Construction of an opal suppressor by oligonucleotide-directed mutagenesis of a Saccharomyces cerevisiae tRNA(Trp) gene.

abstract：:The Cyp cell line consists of mouse cells transformed by a thermosensitive polyomavirus (Py) genome and routinely propagated at 39 degrees C. Cyp cells are readily induced to synthesize free Py DNA by being transferred to 33 degrees C. In one subclone (C12/a1/S48, or S48) of this line, such induction resulted in the i...

journal_title：Molecular and cellular biology

authors： Huberdeau D,Sylla BS,Herring-Gillam E,Bourgaux-Ramoisy D,Bourgaux P

更新日期：1985-10-01 00:00:00

abstract：:Transfection of C3H/10T1/2 cells with a c-myc gene resulted in the acquisition of responsiveness to transforming growth factor type beta. Cells transfected with an activated H-ras gene or an H-ras and c-myc gene, however, exhibited a transformed morphology and spontaneous soft-agar growth, a phenotype induced reversib...

journal_title：Molecular and cellular biology

authors： Leof EB,Proper JA,Moses HL

更新日期：1987-07-01 00:00:00

abstract：:Cooperation between STAT3 and c-Jun in driving transcription during transfection of reporter constructs is well established, and both proteins are present on some interleukin-6 (IL-6) STAT3-dependent promoters on chromosomal loci. We report that small interfering RNA knockdown of c-Jun or c-Fos diminishes IL-6 inducti...

journal_title：Molecular and cellular biology

authors： Ginsberg M,Czeko E,Müller P,Ren Z,Chen X,Darnell JE Jr

更新日期：2007-09-01 00:00:00

abstract：:A key step in homologous recombination is the loading of Rad51 onto single-stranded DNA to form a nucleoprotein filament that promotes homologous DNA pairing and strand exchange. Mediator proteins, such as Rad52 and Rad55-Rad57, are thought to aid filament assembly by overcoming an inhibitory effect of the single-stra...

journal_title：Molecular and cellular biology

authors： Osman F,Dixon J,Barr AR,Whitby MC

更新日期：2005-09-01 00:00:00

abstract：:The amplified CAD genes in N-(phosphonacetyl)-L-aspartate (PALA)-resistant Syrian hamster cells are located in an expanded chromosomal region emanating from the site of the wild-type gene at the tip of the short arm of chromosome B-9. The terminus of B-9 in PALA-sensitive cells contains a cluster of rRNA genes (i.e., ...

journal_title：Molecular and cellular biology

authors： Wahl GM,Vitto L,Rubnitz J

更新日期：1983-11-01 00:00:00

abstract：:Nuclear factor kappa B (NF-kappa B) is a ubiquitous transcription factor which binds to decameric DNA sequences (kappa B sites) and regulates transcription of multiple genes. The activity of NF-kappa B is regulated by an inhibitor protein, I kappa B, which sequesters NF-kappa B in the cytoplasm. Release of I kappa B a...

journal_title：Molecular and cellular biology

authors： Miyamoto S,Chiao PJ,Verma IM

更新日期：1994-05-01 00:00:00

abstract：:The two beta-tubulin genes of the unicellular green alga Chlamydomonas reinhardtii are expressed coordinately after deflagellation and produce two transcripts of 2.1 and 2.0 kilobases. Full-length cDNA clones corresponding to the transcript of each gene were isolated. DNA sequences were obtained from the cDNA clones a...

journal_title：Molecular and cellular biology

authors： Youngblom J,Schloss JA,Silflow CD

更新日期：1984-12-01 00:00:00

abstract：:Oxygen radicals regulate many physiological processes, such as signaling, proliferation, and apoptosis, and thus play a pivotal role in pathophysiology and disease development. There are at least two thioredoxin reductase/thioredoxin/peroxiredoxin systems participating in the cellular defense against oxygen radicals. ...

journal_title：Molecular and cellular biology

authors： Conrad M,Jakupoglu C,Moreno SG,Lippl S,Banjac A,Schneider M,Beck H,Hatzopoulos AK,Just U,Sinowatz F,Schmahl W,Chien KR,Wurst W,Bornkamm GW,Brielmeier M

更新日期：2004-11-01 00:00:00

abstract：:Simple sequence repeat telomeric DNA is maintained by a specialized reverse transcriptase, telomerase. The integral RNA subunit of telomerase contains a template region that determines the sequence added to chromosome ends. Aside from providing the template, little is known about the role of the telomerase RNA. In add...

journal_title：Molecular and cellular biology

authors： Mitchell JR,Cheng J,Collins K

更新日期：1999-01-01 00:00:00

abstract：:We have analyzed the 5'-flanking region of one of the genes coding for the human acute-phase protein, serum amyloid A (SAA). We found that SAA mRNA could be increased fivefold in transfected cells by treatment with phorbol 12-myristate 13-acetate (PMA). To analyze this observation further, we placed a 265-base-pair 5'...

