Repression of insulin gene expression by adenovirus type 5 E1a proteins.

abstract：:The biosynthesis, phosphorylation, and degradation of the epidermal growth factor (EGF) receptor were examined in normal human fibroblasts. The receptor was initially synthesized as an Mr = 160,000 immature form which matured to an Mr = 170,000 form in a monensin-sensitive manner. Tunicamycin treatment led to the accu...

journal_title：Molecular and cellular biology

authors： Decker SJ

更新日期：1984-09-01 00:00:00

abstract：:With v-yes DNA as the probe, a human cDNA library made from placental RNA was screened under relaxed conditions, and DNA clones derived from a novel genetic locus, termed lyn, were obtained. Nucleotide sequencing revealed that lyn could encode a novel tyrosine kinase that was very similar to mouse T-lymphocyte-specifi...

journal_title：Molecular and cellular biology

authors： Yamanashi Y,Fukushige S,Semba K,Sukegawa J,Miyajima N,Matsubara K,Yamamoto T,Toyoshima K

更新日期：1987-01-01 00:00:00

abstract：:A cDNA clone which encodes a truncation form of the gamma subtype of the retinoic acid receptor (RAR gamma) has been isolated. The mutant RAR gamma (RAR gamma Bm382) has lost its 65 C-terminal amino acids, thus truncating a part of the dimerization and activation domains. By using a reverse transcription-coupled PCR t...

journal_title：Molecular and cellular biology

authors： Matsui T,Sashihara S

更新日期：1995-04-01 00:00:00

abstract：:CREB binding protein (CBP) is a 270-kDa nuclear protein required for activated transcription of a large number of cellular genes. Although CBP was originally discovered through its interaction with phosphorylated CREB (pCREB), it is utilized by a multitude of cellular transcription factors and viral oncoproteins. Both...

journal_title：Molecular and cellular biology

authors： Giebler HA,Lemasson I,Nyborg JK

更新日期：2000-07-01 00:00:00

abstract：:Amino acids 106 to 143 and 354 to 433 of the human c-myc protein (439 amino acids) were shown to be required for the protein to suppress c-myc gene transcription and were found to exactly overlap with those necessary for c-myc to cooperate with ras oncogenes in the transformation of rat embryo fibroblasts. The essenti...

journal_title：Molecular and cellular biology

authors： Penn LJ,Brooks MW,Laufer EM,Littlewood TD,Morgenstern JP,Evan GI,Lee WM,Land H

更新日期：1990-09-01 00:00:00

abstract：:Mutations in the Saccharomyces cerevisiae alpha-factor receptor that lead to improved response to Saccharomyces kluyveri alpha-factor were identified and sequenced. Mutants were isolated from cells bearing randomly mutagenized receptor gene (STE2) plasmids by an in vivo screen. Five mutations lead to substitutions in ...

journal_title：Molecular and cellular biology

authors： Marsh L

更新日期：1992-09-01 00:00:00

abstract：:TDP-43, or TAR DNA-binding protein 43, is a pathological marker of a spectrum of neurodegenerative disorders, including amyotrophic lateral sclerosis and frontotemporal lobar degeneration with ubiquitin-positive inclusions. TDP-43 is an RNA/DNA-binding protein implicated in transcriptional and posttranscriptional regu...

journal_title：Molecular and cellular biology

authors： Dewey CM,Cenik B,Sephton CF,Dries DR,Mayer P 3rd,Good SK,Johnson BA,Herz J,Yu G

更新日期：2011-03-01 00:00:00

abstract：:Proteins encoded by the mdm2 gene, which has a pivotal role in the regulation of growth and differentiation, exist principally in human and murine cells as two isoforms that migrate in gels as 75-kDa and 90-kDa proteins. There is limited understanding of the respective biological roles of these isoforms, their molecul...

journal_title：Molecular and cellular biology

authors： Cheng TH,Cohen SN

更新日期：2007-01-01 00:00:00

abstract：:We describe a genetic transformation system which should prove useful for investigating tropomyosin assembly and function. Muscle abnormalities associated with a defective tropomyosin allele were corrected by integrating the wild-type gene into germ line chromosomes. The transformation protocol permits application of ...

journal_title：Molecular and cellular biology

authors： Fyrberg EA,Karlik CC

更新日期：1987-08-01 00:00:00

abstract：:The full-length versions of the Ras-specific exchange factors Ras-GRF1 (GRF1) and Ras-GRF2 (GRF2), which are expressed in brain and a restricted number of other organs, possess an ionomycin-dependent activation of Erk mitogen-activated protein kinase activity in 293T cells (C. L. Farnsworth et al., Nature 376:524-527,...

