Abstract:
:Transcriptional coactivators play a crucial role in gene expression by communicating between regulatory factors and the basal transcription machinery. The coactivator multiprotein bridging factor 1 (MBF1) was originally identified as a bridging molecule that connects the Drosophila nuclear receptor FTZ-F1 and TATA-binding protein (TBP). The MBF1 sequence is highly conserved across species from Saccharomyces cerevisiae to human. Here we provide evidence acquired in vitro and in vivo that yeast MBF1 mediates GCN4-dependent transcriptional activation by bridging the DNA-binding region of GCN4 and TBP. These findings indicate that the coactivator MBF1 functions by recruiting TBP to promoters where DNA-binding regulators are bound.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Takemaru K,Harashima S,Ueda H,Hirose Sdoi
10.1128/mcb.18.9.4971subject
Has Abstractpub_date
1998-09-01 00:00:00pages
4971-6issue
9eissn
0270-7306issn
1098-5549journal_volume
18pub_type
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