Regulated expression of a mammalian nonsense suppressor tRNA gene in vivo and in vitro using the lac operator/repressor system.

abstract：:Cwc25 has previously been identified to associate with pre-mRNA splicing factor Cef1/Ntc85, a component of the Prp19-associated complex (nineteen complex, or NTC) involved in spliceosome activation. We show here that Cwc25 is neither tightly associated with NTC nor required for spliceosome activation but is required f...

journal_title：Molecular and cellular biology

authors： Chiu YF,Liu YC,Chiang TW,Yeh TC,Tseng CK,Wu NY,Cheng SC

更新日期：2009-11-01 00:00:00

abstract：:In Drosophila melanogaster, achaete (ac) and m8 are model basic helix-loop-helix activator (bHLH A) and repressor genes, respectively, that have the opposite cell expression pattern in proneural clusters during Notch signaling. Previous studies have shown that activation of m8 transcription in specific cells within pr...

journal_title：Molecular and cellular biology

authors： Cave JW,Xia L,Caudy M

更新日期：2011-01-01 00:00:00

abstract：:Nuclear migration and positioning within cells are critical for many developmental processes and are governed by the cytoskeletal network. Although mechanisms of nuclear-cytoskeletal attachment are unclear, growing evidence links a novel family of nuclear envelope (NE) proteins that share a conserved C-terminal SUN (S...

journal_title：Molecular and cellular biology

authors： Haque F,Lloyd DJ,Smallwood DT,Dent CL,Shanahan CM,Fry AM,Trembath RC,Shackleton S

更新日期：2006-05-01 00:00:00

abstract：:The baker's yeast Saccharomyces cerevisiae expresses three homologues of the Nramp family of metal transporters: Smf1p, Smf2p, and Smf3p, encoded by SMF1, SMF2, and SMF3, respectively. Here we report a comparative analysis of the yeast Smf proteins at the levels of localization, regulation, and function of the corresp...

journal_title：Molecular and cellular biology

authors： Portnoy ME,Liu XF,Culotta VC

更新日期：2000-11-01 00:00:00

abstract：:Posttranscriptional and posttranslational modification of macromolecules is known to fine-tune their functions. Trm112 is unique, acting as an activator of both tRNA and protein methyltransferases. Here we report that in Saccharomyces cerevisiae, Trm112 is required for efficient ribosome synthesis and progression thro...

journal_title：Molecular and cellular biology

authors： Figaro S,Wacheul L,Schillewaert S,Graille M,Huvelle E,Mongeard R,Zorbas C,Lafontaine DL,Heurgué-Hamard V

更新日期：2012-06-01 00:00:00

abstract：:Mammalian poly(ADP-ribose) polymerase (PARP) is a nuclear chromatin-associated protein with a molecular mass of 114 kDa that catalyzes the transfer of ADP-ribose units from NAD+ to nuclear proteins that are located within chromatin. We report here the identification of a novel property of PARP as a modulator of nuclea...

journal_title：Molecular and cellular biology

authors： Miyamoto T,Kakizawa T,Hashizume K

更新日期：1999-04-01 00:00:00

abstract：:Proteins destined for the nucleus contain nuclear localization sequences, short stretches of amino acids responsible for targeting them to the nucleus. We show that the first 29 amino acids of GAL4, a yeast DNA-binding protein, function efficiently as a nuclear localization sequence when fused to normally cytoplasmic ...

journal_title：Molecular and cellular biology

authors： Nelson M,Silver P

更新日期：1989-02-01 00:00:00

abstract：:We examined the mechanisms that control the downregulation of the c-myc mRNA during differentiation of HL60 cells. On treatment with dimethyl sulfoxide, HL60 cells downmodulated their steady-state c-myc message levels, ceased to proliferate, and underwent terminal differentiation. In nuclear run-on assays in which dis...

journal_title：Molecular and cellular biology

authors： Siebenlist U,Bressler P,Kelly K

更新日期：1988-02-01 00:00:00

abstract：:A gene encoding a novel CACCC box-binding protein that binds to the promoter region of the human T-cell receptor (TCR) V beta 8.1 gene and the mouse TCR alpha gene silencer has been cloned. This gene, termed ht beta, contains four zinc fingers of the class Cys2-X12-His2 that may be responsible for DNA binding and a hi...

journal_title：Molecular and cellular biology

authors： Wang Y,Kobori JA,Hood L

更新日期：1993-09-01 00:00:00

abstract：:In vitro mutagenesis was used to create putative opal suppressor alleles of a tRNA(Trp) gene of Saccharomyces cerevisiae. The construct with the requisite anticodon change did not result in an active suppressor, whereas when a second change was introduced into the portion of the gene encoding the intron, an active and...