journal_title：Molecular and cellular biology

authors： Anborgh PH,Qian X,Papageorge AG,Vass WC,DeClue JE,Lowy DR

更新日期：1999-07-01 00:00:00

abstract：:Mouse erythroleukemia cells contain several abundant mRNA species that occur to a considerable extent as untranslated molecules. For two of these species, which code for polypeptides P40 and P21, the proportion of molecules engaged in translation decreases rapidly after exposure of the cells to dimethyl sulfoxide. The...

journal_title：Molecular and cellular biology

authors： Yenofsky R,Cereghini S,Krowczynska A,Brawerman G

更新日期：1983-07-01 00:00:00

abstract：:The class II transactivator (CIITA) is induced by gamma interferon (IFN-gamma) and activates major histocompatibility complex class II; however, this report shows it suppresses other genes. An N-terminal 36 amino acids of CIITA mediates suppression of the collagen alpha(2)(I) promoter via binding to CREB-binding prote...

journal_title：Molecular and cellular biology

authors： Zhu XS,Ting JP

更新日期：2001-10-01 00:00:00

abstract：:Mutagenesis studies have demonstrated the requirement for the CD28-responsive element (CD28RE) within the interleukin-2 (IL-2) promoter for transcriptional upregulation by CD28. Here, we demonstrate that CD28 responsiveness is conferred by a composite element containing both the CD28RE and the NF-IL-2B AP-1 sites (RE/...

journal_title：Molecular and cellular biology

authors： Shapiro VS,Truitt KE,Imboden JB,Weiss A

更新日期：1997-07-01 00:00:00

abstract：:The proteins encoded by both viral and cellular forms of the c-myc oncogene have been previously demonstrated to have exceptionally short in vivo half-lives. In this paper we report a comparative study on the parameters affecting turnover of nuclear oncoproteins c-myc, c-myb, and the rapidly metabolized cytoplasmic en...

journal_title：Molecular and cellular biology

authors： Lüscher B,Eisenman RN

更新日期：1988-06-01 00:00:00

abstract：:A C/EBP-like transcription factor, AGP/EBP, that binds to three distinct motifs in the 5'-flanking region of alpha 1-acid glycoprotein gene (AGP) has been identified. Here we report the cloning and properties of cDNA corresponding to mouse AGP/EBP. AGP/EBP and C/EBP share 87% amino acid sequence homology in the "leuci...

journal_title：Molecular and cellular biology

authors： Chang CJ,Chen TT,Lei HY,Chen DS,Lee SC

更新日期：1990-12-01 00:00:00

abstract：:DET1 (de-etiolated 1) is an essential negative regulator of plant light responses, and it is a component of the Arabidopsis thaliana CDD complex containing DDB1 and COP10 ubiquitin E2 variant. Human DET1 has recently been isolated as one of the DDB1- and Cul4A-associated factors, along with an array of WD40-containing...

journal_title：Molecular and cellular biology

authors： Pick E,Lau OS,Tsuge T,Menon S,Tong Y,Dohmae N,Plafker SM,Deng XW,Wei N

更新日期：2007-07-01 00:00:00

abstract：:Eucaryotic initiation factor 4A (eIF-4A) is a member of a family of proteins believed to be involved in the ATP-dependent melting of RNA secondary structure. These proteins contain a derivative of the consensus ATP-binding site AXXGXGKT. To assess the importance of the consensus amino acid sequence in eIF-4A for ATP b...

journal_title：Molecular and cellular biology

authors： Rozen F,Pelletier J,Trachsel H,Sonenberg N

更新日期：1989-09-01 00:00:00

abstract：:The regulation of telomerase reverse transcriptase (TERT) plays an important role in the proliferative capacity and survival of cells. Here, we report that exogenously as well as endogenously induced oxidative stress leads to translocation of endogenous as well as overexpressed human TERT from the nucleus into the cyt...

journal_title：Molecular and cellular biology

authors： Haendeler J,Hoffmann J,Brandes RP,Zeiher AM,Dimmeler S

更新日期：2003-07-01 00:00:00

abstract：:We have investigated the site of surface expression of the neuraminidase (NA) glycoprotein of influenza A virus, which, in contrast to the hemagglutinin, is bound to membranes by hydrophobic residues near the NH2-terminus. Madin-Darby canine kidney or primary African green monkey kidney cells infected with influenza A...

journal_title：Molecular and cellular biology

authors： Jones LV,Compans RW,Davis AR,Bos TJ,Nayak DP

更新日期：1985-09-01 00:00:00

abstract：:The uptake of various nucleosides by S49 mouse T-lymphoma cells and that by a single-step nucleoside transport-defective mutant thereof (AE1) were compared. Residual nucleoside entry into AE1 cells occurred via two routes, nonmediated permeation and saturable, non-concentrative transport with broad substrate specifici...

journal_title：Molecular and cellular biology

authors： Plagemann PG,Woffendin C

更新日期：1987-01-01 00:00:00

abstract：:The Wnt-1 proto-oncogene is essential for proper development of the midbrain and is expressed in a spatially and temporally restricted manner during central nervous system development in mice. In vitro, the gene is specifically transcribed during the retinoic acid (RA)-induced neuroectodermal differentiation of the P1...

