Dynamics of human immunodeficiency virus transcription: P-TEFb phosphorylates RD and dissociates negative effectors from the transactivation response element.

abstract：:Using in vitro replication assays, we compared native with salt-treated simian virus 40 minichromosomes isolated from infected cell nuclei. Minichromosomes from both preparations contain the full complement of nucleosomes, but salt treatment removes histone H1 and a fraction of nonhistone chromatin proteins. Both type...

journal_title：Molecular and cellular biology

authors： Krude T,de Maddalena C,Knippers R

更新日期：1993-02-01 00:00:00

abstract：:CD83 is one of the best-known surface markers for fully mature dendritic cells (mature DCs), and its cell-type- and maturation-specific regulation makes the CD83 promoter an interesting tool for the genetic modulation of DCs. To determine the mechanisms regulating this DC- and maturation-specific CD83 expression, chro...

journal_title：Molecular and cellular biology

authors： Stein MF,Lang S,Winkler TH,Deinzer A,Erber S,Nettelbeck DM,Naschberger E,Jochmann R,Stürzl M,Slany RK,Werner T,Steinkasserer A,Knippertz I

更新日期：2013-04-01 00:00:00

abstract：:Gonadotropin-releasing hormone (GnRH) interacts with a G protein-coupled receptor and increases the transcription of the glycoprotein hormone alpha-subunit gene. We have explored the possibility that mitogen-activated protein kinase (MAPK) plays a role in mediating GnRH effects on transcription. Activation of the MAPK...

journal_title：Molecular and cellular biology

authors： Roberson MS,Misra-Press A,Laurance ME,Stork PJ,Maurer RA

更新日期：1995-07-01 00:00:00

abstract：:The chemotropic guidance cue netrin-1 mediates attraction of migrating axons during central nervous system development through the receptor Deleted in Colorectal Cancer (DCC). Downstream of netrin-1, activated Rho GTPases Rac1 and Cdc42 induce cytoskeletal rearrangements within the growth cone. The Rho guanine nucleot...

journal_title：Molecular and cellular biology

authors： DeGeer J,Boudeau J,Schmidt S,Bedford F,Lamarche-Vane N,Debant A

更新日期：2013-02-01 00:00:00

abstract：:In previous studies, we detected a major, unidentified Egr response element (ERE) binding complex in brain extracts. We now report that this complex contains a truncated isoform of Egr3 generated by use of an alternate translation start site at methionine 106. Furthermore, the ERE binding complex previously thought to...

journal_title：Molecular and cellular biology

authors： O'Donovan KJ,Baraban JM

更新日期：1999-07-01 00:00:00

abstract：:Sphingomyelin is a major lipid in the bilayer of subcellular membranes of eukaryotic cells. Different sphingomyelinases catalyze the initial step in the catabolism of sphingomyelin, the hydrolysis to phosphocholine and ceramide. Sphingomyelinases have been postulated to generate ceramide as a lipophilic second messeng...

journal_title：Molecular and cellular biology

authors： Zumbansen M,Stoffel W

更新日期：2002-06-01 00:00:00

abstract：:Ribosome biogenesis is a highly regulated process ensuring that cell growth (increase in biomass) is coordinated with cell proliferation. The formation of eukaryotic ribosomes is a multistep process initiated by the transcription and processing of rRNA in the nucleolus. Concomitant with this, several preribosomal part...

journal_title：Molecular and cellular biology

authors： Castle CD,Cassimere EK,Lee J,Denicourt C

更新日期：2010-09-01 00:00:00

abstract：:Mammalian Ras GTPase-activating protein (GAP), p120 Ras-GAP, has been implicated as both a downregulator and effector of Ras proteins, but its precise role in Ras-mediated signal transduction pathways is unclear. To begin a genetic analysis of the role of p120 Ras-GAP we identified a homolog from the fruit fly Drosoph...

journal_title：Molecular and cellular biology

authors： Feldmann P,Eicher EN,Leevers SJ,Hafen E,Hughes DA

更新日期：1999-03-01 00:00:00

abstract：:We deduced the structure of the mouse profilin II gene. It contains five exons that can generate four different transcripts by alternative splicing. Two transcripts encode different profilin II isoforms (designated IIa and IIb) that have similar affinities for actin but different affinities for polyphosphoinositides a...

journal_title：Molecular and cellular biology

authors： Lambrechts A,Braun A,Jonckheere V,Aszodi A,Lanier LM,Robbens J,Van Colen I,Vandekerckhove J,Fässler R,Ampe C

更新日期：2000-11-01 00:00:00

abstract：:The human vitamin D-binding protein (hDBP) gene exists in a cluster of four liver-expressed genes. A minimal hDBP transgene, containing a defined set of liver-specific DNase I hypersensitive sites (HSs), is robustly expressed in mouse liver in a copy-number-dependent manner. Here we evaluate these HSs for function. De...

