Formation of tRNA Wobble Inosine in Humans Is Disrupted by a Millennia-Old Mutation Causing Intellectual Disability.

abstract：:Fragments of 5'-flanking and noncoding exon I sequences of the human gastrin gene were analyzed in transient expression assays after transfection of a variety of cell lines with the pSVCAT vector system. In the presence of the simian virus 40 (SV40) enhancer, the gastrin gene fragment from nucleotides -250 to +57, rel...

journal_title：Molecular and cellular biology

authors： Theill LE,Wiborg O,Vuust J

更新日期：1987-12-01 00:00:00

abstract：:Thyroid hormone (T3) is critical for growth, differentiation, and maintenance of metabolic homeostasis. Mice with a knock-in mutation in the thyroid hormone receptor alpha gene (TRalpha1PV) were created previously to explore the roles of mutated TRalpha1 in vivo. TRalpha1PV is a dominant negative mutant with a framesh...

journal_title：Molecular and cellular biology

authors： Ying H,Araki O,Furuya F,Kato Y,Cheng SY

更新日期：2007-03-01 00:00:00

abstract：:Heat shock proteins (HSPs) are highly conserved among all organisms from prokaryotes to eukaryotes. In mice, the HSP genes Hsp70.1 and Hsp70.3 are induced by both endogenous and exogenous stressors, such as heat and toxicants. In order to determine whether such proteins specifically influence genomic instability, mice...

journal_title：Molecular and cellular biology

authors： Hunt CR,Dix DJ,Sharma GG,Pandita RK,Gupta A,Funk M,Pandita TK

更新日期：2004-01-01 00:00:00

abstract：:Nuclear receptor corepressors (N-CoR) and silencing mediator for retinoid and thyroid receptors (SMRT) have both been implicated in thyroid hormone receptor (TR)-mediated repression. Here we show that endogenous N-CoR, TBL1, and histone deacetylase 3 (HDAC3), but not HDAC1, -2, or -4, are recruited to a stably integra...

journal_title：Molecular and cellular biology

authors： Ishizuka T,Lazar MA

更新日期：2003-08-01 00:00:00

abstract：:Ecdysoneless (ECD) is an evolutionarily conserved protein whose germ line deletion is embryonic lethal. Deletion of Ecd in cells causes cell cycle arrest, which is rescued by exogenous ECD, demonstrating a requirement of ECD for normal mammalian cell cycle progression. However, the exact mechanism by which ECD regulat...

journal_title：Molecular and cellular biology

authors： Mir RA,Bele A,Mirza S,Srivastava S,Olou AA,Ammons SA,Kim JH,Gurumurthy CB,Qiu F,Band H,Band V

更新日期：2015-12-28 00:00:00

abstract：:We employed a protein gel blotting procedure to search for nuclear proteins from rat pituitary cells that bind preferentially to the 5'-flanking region of the rat prolactin gene. By gel blots of chromatin proteins from GH3 rat pituitary tumor cells with a 32P-labeled prolactin genomic clone, we detected two major bind...

journal_title：Molecular and cellular biology

authors： White BA,Preston GM,Lufkin TC,Bancroft C

更新日期：1985-11-01 00:00:00

abstract：:We have characterized the interactions between the TATA element and other sequence elements of a human heat shock protein 70 (hsp70) promoter by a mutational approach. Expression of a distal element of this promoter requires an intact TATA element in human cell lines. The hsp70 TATA element can be functionally replace...

journal_title：Molecular and cellular biology

authors： Greene JM,Kingston RE

更新日期：1990-04-01 00:00:00

abstract：:Clearance of apoptotic cells by phagocytic neighbors is crucial for normal development of multicellular organisms. However, how phagocytes discriminate between healthy and dying cells remains poorly understood. We focus on glial phagocytosis of apoptotic neurons during development of the Drosophila central nervous sys...

journal_title：Molecular and cellular biology

authors： Shklyar B,Levy-Adam F,Mishnaevski K,Kurant E

更新日期：2013-08-01 00:00:00

abstract：:We have exploited the Escherichia coli lac operator/repressor system as a means to regulate the expression of a mammalian tRNA gene in vivo and in vitro. An oligonucleotide containing a lac operator (lacO) site was cloned immediately upstream of a human serine amber suppressor (Su+) tRNA gene. Insertion of a single la...

journal_title：Molecular and cellular biology

authors： Syroid DE,Tapping RI,Capone JP

更新日期：1992-10-01 00:00:00

abstract：:Max is a basic helix-loop-helix/leucine zipper protein that forms heterodimers with the Myc family of proteins to promote cell growth and with the Mad/Mxi1 family of proteins to inhibit cell growth. The role of Max as the obligate binding partner for these two protein families necessitates the observed constitutive ex...

journal_title：Molecular and cellular biology

authors： Peters MA,Sollenberger KG,Kao TL,Taparowsky EJ

更新日期：1997-03-01 00:00:00

abstract：:Histone H1 is an abundant and essential component of chromatin whose precise role in regulating gene expression is poorly understood. Here, we report that a major target of H1-mediated regulation in embryonic stem (ES) cells is the X-linked Rhox homeobox gene cluster. To address the underlying mechanism, we examined t...

