Abstract:
:We have studied the mechanism by which 5'-flanking sequences modulate the in vitro transcription of eucaryotic tRNA genes. Using deletion and linker substitution mutagenesis, we have found that the 5'-flanking sequences responsible for the different in vitro transcription levels of three Drosophila tRNA5Asn genes are contained within a discrete region centered 22 nucleotides upstream from the transcription initiation site. In conjunction with the A-box intragenic control region, this upstream transcription-modulatory region functions in the selection mechanism for the site of transcription initiation. Since the transcription-modulatory region directs the position of the start site and the actual sequence of the transcription-modulatory region determines the level of tRNAAsn gene transcription, the possibility is raised that the transcription-modulatory region directs a transcription initiation event similar to open complex formation at procaryotic promoters.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Lofquist AK,Garcia AD,Sharp SJdoi
10.1128/mcb.8.10.4441subject
Has Abstractpub_date
1988-10-01 00:00:00pages
4441-9issue
10eissn
0270-7306issn
1098-5549journal_volume
8pub_type
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