Hypomorphic mutation of the TALE gene Prep1 (pKnox1) causes a major reduction of Pbx and Meis proteins and a pleiotropic embryonic phenotype.

abstract：:The sodium/iodide symporter (NIS) is a plasma membrane protein that mediates active iodide transport in thyroid and mammary cells. It is a prerequisite for radioiodide treatment of thyroid cancer and a promising diagnostic and therapeutic tool for breast cancer. We investigated the molecular mechanisms governing NIS e...

journal_title：Molecular and cellular biology

authors： Dentice M,Luongo C,Elefante A,Romino R,Ambrosio R,Vitale M,Rossi G,Fenzi G,Salvatore D

更新日期：2004-09-01 00:00:00

abstract：:We report the isolation and molecular characterization of the mouse grb2 gene. The product of this gene, the Grb2 protein, is highly related to the Caenorhabditis elegans sem-5 gene product and the human GRB2 protein and displays the same SH3-SH2-SH3 structural motifs. In situ hybridization studies revealed that the m...

journal_title：Molecular and cellular biology

authors： Suen KL,Bustelo XR,Pawson T,Barbacid M

更新日期：1993-09-01 00:00:00

abstract：:Bona fide apoptosis in rat and human leukemia cells, rat thymocytes, and bovine endothelial cells was accompanied by limited and specific cleavage of polysome-associated and monosome-associated 28S rRNA, with 18S rRNA being spared. Specific 28S rRNA cleavage was observed in all instances of apoptotic death accompanied...

journal_title：Molecular and cellular biology

authors： Houge G,Robaye B,Eikhom TS,Golstein J,Mellgren G,Gjertsen BT,Lanotte M,Døskeland SO

更新日期：1995-04-01 00:00:00

abstract：:The nuclear receptor transcription factor, nor-1, is expressed during mammalian development predominantly in the nervous system and is induced in a cell-specific manner in nonneuronal cells in response to a variety of extracellular stimuli. To elucidate the essential developmental functions of this transcription facto...

journal_title：Molecular and cellular biology

authors： Pönniö T,Conneely OM

更新日期：2004-10-01 00:00:00

abstract：:We have analyzed the 5'-flanking region of one of the genes coding for the human acute-phase protein, serum amyloid A (SAA). We found that SAA mRNA could be increased fivefold in transfected cells by treatment with phorbol 12-myristate 13-acetate (PMA). To analyze this observation further, we placed a 265-base-pair 5'...

journal_title：Molecular and cellular biology

authors： Edbrooke MR,Burt DW,Cheshire JK,Woo P

更新日期：1989-05-01 00:00:00

abstract：:Major histocompatibility complex class II (MHCII) molecules play a pivotal role in the immune system because they direct the development and activation of CD4(+) T cells. There are three human MHCII isotypes, HLA-DR, HLA-DQ, and HLA-DP. Key transcription factors controlling MHCII genes have been identified by virtue o...

journal_title：Molecular and cellular biology

authors： Peretti M,Villard J,Barras E,Zufferey M,Reith W

更新日期：2001-09-01 00:00:00

abstract：:The human glycoprotein IIB (GPIIB) gene is expressed only in megakaryocytes, and its promoter displays cell type specificity. We show that this specificity involved two cis-acting sequences. The first one, located at -55, contains a GATA binding site. Point mutations that abolish protein binding on this site decrease ...

journal_title：Molecular and cellular biology

authors： Lemarchandel V,Ghysdael J,Mignotte V,Rahuel C,Roméo PH

更新日期：1993-01-01 00:00:00

abstract：:Gamma interferon activation factor (GAF) rapidly induces transcriptional activation of gamma interferon (IFN-gamma)-responsive genes. Conversion of the GAF from a latent cytoplasmic to an activated, DNA-binding form is an immediate step in the cellular response to IFN-gamma. The amount of IFN-gamma-activated GAF, meas...

journal_title：Molecular and cellular biology

authors： Eilers A,Seegert D,Schindler C,Baccarini M,Decker T

更新日期：1993-06-01 00:00:00

abstract：:Smads 1, 5, and 8 are the intracellular mediators for the bone morphogenetic proteins (BMPs), which play crucial roles during mammalian development. Previous research has shown that Smad1 is important in the formation of the allantois, while Smad5 has been shown to be critical in the process of angiogenesis. To furthe...

journal_title：Molecular and cellular biology

authors： Hester M,Thompson JC,Mills J,Liu Y,El-Hodiri HM,Weinstein M

更新日期：2005-06-01 00:00:00

abstract：:Hox11 is a homeobox gene essential for spleen formation in mice, since atrophy of the anlage of a developing spleen occurs in early embryonic development in Hox11 null mice. HOX11 is also expressed in a subset of T-cell acute leukemias after specific chromosomal translocations. Since the protein has a homeodomain and ...

