Insulin gene enhancer activity is inhibited by adenovirus 5 E1a gene products.

abstract：:A key step in homologous recombination is the loading of Rad51 onto single-stranded DNA to form a nucleoprotein filament that promotes homologous DNA pairing and strand exchange. Mediator proteins, such as Rad52 and Rad55-Rad57, are thought to aid filament assembly by overcoming an inhibitory effect of the single-stra...

journal_title：Molecular and cellular biology

authors： Osman F,Dixon J,Barr AR,Whitby MC

更新日期：2005-09-01 00:00:00

abstract：:TIN2 is a negative regulator of telomere elongation that interacts with telomeric DNA repeat binding factor 1 (TRF1) and affects telomere length by a telomerase-dependent mechanism. Here we show that inactivation of the mouse TRF1-interacting protein 2 (TIN2) gene results in early embryonic lethality. We further obser...

journal_title：Molecular and cellular biology

authors： Chiang YJ,Kim SH,Tessarollo L,Campisi J,Hodes RJ

更新日期：2004-08-01 00:00:00

abstract：:Plasmids carrying the herpes simplex virus thymidine kinase (tk) gene were used to transfect thymidine kinase-deficient cells of the mouse fibroblast cell line LM(tk-). Individual cell clones were cultivated in selective hypoxanthine-aminopterin-thymidine medium to determine the number of integrated plasmid copies whi...

journal_title：Molecular and cellular biology

authors： Pülm W,Knippers R

更新日期：1985-02-01 00:00:00

abstract：:We have characterized the interactions between the TATA element and other sequence elements of a human heat shock protein 70 (hsp70) promoter by a mutational approach. Expression of a distal element of this promoter requires an intact TATA element in human cell lines. The hsp70 TATA element can be functionally replace...

journal_title：Molecular and cellular biology

authors： Greene JM,Kingston RE

更新日期：1990-04-01 00:00:00

abstract：:RSC is an essential chromatin remodeling complex in Saccharomyces cerevisiae that performs central roles in transcriptional regulation and cell cycle progression. Here we identify Htl1 as a novel factor that associates with the RSC complex both physically and functionally. We isolated HTL1 through a genetic screen for...

journal_title：Molecular and cellular biology

authors： Romeo MJ,Angus-Hill ML,Sobering AK,Kamada Y,Cairns BR,Levin DE

更新日期：2002-12-01 00:00:00

abstract：:Fibroblast growth factor 1 (FGF-1) is a powerful angiogenic factor whose gene structure contains four promoters, giving rise to a process of alternative splicing resulting in four mRNAs with alternative 5' untranslated regions (5' UTRs). Here we have identified, by using double luciferase bicistronic vectors, the pres...

journal_title：Molecular and cellular biology

authors： Martineau Y,Le Bec C,Monbrun L,Allo V,Chiu IM,Danos O,Moine H,Prats H,Prats AC

更新日期：2004-09-01 00:00:00

abstract：:The ribosomal proteins (RPs) of Saccharomyces cerevisiae are encoded by 137 genes that are among the most transcriptionally active in the genome. These genes are coordinately regulated: a shift up in temperature leads to a rapid, but temporary, decline in RP mRNA levels. A defect in any part of the secretory pathway l...

journal_title：Molecular and cellular biology

authors： Li B,Nierras CR,Warner JR

更新日期：1999-08-01 00:00:00

abstract：:We identified Ras guanine-releasing protein 3 (RasGRP3) as a guanine exchange factor expressed in blood vessels via an embryonic stem (ES) cell-based gene trap screen to identify novel vascular genes. RasGRP3 is expressed in embryonic blood vessels, down-regulated in mature adult vessels, and reexpressed in newly form...

journal_title：Molecular and cellular biology

authors： Roberts DM,Anderson AL,Hidaka M,Swetenburg RL,Patterson C,Stanford WL,Bautch VL

更新日期：2004-12-01 00:00:00

abstract：:Heterokaryons of Saccharomyces cerevisiae have been constructed utilizing the kar1-1 mutation, which prevents nuclear fusion during conjugation (J. Conde and G. Fink, Proc. Natl. Acad. Sci. U.S.A. 73:3651-3655, 1976). Each heterokaryon contained two haploid nuclei that were marked on several chromosomes. They segregat...

journal_title：Molecular and cellular biology

authors： Dutcher SK

更新日期：1981-03-01 00:00:00

abstract：:The expression of c-ets2 is rapidly induced in a variety of myelomonocytic cell lines as they differentiate into macrophages. We find that constitutive expression of c-ets2 in the M1D+ myeloblast leukemic cell line (M1ets2) is sufficient to push these cells to a more differentiated state. The expression of several dif...