journal_title：Molecular and cellular biology

authors： Maclean JA,Bettegowda A,Kim BJ,Lou CH,Yang SM,Bhardwaj A,Shanker S,Hu Z,Fan Y,Eckardt S,McLaughlin KJ,Skoultchi AI,Wilkinson MF

更新日期：2011-03-01 00:00:00

abstract：:Polycomb (Pc) is part of a Pc group (PcG) protein complex that is involved in repression of gene activity during Drosophila and vertebrate development. To identify proteins that interact with vertebrate Pc homologs, we performed two-hybrid screens with Xenopus Pc (XPc) and human Pc 2 (HPC2). We find that the C-termina...

journal_title：Molecular and cellular biology

authors： Sewalt RG,Gunster MJ,van der Vlag J,Satijn DP,Otte AP

更新日期：1999-01-01 00:00:00

abstract：:Phosphorylation of eukaryotic initiation factor 2α (eIF2α) controls transcriptome-wide changes in mRNA translation in stressed cells. While phosphorylated eIF2α (P-eIF2α) attenuates global protein synthesis, mRNAs encoding stress proteins are more efficiently translated. Two eIF2α phosphatases, containing GADD34 and C...

journal_title：Molecular and cellular biology

authors： Reid DW,Tay AS,Sundaram JR,Lee IC,Chen Q,George SE,Nicchitta CV,Shenolikar S

更新日期：2016-06-15 00:00:00

abstract：:The Cockayne syndrome B protein (CSB) is required for coupling DNA excision repair to transcription in a process known as transcription-coupled repair (TCR). Cockayne syndrome patients show UV sensitivity and severe neurodevelopmental abnormalities. CSB is a DNA-dependent ATPase of the SWI2/SNF2 family. SWI2/SNF2-like...

journal_title：Molecular and cellular biology

authors： Citterio E,Van Den Boom V,Schnitzler G,Kanaar R,Bonte E,Kingston RE,Hoeijmakers JH,Vermeulen W

更新日期：2000-10-01 00:00:00

abstract：:The hexanucleotide AAUAAA has been demonstrated to be part of the signal for cleavage and polyadenylation at appropriate sites on eucaryotic mRNA precursors. Since this sequence is not unique to polyadenylation sites, it cannot be the entire signal for the cleavage event. We have extended the definition of the polyade...

journal_title：Molecular and cellular biology

authors： Sadofsky M,Alwine JC

更新日期：1984-08-01 00:00:00

abstract：:Oncogenic activation of the mitogen-activated protein (MAP) kinase cascade in murine fibroblasts initiates a senescence-like cell cycle arrest that depends on the ARF/p53 tumor suppressor pathway. To investigate whether p53 is sufficient to induce senescence, we introduced a conditional murine p53 allele (p53(val135))...

journal_title：Molecular and cellular biology

authors： Ferbeyre G,de Stanchina E,Lin AW,Querido E,McCurrach ME,Hannon GJ,Lowe SW

更新日期：2002-05-01 00:00:00

abstract：:Saccharomyces cerevisiae mutant E124 was selected in a visual screen based on elongated cell shape. Genetic analysis showed that E124 contains two separate mutations, pps1-1 and elm4-1, each causing a distinct phenotype inherited as a single-gene trait. In rich medium, pps1-1 by itself causes increased doubling time b...

journal_title：Molecular and cellular biology

authors： Blacketer MJ,Madaule P,Myers AM

更新日期：1994-07-01 00:00:00

abstract：:There are five members of the RFX family of transcription factors in mammals. While RFX5 plays a well-defined role in the immune system, the functions of RFX1 to RFX4 remain largely unknown. We have generated mice with a deletion of the Rfx3 gene. RFX3-deficient mice exhibit frequent left-right (LR) asymmetry defects ...

journal_title：Molecular and cellular biology

authors： Bonnafe E,Touka M,AitLounis A,Baas D,Barras E,Ucla C,Moreau A,Flamant F,Dubruille R,Couble P,Collignon J,Durand B,Reith W

更新日期：2004-05-01 00:00:00

abstract：:There exist in the Xenopus laevis genome clusters of tandemly repeated DNA sequences, consisting of two types of 393-base-pair repeating unit. Each such cluster contains several units of one of these paired tandem repeats (PTR-1), followed by several units of the other repeat (PTR-2). The number of repeats of each typ...

journal_title：Molecular and cellular biology

authors： Carroll D,Garrett JE,Lam BS

更新日期：1984-02-01 00:00:00

abstract：:Prdm1/Blimp-1 is a master regulator of gene expression in diverse tissues of the developing embryo and adult organism. Its C-terminal zinc finger domain mediates nuclear import, DNA binding, and recruitment of the corepressors G9a and HDAC1/2. Alternatively spliced transcripts lacking exon 7 sequences encode a structu...