journal_title：Molecular and cellular biology

authors： Hiroki T,Liebhaber SA,Cooke NE

更新日期：2007-11-01 00:00:00

abstract：:The cys-3+ gene of Neurospora crassa encodes a bZIP (basic region-leucine zipper) regulatory protein that is essential for sulfur structural gene expression (e.g., ars-1+). Nuclear transcription assays confirmed that cys-3+ was under sulfur-regulated transcriptional control and that cys-3+ transcription was constituti...

journal_title：Molecular and cellular biology

authors： Paietta JV

更新日期：1992-04-01 00:00:00

abstract：:Little is known about the posttranslational control of the cyclin-dependent protein kinase (CDK) inhibitor p21. We describe here a transient phosphorylation of p21 in the G2/M phase. G2/M-phosphorylated p21 is short-lived relative to hypophosphorylated p21. p21 becomes nuclear during S phase, prior to its phosphorylat...

journal_title：Molecular and cellular biology

authors： Dash BC,El-Deiry WS

更新日期：2005-04-01 00:00:00

abstract：:Wound healing in certain individuals leads to the development of keloid tumors which exhibit abnormal collagen metabolism and an increased abundance of extracellular matrix components. Comparison of fibronectin levels in fibroblasts derived from keloids and normal dermis revealed a relative increase in intracellular a...

journal_title：Molecular and cellular biology

authors： Babu M,Diegelmann R,Oliver N

更新日期：1989-04-01 00:00:00

abstract：:We have investigated the relationship between Xenopus laevis c-mos (mosXe) and the cyclin B component of maturation-promoting factor. Microinjection of Xenopus oocytes with in vitro-synthesized RNAs encoding Xenopus cyclin B1 or cyclin B2 induces the progression of meiosis, characterized by germinal vesicle breakdown ...

journal_title：Molecular and cellular biology

authors： Freeman RS,Ballantyne SM,Donoghue DJ

更新日期：1991-03-01 00:00:00

abstract：:alpha 1-Antitrypsin (AT), the major elastase inhibitor in mammalian serum, is produced primarily in the liver. We have characterized AT gene structure and expression in the mouse species Mus caroli, which expresses high levels of AT in the kidneys as well as in the liver. Analysis of cDNA and genomic clones showed tha...

journal_title：Molecular and cellular biology

authors： Latimer JJ,Berger FG,Baumann H

更新日期：1990-02-01 00:00:00

abstract：:We describe here a new cell line, EL2, which spontaneously arose from primary rat embryo fibroblasts and has the distinctive property of being highly susceptible to a number of different transforming genes. The high susceptibility is expressed not only in high transformation frequencies but, most importantly, in an un...

journal_title：Molecular and cellular biology

authors： Liboi E,Caruso M,Basilico C

更新日期：1984-12-01 00:00:00

abstract：:Low oxygen gradients (hypoxia and anoxia) are important determinants of pathological conditions under which the tissue blood supply is deficient or defective, such as in solid tumors. We have been investigating the relationship between the activation of hypoxia-inducible factor 1 (HIF-1), the primary transcriptional r...

journal_title：Molecular and cellular biology

authors： Laderoute KR,Amin K,Calaoagan JM,Knapp M,Le T,Orduna J,Foretz M,Viollet B

更新日期：2006-07-01 00:00:00

abstract：:We investigated the interaction of phospholipase C-gamma (PLC-gamma) with wild-type and mutant forms of the platelet-derived growth factor (PDGF) beta-receptor both in vivo and in vitro. After PDGF treatment of CHO cell lines expressing wild-type or either of two mutant (delta Ki and Y825F) PDGF receptors, PLC-gamma b...

journal_title：Molecular and cellular biology

authors： Morrison DK,Kaplan DR,Rhee SG,Williams LT

更新日期：1990-05-01 00:00:00

abstract：:The turnover of the colony-stimulating factor 1 receptor (CSF-1R), the c-fms proto-oncogene product, is accelerated by ligand binding or by activators of protein kinase C (PKC), such as the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). The mechanisms of ligand- and TPA-induced downmodulation were shown to ...

journal_title：Molecular and cellular biology

authors： Downing JR,Roussel MF,Sherr CJ

更新日期：1989-07-01 00:00:00

abstract：:Etoposide, a nonintercalating antitumor drug, is a potent inhibitor of topoisomerase II activity. When Trypanosoma equiperdum is treated with etoposide, cleavable complexes are stabilized between topoisomerase II and kinetoplast DNA minicircles, a component of trypanosome mitochondrial DNA (T. A. Shapiro, V. A. Klein,...

