate the G1-specific transcriptional circuit.

    abstract::G(1)-specific transcription in the budding yeast Saccharomyces cerevisiae depends upon SBF and MBF. Whereas inactivation of SBF-regulated genes during the G(1)/S transition depends upon mitotic B-type cyclins, inactivation of MBF has been reported to involve multiple regulators, Nrm1 and Stb1. Nrm1 is a transcriptiona...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: de Bruin RA,Kalashnikova TI,Wittenberg C

    更新日期:2008-11-01 00:00:00

  • Unusual levels of heat shock element-binding activity in embryonal carcinoma cells.

    abstract::In contrast to differentiated somatic cells, mouse embryonal carcinoma (EC) cell lines spontaneously express high levels of major members of the heat shock protein (HSP) family. In addition, some EC cell lines (noninducible) are not able to induce HSP gene transcription and HSP synthesis after a stress. However, after...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Mezger V,Bensaude O,Morange M

    更新日期:1989-09-01 00:00:00

  • Organizational analysis of elav gene and functional analysis of ELAV protein of Drosophila melanogaster and Drosophila virilis.

    abstract::Drosophila virilis genomic DNA corresponding to the D. melanogaster embryonic lethal abnormal visual system (elav) locus was cloned. DNA sequence analysis of a 3.8-kb genomic piece allowed identification of (i) an open reading frame (ORF) with striking homology to the previously identified D. melanogaster ORF and (ii)...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Yao KM,White K

    更新日期:1991-06-01 00:00:00

  • A human globin enhancer causes both discrete and widespread alterations in chromatin structure.

    abstract::Gene activation requires alteration of chromatin structure to facilitate active transcription complex formation at a gene promoter. Nucleosome remodeling complexes and histone modifying complexes each play unique and interdependent roles in bringing about these changes. The role of distant enhancers in these structura...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Kim A,Dean A

    更新日期:2003-11-01 00:00:00

  • The AD1 and AD2 transactivation domains of E2A are essential for the antiapoptotic activity of the chimeric oncoprotein E2A-HLF.

    abstract::The chimeric oncoprotein E2A-HLF, generated by the t(17;19) chromosomal translocation in pro-B-cell acute lymphoblastic leukemia, incorporates the transactivation domains of E2A and the basic leucine zipper (bZIP) DNA-binding and protein dimerization domain of HLF (hepatic leukemic factor). The ability of E2A-HLF to p...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Inukai T,Inaba T,Ikushima S,Look AT

    更新日期:1998-10-01 00:00:00

  • mRNA instability in the nucleus due to a novel open reading frame element is a major determinant of the narrow tissue specificity of folate receptor alpha.

    abstract::The folate receptor type alpha (FR-alpha) is a promising tumor marker and target. Here, we investigate the mechanistic basis for the tumor specificity and vast overexpression of FR-alpha. Among representative FR-alpha-positive (HeLa and JAR) and FR-alpha-negative (MG63, Caki1, and HT3) cell lines, the transcription ra...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Zheng X,Kelley K,Elnakat H,Yan W,Dorn T,Ratnam M

    更新日期:2003-03-01 00:00:00

  • At the revolution with Fred Sherman.

    abstract::Fred Sherman was a prominent yeast geneticist and my mentor in graduate school. Fred passed away in September 2013 at the age of 81. In this minireview, I describe what it was like to know Fred and be in his lab from 1977 to 1982, the extraordinarily exciting time when the recombinant DNA revolution hit yeast genetics...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章,评审


    authors: Zaret KS

    更新日期:2014-03-01 00:00:00

  • GGAPs, a new family of bifunctional GTP-binding and GTPase-activating proteins.

    abstract::G proteins are molecular switches that control a wide variety of physiological functions, including neurotransmission, transcriptional activation, cell migration, cell growth. and proliferation. The ability of GTPases to participate in signaling events is determined by the ratio of GTP-bound to GDP-bound forms in the ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Xia C,Ma W,Stafford LJ,Liu C,Gong L,Martin JF,Liu M

    更新日期:2003-04-01 00:00:00

  • Oncogenic ras and p53 cooperate to induce cellular senescence.

    abstract::Oncogenic activation of the mitogen-activated protein (MAP) kinase cascade in murine fibroblasts initiates a senescence-like cell cycle arrest that depends on the ARF/p53 tumor suppressor pathway. To investigate whether p53 is sufficient to induce senescence, we introduced a conditional murine p53 allele (p53(val135))...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Ferbeyre G,de Stanchina E,Lin AW,Querido E,McCurrach ME,Hannon GJ,Lowe SW

    更新日期:2002-05-01 00:00:00

  • Genomic instability and enhanced radiosensitivity in Hsp70.1- and Hsp70.3-deficient mice.

