Rev7/MAD2B links c-Jun N-terminal protein kinase pathway signaling to activation of the transcription factor Elk-1.

abstract：:Scaffold proteins have been established as important mediators of signal transduction specificity. The insulin receptor substrate (IRS) proteins represent a critical group of scaffold proteins that are required for signal transduction by the insulin receptor, including the activation of phosphatidylinositol 3 kinase. ...

journal_title：Molecular and cellular biology

authors： Standen CL,Kennedy NJ,Flavell RA,Davis RJ

更新日期：2009-09-01 00:00:00

abstract：:We have studied the patterns of expression of four B-type cyclins (Clbs), Clb1, Clb2, Clb3, and Clb4, and their ability to activate p34cdc28 during the mitotic and meiotic cell cycles of Saccharomyces cerevisiae. During the mitotic cell cycle, Clb3 and Clb4 were expressed and induced a kinase activity in association w...

journal_title：Molecular and cellular biology

authors： Grandin N,Reed SI

更新日期：1993-04-01 00:00:00

abstract：:Fibroblast cultures prepared from mice homozygous for a Robertsonian translocation (centric fusion) between autosomes 8 and 17 [Rb(8.17)] were used as donors in microcell-mediated chromosome transfer experiments. By using hamster recipient cells deficient in adenine phosphoribosyltransferase (APRT-) and selecting for ...

journal_title：Molecular and cellular biology

authors： Fournier RE,Frelinger JA

更新日期：1982-05-01 00:00:00

abstract：:The Cyp cell line consists of mouse cells transformed by a thermosensitive polyomavirus (Py) genome and routinely propagated at 39 degrees C. Cyp cells are readily induced to synthesize free Py DNA by being transferred to 33 degrees C. In one subclone (C12/a1/S48, or S48) of this line, such induction resulted in the i...

journal_title：Molecular and cellular biology

authors： Huberdeau D,Sylla BS,Herring-Gillam E,Bourgaux-Ramoisy D,Bourgaux P

更新日期：1985-10-01 00:00:00

abstract：:Somatic cell nuclear cloning has repeatedly demonstrated striking reversibility of epigenetic regulation of cell differentiation. Upon injection into eggs, the donor nuclei exhibit global chromatin decondensation, which might contribute to reprogramming the nuclei by derepressing dormant genes. Decondensation of sperm...

journal_title：Molecular and cellular biology

authors： Tamada H,Van Thuan N,Reed P,Nelson D,Katoku-Kikyo N,Wudel J,Wakayama T,Kikyo N

更新日期：2006-02-01 00:00:00

abstract：:A cell-free nuclear replication system that is S-phase specific, that requires the activity of DNA polymerase alpha, and that is stimulated three- to eightfold by cytoplasmic factors from S-phase cells was used to examine the temporal specificity of chromosomal DNA synthesis in vitro. Temporal specificity of DNA synth...

journal_title：Molecular and cellular biology

authors： Heintz NH,Stillman BW

更新日期：1988-05-01 00:00:00

abstract：:Gap junctions are membrane channels that permit the interchange of ions and other low-molecular-weight molecules between adjacent cells. Rous sarcoma virus (RSV)-induced transformation is marked by an early and profound disruption of gap-junctional communication, suggesting that these membrane structures may serve as ...

journal_title：Molecular and cellular biology

authors： Crow DS,Beyer EC,Paul DL,Kobe SS,Lau AF

更新日期：1990-04-01 00:00:00

abstract：:We constructed a series of recombinant genomes which directed expression of the enzyme chloramphenicol acetyltransferase (CAT) in mammalian cells. The prototype recombinant in this series, pSV2-cat, consisted of the beta-lactamase gene and origin of replication from pBR322 coupled to a simian virus 40 (SV40) early tra...

journal_title：Molecular and cellular biology

authors： Gorman CM,Moffat LF,Howard BH

更新日期：1982-09-01 00:00:00

abstract：:Precise regulation of cellular proliferation, differentiation, and senescence results in the continuous renewal of the intestinal epithelium with maintenance of a highly ordered tissue architecture. Here we show that an intestine-specific homeobox gene, Cdx2, is a transcription factor that regulates both proliferation...

journal_title：Molecular and cellular biology

authors： Suh E,Traber PG

更新日期：1996-02-01 00:00:00

abstract：:A key antioxidant pathway, the Keap1-Nrf2 system, is regulated by p62/Sqstm1 via multiple mechanisms, including gene expression, posttranslational modifications (such as ubiquitination and phosphorylation), and autophagic degradation of p62/Sqstm1 and Keap1. Here we demonstrate a novel mode of regulation of the Keap1-...