journal_title：Molecular and cellular biology

authors： Shapiro TA

更新日期：1994-06-01 00:00:00

abstract：:G protein-coupled receptors (GPCRs) have been shown to stimulate extracellular regulated kinases (ERKs) through a number of linear pathways that are initiated by G(q/11) or G(i) proteins. We studied signaling to the ERK cascade by receptors that simultaneously activate both G protein subfamilies. In HEK293T cells, bra...

journal_title：Molecular and cellular biology

authors： Blaukat A,Barac A,Cross MJ,Offermanns S,Dikic I

更新日期：2000-09-01 00:00:00

abstract：:The HOG (high-osmolarity glycerol) mitogen-activated protein kinase (MAPK) pathway regulates the osmotic stress response in the yeast Saccharomyces cerevisiae. Three type 2C Ser/Thr phosphatases (PTCs), Ptc1, Ptc2, and Ptc3, have been isolated as negative regulators of this pathway. Previously, multicopy expression of...

journal_title：Molecular and cellular biology

authors： Warmka J,Hanneman J,Lee J,Amin D,Ota I

更新日期：2001-01-01 00:00:00

abstract：:In Saccharomyces cerevisiae (budding yeast), commitment to cell division in late G(1) is promoted by the G(1) cyclin Cln3 and its associated cyclin-dependent kinase, Cdc28. We show here that all known aspects of the function of Cln3 in G(1) phase, including control of cell size, pheromone sensitivity, cell cycle progr...

journal_title：Molecular and cellular biology

authors： Wijnen H,Landman A,Futcher B

更新日期：2002-06-01 00:00:00

abstract：:Total genomic DNA from a temperature-sensitive, colcemid-resistant Chinese hamster ovary (CHO) cell mutant expressing an electrophoretic variant beta-tubulin was used to transform wild-type CHO cells to colcemid-resistant cells at 37 degrees C. Southern blot analysis of the transformant demonstrated the three- to five...

journal_title：Molecular and cellular biology

authors： Whitfield C,Abraham I,Ascherman D,Gottesman MM

更新日期：1986-05-01 00:00:00

abstract：:When herpes simplex virus infects permissive cells, the viral regulatory protein VP16 forms a specific complex with HCF-1, a preexisting nuclear protein involved in cell proliferation. The majority of HCF-1 in the cell is a complex of associated amino (HCF-1(N))- and carboxy (HCF-1(C))-terminal subunits that result fr...

journal_title：Molecular and cellular biology

authors： Wilson AC,Boutros M,Johnson KM,Herr W

更新日期：2000-09-01 00:00:00

abstract：:TRAIL is an endogenous death receptor ligand also used therapeutically because of its selective proapoptotic activity in cancer cells. In the present study, we examined chromatin alterations induced by TRAIL and show that TRAIL induces a rapid activation of DNA damage response (DDR) pathways with histone H2AX, Chk2, A...

journal_title：Molecular and cellular biology

authors： Solier S,Sordet O,Kohn KW,Pommier Y

更新日期：2009-01-01 00:00:00

abstract：:The 3' ends of metazoan histone mRNAs are generated by specialized processing machinery that cleaves downstream of a conserved stem-loop structure. To examine how this reaction might be influenced by transcription, we used a Drosophila melanogaster in vitro system that supports both processes. In this system the compl...

journal_title：Molecular and cellular biology

authors： Adamson TE,Price DH

更新日期：2003-06-01 00:00:00

abstract：:Studies were performed to verify the physiological significance of attenuation in the life cycle of simian virus 40 and the role of agnoprotein in this process. For these purposes, nuclei were isolated at various times after infection and incubated in vitro in the presence of [alpha-32P]UTP under the standard conditio...

journal_title：Molecular and cellular biology

authors： Hay N,Aloni Y

更新日期：1985-06-01 00:00:00

abstract：:We investigated various parameters associated with the initial selection of mouse 3T6 cells for resistance to single concentrations of methotrexate and characterized resistant colonies for the presence of additional (amplified) copies of the dihydrofolate reductase gene. Our results indicate that the frequency of occu...

journal_title：Molecular and cellular biology

authors： Brown PC,Tlsty TD,Schimke RT

更新日期：1983-06-01 00:00:00

abstract：:Mice lacking neuroD2, a basic helix-loop-helix transcription factor involved in brain development, show growth retardation and other abnormalities consistent with hypothalamic-pituitary-thyroid (HPT) axis dysfunction. neuroD2 is expressed in the paraventricular hypothalamic nuclei, the anterior lobe of pituitary, and ...

journal_title：Molecular and cellular biology

authors： Lin CH,Tapscott SJ,Olson JM

更新日期：2006-06-01 00:00:00