Proteomics of the eukaryotic transcription machinery: identification of proteins associated with components of yeast TFIID by multidimensional mass spectrometry.

abstract：:This paper presents a general means of eliminating the function of a single protein without relying on genetic alterations in its structure or level of synthesis. The strategy is based on the inducible cellular expression of neutralizing antibody to inactivate the protein selectively. The feasibility of this approach ...

journal_title：Molecular and cellular biology

authors： Carlson JR

更新日期：1988-06-01 00:00:00

abstract：:In mast cells, antigen-mediated aggregation of the high-affinity receptor for immunoglobulin E, Fc epsilon RI, stimulates tyrosine phosphorylation and activation of multiple signaling pathways leading to the release of several classes of mediators of the allergic response. Early events induced upon cross-linking of Fc...

journal_title：Molecular and cellular biology

authors： Rivera VM,Brugge JS

更新日期：1995-03-01 00:00:00

abstract：:Miz1 is a member of the POZ domain/zinc finger transcription factor family. In vivo, Miz1 forms a complex with the Myc oncoprotein and recruits Myc to core promoter elements. Myc represses transcription through Miz1 binding sites. We now show that the Miz1 gene is ubiquitously expressed during mouse embryogenesis. In ...

journal_title：Molecular and cellular biology

authors： Adhikary S,Peukert K,Karsunky H,Beuger V,Lutz W,Elsässer HP,Möröy T,Eilers M

更新日期：2003-11-01 00:00:00

abstract：:The cytoplasm of Saccharomyces cerevisiae contains two major classes of protein-encapsulated double-stranded ribonucleic acids (dsRNA's), L and M. Replication of L and M dsRNA's was examined in cells arrested in the G1 phase by either alpha-factor, a yeast mating pheromone, or the restrictive temperature for a cell cy...

journal_title：Molecular and cellular biology

authors： Zakian VA,Wagner DW,Fangman WL

更新日期：1981-08-01 00:00:00

abstract：:Chicken heart mesenchymal cells do not proliferate in medium of physiological composition containing plasma (S. Balk, Proc. Natl. Acad. Sci. USA 77:6606-6610, 1980). To understand the molecular events involved in cell quiescence and in the initiation of cell division under physiological conditions, we examined the dif...

journal_title：Molecular and cellular biology

authors： Bédard PA,Balk SD,Gunther HS,Morisi A,Erikson RL

更新日期：1987-04-01 00:00:00

abstract：:Upon the addition of different growth factors and cytokines, the Gab1 docking protein is tyrosine phosphorylated and in turn activates different signaling pathways. On the basis of the large body of evidence concerning cross talk between the signaling pathways activated by growth factors and oxidative stress, we decid...

journal_title：Molecular and cellular biology

authors： Holgado-Madruga M,Wong AJ

更新日期：2003-07-01 00:00:00

abstract：:In Caenorhabditis elegans, the central cell-killing process is essentially controlled by the interplay of four apoptotic factors: EGL-1/BH3-only protein, CED-9/Bcl2, CED-4/Apaf1, and CED-3/caspase. In cells destined to die, EGL-1 binds to CED-9 and results in the release of CED-4 from the mitochondrion-tethered CED-9-...

journal_title：Molecular and cellular biology

authors： Shen Q,Qin F,Gao Z,Cui J,Xiao H,Xu Z,Yang C

更新日期：2009-07-01 00:00:00

abstract：:The distal region (-830 to -720 bp) of the rat whey acidic protein (WAP) gene contains a composite response element (CoRE), which has been demonstrated previously to confer mammary gland-specific and hormonally regulated WAP gene expression. Point mutations in the binding sites for specific transcription factors prese...

journal_title：Molecular and cellular biology

authors： Mukhopadhyay SS,Wyszomierski SL,Gronostajski RM,Rosen JM

更新日期：2001-10-01 00:00:00

abstract：:The activation of p70s6k is associated with multiple phosphorylations at two sets of sites. The first set, S411, S418, T421, and S424, reside within the autoinhibitory domain, and each contains a hydrophobic residue at -2 and a proline at +1. The second set of sites, T229 (in the catalytic domain) and T389 and S404 (i...

journal_title：Molecular and cellular biology

authors： Moser BA,Dennis PB,Pullen N,Pearson RB,Williamson NA,Wettenhall RE,Kozma SC,Thomas G

更新日期：1997-09-01 00:00:00

abstract：:Type I interferons (IFNs) stimulate transcription through a latent heterotrimeric transcription factor composed of tyrosine-phosphorylated STAT1 and STAT2 and the DNA binding partner IRF9, with STAT2 contributing a critical transactivation domain. Human RVB1 and RVB2, which are highly conserved AAA(+) ATP binding prot...