journal_title：Molecular and cellular biology

authors： St-Arnaud R,Moir JM

更新日期：1993-03-01 00:00:00

abstract：:The retinoblastoma tumor suppressor protein (Rb) plays a vital role in regulating mammalian cell cycle progression and inactivation of Rb is necessary for entry into S phase. Rb is inactivated by phosphorylation upon growth factor stimulation of quiescent cells, facilitating the transition from G(1) phase to S phase. ...

journal_title：Molecular and cellular biology

authors： Dasgupta P,Sun J,Wang S,Fusaro G,Betts V,Padmanabhan J,Sebti SM,Chellappan SP

更新日期：2004-11-01 00:00:00

abstract：:An enzyme that hydrolyzes one PPi bond of the cap structure of mRNA, yielding m7GDP and 5'-p RNA was purified from Saccharomyces cerevisiae to a stage suitable for characterization. The specificity of the enzyme was studied, using both yeast mRNA and synthetic RNAs labeled in the cap structure. A synthetic capped RNA ...

journal_title：Molecular and cellular biology

authors： Stevens A

更新日期：1988-05-01 00:00:00

abstract：:p27(Kip1) is a member of the Cip-Kip family of cyclin-dependent kinase (Cdk) inhibitors that binds to cyclin-Cdk complexes and inhibits their catalytic activity in response to antiproliferative stimuli. p27(Kip1) is regulated by several posttranscriptional mechanisms, including subcellular localization. We have identi...

journal_title：Molecular and cellular biology

authors： Smitherman M,Lee K,Swanger J,Kapur R,Clurman BE

更新日期：2000-08-01 00:00:00

abstract：:Histone H1 is an abundant and essential component of chromatin whose precise role in regulating gene expression is poorly understood. Here, we report that a major target of H1-mediated regulation in embryonic stem (ES) cells is the X-linked Rhox homeobox gene cluster. To address the underlying mechanism, we examined t...

journal_title：Molecular and cellular biology

authors： Maclean JA,Bettegowda A,Kim BJ,Lou CH,Yang SM,Bhardwaj A,Shanker S,Hu Z,Fan Y,Eckardt S,McLaughlin KJ,Skoultchi AI,Wilkinson MF

更新日期：2011-03-01 00:00:00

abstract：:Hydroxyurea (HU) is a DNA replication inhibitor that negatively affects both the elongation and initiation phases of replication and triggers the "intra-S phase checkpoint." Previous work with budding yeast has shown that, during a short exposure to HU, MEC1/RAD53 prevent initiation at some late S phase origins. In th...

journal_title：Molecular and cellular biology

authors： Alvino GM,Collingwood D,Murphy JM,Delrow J,Brewer BJ,Raghuraman MK

更新日期：2007-09-01 00:00:00

abstract：:A series of extraction procedures were applied to avian nuclei which allowed us to define three types of association of v-myc- and c-myc-encoded proteins with nuclei: (i) a major fraction (60 to 90%) which is retained in DNA- and RNA-depleted nuclei after low- and high-salt extraction, (ii) a small fraction (1%) relea...

journal_title：Molecular and cellular biology

authors： Eisenman RN,Tachibana CY,Abrams HD,Hann SR

更新日期：1985-01-01 00:00:00

abstract：:To identify novel genes that play critical roles in mediating bone morphogenetic protein (BMP) signal pathways, we performed a yeast two-hybrid screen using Smad1 as bait. A novel mouse Krüppel-type zinc finger protein, mZnf8, was isolated. Interactions between mZnf8 and Smad proteins were further analyzed with variou...

journal_title：Molecular and cellular biology

authors： Jiao K,Zhou Y,Hogan BL

更新日期：2002-11-01 00:00:00

abstract：:An increased level of unpolymerized tubulin caused by depolymerization of microtubules in sea urchin larvae resulted in a rapid loss of tubulin mRNA, which was prevented by nearly complete inhibition of protein synthesis. Results of an RNA run-on assay indicated that inhibition of protein synthesis does not alter tubu...

journal_title：Molecular and cellular biology

authors： Gong ZY,Brandhorst BP

更新日期：1988-08-01 00:00:00

abstract：:Src homology 2 (SH2) domains provide specificity to intracellular signaling by binding to specific phosphotyrosine (phospho-Tyr)-containing sequences. We recently developed a technique using a degenerate phosphopeptide library to predict the specificity of individual SH2 domains (src family members, Abl, Nck, Sem5, ph...

journal_title：Molecular and cellular biology

authors： Songyang Z,Shoelson SE,McGlade J,Olivier P,Pawson T,Bustelo XR,Barbacid M,Sabe H,Hanafusa H,Yi T

更新日期：1994-04-01 00:00:00