journal_title：Molecular and cellular biology

authors： Greene WK,Bahn S,Masson N,Rabbitts TH

更新日期：1998-12-01 00:00:00

abstract：:A cDNA encoding a novel Krüppel-type zinc finger protein, FKLF, was cloned from fetal globin-expressing human fetal erythroid cells. The deduced polypeptide sequence composed of 512 amino acids revealed that, like Sp1 and EKLF, FKLF has three contiguous zinc fingers at the near carboxyl-terminal end. A long amino-term...

journal_title：Molecular and cellular biology

authors： Asano H,Li XS,Stamatoyannopoulos G

更新日期：1999-05-01 00:00:00

abstract：:Stp1p and Stp2p are homologous and redundant transcription factors that are synthesized as latent cytoplasmic proteins with N-terminal regulatory domains. In response to extracellular amino acids, the plasma membrane-localized Ssy1p-Ptr3p-Ssy5p (SPS) sensor induces an endoproteolytic processing event that cleaves away...

journal_title：Molecular and cellular biology

authors： Andréasson C,Ljungdahl PO

更新日期：2004-09-01 00:00:00

abstract：:T cell antigen receptor (TCR) activation triggers profound changes in the actin cytoskeleton. In addition to controlling cellular shape and polarity, this process regulates vital T cell responses, such as T cell adhesion, motility, and proliferation. These depend on the recruitment of the signaling proteins Nck and Wi...

journal_title：Molecular and cellular biology

authors： Pauker MH,Reicher B,Fried S,Perl O,Barda-Saad M

更新日期：2011-07-01 00:00:00

abstract：:The HMG box containing protein UBF binds to the promoter of vertebrate ribosomal repeats and is required for their transcription by RNA polymerase I in vitro. UBF can also bind in vitro to a variety of sequences found across the intergenic spacer in Xenopus and mammalian ribosomal DNA (rDNA) repeats. The high abundanc...

journal_title：Molecular and cellular biology

authors： O'Sullivan AC,Sullivan GJ,McStay B

更新日期：2002-01-01 00:00:00

abstract：:We used transformation to insert a selectable marker at various sites in the Saccharomyces cerevisiae genome occupied by the transposable element Ty. The vector CV9 contains the LEU2+ gene and a portion of the repeated element Ty1-17. Transformation with this plasmid resulted in integration of the vector via a recipro...

journal_title：Molecular and cellular biology

authors： Klein HL,Petes TD

更新日期：1984-02-01 00:00:00

abstract：:The MyoD family of basic helix-loop-helix transcription factors function as heterodimers with members of the E-protein family to induce myogenic gene activation. The E-protein HEB is alternatively spliced to generate alpha and beta isoforms. While the function of these molecules has been studied in other cell types, q...

journal_title：Molecular and cellular biology

authors： Parker MH,Perry RL,Fauteux MC,Berkes CA,Rudnicki MA

更新日期：2006-08-01 00:00:00

abstract：:Although TRAIL is considered a potential anticancer agent, it enhances tumor progression by activating NF-κB in apoptosis-resistant cells. Cellular FLICE-like inhibitory protein (cFLIP) overexpression and caspase-8 activation have been implicated in TRAIL-induced NF-κB activation; however, the underlying mechanisms ar...

journal_title：Molecular and cellular biology

authors： Zhang L,Blackwell K,Workman LM,Chen S,Pope MR,Janz S,Habelhah H

更新日期：2015-10-01 00:00:00

abstract：:The message for the zinc finger gene Rex-1 (Zfp-42) is expressed in undifferentiated murine F9 teratocarcinoma cells and embryonic stem cells. Expression of Rex-1 is reduced at the transcriptional level when F9 cells are induced by the addition of retinoic acid (RA) to differentiate. We have isolated genomic DNA for t...

journal_title：Molecular and cellular biology

authors： Hosler BA,Rogers MB,Kozak CA,Gudas LJ

更新日期：1993-05-01 00:00:00

abstract：:In an effort to identify widely active positive regulatory elements, we have examined the action of the cytomegalovirus enhancer-promoter in transgenic mice. These elements activated expression in 24 of 28 tissues tested. The greatest expression was observed in the heart, kidney, brain, and testis. Maximum expression ...

journal_title：Molecular and cellular biology

authors： Schmidt EV,Christoph G,Zeller R,Leder P

更新日期：1990-08-01 00:00:00

abstract：:Ecdysoneless (ECD) is an evolutionarily conserved protein whose germ line deletion is embryonic lethal. Deletion of Ecd in cells causes cell cycle arrest, which is rescued by exogenous ECD, demonstrating a requirement of ECD for normal mammalian cell cycle progression. However, the exact mechanism by which ECD regulat...

journal_title：Molecular and cellular biology

authors： Mir RA,Bele A,Mirza S,Srivastava S,Olou AA,Ammons SA,Kim JH,Gurumurthy CB,Qiu F,Band H,Band V

