Abstract:
:The enhancer of yellow 1 gene, e(y)1, of Drosophila melanogaster has been cloned and demonstrated to encode the TAFII40 protein. The e(y)1 gene is expressed in females much more strongly than in males due to the accumulation of e(y)1 mRNA in the ovaries. Two different e(y)1 mutations have been obtained. The e(y)1(ul) mutation, induced by the insertion of Stalker into the coding region, leads to the replacement of 25 carboxy-terminal amino acids by 17 amino acids encoded by the Stalker sequences and to a decrease of the e(y)1 transcription level. The latter is the main cause of dramatic underdevelopment of the ovaries and sterility of females bearing the e(y)1 mutation. This follows from the restoration of female fertility upon transformation of e(y)1(u1) flies with a construction synthesizing the mutant protein. The e(y)1(P1) mutation induced by P element insertion into the transcribed nontranslated region of the gene has almost no influence on the phenotype of flies. However, in combination with the phP1 mutation, which leads to a strong P element-mediated suppression of e(y)1 transcription, this mutation is lethal. Genetic studies of the e(y)1(u1) mutation revealed a sensitivity of the yellow and white expression to the TAFII40/e(y)1 level. The su(Hw)-binding region, Drosophila insulator, stabilizes the expression of the white gene and makes it independent of the e(y)1(u1) mutation.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Soldatov A,Nabirochkina E,Georgieva S,Belenkaja T,Georgiev Pdoi
10.1128/mcb.19.5.3769subject
Has Abstractpub_date
1999-05-01 00:00:00pages
3769-78issue
5eissn
0270-7306issn
1098-5549journal_volume
19pub_type
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