Abstract:
:Although DNA breakage and reunion in nonhomologous recombination are poorly understood, previous work suggests that short sequence homologies may play a role in the end-joining step in mammalian cells. To study the mechanism of end joining in more detail, we inserted a polylinker into the simian virus 40 T-antigen intron, cleaved the polylinker with different pairs of restriction enzymes, and transfected the resulting linear molecules into monkey cells. Analysis of 199 independent junctional sequences from seven constructs with different mismatched ends indicates that single-stranded extensions are relatively stable in monkey cells and that the terminal few nucleotides are critical for cell-mediated end joining. Furthermore, these studies define three mechanisms for end joining: single-strand, template-directed, and postrepair ligations. The latter two mechanisms depend on homologous pairing of one to six complementary bases to position the junction. All three mechanisms operate with similar overall efficiencies. The relevance of this work to targeted integration in mammalian cells is discussed.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Roth DB,Wilson JHdoi
10.1128/mcb.6.12.4295subject
Has Abstractpub_date
1986-12-01 00:00:00pages
4295-304issue
12eissn
0270-7306issn
1098-5549journal_volume
6pub_type
杂志文章abstract::The mitogen-activated protein (MAP) kinases represent one of the most important classes of signaling cascades that are used by eukaryotic cells to sense extracellular signals. One of the major responses to these cascades is a change in cellular gene expression profiles mediated through the direct targeting of transcri...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.02276-06
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.13.11.6752
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:1996-05-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.18.12.7139
更新日期:1998-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/mcb.12.10.4271
更新日期:1992-10-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.25.6.2486-2497.2005
更新日期:2005-03-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.5.5.1015
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2005-07-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.16.7.3255
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pub_type: 杂志文章
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更新日期:2003-03-01 00:00:00
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pub_type: 杂志文章
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journal_title:Molecular and cellular biology
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更新日期:1986-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1982-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-09-01 00:00:00