journal_title：Molecular and cellular biology

authors： Atkin AL,Roy KL,Bell JB

更新日期：1990-08-01 00:00:00

abstract：:We have isolated a gene, pmk1+, a third mitogen-activated protein kinase (MAPK) gene homolog from the fission yeast Schizosaccharomyces pombe. The predicted amino acid sequence shows the most homology (63 to 65% identity) to those of budding yeast Saccharomyces Mpk1 and Candida Mkc1. The Pmk1 protein contains phosphor...

journal_title：Molecular and cellular biology

authors： Toda T,Dhut S,Superti-Furga G,Gotoh Y,Nishida E,Sugiura R,Kuno T

更新日期：1996-12-01 00:00:00

abstract：:Current amino acid and monosaccharide transport models are based on an assumption which equates the intracellular chemical activity of a solute with its concentration. This assumption was tested for alpha-aminoisobutyric acid and 3-O-methylglucose in a giant cell, the amphibian oocyte, by using recently developed cryo...

journal_title：Molecular and cellular biology

authors： Horowitz SB,Pearson TW

更新日期：1981-09-01 00:00:00

abstract：:The gene coding for the most abundant antifreeze protein (AFP) in the winter flounder was placed downstream of the Drosophila melanogaster hsp70 promoter and introduced into the D. melanogaster germ line by P-element-mediated transformation. In each of six transgenic strains tested, heat shock treatment induced the ex...

journal_title：Molecular and cellular biology

authors： Rancourt DE,Walker VK,Davies PL

更新日期：1987-06-01 00:00:00

abstract：:The expression of chromosomally integrated transgenes usually varies greatly among independent transfectants. This variability in transgene expression has led to the definition of locus control regions (LCRs) as elements which render expression consistent. Analyses of expression in single cells revealed that the expre...

journal_title：Molecular and cellular biology

authors： Ronai D,Berru M,Shulman MJ

更新日期：1999-10-01 00:00:00

abstract：:Purified simian virus 40 (SV40) DNA is reconstituted into chromatin and transcribed by endogenous RNA polymerase II when microinjected into nuclei of Xenopus laevis oocytes. We have correlated the kinetics of chromatin reconstitution with that of accumulation of virus-specific RNA in this system. A delay of approximat...

journal_title：Molecular and cellular biology

authors： Miller TJ,Mertz JE

更新日期：1982-12-01 00:00:00

abstract：:NF-κB activation is a critical component in the transcriptional response to hypoxia. However, the underlying mechanisms that control its activity under these conditions are unknown. Here we report that under hypoxic conditions, IκB kinase (IKK) activity is induced through a calcium/calmodulin-dependent kinase 2 (CaMK2...

journal_title：Molecular and cellular biology

authors： Culver C,Sundqvist A,Mudie S,Melvin A,Xirodimas D,Rocha S

更新日期：2010-10-01 00:00:00

abstract：:In in vitro splicing reactions, influenza virus NS1 mRNA was not detectably spliced, but nonetheless very efficiently formed ATP-dependent 55S complexes containing the U1, U2, U4, U5, and U6 small nuclear ribonucleoproteins (snRNPs) (C. H. Agris, M. E. Nemeroff, and R. M. Krug, Mol. Cell. Biol. 9:259-267, 1989). We de...

journal_title：Molecular and cellular biology

authors： Nemeroff ME,Utans U,Krämer A,Krug RM

更新日期：1992-03-01 00:00:00

abstract：:The folate receptor type alpha (FR-alpha) is a promising tumor marker and target. Here, we investigate the mechanistic basis for the tumor specificity and vast overexpression of FR-alpha. Among representative FR-alpha-positive (HeLa and JAR) and FR-alpha-negative (MG63, Caki1, and HT3) cell lines, the transcription ra...

journal_title：Molecular and cellular biology

authors： Zheng X,Kelley K,Elnakat H,Yan W,Dorn T,Ratnam M

更新日期：2003-03-01 00:00:00

abstract：:In a previous paper, we have shown that in the absence of stress, mouse embryonal carcinoma cells, like mouse early embryo multipotent cells, synthesize high levels of 89- and 70-kilodalton heat shock proteins (HSP)(O. Bensaude and M. Morange, EMBO J. 2:173-177, 1983). We report here the pattern of proteins synthesize...

journal_title：Molecular and cellular biology

authors： Morange M,Diu A,Bensaude O,Babinet C

更新日期：1984-04-01 00:00:00

abstract：:We have identified a gene that encodes the polypeptide cytochrome b in the avian malarial parasite Plasmodium gallinaceum. The gene containing the open reading frame was found to be located on a 6.2-kilobase multimeric extrachromosomal element. The amino acid translation from this gene demonstrated significant similar...