journal_title：Molecular and cellular biology

authors： Kageyama S,Saito T,Obata M,Koide RH,Ichimura Y,Komatsu M

更新日期：2018-03-15 00:00:00

abstract：:The gene encoding the SNF5/Ini1 core subunit of the SWI/SNF chromatin remodeling complex is a tumor suppressor in humans and mice, with an essential role in early embryonic development. To investigate further the function of this gene, we have generated a Cre/lox-conditional mouse line. We demonstrate that Snf5 deleti...

journal_title：Molecular and cellular biology

authors： Klochendler-Yeivin A,Picarsky E,Yaniv M

更新日期：2006-04-01 00:00:00

abstract：:The Integrator Complex is a group of proteins responsible for the endonucleolytic cleavage of primary small nuclear RNA (snRNA) transcripts within the nucleus. Integrator subunits 9 and 11 (IntS9/11) are thought to contain the catalytic activity based on their high sequence similarity to CPSF100 and CPSF73, which have...

journal_title：Molecular and cellular biology

authors： Albrecht TR,Wagner EJ

更新日期：2012-03-01 00:00:00

abstract：:The full-length versions of the Ras-specific exchange factors Ras-GRF1 (GRF1) and Ras-GRF2 (GRF2), which are expressed in brain and a restricted number of other organs, possess an ionomycin-dependent activation of Erk mitogen-activated protein kinase activity in 293T cells (C. L. Farnsworth et al., Nature 376:524-527,...

journal_title：Molecular and cellular biology

authors： Anborgh PH,Qian X,Papageorge AG,Vass WC,DeClue JE,Lowy DR

更新日期：1999-07-01 00:00:00

abstract：:Deregulated signaling by the four members of the epidermal growth factor receptor tyrosine kinase family (erbB family) is implicated in the genesis or progression of human cancers. However, efforts to analyze signaling by these receptors have been hampered by the diversity of ligands and extensive interreceptor cross ...

journal_title：Molecular and cellular biology

authors： Riese DJ 2nd,van Raaij TM,Plowman GD,Andrews GC,Stern DF

更新日期：1995-10-01 00:00:00

abstract：:The intergenic spacer region of the Xenopus laevis ribosomal DNA contains multiple elements which are either 60 or 81 base pairs long. Clusters of these elements have previously been shown to act as position- and distance-independent enhancers on an RNA polymerase I promoter when located in cis. By a combination of de...

journal_title：Molecular and cellular biology

authors： Pikaard CS,Reeder RH

更新日期：1988-10-01 00:00:00

abstract：:Transcriptome profiling studies have recently uncovered a large number of noncoding RNA transcripts (ncRNAs) in eukaryotic organisms, and there is growing interest in their role in the cell. For example, in haploid Saccharomyces cerevisiae cells, the expression of an overlapping antisense ncRNA, referred to here as RM...

journal_title：Molecular and cellular biology

authors： Gelfand B,Mead J,Bruning A,Apostolopoulos N,Tadigotla V,Nagaraj V,Sengupta AM,Vershon AK

更新日期：2011-04-01 00:00:00

abstract：:Chondromodulin I (chm-I), a type II transmembrane protein, is highly expressed in the avascular zones of cartilage but is downregulated in the hypertrophic region, which is invaded by blood vessels during enchondral ossification. In vitro and in vivo assays with the purified protein have shown chondrocyte-modulating a...

journal_title：Molecular and cellular biology

authors： Brandau O,Aszódi A,Hunziker EB,Neame PJ,Vestweber D,Fässler R

更新日期：2002-09-01 00:00:00

abstract：:The pancreatic-islet-enriched transcription factors MafA and MafB have unique expression patterns in β cells in rodents. MafA is specifically expressed in β cells and is a key regulatory factor for maintaining adult β-cell function, whereas MafB plays an essential role in β-cell development during embryogenesis, and i...

journal_title：Molecular and cellular biology

authors： Xiafukaiti G,Maimaiti S,Ogata K,Kuno A,Kudo T,Shawki HH,Oishi H,Takahashi S

更新日期：2019-08-12 00:00:00

abstract：:The regulation of a thyroid hormone-responsive gene in rats, designated spot 14, was explored. The expression of this gene in liver is rapidly (less than 10 min) and markedly (greater than 10-fold) altered by the administration of 3,5,3'-triiodo-L-thyronine (T3) to hypothyroid rats (P. Narayan, C. W. Liaw, and H. C. T...

journal_title：Molecular and cellular biology

authors： Narayan P,Towle HC

更新日期：1985-10-01 00:00:00

abstract：:Synonymous single nucleotide polymorphisms (SNPs) within a transcript's coding region produce no change in the amino acid sequence of the protein product and are therefore intuitively assumed to have a neutral effect on protein function. We report that two common variants of high-temperature requirement A1 (HTRA1) tha...