journal_title：Molecular and cellular biology

authors： Gnatovskiy L,Mita P,Levy DE

更新日期：2013-10-01 00:00:00

abstract：:The Id subfamily of helix-loop-helix (HLH) proteins plays a fundamental role in the regulation of cellular proliferation and differentiation. The major mechanism by which Id proteins are thought to inhibit differentiation is through interaction with other HLH proteins and inhibition of their DNA-binding activity. Howe...

journal_title：Molecular and cellular biology

authors： Roberts EC,Deed RW,Inoue T,Norton JD,Sharrocks AD

更新日期：2001-01-01 00:00:00

abstract：:The lactogenic hormones, i.e., prolactin and glucocorticoids, act in concert to stimulate transcription factors responsible for hormone-dependent milk protein gene expression. In the mammary gland, prolactin activates Stat5a and Stat5b and glucocorticoids activate the glucocorticoid receptor (GR). Immunoprecipitation ...

journal_title：Molecular and cellular biology

authors： Cella N,Groner B,Hynes NE

更新日期：1998-04-01 00:00:00

abstract：:Multiple members of the A, B, and C clusters of Hox genes are expressed in hematopoietic cells. Several of these Hox genes have been found to display distinctive expression patterns, with genes located at the 3' side of the clusters being expressed at their highest levels in the most primitive subpopulation of human C...

journal_title：Molecular and cellular biology

authors： Thorsteinsdottir U,Sauvageau G,Hough MR,Dragowska W,Lansdorp PM,Lawrence HJ,Largman C,Humphries RK

更新日期：1997-01-01 00:00:00

abstract：:To distinguish among possible mechanisms of repair of a double-strand break (DSB) by gene conversion in budding yeast, Saccharomyces cerevisiae, we employed isotope density transfer to analyze budding yeast mating type (MAT) gene switching in G2/M-arrested cells. Both of the newly synthesized DNA strands created durin...

journal_title：Molecular and cellular biology

authors： Ira G,Satory D,Haber JE

更新日期：2006-12-01 00:00:00

abstract：:Signal transducers and activators of transcription (STATs) are transcription factors that mediate normal biologic responses to cytokines and growth factors. However, abnormal activation of certain STAT family members, including Stat3, is increasingly associated with oncogenesis. In fibroblasts expressing the Src oncop...

journal_title：Molecular and cellular biology

authors： Turkson J,Bowman T,Adnane J,Zhang Y,Djeu JY,Sekharam M,Frank DA,Holzman LB,Wu J,Sebti S,Jove R

更新日期：1999-11-01 00:00:00

abstract：:The "pocket" proteins pRb, p107, and p130 are a family of negative growth regulators. Previous studies have demonstrated that overexpression of pRb can repress transcription by RNA polymerase (Pol) I. To assess whether pRb performs this role under physiological conditions, we have examined pre-rRNA levels in cells fro...

journal_title：Molecular and cellular biology

authors： Ciarmatori S,Scott PH,Sutcliffe JE,McLees A,Alzuherri HM,Dannenberg JH,te Riele H,Grummt I,Voit R,White RJ

更新日期：2001-09-01 00:00:00

abstract：:Introduction of simian virus 40 T antigen into rodent fibroblasts gives rise to cells that can proliferate indefinitely but are dependent upon it for maintenance of their growth once the normal mitotic life span has elapsed. Inactivation of T antigen in these immortalized cells causes rapid and irreversible cessation ...

journal_title：Molecular and cellular biology

authors： Gonos ES,Burns JS,Mazars GR,Kobrna A,Riley TE,Barnett SC,Zafarana G,Ludwig RL,Ikram Z,Powell AJ,Jat PS

更新日期：1996-09-01 00:00:00

abstract：:In resting cells, the NFAT1 transcription factor is kept inactive in the cytoplasm by phosphorylation on multiple serine residues. These phosphorylated residues are primarily contained within two types of serine-rich motifs, the SRR-1 and SP motifs, which are conserved within the NFAT family. Several different kinases...

journal_title：Molecular and cellular biology

authors： Okamura H,Garcia-Rodriguez C,Martinson H,Qin J,Virshup DM,Rao A

更新日期：2004-05-01 00:00:00

abstract：:Barsoum and Varshavsky (Proc. Natl. Acad. Sci. U.S.A. 80:5330-5334, 1983) suggest that polypeptide mitogens and the mitogenic tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) stimulate gene amplification by related pathways. I demonstrated that TPA and the polypeptide mitogen fibroblast growth factor (FGF) bo...

journal_title：Molecular and cellular biology

authors： Herschman HR

更新日期：1985-05-01 00:00:00

abstract：:Retinoid X receptors (RXR) have been identified as common subunits in the regulation of multiple hormonal signaling pathways. Using circular permutation and phasing analysis of specific response elements, we present evidence that RXR-retinoic acid receptor and RXR-thyroid hormone receptor heterodimer or RXR-RXR homodi...