journal_title：Molecular and cellular biology

authors： Edbrooke MR,Burt DW,Cheshire JK,Woo P

更新日期：1989-05-01 00:00:00

abstract：:The yeast alpha-factor pheromone receptor is a member of the G-protein-coupled receptor family. Limited trypsin digestion of yeast membranes was used to investigate ligand-induced conformational changes in this receptor. The agonist, alpha-factor, accelerated cleavage in the third intracellular loop, whereas the antag...

journal_title：Molecular and cellular biology

authors： Büküşoğlu G,Jenness DD

更新日期：1996-09-01 00:00:00

abstract：:XPC is responsible for DNA damage sensing in nucleotide excision repair (NER). Mutations in XPC lead to a defect in NER and to xeroderma pigmentosum (XP-C). Here, we analyzed the biochemical properties behind mutations found within three patients: one amino acid substitution (P334H, XP1MI, and GM02096), one amino acid...

journal_title：Molecular and cellular biology

authors： Bernardes de Jesus BM,Bjørås M,Coin F,Egly JM

更新日期：2008-12-01 00:00:00

abstract：:Nuclear migration and positioning within cells are critical for many developmental processes and are governed by the cytoskeletal network. Although mechanisms of nuclear-cytoskeletal attachment are unclear, growing evidence links a novel family of nuclear envelope (NE) proteins that share a conserved C-terminal SUN (S...

journal_title：Molecular and cellular biology

authors： Haque F,Lloyd DJ,Smallwood DT,Dent CL,Shanahan CM,Fry AM,Trembath RC,Shackleton S

更新日期：2006-05-01 00:00:00

abstract：:DHCR24/seladin-1, a crucial enzyme in sterol synthesis, is of lower abundance in brain areas affected by Alzheimer's disease. While high levels of DHCR24/seladin-1 exert antiapoptotic function by conferring resistance against oxidative stress, the molecular mechanism for this protective effect is not fully understood....

journal_title：Molecular and cellular biology

authors： Kuehnle K,Crameri A,Kälin RE,Luciani P,Benvenuti S,Peri A,Ratti F,Rodolfo M,Kulic L,Heppner FL,Nitsch RM,Mohajeri MH

更新日期：2008-01-01 00:00:00

abstract：:DNA end-labeling procedures were used to analyze both the frequency and distribution of DNA strand breaks in mammalian cells exposed or not to different types of DNA-damaging agents. The 3' ends were labeled by T4 DNA polymerase-catalyzed nucleotide exchange carried out in the absence or presence of Escherichia coli e...

journal_title：Molecular and cellular biology

authors： Legault J,Tremblay A,Ramotar D,Mirault ME

更新日期：1997-09-01 00:00:00

abstract：:ADP-ribosyltransferase diphtheria-toxin like 1/poly(ADP-ribose) polymerase 1 (ARTD1/PARP1) is a chromatin-associated protein in the nucleus and plays an important role in different cellular processes such as regulation of gene transcription. ARTD1 has been shown to coregulate the inflammatory response by modulating th...

journal_title：Molecular and cellular biology

authors： Minotti R,Andersson A,Hottiger MO

更新日期：2015-09-01 00:00:00

abstract：:Microtubule-associated serine/threonine kinase like (MASTL), also known as Greatwall (Gwl) kinase, has an important role in the regulation of mitosis. By inhibiting protein phosphatase 2A (PP2A), it plays a crucial role in activating one of the most important mitotic kinases, known as cyclin-dependent kinase 1 (CDK1)....

journal_title：Molecular and cellular biology

authors： Reshi I,Nisa MU,Farooq U,Gillani SQ,Bhat SA,Sarwar Z,Nabi N,Fazili KM,Andrabi S

更新日期：2020-04-28 00:00:00

abstract：:CD133, a widely known marker of cancer stem cells, was recently found in extracellular vesicles. However, the mechanisms underlying CD133 translocation to the extracellular space remain largely unknown. Here we report that CD133 is monoubiquitinated. Ubiquitination occurs primarily on complex glycosylated CD133. The l...

journal_title：Molecular and cellular biology

authors： Yang F,Xing Y,Li Y,Chen X,Jiang J,Ai Z,Wei Y

更新日期：2018-07-16 00:00:00

abstract：:Despite detailed knowledge of the components of the spindle assembly checkpoint, a molecular explanation of how cells die after prolonged spindle checkpoint activation, and thus how microtubule inhibitors and other antimitotic drugs ultimately elicit their lethal effects, has yet to emerge. Mitotically arrested cells ...

journal_title：Molecular and cellular biology

authors： Terrano DT,Upreti M,Chambers TC

更新日期：2010-02-01 00:00:00

abstract：:Cells of Candida albicans WO-1 spontaneously switch between a white and opaque CFU, and this phase transition involves a dramatic change in cellular phenotype. By using a differential hybridization screen, an opaque-specific cDNA, Op1a, which represents the transcript of a gene regulated by switching, has been isolate...