journal_title：Molecular and cellular biology

authors： Aperlo C,Pognonec P,Stanley ER,Boulukos KE

更新日期：1996-12-01 00:00:00

abstract：:The opposing effects on proliferation mediated by G-protein-coupled receptor isoforms differing in their COOH termini could be correlated with the abilities of the receptors to differentially activate p38, implicated in apoptotic events, or phosphatidylinositol 3-kinase (PI 3-K), which provides a source of survival si...

journal_title：Molecular and cellular biology

authors： Sellers LA,Alderton F,Carruthers AM,Schindler M,Humphrey PP

更新日期：2000-08-01 00:00:00

abstract：:The natriuretic peptide receptors are three homologous cell surface proteins, each with a single transmembrane domain. The atrial natriuretic peptide receptor type A (ANPRA) and the homologous receptor type B (ANPRB) are both membrane guanylyl cyclases that synthesize cyclic GMP as an intracellular second messenger. T...

journal_title：Molecular and cellular biology

authors： Wilcox JN,Augustine A,Goeddel DV,Lowe DG

更新日期：1991-07-01 00:00:00

abstract：:The baker's yeast Saccharomyces cerevisiae expresses three homologues of the Nramp family of metal transporters: Smf1p, Smf2p, and Smf3p, encoded by SMF1, SMF2, and SMF3, respectively. Here we report a comparative analysis of the yeast Smf proteins at the levels of localization, regulation, and function of the corresp...

journal_title：Molecular and cellular biology

authors： Portnoy ME,Liu XF,Culotta VC

更新日期：2000-11-01 00:00:00

abstract：:Our analysis of rotund (rn) null mutations in Drosophila melanogaster revealed that deletion of the rn locus affects both spermatid and retinal differentiation. In the male reproductive system, the absence of RnRacGAP induced small testes, empty seminal vesicles, short testicular cysts, reduced amounts of interspermat...

journal_title：Molecular and cellular biology

authors： Bergeret E,Pignot-Paintrand I,Guichard A,Raymond K,Fauvarque MO,Cazemajor M,Griffin-Shea R

更新日期：2001-09-01 00:00:00

abstract：:Hox gene functions are intimately linked to correct developmental expression of the genes. The identification of cis-acting regulatory sequences and their associated trans-acting factors constitutes a key step in deciphering the mechanisms underlying the correct positioning of the functional domain of Hox genes along ...

journal_title：Molecular and cellular biology

authors： Tabariès S,Lapointe J,Besch T,Carter M,Woollard J,Tuggle CK,Jeannotte L

更新日期：2005-02-01 00:00:00

abstract：:The message for the zinc finger gene Rex-1 (Zfp-42) is expressed in undifferentiated murine F9 teratocarcinoma cells and embryonic stem cells. Expression of Rex-1 is reduced at the transcriptional level when F9 cells are induced by the addition of retinoic acid (RA) to differentiate. We have isolated genomic DNA for t...

journal_title：Molecular and cellular biology

authors： Hosler BA,Rogers MB,Kozak CA,Gudas LJ

更新日期：1993-05-01 00:00:00

abstract：:We examined the mechanisms that control the downregulation of the c-myc mRNA during differentiation of HL60 cells. On treatment with dimethyl sulfoxide, HL60 cells downmodulated their steady-state c-myc message levels, ceased to proliferate, and underwent terminal differentiation. In nuclear run-on assays in which dis...

journal_title：Molecular and cellular biology

authors： Siebenlist U,Bressler P,Kelly K

更新日期：1988-02-01 00:00:00

abstract：:Genetic and biochemical experiments have enabled us to more clearly distinguish three genetic loci, emtA, emtB, and emtC, all of which can be altered to give rise to resistance to the protein synthesis inhibitor, emetine, in cultured Chinese hamster cells. Genetic experiments have demonstrated that, unlike the emtB lo...

journal_title：Molecular and cellular biology

authors： Chang S,Wasmuth JJ

更新日期：1983-03-01 00:00:00

abstract：:The c-Jun NH2-terminal protein kinase (JNK) is a member of the mitogen-activated protein kinase (MAPK) group and is an essential component of a signaling cascade that is activated by exposure of cells to environmental stress. JNK activation is regulated by phosphorylation on both Thr and Tyr residues by a dual-specifi...

journal_title：Molecular and cellular biology

authors： Tournier C,Whitmarsh AJ,Cavanagh J,Barrett T,Davis RJ

更新日期：1999-02-01 00:00:00

abstract：:Intestinal alkaline phosphatase (IAP) is the most ancestral of the tissue-specific members of the AP gene family. Several studies have suggested an absorptive function for IAP, but in vivo data to this effect have been lacking. We inactivated the mouse IAP gene in embryo-derived stem cells and generated mice homozygou...

