Purification and characterization of nucleolin and its identification as a transcription repressor.

abstract：:TRIF is an essential adaptor for Toll-like receptor 3/4 (TLR3/4) signaling to activate transcription factor interferon regulatory factor 3 (IRF-3). We examined the molecular mechanism of TLR3 signaling and found that TLR3 stimulation by double-stranded RNA (dsRNA) induces phosphorylation of TRIF at Ser210 and is requi...

journal_title：Molecular and cellular biology

authors： Abe H,Satoh J,Shirasaka Y,Kogure A,Kato H,Ito S,Fujita T

更新日期：2020-02-12 00:00:00

abstract：:Transcriptional coactivator with PDZ-binding motif (TAZ) physically interacts with a variety of transcription factors and modulates their activities involved in cell proliferation and mesenchymal stem cell differentiation. TAZ is highly expressed in the kidney, and a deficiency of this protein results in multiple rena...

journal_title：Molecular and cellular biology

authors： Jang EJ,Jeong H,Han KH,Kwon HM,Hong JH,Hwang ES

更新日期：2012-12-01 00:00:00

abstract：:Treatment of WEHI 231 immature B-lymphoma cells with an antibody against their surface immunoglobulin (anti-Ig) induces apoptosis and has been studied extensively as a model of B-cell tolerance. Anti-Ig treatment of exponentially growing WEHI 231 cells results in an early transient increase in c-myc expression that is...

journal_title：Molecular and cellular biology

authors： Wu M,Arsura M,Bellas RE,FitzGerald MJ,Lee H,Schauer SL,Sherr DH,Sonenshein GE

更新日期：1996-09-01 00:00:00

abstract：:Inactivation of DNA mismatch repair by mutation or by transcriptional silencing of the MLH1 gene results in genome instability and cancer predisposition. We recently found (P. V. Shcherbakova and T. A. Kunkel, Mol. Cell. Biol. 19:3177-3183, 1999) that an elevated spontaneous mutation rate can also result from increase...

journal_title：Molecular and cellular biology

authors： Shcherbakova PV,Hall MC,Lewis MS,Bennett SE,Martin KJ,Bushel PR,Afshari CA,Kunkel TA

更新日期：2001-02-01 00:00:00

abstract：:We have analyzed the interleukin-4 (IL-4)-triggered mechanisms implicated in cell survival and show here that IL-4 deprivation induces apoptotic cell death but does not modulate Bcl-2 or Bcl-x expression. Since Bcl-x expression is insufficient to ensure cell survival in the absence of IL-4, we speculate that additiona...

journal_title：Molecular and cellular biology

authors： Rebollo A,Dumoutier L,Renauld JC,Zaballos A,Ayllón V,Martínez-A C

更新日期：2000-05-01 00:00:00

abstract：:Latent Epstein-Barr virus (EBV) is maintained as a nucleosome-covered episome that can be transcriptionally activated by overexpression of the viral immediate-early protein, Zta. We show here that reactivation of latent EBV by Zta can be significantly enhanced by coexpression of the cellular coactivators CREB binding ...

journal_title：Molecular and cellular biology

authors： Zerby D,Chen CJ,Poon E,Lee D,Shiekhattar R,Lieberman PM

更新日期：1999-03-01 00:00:00

abstract：:The female-sterile ovarian tumor gene, otu, is located in cytological region 7F1 on the Drosophila melanogaster chromosome map. We have mapped the gene at the molecular level by using four dysgenic alleles and two revertant derivatives of these alleles as well as an ethyl methanesulfonate-induced allele. The insertion...

journal_title：Molecular and cellular biology

authors： Mulligan PK,Mohler JD,Kalfayan LJ

更新日期：1988-04-01 00:00:00

abstract：:An ssu72 mutant of Saccharomyces cerevisiae was identified as an enhancer of a TFIIB defect (sua7-1) that confers both a cold-sensitive growth defect and a downstream shift in transcription start site selection. The ssu72-1 allele did not affect cold sensitivity but, in combination with sua7-1, created a heat-sensitiv...

journal_title：Molecular and cellular biology

authors： Sun ZW,Hampsey M

更新日期：1996-04-01 00:00:00

abstract：:The regulatory subunits of cyclic AMP (cAMP)-dependent protein kinase from a dibutyryl cAMP-resistant S49 mouse lymphoma cell mutant, clone U200/65.1, and its revertants were visualized by two-dimensional polyacrylamide gel electrophoresis. Clone U200/65.1 co-expressed electrophoretically distinguishable mutant and wi...

journal_title：Molecular and cellular biology

authors： van Daalen Wetters T,Coffino P

更新日期：1983-02-01 00:00:00

abstract：:NF-kappaB2 (p100/p52), a member of the NF-kappaB/Rel family of transcription factors, is involved in the regulation of a variety of genes important for immune function. Previously, we have shown that the NF-kappaB2 gene is regulated in a positive and a negative manner. Two kappaB elements within the NF-kappaB2 promote...