    abstract::Heat shock proteins (HSPs) are highly conserved among all organisms from prokaryotes to eukaryotes. In mice, the HSP genes Hsp70.1 and Hsp70.3 are induced by both endogenous and exogenous stressors, such as heat and toxicants. In order to determine whether such proteins specifically influence genomic instability, mice...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Hunt CR,Dix DJ,Sharma GG,Pandita RK,Gupta A,Funk M,Pandita TK

    更新日期:2004-01-01 00:00:00

  • A brain-specific homeobox gene, Bsx, is essential for proper postnatal growth and nursing.

    abstract::To investigate in vivo roles of a murine hypothalamic homeobox gene, Bsx, we generated and analyzed two mutant alleles, Bsx(DeltaHD) and Bsx(lacZ). Bsx(DeltaHD) lacks the homeodomain, and Bsx(lacZ) is an insertion of a lacZ reporter gene. Bsx-lacZ expression was detected in the hypothalamus and pineal gland and reiter...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: McArthur T,Ohtoshi A

    更新日期:2007-07-01 00:00:00

  • Nucleosome translational position, not histone acetylation, determines TFIIIA binding to nucleosomal Xenopus laevis 5S rRNA genes.

    abstract::We sought to study the binding constraints placed on the nine-zinc-finger protein transcription factor IIIA (TFIIIA) by a histone octamer. To this end, five overlapping fragments of the Xenopus laevis oocyte and somatic 5S rRNA genes were reconstituted into nucleosomes, and it was subsequently shown that nucleosome tr...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Howe L,Ausió J

    更新日期:1998-03-01 00:00:00

  • Molecular cloning of casein kinase II alpha subunit from Dictyostelium discoideum and its expression in the life cycle.

    abstract::A Dictyostelium discoideum cDNA encoding an alpha-type subunit of casein kinase II was isolated, and its cDNA was used to study developmental expression of casein kinase II during the Dictyostelium life cycle. The 1.3-kb cDNA insert contained an open reading frame of 337 amino acids (M(r) 39,900). The deduced amino ac...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Kikkawa U,Mann SK,Firtel RA,Hunter T

    更新日期:1992-12-01 00:00:00

  • Regulated expression of a complete human beta-globin gene encoded by a transmissible retrovirus vector.

    abstract::We introduced a human beta-globin gene into murine erythroleukemia (MEL) cells by infection with recombinant retroviruses containing the complete genomic globin sequence. The beta-globin gene was correctly regulated during differentiation, steady-state mRNA levels being induced 5- to 30-fold after treatment of the cel...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Cone RD,Weber-Benarous A,Baorto D,Mulligan RC

    更新日期:1987-02-01 00:00:00

  • Isolation of a cDNA clone complementary to sequences for a 34-kilodalton protein which is a pp60v-src substrate.

    abstract::We have isolated a partial cDNA clone containing sequences complementary to a mRNA encoding a 34- to 36-kilodalton normal chicken cell protein which is a substrate for pp60v-src kinase activity. Using this 34-kilodalton cDNA clone as a probe, we determined that the size of the 34-kilodalton mRNA was 1,100 nucleotides ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Tomasiewicz HG,Cook-Deegan R,Chikaraishi DM

    更新日期:1984-09-01 00:00:00

  • Progesterone receptor induces ErbB-2 nuclear translocation to promote breast cancer growth via a novel transcriptional effect: ErbB-2 function as a coactivator of Stat3.

    abstract::Progesterone receptor (PR) and ErbB-2 bidirectional cross talk participates in breast cancer development. Here, we identified a new mechanism of the PR and ErbB-2 interaction involving the PR induction of ErbB-2 nuclear translocation and the assembly of a transcriptional complex in which ErbB-2 acts as a coactivator o...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Béguelin W,Díaz Flaqué MC,Proietti CJ,Cayrol F,Rivas MA,Tkach M,Rosemblit C,Tocci JM,Charreau EH,Schillaci R,Elizalde PV

    更新日期:2010-12-01 00:00:00

  • A 12-base-pair sequence is an essential element of the ribosomal gene terminator in Xenopus laevis.

    abstract::rRNA transcription in Xenopus laevis terminates near a 7-base-pair (bp) conserved sequence (T3 box) located 200 bp upstream of the site of transcription initiation for the adjacent gene promoter. We present evidence here that a 12-bp element containing the T3 box is an essential part of the terminator. Using an oocyte...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Labhart P,Reeder RH

    更新日期:1987-05-01 00:00:00

  • DNA-binding and -bending activities of SAP30L and SAP30 are mediated by a zinc-dependent module and monophosphoinositides.