journal_title：Molecular and cellular biology

authors： Jacobo SM,Deangelis MM,Kim IK,Kazlauskas A

更新日期：2013-05-01 00:00:00

abstract：:R-loops have been described in vivo at the immunoglobulin class switch sequences and at prokaryotic and mitochondrial origins of replication. However, the biochemical mechanism and determinants of R-loop formation are unclear. We find that R-loop formation is nearly eliminated when RNase T(1) is added during transcrip...

journal_title：Molecular and cellular biology

authors： Roy D,Yu K,Lieber MR

更新日期：2008-01-01 00:00:00

abstract：:The Surf-3 gene of the unusually tight mouse Surfeit locus gene cluster has been identified as the highly conserved ribosomal protein gene L7a (rpL7a). The topography and juxtaposition of the Surfeit locus genes are conserved for the 600 million years of divergent evolution between mammals and birds. This suggests cis...

journal_title：Molecular and cellular biology

authors： Armes N,Fried M

更新日期：1995-05-01 00:00:00

abstract：:Cks proteins associate with cyclin-dependent kinases and have therefore been assumed to play a direct role in cell cycle regulation. Mammals have two paralogs, Cks1 and Cks2, and individually deleting the gene encoding either in the mouse has previously been shown not to impact viability. In this study we show that si...

journal_title：Molecular and cellular biology

authors： Martinsson-Ahlzén HS,Liberal V,Grünenfelder B,Chaves SR,Spruck CH,Reed SI

更新日期：2008-09-01 00:00:00

abstract：:The structure of replication origins in metazoans is only nominally similar to that in model organisms, such as Saccharomyces cerevisiae. By contrast to the compact origins of budding yeast, in metazoans multiple elements act as replication start sites or control replication efficiency. We first reported that replicat...

journal_title：Molecular and cellular biology

authors： Liu G,Malott M,Leffak M

更新日期：2003-03-01 00:00:00

abstract：:It is thought that degradation of nuclear proteins by the ubiquitylation system requires nuclear-cytoplasmic trafficking of E3 ubiquitin ligases. The von Hippel-Lindau (VHL) tumor suppressor protein is the substrate recognition component of a Cullin-2-containing E3 ubiquitin ligase that recruits hypoxia-inducible fact...

journal_title：Molecular and cellular biology

authors： Khacho M,Mekhail K,Pilon-Larose K,Payette J,Lee S

更新日期：2008-01-01 00:00:00

abstract：:The mechanism by which the maize autonomous Ac transposable element gives rise to nonautonomous Ds elements is largely unknown. Sequence analysis of native maize Ds elements indicates a complex chimeric structure formed through deletions of Ac sequences with or without insertions of Ac-unrelated sequence blocks. These...

journal_title：Molecular and cellular biology

authors： Rubin E,Levy AA

更新日期：1997-11-01 00:00:00

abstract：:B52, an essential SR protein of Drosophila melanogaster, stimulates pre-mRNA splicing in splicing-deficient mammalian S100 extracts. Surprisingly, mutant larvae depleted of B52 were found to be capable of splicing at least several pre-mRNAs tested (H. Z. Ring and J. T. Lis, Mol. Cell. Biol. 14:7499-7506, 1994). In a h...

journal_title：Molecular and cellular biology

authors： Hoffman BE,Lis JT

更新日期：2000-01-01 00:00:00

abstract：:Revertants were selected from aryl hydrocarbon hydroxylase (AHH)-deficient recessive mutants belonging to three complementation groups and from a dominant mutant of the Hepa-1 cell line. The recessive mutants had low spontaneous reversion frequencies (less than 4 X 10(-7] that were increased by mutagenesis. The majori...

journal_title：Molecular and cellular biology

authors： Van Gurp JR,Hankinson O

更新日期：1984-08-01 00:00:00

abstract：:In addition to the highly conserved AATAAA sequence, there is a requirement for specific sequences downstream of polyadenylic acid [poly(A)] cleavage sites to generate correct mRNA 3' termini. Previous experiments demonstrated that 35 nucleotides downstream of the E2A poly(A) site were sufficient but 20 nucleotides we...

journal_title：Molecular and cellular biology

authors： Hart RP,McDevitt MA,Ali H,Nevins JR

更新日期：1985-11-01 00:00:00

abstract：:To investigate the mechanism of action of volatile anesthetics, we are studying mutants of the yeast Saccharomyces cerevisiae that have altered sensitivity to isoflurane, a widely used clinical anesthetic. Several lines of evidence from these studies implicate a role for ubiquitin metabolism in cellular response to vo...

journal_title：Molecular and cellular biology

authors： Wolfe D,Reiner T,Keeley JL,Pizzini M,Keil RL

更新日期：1999-12-01 00:00:00