Template structural requirements for transcription in vivo by RNA polymerase II.

abstract：:Fos and Jun transcription factors are induced during the normal course of the proliferative response of quiescent cells to serum or to growth factors. We have shown that delta FosB, an alternatively spliced form of FosB, is formed as rapidly as FosB in serum-stimulated Rat-1A cells. Although delta FosB lacks the C-ter...

journal_title：Molecular and cellular biology

authors： Nakabeppu Y,Oda S,Sekiguchi M

更新日期：1993-07-01 00:00:00

abstract：:The interferon (IFN)-induced double-stranded RNA-activated protein kinase PKR mediates inhibition of protein synthesis through phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (eIF2alpha) and is also involved in the induction of the IFN gene through the activation of the transcription factor NF-k...

journal_title：Molecular and cellular biology

authors： Bonnet MC,Weil R,Dam E,Hovanessian AG,Meurs EF

更新日期：2000-07-01 00:00:00

abstract：:To examine the role of clathrin-dependent insulin receptor internalization in insulin-stimulated signal transduction events, we expressed a dominant-interfering mutant of dynamin (K44A/dynamin) by using a recombinant adenovirus in the H4IIE hepatoma and 3T3L1 adipocyte cell lines. Expression of K44A/dynamin inhibited ...

journal_title：Molecular and cellular biology

authors： Ceresa BP,Kao AW,Santeler SR,Pessin JE

更新日期：1998-07-01 00:00:00

abstract：:Fibroblasts transformed by Fos oncogenes display increased expression of a number of genes implicated in tumor cell invasion and metastasis. In contrast to normal 208F rat fibroblasts, Fos-transformed 208F fibroblasts are growth factor independent for invasion. We demonstrate that invasion of v-Fos- or epidermal growt...

journal_title：Molecular and cellular biology

authors： Lamb RF,Hennigan RF,Turnbull K,Katsanakis KD,MacKenzie ED,Birnie GD,Ozanne BW

更新日期：1997-02-01 00:00:00

abstract：:Autoimmune diseases are characterized by spontaneously occurring autoantibodies which have proven to be useful reagents for the characterization of specific nuclear proteins. Using a monoclonal autoantibody (72B9) derived from a murine lupus strain, we have cloned a cDNA from the human T-cell line MOLT-4, which encode...

journal_title：Molecular and cellular biology

authors： Pollard KM,Chan EK,Grant BJ,Sullivan KF,Tan EM,Glass CA

更新日期：1990-05-01 00:00:00

abstract：:A previously uncharacterized Saccharomyces cerevisiae gene, FAL1, was found by sequence comparison as a homolog of the eukaryotic translation initiation factor 4A (eIF4A). Fal1p has 55% identity and 73% similarity on the amino acid level to yeast eIF4A, the prototype of ATP-dependent RNA helicases of the DEAD-box prot...

journal_title：Molecular and cellular biology

authors： Kressler D,de la Cruz J,Rojo M,Linder P

更新日期：1997-12-01 00:00:00

abstract：:The retinoblastoma tumor suppressor protein (Rb) plays a vital role in regulating mammalian cell cycle progression and inactivation of Rb is necessary for entry into S phase. Rb is inactivated by phosphorylation upon growth factor stimulation of quiescent cells, facilitating the transition from G(1) phase to S phase. ...

journal_title：Molecular and cellular biology

authors： Dasgupta P,Sun J,Wang S,Fusaro G,Betts V,Padmanabhan J,Sebti SM,Chellappan SP

更新日期：2004-11-01 00:00:00

abstract：:In a previous paper, we have shown that in the absence of stress, mouse embryonal carcinoma cells, like mouse early embryo multipotent cells, synthesize high levels of 89- and 70-kilodalton heat shock proteins (HSP)(O. Bensaude and M. Morange, EMBO J. 2:173-177, 1983). We report here the pattern of proteins synthesize...

journal_title：Molecular and cellular biology

authors： Morange M,Diu A,Bensaude O,Babinet C

更新日期：1984-04-01 00:00:00

abstract：:Testis-brain RNA-binding protein (TB-RBP), the mouse orthologue of the human protein Translin, is a widely expressed and highly conserved protein with proposed functions in chromosomal translocations, mitotic cell division, and mRNA transport and storage. To better define the biological roles of TB-RBP, we generated m...

journal_title：Molecular and cellular biology

authors： Chennathukuzhi V,Stein JM,Abel T,Donlon S,Yang S,Miller JP,Allman DM,Simmons RA,Hecht NB

更新日期：2003-09-01 00:00:00

abstract：:Hox gene functions are intimately linked to correct developmental expression of the genes. The identification of cis-acting regulatory sequences and their associated trans-acting factors constitutes a key step in deciphering the mechanisms underlying the correct positioning of the functional domain of Hox genes along ...