journal_title：Molecular and cellular biology

authors： Narisawa S,Huang L,Iwasaki A,Hasegawa H,Alpers DH,Millán JL

更新日期：2003-11-01 00:00:00

abstract：:Introduction of simian virus 40 T antigen into rodent fibroblasts gives rise to cells that can proliferate indefinitely but are dependent upon it for maintenance of their growth once the normal mitotic life span has elapsed. Inactivation of T antigen in these immortalized cells causes rapid and irreversible cessation ...

journal_title：Molecular and cellular biology

authors： Gonos ES,Burns JS,Mazars GR,Kobrna A,Riley TE,Barnett SC,Zafarana G,Ludwig RL,Ikram Z,Powell AJ,Jat PS

更新日期：1996-09-01 00:00:00

abstract：:The yeast alpha-factor pheromone receptor is a member of the G-protein-coupled receptor family. Limited trypsin digestion of yeast membranes was used to investigate ligand-induced conformational changes in this receptor. The agonist, alpha-factor, accelerated cleavage in the third intracellular loop, whereas the antag...

journal_title：Molecular and cellular biology

authors： Büküşoğlu G,Jenness DD

更新日期：1996-09-01 00:00:00

abstract：:The polycomb protein Bmi-1 represses the INK4a locus, which encodes the tumor suppressors p16 and p14(ARF). Here we report that Bmi-1 is downregulated when WI-38 human fibroblasts undergo replicative senescence, but not quiescence, and extends replicative life span when overexpressed. Life span extension by Bmi-1 requ...

journal_title：Molecular and cellular biology

authors： Itahana K,Zou Y,Itahana Y,Martinez JL,Beausejour C,Jacobs JJ,Van Lohuizen M,Band V,Campisi J,Dimri GP

更新日期：2003-01-01 00:00:00

abstract：:Ribosomal protein L5 binds specifically to 5S rRNA to form a complex that is a precursor to 60S subunit assembly in vivo. Analyses in yeast cells, mammalian cells, and Xenopus embryos have shown that the accumulation of L5 is not coordinated with the expression of other ribosomal proteins. In this study, the primary s...

journal_title：Molecular and cellular biology

authors： Wormington WM

更新日期：1989-12-01 00:00:00

abstract：:Imprinted genes are expressed in a monoallelic, parent-of-origin-specific manner. Clusters of imprinted genes are regulated by imprinting control regions (ICRs) characterized by DNA methylation of one allele. This methylation is critical for imprinting; a reduction in the DNA methyltransferase DNMT1 causes a widesprea...

journal_title：Molecular and cellular biology

authors： Weaver JR,Sarkisian G,Krapp C,Mager J,Mann MR,Bartolomei MS

更新日期：2010-08-01 00:00:00

abstract：:As part of an effort to characterize the spatial and functional relationships among genetic elements within the amplified dihydrofolate reductase (DHFR) domain in Chinese hamster cells, we have used a variation of the differential hybridization approach to identify cDNA clones whose genes are coamplified with DHFR in ...

journal_title：Molecular and cellular biology

authors： Foreman PK,Hamlin JL

更新日期：1989-03-01 00:00:00

abstract：:Spermatogonial stem cells (SSCs) are unipotent germ cells that are at the foundation of spermatogenesis and male fertility. However, the underlying molecular mechanisms governing SSC stemness and growth properties remain elusive. We have recently identified chromodomain helicase/ATPase DNA binding protein 1-like (Chd1...

journal_title：Molecular and cellular biology

authors： Liu SS,Maguire EM,Bai YS,Huang L,Liu Y,Xu L,Fauzi I,Zhang SQ,Xiao Q,Ma NF

更新日期：2019-02-04 00:00:00

abstract：:The carboxyl-terminal domain (CTD) of RNA polymerase II (Pol II) can be phosphorylated at serine 2 (Ser-2) and serine 5 (Ser-5) of the CTD heptad repeat YSPTSPS, and this phosphorylation is important in coupling transcription to RNA processing, including 5' capping, splicing, and polyadenylation. The mammalian endogen...

journal_title：Molecular and cellular biology

authors： Cheng C,Sharp PA

更新日期：2003-03-01 00:00:00

abstract：:Three subtypes of retinoic acid receptors (RAR), termed RAR alpha, RAR beta, and RAR gamma, have been described. They are composed of different structural domains, including distinct domains for DNA and ligand binding. RARs specifically bind all-trans-retinoic acid (RA), 9-cis-RA, and retinoid analogs. In this study, ...

journal_title：Molecular and cellular biology

authors： Lamour FP,Lardelli P,Apfel CM

更新日期：1996-10-01 00:00:00

abstract：:We have developed a high-frequency cotransformation system which is useful in introducing nonreplicating circular DNA plasmids into the fission yeast Schizosaccharomyces pombe. This system depends on two factors: the ability of the ural-complementing helper plasmids pFYM2 and pFYM225 to propagate autonomously in S. po...

journal_title：Molecular and cellular biology

authors： Sakai K,Sakaguchi J,Yamamoto M

更新日期：1984-04-01 00:00:00