Abstract:
:In yeast cells, mutations in the TATA-binding protein (TBP) that disrupt the interaction with the TATA element or with TFIIA can selectively impair the response to acidic activator proteins. We analyzed the transcriptional properties of TBP derivatives in which residues that directly interact with TFIIB were replaced by alanines. Surprisingly, a derivative with a 50-fold defect in TBP-TFIIB-TATA complex formation in vitro (E188A) supports viability and responds efficiently to activators in vivo. The E186A derivative, which displays a 100-fold defect in TBP-TFIIB-TATA complex formation, does not support viability, yet it does respond to activators. Conversely, the L189A mutation, which has the mildest effect on the interaction with TFIIB (10-fold), can abolish transcriptional activation and cell viability when combined with mutations on the DNA-binding surface. This "synthetic lethal" effect is not observed with E188A, suggesting that the previously described role of L189 in transcriptional activation may be related to its location on the DNA-binding surface and not to its interaction with TFIIB. Finally, when using TBP mutants defective on multiple interaction surfaces, we observed synthetic lethal effects between mutations on the TFIIA and TFIIB interfaces but found that mutations implicated in association with polymerase II and TFIIF did not have significant effects in vivo. Taken together, these results argue that, unlike the TBP-TATA and TBP-TFIIA interactions, the TBP-TFIIB interaction is not generally limiting for transcriptional activation in vivo.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Lee M,Struhl Kdoi
10.1128/mcb.17.3.1336subject
Has Abstractpub_date
1997-03-01 00:00:00pages
1336-45issue
3eissn
0270-7306issn
1098-5549journal_volume
17pub_type
杂志文章abstract::The basis for nucleolar dominance in mouse-human cell hybrids which contained all of the mouse chromosomes but an incomplete set of human chromosomes (M greater than H) was examined at the molecular level. S1 mapping data showed that these cells had the expected levels of steady-state rRNA transcribed from mouse ribos...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:1987-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-10-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1991-01-01 00:00:00
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更新日期:1990-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-03-01 00:00:00
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更新日期:2003-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-09-01 00:00:00
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更新日期:2003-12-01 00:00:00
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更新日期:2005-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1997-05-01 00:00:00