journal_title：Molecular and cellular biology

authors： Aldritt SM,Joseph JT,Wirth DF

更新日期：1989-09-01 00:00:00

abstract：:Sucrase-isomaltase (SI) is an enterocyte-specific gene which exhibits a complex pattern of expression during intestinal development and in the adult intestinal mucosa. In the studies described in this report, we demonstrate that enterocyte-specific transcription of the SI gene is regulated by an evolutionarily conserv...

journal_title：Molecular and cellular biology

authors： Traber PG,Wu GD,Wang W

更新日期：1992-08-01 00:00:00

abstract：:The simian virus 40 large T antigen (T antigen) inactivates tumor suppressor proteins and therefore has been used in numerous studies to probe the mechanisms that control cellular growth and to generate immortalized cell lines. Binding of T antigen to the Rb family of growth-regulatory proteins is necessary but not su...

journal_title：Molecular and cellular biology

authors： Sullivan CS,Cantalupo P,Pipas JM

更新日期：2000-09-01 00:00:00

abstract：:The oncogene product of the avian sarcoma virus CT10, P47gag-crk, contains the SH2, SH2', and SH3 domains and binds proteins in a phosphotyrosine (ptyr)-dependent manner. In this study, we have determined the region of P47gag-crk essential for binding to ptyr-containing proteins. Mutant P47gag-crk proteins expressed i...

journal_title：Molecular and cellular biology

authors： Matsuda M,Mayer BJ,Hanafusa H

更新日期：1991-03-01 00:00:00

abstract：:The pocket protein family of tumor suppressors, and Rb specifically, have been implicated as controlling terminal differentiation in many tissues, including the heart. To establish the biological functions of Rb in the heart and overcome the early lethality caused by germ line deletion of Rb, we used a Cre/loxP system...

journal_title：Molecular and cellular biology

authors： MacLellan WR,Garcia A,Oh H,Frenkel P,Jordan MC,Roos KP,Schneider MD

更新日期：2005-03-01 00:00:00

abstract：:The calcineurin-nuclear factor of activated T cells (NFAT) signaling pathway has been shown to be of critical importance in regulating the growth response of cardiac myocytes. We have previously demonstrated that calcineurin A(beta) (CnA(beta)) mRNA and protein are increased in response to growth stimulation, although...

journal_title：Molecular and cellular biology

authors： Oka T,Dai YS,Molkentin JD

更新日期：2005-08-01 00:00:00

abstract：:Dominant mutations in the early growth response 2 (Egr2/Krox20) transactivator, a critical regulator of peripheral myelin development, have been associated with peripheral myelinopathies. These dominant mutants interfere with the expression of genes required for myelination by Schwann cells, including that for the mos...

journal_title：Molecular and cellular biology

authors： LeBlanc SE,Ward RM,Svaren J

更新日期：2007-05-01 00:00:00

abstract：:In order to determine whether partial methylation of the herpes simplex virus (HSV) tk gene prevents tk gene expression, the HSV tk gene was cloned as single-stranded DNA. By in vitro second-strand DNA synthesis, specific HSV tk gene segments were methylated, and the hemimethylated DNA molecules were microinjected int...

journal_title：Molecular and cellular biology

authors： Graessmann A,Sandberg G,Guhl E,Graessmann M

更新日期：1994-03-01 00:00:00

abstract：:To define the role of Irx4, a member of the Iroquois family of homeobox transcription factors in mammalian heart development and function, we disrupted the murine Irx4 gene. Cardiac morphology in Irx4-deficient mice (designated Irx4(Delta ex2/Delta ex2)) was normal during embryogenesis and in early postnatal life. Adu...

journal_title：Molecular and cellular biology

authors： Bruneau BG,Bao ZZ,Fatkin D,Xavier-Neto J,Georgakopoulos D,Maguire CT,Berul CI,Kass DA,Kuroski-de Bold ML,de Bold AJ,Conner DA,Rosenthal N,Cepko CL,Seidman CE,Seidman JG

更新日期：2001-03-01 00:00:00

abstract：:The opposing effects on proliferation mediated by G-protein-coupled receptor isoforms differing in their COOH termini could be correlated with the abilities of the receptors to differentially activate p38, implicated in apoptotic events, or phosphatidylinositol 3-kinase (PI 3-K), which provides a source of survival si...

journal_title：Molecular and cellular biology

authors： Sellers LA,Alderton F,Carruthers AM,Schindler M,Humphrey PP

更新日期：2000-08-01 00:00:00

abstract：:V(D)J recombination requires binding and synapsis of a complementary (12/23) pair of recombination signal sequences (RSSs) by the RAG1 and RAG2 proteins, aided by a high-mobility group protein, HMG1 or HMG2. Double-strand DNA cleavage within this synaptic, or paired, complex is thought to involve DNA distortion or mel...

journal_title：Molecular and cellular biology

authors： Ciubotaru M,Schatz DG

更新日期：2004-10-01 00:00:00