journal_title：Molecular and cellular biology

authors： Lu XP,Eberhardt NL,Pfahl M

更新日期：1993-10-01 00:00:00

abstract：:The genes CDC36, CDC37, and CDC39, thought to function in the cell division control process in Saccharomyces cerevisiae, were isolated from a recombinant plasmid library prepared by partial digestion of S. cerevisiae genomic DNA with Sau3A and insertion into the S. cerevisiae-Escherichia coli shuttle vector YRp7. In e...

journal_title：Molecular and cellular biology

authors： Breter HJ,Ferguson J,Peterson TA,Reed SI

更新日期：1983-05-01 00:00:00

abstract：:Regulatory regions of the mouse muscle creatine kinase (MCK) gene, previously discovered by analysis in cultured muscle cells, were analyzed in transgenic mice. The 206-bp MCK enhancer at nt-1256 was required for high-level expression of MCK-chloramphenicol acetyltransferase fusion genes in skeletal and cardiac muscle...

journal_title：Molecular and cellular biology

authors： Donoviel DB,Shield MA,Buskin JN,Haugen HS,Clegg CH,Hauschka SD

更新日期：1996-04-01 00:00:00

abstract：:KIF14 is a microtubule motor protein whose elevated expression is associated with poor-prognosis breast cancer. Here we demonstrate KIF14 accumulation in mitotic cells, where it associated with developing spindle poles and spindle microtubules. Cells at later stages of mitosis were characterized by the concentration o...

journal_title：Molecular and cellular biology

authors： Carleton M,Mao M,Biery M,Warrener P,Kim S,Buser C,Marshall CG,Fernandes C,Annis J,Linsley PS

更新日期：2006-05-01 00:00:00

abstract：:Sequences of DNA that hybridize on Southern blots with cloned intracisternal A-particle (IAP) sequences have been examined in genomic DNAs of neonatal mice, livers of adult mice (3, 6, 12, 18, 24, and 26 months old), and the solid myeloma tumor MOPC-315. The isoschizomers HpaII (CCGG or mCCGG) and MspI (CCGG or CmCGG)...

journal_title：Molecular and cellular biology

authors： Mays-Hoopes LL,Brown A,Huang RC

更新日期：1983-08-01 00:00:00

abstract：:At least two separate but interdependent events are required to attain autonomous growth as a consequence of ectopic expression of the multilineage colony-stimulating factor gene in hematopoietic progenitor cells. The rate at which the second event occurs is more than 3 orders of magnitude higher in precursor cell lin...

journal_title：Molecular and cellular biology

authors： Laker C,Kluge N,Stocking C,Just U,Franz MJ,Ostertag W,DeLamarter JF,Dexter M,Spooncer E

更新日期：1989-12-01 00:00:00

abstract：:Insulin-like growth factor II (IGF-II) is a major fetal growth factor. The IGF-II gene generates multiple mRNAs with different 5' untranslated regions (5' UTRs) that are translated in a differential manner during development. We have identified a human family of three IGF-II mRNA-binding proteins (IMPs) that exhibit m...

journal_title：Molecular and cellular biology

authors： Nielsen J,Christiansen J,Lykke-Andersen J,Johnsen AH,Wewer UM,Nielsen FC

更新日期：1999-02-01 00:00:00

abstract：:pp60c-src was localized in the cerebellum of developing chicken embryos by immunoperoxidase staining with antisera raised against bacterially expressed pp60v-src. Immunoreactivity (IR) appeared in the cerebellum of the chicken embryos at the time of neuronal differentiation. pp60c-src IR was detected in regions of the...

journal_title：Molecular and cellular biology

authors： Fults DW,Towle AC,Lauder JM,Maness PF

更新日期：1985-01-01 00:00:00

abstract：:Cellular senescence is a tumor-suppressive process characterized by an irreversible cell cycle exit, a unique morphology, and expression of senescence-associated beta-galactosidase (SA-beta-Gal). We report here a role for CDK5 in induction of senescent cytoskeletal changes. CDK5 activation is upregulated in senescing ...

journal_title：Molecular and cellular biology

authors： Alexander K,Yang HS,Hinds PW

更新日期：2004-04-01 00:00:00

abstract：:To investigate the role of myogenin in regulating acetylcholine receptor expression in adult muscle, this muscle-specific basic helix-loop-helix transcription factor was overexpressed in transgenic mice by using regulatory elements conferring strong expression confined to differentiated postmitotic muscle fibers. Many...

journal_title：Molecular and cellular biology

authors： Gundersen K,Rabben I,Klocke BJ,Merlie JP

更新日期：1995-12-01 00:00:00

abstract：:We examined the effects of 18 h of incubation of Chinese hamster ovary (CHO K1) cells with cycloheximide, hydroxyurea, and aphidicolin. Treatment of cells with cycloheximide alone at a concentration adequate to inhibit DNA synthesis to less than 10% of control was significantly less cytotoxic and clastogenic than trea...

journal_title：Molecular and cellular biology

authors： Sherwood SW,Schumacher RI,Schimke RT

更新日期：1988-07-01 00:00:00