Abstract:
:Miz1 is a member of the POZ domain/zinc finger transcription factor family. In vivo, Miz1 forms a complex with the Myc oncoprotein and recruits Myc to core promoter elements. Myc represses transcription through Miz1 binding sites. We now show that the Miz1 gene is ubiquitously expressed during mouse embryogenesis. In order to elucidate the physiological function of Miz1, we have deleted the mouse Miz1 gene by homologous recombination. Miz1(+/-) mice are indistinguishable from wild-type animals; in contrast, Miz1(-/-) embryos are not viable. They are severely retarded in early embryonic development and do not undergo normal gastrulation. Expression of Goosecoid and Brachyury is detectable in Miz1(-/-) embryos, suggesting that Miz1 is not required for signal transduction by Nodal. Expression of p21Cip1, a target gene of Miz1 is unaltered; in contrast, expression of p57Kip2, another target gene of Miz1 is absent in Miz1(-/-) embryos. Miz1(-/-) embryos succumb to massive apoptosis of ectodermal cells around day 7.5 of embryonic development. Our results show that Miz1 is required for early embryonic development during gastrulation.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Adhikary S,Peukert K,Karsunky H,Beuger V,Lutz W,Elsässer HP,Möröy T,Eilers Mdoi
10.1128/mcb.23.21.7648-7657.2003subject
Has Abstractpub_date
2003-11-01 00:00:00pages
7648-57issue
21eissn
0270-7306issn
1098-5549journal_volume
23pub_type
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