更新日期：2015-12-28 00:00:00

abstract：:Lacrimo-auriculo-dento-digital (LADD) syndrome is characterized by abnormalities in lacrimal and salivary glands, in teeth, and in the distal limbs. Genetic studies have implicated heterozygous mutations in fibroblast growth factor 10 (FGF10) and in FGF receptor 2 (FGFR2) in LADD syndrome. However, it is not clear whe...

journal_title：Molecular and cellular biology

authors： Shams I,Rohmann E,Eswarakumar VP,Lew ED,Yuzawa S,Wollnik B,Schlessinger J,Lax I

更新日期：2007-10-01 00:00:00

abstract：:In the gal-his3 hybrid promoter his3-GG1, the yeast upstream activator protein GCN4 stimulates transcription when bound at the position normally occupied by the TATA element. This TATA-independent activation by GCN4 requires two additional elements in the gal enhancer region that are distinct from those involved in no...

journal_title：Molecular and cellular biology

authors： Brandl CJ,Struhl K

更新日期：1990-08-01 00:00:00

abstract：:We have partially purified a nuclear protein (PPT) from Physarum polycephalum that binds to the extrachromosomal ribosomal DNA telomeres of this acellular slime mold. Binding is specific for the (T2AG3)n telomere repeats, as evidenced by nitrocellulose filter binding assays, by gel mobility shift assays with both DNA ...

journal_title：Molecular and cellular biology

authors： Coren JS,Epstein EM,Vogt VM

更新日期：1991-04-01 00:00:00

abstract：:A transcriptional enhancer has been mapped to a region 5.5 kilobases 3' of the C beta 2 gene in the human T-cell receptor (TCR) beta-chain locus. Transient transfections allowed localization of enhancer activity to a 480-base-pair HincII-XbaI restriction enzyme fragment. The TCR beta enhancer was active on both the mi...

journal_title：Molecular and cellular biology

authors： Gottschalk LR,Leiden JM

更新日期：1990-10-01 00:00:00

abstract：:Two-dimensional electrophoretograms of newly synthesized polypeptides from low-metastatic (P29) and high-metastatic (D6) Lewis lung carcinoma cells were compared. The results showed that the synthesis of tropomyosin 2 (TM2) was significantly less in D6 cells than in P29 cells. Furthermore, suppression of TM2 synthesis...

journal_title：Molecular and cellular biology

authors： Takenaga K,Nakamura Y,Sakiyama S

更新日期：1988-09-01 00:00:00

abstract：:The chemotropic guidance cue netrin-1 mediates attraction of migrating axons during central nervous system development through the receptor Deleted in Colorectal Cancer (DCC). Downstream of netrin-1, activated Rho GTPases Rac1 and Cdc42 induce cytoskeletal rearrangements within the growth cone. The Rho guanine nucleot...

journal_title：Molecular and cellular biology

authors： DeGeer J,Boudeau J,Schmidt S,Bedford F,Lamarche-Vane N,Debant A

更新日期：2013-02-01 00:00:00

abstract：:Polyoma virus transforms, upon infection or DNA transfection, nonpermissive Fisher rat fibroblasts. Cloned viral DNA was deleted of sequences around the Bg/I site at nucleotide 86 by Bal31 nuclease treatment and then recloned in Escherichia coli. The extent of deletion for each mutant was then determined by DNA sequen...

journal_title：Molecular and cellular biology

authors： Katinka M,Yaniv M

更新日期：1982-10-01 00:00:00

abstract：:We describe here a new cell line, EL2, which spontaneously arose from primary rat embryo fibroblasts and has the distinctive property of being highly susceptible to a number of different transforming genes. The high susceptibility is expressed not only in high transformation frequencies but, most importantly, in an un...

journal_title：Molecular and cellular biology

authors： Liboi E,Caruso M,Basilico C

更新日期：1984-12-01 00:00:00

abstract：:Insults to cellular health cause p53 protein accumulation, and loss of p53 function leads to tumorigenesis. Thus, p53 has to be tightly controlled. Here we report that the BTB/POZ domain transcription factor PATZ1 (MAZR), previously known for its transcriptional suppressor functions in T lymphocytes, is a crucial regu...

journal_title：Molecular and cellular biology

authors： Keskin N,Deniz E,Eryilmaz J,Un M,Batur T,Ersahin T,Cetin Atalay R,Sakaguchi S,Ellmeier W,Erman B

更新日期：2015-05-01 00:00:00

abstract：:The signaling routes linking G-protein-coupled receptors to mitogen-activated protein kinase (MAPK) may involve tyrosine kinases, phosphoinositide 3-kinase gamma (PI3Kgamma), and protein kinase C (PKC). To characterize the mitogenic pathway of bradykinin (BK), COS-7 cells were transiently cotransfected with the human ...

journal_title：Molecular and cellular biology

authors： Adomeit A,Graness A,Gross S,Seedorf K,Wetzker R,Liebmann C

更新日期：1999-08-01 00:00:00