journal_title：Molecular and cellular biology

authors： Oswald F,Liptay S,Adler G,Schmid RM

更新日期：1998-04-01 00:00:00

abstract：:We have studied the abilities of different transactivation domains to stimulate the initiation and elongation (postinitiation) steps of RNA polymerase II transcription in vivo. Nuclear run-on and RNase protection analyses revealed three classes of activation domains: Sp1 and CTF stimulated initiation (type I); human i...

journal_title：Molecular and cellular biology

authors： Blau J,Xiao H,McCracken S,O'Hare P,Greenblatt J,Bentley D

更新日期：1996-05-01 00:00:00

abstract：:Although the U1 small nuclear ribonucleoprotein particle (snRNP) was the first mRNA-splicing cofactor to be identified, the manner in which it functions in splicing is not precisely understood. Among the information required to understand how U1 snRNP participates in splicing, it will be necessary to know its structur...

journal_title：Molecular and cellular biology

authors： Patton JR,Patterson RJ,Pederson T

更新日期：1987-11-01 00:00:00

abstract：:LBP-1 is a cellular protein which binds strongly to sequences around the human immunodeficiency virus type 1 (HIV-1) initiation site and weakly over the TATA box. We have previously shown that LBP-1 represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA box. Four similar but distinct cDNAs encodi...

journal_title：Molecular and cellular biology

authors： Yoon JB,Li G,Roeder RG

更新日期：1994-03-01 00:00:00

abstract：:Fms is the receptor for macrophage colony-stimulating factor (M-CSF) and contains intrinsic tyrosine kinase activity. Expression of exogenous Fms in a murine myeloid progenitor cell line, FDC-P1 (FD-Fms), results in M-CSF-dependent growth and macrophage differentiation. Previously, we described a 100-kDa protein that ...

journal_title：Molecular and cellular biology

authors： Liu Y,Jenkins B,Shin JL,Rohrschneider LR

更新日期：2001-05-01 00:00:00

abstract：:The yeast protein Spa2p localizes to growth sites and is important for polarized morphogenesis during budding, mating, and pseudohyphal growth. To better understand the role of Spa2p in polarized growth, we analyzed regions of the protein important for its function and proteins that interact with Spa2p. Spa2p interact...

journal_title：Molecular and cellular biology

authors： Sheu YJ,Santos B,Fortin N,Costigan C,Snyder M

更新日期：1998-07-01 00:00:00

abstract：:The human neuroblastoma cell line CHP100 provides a useful model system in which to study the molecular mechanisms of transcriptional regulation of the low-affinity nerve growth factor receptor (NGFR) gene during neuronal development. Basic fibroblast growth factor (bFGF) induced morphological changes in CHP100 cells,...

journal_title：Molecular and cellular biology

authors： Taiji M,Taiji K,Deyerle KL,Bothwell M

更新日期：1992-05-01 00:00:00

abstract：:The ATM protein has been implicated in pathways controlling cell cycle checkpoints, radiosensitivity, genetic instability, and aging. Expression of ATM fragments containing a leucine zipper motif in a human tumor cell line abrogated the S-phase checkpoint after ionizing irradiation and enhanced radiosensitivity and ch...

journal_title：Molecular and cellular biology

authors： Morgan SE,Lovly C,Pandita TK,Shiloh Y,Kastan MB

更新日期：1997-04-01 00:00:00

abstract：:We investigated the interaction of phospholipase C-gamma (PLC-gamma) with wild-type and mutant forms of the platelet-derived growth factor (PDGF) beta-receptor both in vivo and in vitro. After PDGF treatment of CHO cell lines expressing wild-type or either of two mutant (delta Ki and Y825F) PDGF receptors, PLC-gamma b...

journal_title：Molecular and cellular biology

authors： Morrison DK,Kaplan DR,Rhee SG,Williams LT

更新日期：1990-05-01 00:00:00

abstract：:IkappaB kinases (IKKalpha and IKKbeta) are key components of the IKK complex that mediates activation of the transcription factor NF-kappaB in response to extracellular stimuli such as inflammatory cytokines, viral and bacterial infection, and UV irradiation. Although NF-kappaB-inducing kinase (NIK) interacts with and...

journal_title：Molecular and cellular biology

authors： Nemoto S,DiDonato JA,Lin A

更新日期：1998-12-01 00:00:00

abstract：:DHTKD1, a part of 2-ketoadipic acid dehydrogenase complex, is involved in lysine and tryptophan catabolism. Mutations in DHTKD1 block the metabolic pathway and cause 2-aminoadipic and 2-oxoadipic aciduria (AMOXAD), an autosomal recessive inborn metabolic disorder. In addition, a nonsense mutation in DHTKD1 that we ide...