journal_title：Molecular and cellular biology

authors： Tabariès S,Lapointe J,Besch T,Carter M,Woollard J,Tuggle CK,Jeannotte L

更新日期：2005-02-01 00:00:00

abstract：:Autophagy is considered an adaptive mechanism against hyperosmotic stress. Although the process has been reported to be triggered by the inhibition of mTORC1, the precise downstream mechanisms remain elusive. Here, we demonstrate that hyperosmotic-stress-induced autophagy is different from conventional macroautophagy ...

journal_title：Molecular and cellular biology

authors： Tamura N,Kageyama S,Komatsu M,Waguri S

更新日期：2019-07-29 00:00:00

abstract：:The baker's yeast Saccharomyces cerevisiae possesses a single gene encoding heat shock transcription factor (HSF), which is required for the activation of genes that participate in stress protection as well as normal growth and viability. Yeast HSF (yHSF) contains two distinct transcriptional activation regions locate...

journal_title：Molecular and cellular biology

authors： Santoro N,Johansson N,Thiele DJ

更新日期：1998-11-01 00:00:00

abstract：:Drosophila hsp70 genes have an RNA polymerase II molecule paused at their 5' ends in uninduced cells. In this study we have shown that this pausing also occurs on other heat shock and constitutively expressed genes. We propose that a rate-limiting step in early elongation occurs in many Drosophila genes and may be a t...

journal_title：Molecular and cellular biology

authors： Rougvie AE,Lis JT

更新日期：1990-11-01 00:00:00

abstract：:The glucose-activated transcription factor carbohydrate response element binding protein (ChREBP) induces the expression of hepatic glycolytic and lipogenic genes. The farnesoid X receptor (FXR) is a nuclear bile acid receptor controlling bile acid, lipid, and glucose homeostasis. FXR negatively regulates hepatic glyc...

journal_title：Molecular and cellular biology

authors： Caron S,Huaman Samanez C,Dehondt H,Ploton M,Briand O,Lien F,Dorchies E,Dumont J,Postic C,Cariou B,Lefebvre P,Staels B

更新日期：2013-06-01 00:00:00

abstract：:The CYC1-239-O mutation in the yeast Saccharomyces cerevisiae produces a -His-Leu- replacement of the normal -Ala-Gly- sequence at amino acid positions 5 and 6, which lie within a dispensable region of iso-1-cytochrome c; this mutation can accommodate the formation of a hairpin structure at the corresponding site in t...

journal_title：Molecular and cellular biology

authors： Baim SB,Pietras DF,Eustice DC,Sherman F

更新日期：1985-08-01 00:00:00

abstract：:The mechanism(s) and site(s) of action of volatile inhaled anesthetics are unknown in spite of the clinical use of these agents for more than 150 years. In the present study, the model eukaryote Saccharomyces cerevisiae was used to investigate the action of anesthetic agents because of its powerful molecular genetics....

journal_title：Molecular and cellular biology

authors： Keil RL,Wolfe D,Reiner T,Peterson CJ,Riley JL

更新日期：1996-07-01 00:00:00

abstract：:We investigated the role of RNA polymerase II (pol II) carboxy-terminal domain (CTD) phosphorylation in pre-mRNA processing coupled and uncoupled from transcription in Xenopus oocytes. Inhibition of CTD phosphorylation by the kinase inhibitors 5,6-dichloro-1beta-D-ribofuranosyl-benzimidazole and H8 blocked transcripti...

journal_title：Molecular and cellular biology

authors： Bird G,Zorio DA,Bentley DL

更新日期：2004-10-01 00:00:00

abstract：:In Chlamydomonas reinhardi the chloroplast DNA (ch;DNA) of mating type plus cells undergoes cyclical methylation and demethylation during the life cycle. Methylation occurs during gametogenesis, and fully differentiated gametes can be dedifferentiated back to vegetative cells which contain nonmethylated chlDNA by the ...

journal_title：Molecular and cellular biology

authors： Sano H,Grabowy C,Sager R

更新日期：1984-10-01 00:00:00

abstract：:Selective and accurate transcription of purified genes by RNA polymerase II requires multiple factors. The factor designated TFIID was purified extensively from HeLa cell nuclear extracts by using a simple and novel complementation assay. Thus, TFIID was preferentially inactivated by mild heat treatment of a nuclear e...

journal_title：Molecular and cellular biology

authors： Nakajima N,Horikoshi M,Roeder RG

更新日期：1988-10-01 00:00:00

abstract：:Although globin mRNAs are considered prototypes of highly stable messages, the mechanisms responsible for their longevity remain largely undefined. As an initial step in identifying potential cis-acting elements or structures which contribute to their stability, we analyzed the defect in expression of a naturally occu...

