Abstract:
:We analyzed sites of macronuclear telomere addition at a single genetic locus in Paramecium tetraurelia. We showed that in homozygous wild-type cells, differential genomic processing during macronuclear development resulted in the A surface antigen gene being located 8, 13, or 26 kilobases upstream from a macronuclear telomere. We describe variable rearrangements that occurred at the telomere 8 kilobases from the A gene. A mutant (d48) that forms a telomere near the 5' end of the A gene was also analyzed. This mutant was shown to create simple terminal deletions; telomeric repeats were added directly to the truncated wild-type A gene sequence. In both the mutant and wild-type cells, the telomeric sequences (a mixture of C4A2 and C3A3 repeats) were added to various sequences within a specific 200- to 500-base-pair region rather than to a single site. No similarities were found in the primary sequences surrounding the telomere addition sites. The mutation in d48 changed the region of telomere addition at the A gene locus; this is the first example in ciliates of a mutation that affects the site of telomere addition.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Forney JD,Blackburn EHdoi
10.1128/mcb.8.1.251subject
Has Abstractpub_date
1988-01-01 00:00:00pages
251-8issue
1eissn
0270-7306issn
1098-5549journal_volume
8pub_type
杂志文章abstract::Heterochromatin protein 1 (HP1) family members are versatile proteins involved in transcription, chromatin organization, and replication. Recent findings now have implicated HP1 proteins in the DNA damage response as well. Cell-biological approaches showed that reducing the levels of all three HP1 isoforms enhances DN...
journal_title:Molecular and cellular biology
pub_type: 杂志文章,评审
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journal_title:Molecular and cellular biology
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:1994-08-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:1996-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/mcb.13.4.2286
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pub_type: 杂志文章
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更新日期:1993-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1990-06-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2002-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/MCB.00131-10
更新日期:2010-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2013-08-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2010-11-01 00:00:00
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pub_type: 杂志文章
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:2005-11-01 00:00:00
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pub_type: 杂志文章
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