    abstract::Deacetylation of histones is carried out by a corepressor complex in which Sin3A is an essential scaffold protein. Two proteins in this complex, the Sin3A-associated proteins SAP30L and SAP30, have previously been suggested to function as linker molecules between various corepressors. In this report, we demonstrate ne...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Viiri KM,Jänis J,Siggers T,Heinonen TY,Valjakka J,Bulyk ML,Mäki M,Lohi O

    更新日期:2009-01-01 00:00:00

  • Tiam1 regulates the Wnt/Dvl/Rac1 signaling pathway and the differentiation of midbrain dopaminergic neurons.

    abstract::Understanding the mechanisms that drive the differentiation of dopaminergic (DA) neurons is crucial for successful development of novel therapies for Parkinson's disease, in which DA neurons progressively degenerate. However, the mechanisms underlying the differentiation-promoting effects of Wnt5a on DA precursors are...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Čajánek L,Ganji RS,Henriques-Oliveira C,Theofilopoulos S,Koník P,Bryja V,Arenas E

    更新日期:2013-01-01 00:00:00

  • A conserved docking motif for CK1 binding controls the nuclear localization of NFAT1.

    abstract::In resting cells, the NFAT1 transcription factor is kept inactive in the cytoplasm by phosphorylation on multiple serine residues. These phosphorylated residues are primarily contained within two types of serine-rich motifs, the SRR-1 and SP motifs, which are conserved within the NFAT family. Several different kinases...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Okamura H,Garcia-Rodriguez C,Martinson H,Qin J,Virshup DM,Rao A

    更新日期:2004-05-01 00:00:00

  • Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.

    abstract::We constructed a series of recombinant genomes which directed expression of the enzyme chloramphenicol acetyltransferase (CAT) in mammalian cells. The prototype recombinant in this series, pSV2-cat, consisted of the beta-lactamase gene and origin of replication from pBR322 coupled to a simian virus 40 (SV40) early tra...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Gorman CM,Moffat LF,Howard BH

    更新日期:1982-09-01 00:00:00

  • Common factor 1 is a transcriptional activator which binds in the c-myc promoter, the skeletal alpha-actin promoter, and the immunoglobulin heavy-chain enhancer.

    abstract::Ubiquitously expressed transcription factors play an integral role in establishing and regulating patterns of gene transcription. Common factor 1 (CF1) is a ubiquitously expressed DNA-binding protein previously identified in our laboratory. We show here that CF1 recognizes sites in several diverse transcription elemen...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Riggs KJ,Merrell KT,Wilson G,Calame K

    更新日期:1991-03-01 00:00:00

  • MdmX promotes bipolar mitosis to suppress transformation and tumorigenesis in p53-deficient cells and mice.

    abstract::Mdm2 and MdmX are structurally related p53-binding proteins that function as critical negative regulators of p53 activity in embryonic and adult tissue. The overexpression of Mdm2 or MdmX inhibits p53 tumor suppressor functions in vitro, and the amplification of Mdm2 or MdmX is observed in human cancers retaining wild...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Matijasevic Z,Steinman HA,Hoover K,Jones SN

    更新日期:2008-02-01 00:00:00

  • The Drosophila dorsal morphogen represses the tolloid gene by interacting with a silencer element.

    abstract::The dorsal protein (DL) regulates the transcriptional activity of several genes that determine cell fate along the dorsoventral axis of the Drosophila melanogaster embryo. DL is present at high levels in ventral nuclei, where it activates some genes (twi and sna) and represses others (zen, dpp, and tld). DL shows homo...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Kirov N,Childs S,O'Connor M,Rushlow C

    更新日期:1994-01-01 00:00:00

  • Sequences far downstream from the classical tRNA promoter elements bind RNA polymerase III transcription factors.

    abstract::We have examined the interaction of transcription factors TFIIIC and TFIIID with a silkworm alanine tRNA gene. Previous functional analysis showed that the promoter for this gene is unusually large compared with the classical tRNA promoter elements (the A and B boxes) and includes sequences downstream from the transcr...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Young LS,Rivier DH,Sprague KU

    更新日期:1991-03-01 00:00:00

  • Alternative excision products originating from a single integration of polyomavirus DNA.

    abstract::The Cyp cell line consists of mouse cells transformed by a thermosensitive polyomavirus (Py) genome and routinely propagated at 39 degrees C. Cyp cells are readily induced to synthesize free Py DNA by being transferred to 33 degrees C. In one subclone (C12/a1/S48, or S48) of this line, such induction resulted in the i...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Huberdeau D,Sylla BS,Herring-Gillam E,Bourgaux-Ramoisy D,Bourgaux P

    更新日期:1985-10-01 00:00:00