journal_title：Molecular and cellular biology

authors： Weiss IM,Liebhaber SA

更新日期：1994-12-01 00:00:00

abstract：:Cells respond to environmental stimuli by fine-tuned regulation of gene expression. Here we investigated the dose-dependent modulation of gene expression at high temporal resolution in response to nutrient and stress signals in yeast. The GAL1 activity in cell populations is modulated in a well-defined range of galact...

journal_title：Molecular and cellular biology

authors： Rienzo A,Poveda-Huertes D,Aydin S,Buchler NE,Pascual-Ahuir A,Proft M

更新日期：2015-11-01 00:00:00

abstract：:The upstream activating sequence of the adjacent and divergently transcribed GAL1 and GAL10 genes of Saccharomyces cerevisiae (UASG) contains at least three distinct classes of overlapping transcriptional control sites. The transcription activator GAL4 binds to four related sites in UASG and induces expression of GAL1...

journal_title：Molecular and cellular biology

authors： Finley RL Jr,West RW Jr

更新日期：1989-10-01 00:00:00

abstract：:Our recent studies implicated key and distinct roles for the highly related RalA and RalB small GTPases (82% sequence identity) in pancreatic ductal adenocarcinoma (PDAC) tumorigenesis and invasive and metastatic growth, respectively. How RalB may promote PDAC invasion and metastasis has not been determined. In light ...

journal_title：Molecular and cellular biology

authors： Neel NF,Rossman KL,Martin TD,Hayes TK,Yeh JJ,Der CJ

更新日期：2012-04-01 00:00:00

abstract：:The mouse urokinase-type plasminogen activator (uPA) gene was used as a model macrophage colony-stimulating factor 1 (CSF-1)-inducible gene to investigate CSF-1 signalling pathways. Nuclear run-on analysis showed that induction of uPA mRNA by CSF-1 and phorbol myristate acetate (PMA) was at the transcriptional level i...

journal_title：Molecular and cellular biology

authors： Stacey KJ,Fowles LF,Colman MS,Ostrowski MC,Hume DA

更新日期：1995-06-01 00:00:00

abstract：:The ErbB family includes four homologous transmembrane tyrosine kinases. Whereas ErbB-1 binds to the epidermal growth factor (EGF), both ErbB-3 and ErbB-4 bind to the Neu differentiation factors (NDFs, or neuregulins), and ErbB-2, the most oncogenic family member, is an orphan receptor whose function is still unknown....

journal_title：Molecular and cellular biology

authors： Tzahar E,Waterman H,Chen X,Levkowitz G,Karunagaran D,Lavi S,Ratzkin BJ,Yarden Y

更新日期：1996-10-01 00:00:00

abstract：:Correct reprogramming of epigenetic marks in the donor nuclei is crucial for successful cloning by nuclear transfer. Specific epigenetic modifications, such as repressive histone lysine methylation marks, are known to be very stable and difficult to reprogram. The discovery of histone lysine demethylases has opened up...

journal_title：Molecular and cellular biology

authors： Antony J,Oback F,Chamley LW,Oback B,Laible G

更新日期：2013-03-01 00:00:00

abstract：:Hox11 is a homeobox gene essential for spleen formation in mice, since atrophy of the anlage of a developing spleen occurs in early embryonic development in Hox11 null mice. HOX11 is also expressed in a subset of T-cell acute leukemias after specific chromosomal translocations. Since the protein has a homeodomain and ...

journal_title：Molecular and cellular biology

authors： Greene WK,Bahn S,Masson N,Rabbitts TH

更新日期：1998-12-01 00:00:00

abstract：:The PH domain-containing proteins Slm1 and Slm2 were previously identified as effectors of the phosphatidylinositol-4,5-bisphosphate (PI4,5P(2)) and TORC2 signaling pathways. Here, we demonstrate that Slm1 and Slm2 are also targets of sphingolipid signaling during the heat shock response. We show that upon depletion o...

journal_title：Molecular and cellular biology

authors： Daquinag A,Fadri M,Jung SY,Qin J,Kunz J

更新日期：2007-01-01 00:00:00

abstract：:Individual steps in the processing of pre-mRNA, including 5'-end cap formation, splicing, and 3'-end processing (cleavage and polyadenylation) are highly integrated and can influence one another. In addition, prior splicing can influence downstream steps in gene expression, including export of mRNA from the nucleus. H...

journal_title：Molecular and cellular biology

authors： McCracken S,Lambermon M,Blencowe BJ

更新日期：2002-01-01 00:00:00

abstract：:To identify novel genes that play critical roles in mediating bone morphogenetic protein (BMP) signal pathways, we performed a yeast two-hybrid screen using Smad1 as bait. A novel mouse Krüppel-type zinc finger protein, mZnf8, was isolated. Interactions between mZnf8 and Smad proteins were further analyzed with variou...

journal_title：Molecular and cellular biology

authors： Jiao K,Zhou Y,Hogan BL

更新日期：2002-11-01 00:00:00