journal_title：Molecular and cellular biology

authors： Xu WY,Zhu H,Shen Y,Wan YH,Tu XD,Wu WT,Tang L,Zhang HX,Lu SY,Jin XL,Fei J,Wang ZG

更新日期：2018-06-14 00:00:00

abstract：:The punc gene, encoding a member of the neural cell adhesion molecule family expressed in the developing central nervous system, limbs, and inner ear, was identified. To extend studies of the normal expression pattern of punc and to determine its function, a mouse strain bearing a lacZ/neo insertion in a 5' coding exo...

journal_title：Molecular and cellular biology

authors： Yang W,Li C,Mansour SL

更新日期：2001-09-01 00:00:00

abstract：:Different models have been proposed explaining how eukaryotic gene transcription is terminated. Recently, Nsi1, a factor involved in silencing of ribosomal DNA (rDNA), was shown to be required for efficient termination of rDNA transcription by RNA polymerase I (Pol I) in the yeast Saccharomyces cerevisiae. Nsi1 contai...

journal_title：Molecular and cellular biology

authors： Merkl P,Perez-Fernandez J,Pilsl M,Reiter A,Williams L,Gerber J,Böhm M,Deutzmann R,Griesenbeck J,Milkereit P,Tschochner H

更新日期：2014-10-01 00:00:00

abstract：:Nuclear expression and consequent biological action of the eukaryotic NF-kappa B transcription factor complex are tightly regulated through its cytoplasmic retention by an ankyrin-rich inhibitory protein termed I kappa B alpha. I kappa B alpha specifically binds to and masks the nuclear localization signal of the RelA...

journal_title：Molecular and cellular biology

authors： Sun S,Elwood J,Greene WC

更新日期：1996-03-01 00:00:00

abstract：:We report the cloning and functional analysis of a complete clone for the third member of the mouse mdr gene family, mdr3. Nucleotide and predicted amino acid sequence analyses showed that the three mouse mdr genes encode highly homologous membrane glycoproteins, which share the same length (1,276 residues), the same ...

journal_title：Molecular and cellular biology

authors： Devault A,Gros P

更新日期：1990-04-01 00:00:00

abstract：:Previously, we described a retrovirus vector that contained two genes: a 5' gene under transcriptional control of the viral long terminal repeat and a 3' gene under transcriptional control of the herpes simplex virus thymidine kinase promoter. By using a biological assay, we found that expression of the 5' gene is sup...

journal_title：Molecular and cellular biology

authors： Emerman M,Temin HM

更新日期：1986-03-01 00:00:00

abstract：:Selective transcription of the insulin gene in pancreatic beta cells is regulated by its enhancer, located within the 5'-flanking region of the insulin gene. Transcription from the enhancer is controlled by both positive- and negative-acting cellular transcription factors. It was previously shown that both the 243- an...

journal_title：Molecular and cellular biology

authors： Stein RW,Whelan J

更新日期：1989-10-01 00:00:00

abstract：:Previous studies have shown that expansion-prone repeats form structures that inhibit human flap endonuclease (FEN-1). We report here that faulty processing by FEN-1 initiates repeat instability in mammalian cells. Disease-length CAG tracts in Huntington's disease mice heterozygous for FEN-1 display a tendency toward ...

journal_title：Molecular and cellular biology

authors： Spiro C,McMurray CT

更新日期：2003-09-01 00:00:00

abstract：:The human immunodeficiency virus type 1 Rev protein contains a nuclear export signal (NES) that is required for Rev-mediated RNA export in mammals as well as in the yeast Saccharomyces cerevisiae. The Rev NES has been shown to specifically interact with a human (hRIP/RAB1) and a yeast (yRip1p) protein in the two-hybri...

journal_title：Molecular and cellular biology

authors： Stutz F,Izaurralde E,Mattaj IW,Rosbash M

更新日期：1996-12-01 00:00:00

abstract：:Cell lines established from the lepidopteran insect Spodoptera frugiperda (fall armyworm; Sf9) are used routinely as hosts for the expression of foreign proteins by recombinant baculovirus vectors. We have examined the pathway of protein glycosylation and secretion in these cells, using human tissue plasminogen activa...

journal_title：Molecular and cellular biology

authors： Jarvis DL,Summers MD

更新日期：1989-01-01 00:00:00

abstract：:Vascular endothelium (VE), the monolayer of endothelial cells that lines the vascular tree, undergoes damage at the basis of some vascular diseases. Its integrity is maintained by VE-cadherin, an adhesive receptor localized at cell-cell junctions. Here, we show that VE-cadherin is also located at the tip and along fil...

journal_title：Molecular and cellular biology

authors： Almagro S,Durmort C,Chervin-Pétinot A,Heyraud S,Dubois M,Lambert O,Maillefaud C,Hewat E,Schaal JP,Huber P,Gulino-Debrac D

更新日期：2010-04-01 00:00:00