journal_title：Molecular and cellular biology

authors： Morgan MA,Mould AW,Li L,Robertson EJ,Bikoff EK

更新日期：2012-09-01 00:00:00

abstract：:An interferon-alpha-like sequence was isolated from a human genomic library by hybridization with a 15-base oligonucleotide. The sequence also showed homology to alpha-interferon and was most closely related to the leukocyte interferon-M gene fragment. The original isolate cross-hybridized to a family of sequences, 10...

journal_title：Molecular and cellular biology

authors： Feinstein SI,Mory Y,Chernajovsky Y,Maroteaux L,Nir U,Lavie V,Revel M

更新日期：1985-03-01 00:00:00

abstract：:We have screened an adult rat cerebellar cDNA library in search of novel protein tyrosine-kinase (PTK) cDNAs. A cDNA for a putative PTK, trkB, was cloned, and its sequence indicates that it is likely to be derived from a gene for a ligand-regulated receptor closely related to the human trk oncogene. Northern (RNA) ana...

journal_title：Molecular and cellular biology

authors： Middlemas DS,Lindberg RA,Hunter T

更新日期：1991-01-01 00:00:00

abstract：:The expressed variant cell surface glycoprotein (VSG) gene of the protozoan parasite Trypanosoma brucei is invariably found at one of several telomeric VSG gene expression sites (ESs). The active ES in variant 118 clone 1 is found on a 1.5-Mb chromosome, and the promoter region is located more than 45 kb upstream of t...

journal_title：Molecular and cellular biology

authors： Gottesdiener KM,Goriparthi L,Masucci JP,Van der Ploeg LH

更新日期：1992-10-01 00:00:00

abstract：:We have previously shown that activation of extracellular signal-regulated kinase (Erk) by epidermal growth factor (EGF) treatment was significantly decreased in mouse fibroblast cells expressing a mutant Shp-2 molecule lacking 65 amino acids in the SH2-N domain, Shp-2(Delta46-110). To address the molecular mechanism ...

journal_title：Molecular and cellular biology

authors： Shi ZQ,Yu DH,Park M,Marshall M,Feng GS

更新日期：2000-03-01 00:00:00

abstract：:Structural maintenance of chromosomes (SMC) complexes and DNA topoisomerases are major determinants of chromosome structure and dynamics. The cohesin complex embraces sister chromatids throughout interphase, but during mitosis most cohesin is stripped from chromosome arms by early prophase, while the remaining cohesin...

journal_title：Molecular and cellular biology

authors： Tapia-Alveal C,Lin SJ,Yeoh A,Jabado OJ,O'Connell MJ

更新日期：2014-06-01 00:00:00

abstract：:Splicing of tRNA precursors in Saccharomyces cerevisiae extracts proceeds in two steps; excision of the intervening sequence and ligation of the tRNA halves. The ability to resolve these two steps and the distinct physical properties of the endonuclease and ligase suggested that the splicing steps may not be concerted...

journal_title：Molecular and cellular biology

authors： Greer CL

更新日期：1986-02-01 00:00:00

abstract：:We investigated the interaction of phospholipase C-gamma (PLC-gamma) with wild-type and mutant forms of the platelet-derived growth factor (PDGF) beta-receptor both in vivo and in vitro. After PDGF treatment of CHO cell lines expressing wild-type or either of two mutant (delta Ki and Y825F) PDGF receptors, PLC-gamma b...

journal_title：Molecular and cellular biology

authors： Morrison DK,Kaplan DR,Rhee SG,Williams LT

更新日期：1990-05-01 00:00:00

abstract：:Smad6 and Smad7 are inhibitory Smads induced by transforming growth factor beta-Smad signal transduction pathways in a negative-feedback mechanism. Previously it has been thought that inhibitory Smads bind to the type I receptor and block the phosphorylation of receptor-activated Smads, thereby inhibiting the initiati...

journal_title：Molecular and cellular biology

authors： Lin X,Liang YY,Sun B,Liang M,Shi Y,Brunicardi FC,Shi Y,Feng XH

更新日期：2003-12-01 00:00:00

abstract：:Exchange proteins activated by cAMP (cyclic AMP) 2 (Epac2) is a guanine nucleotide exchange factor for Rap1, a small G protein involved in many cellular functions, including cell adhesion, differentiation, and exocytosis. Epac2 interacts with Ras-GTP via a Ras association (RA) domain. Previous studies have suggested t...

journal_title：Molecular and cellular biology

authors： Liu C,Takahashi M,Li Y,Song S,Dillon TJ,Shinde U,Stork PJ

更新日期：2008-12-01 00:00:00

abstract：:The mechanism by which the maize autonomous Ac transposable element gives rise to nonautonomous Ds elements is largely unknown. Sequence analysis of native maize Ds elements indicates a complex chimeric structure formed through deletions of Ac sequences with or without insertions of Ac-unrelated sequence blocks. These...

journal_title：Molecular and cellular biology

authors： Rubin E,Levy AA

更新日期：1997-11-01 00:00:00