journal_title：Molecular and cellular biology

authors： Morrow B,Srikantha T,Soll DR

更新日期：1992-07-01 00:00:00

abstract：:Low-molecular-weight RNA exhibiting transforming potential was identified in chemically induced lymphoma cells by the transformation of mink lung cells after transfection. The RNA was sequenced by the direct chemical method and was shown to be a small nuclear RNA, U5. The transforming potential of the RNA was further ...

journal_title：Molecular and cellular biology

authors： Hamada K,Kumazaki T,Mizuno K,Yokoro K

更新日期：1989-10-01 00:00:00

abstract：:The eukaryotic genome is divided into chromosomal domains of distinct gene activities. Transcriptionally silent chromatin tends to encroach upon active chromatin. Barrier elements that can block the spread of silent chromatin have been documented, but the mechanisms of their function are not resolved. We show that the...

journal_title：Molecular and cellular biology

authors： Bi X,Yu Q,Sandmeier JJ,Zou Y

更新日期：2004-03-01 00:00:00

abstract：:The nucleotide sequence of 5'-noncoding and N-terminal coding regions of two coordinately regulated, repressible acid phosphatase genes from Saccharomyces cerevisiae were determined. These unlinked genes encode different, but structurally related polypeptides of molecular weights 60,000 and 56,000. The DNA sequences o...

journal_title：Molecular and cellular biology

authors： Thill GP,Kramer RA,Turner KJ,Bostian KA

更新日期：1983-04-01 00:00:00

abstract：:Drugs that produce covalent interstrand cross-links (ICLs) in DNA remain central to the treatment of cancer, but the cell cycle checkpoints activated by ICLs have received little attention. We have used the fission yeast, Schizosaccharomyces pombe, to elucidate the checkpoint responses to the ICL-inducing anticancer d...

journal_title：Molecular and cellular biology

authors： Lambert S,Mason SJ,Barber LJ,Hartley JA,Pearce JA,Carr AM,McHugh PJ

更新日期：2003-07-01 00:00:00

abstract：:Expression of the acute-phase response genes, such as that for alpha-1 acid glycoprotein (AGP), involves both positive and negative transcription factors. A positive transcription factor, AGP/EBP, and a negative transcription factor, factor B, have been identified as the two most important factors responsible for the ...

journal_title：Molecular and cellular biology

authors： Yang TH,Tsai WH,Lee YM,Lei HY,Lai MY,Chen DS,Yeh NH,Lee SC

更新日期：1994-09-01 00:00:00

abstract：:We examined the effects of 18 h of incubation of Chinese hamster ovary (CHO K1) cells with cycloheximide, hydroxyurea, and aphidicolin. Treatment of cells with cycloheximide alone at a concentration adequate to inhibit DNA synthesis to less than 10% of control was significantly less cytotoxic and clastogenic than trea...

journal_title：Molecular and cellular biology

authors： Sherwood SW,Schumacher RI,Schimke RT

更新日期：1988-07-01 00:00:00

abstract：:GCN4 is a transcriptional activator of amino acid-biosynthetic genes in the yeast Saccharomyces cerevisiae. GCN2, a translational activator of GCN4 expression, contains a domain homologous to the catalytic subunit of eucaryotic protein kinases. Substitution of a highly conserved lysine residue in the kinase domain abo...

journal_title：Molecular and cellular biology

authors： Wek RC,Ramirez M,Jackson BM,Hinnebusch AG

更新日期：1990-06-01 00:00:00

abstract：:Kss1 and Fus3 are mitogen-activated protein kinases (MAPKs or ERKs), and Ste7 is their activating MAPK/ERK kinase (MEK), in the pheromone response pathway of Saccharomyces cerevisiae. To investigate the potential role of specific interactions between these enzymes during signaling, their ability to associate with each...

journal_title：Molecular and cellular biology

authors： Bardwell L,Cook JG,Chang EC,Cairns BR,Thorner J

更新日期：1996-07-01 00:00:00

abstract：:Transcription factor Nrf2 (NF-E2-related factor 2) is essential for oxidative and electrophilic stress responses. While it has been well characterized that Nrf2 activity is tightly regulated at the protein level through proteasomal degradation via Keap1 (Kelch-like ECH-associated protein 1)-mediated ubiquitination, no...

journal_title：Molecular and cellular biology

authors： Suzuki T,Shibata T,Takaya K,Shiraishi K,Kohno T,Kunitoh H,Tsuta K,Furuta K,Goto K,Hosoda F,Sakamoto H,Motohashi H,Yamamoto M

更新日期：2013-06-01 00:00:00

abstract：:The interaction of enhancers with different promoters was studied by measuring the influence of two enhancers (from simian virus 40 and from Harvey sarcoma virus) on the activity of expression vectors that are identical except for their promoter region. The promoters examined were from the simian virus 40 early region...

journal_title：Molecular and cellular biology

authors： Berg PE,Popovic Z,Anderson WF

更新日期：1984-08-01 00:00:00