Enhanced translation and increased turnover of c-myc proteins occur during differentiation of murine erythroleukemia cells.

abstract：:Degenerate oligonucleotide probes corresponding to a highly conserved region common to epidermal growth factor, transforming growth factor alpha, and vaccinia growth factor were used to identify a novel growth factor gene in the Shope fibroma virus genome. Sequence analysis indicates that the Shope fibroma growth fact...

journal_title：Molecular and cellular biology

authors： Chang W,Upton C,Hu SL,Purchio AF,McFadden G

更新日期：1987-01-01 00:00:00

abstract：:Determining the roles of Rel/NF-kappaB transcription factors in mouse skin development with loss-of-function mutants has been limited by redundancy among these proteins and by embryonic lethality associated with the absence of RelA. Using mice lacking RelA and c-rel, which survive throughout embryogenesis on a tumor n...

journal_title：Molecular and cellular biology

authors： Gugasyan R,Voss A,Varigos G,Thomas T,Grumont RJ,Kaur P,Grigoriadis G,Gerondakis S

更新日期：2004-07-01 00:00:00

abstract：:Appropriate cell cycle checkpoint control is essential for the maintenance of cell and organismal homeostasis. Members of the Nek (NIMA-related kinase) family of serine/threonine protein kinases have been implicated in the regulation of various aspects of the cell cycle. We explored the cellular functions of Nek10, a ...

journal_title：Molecular and cellular biology

authors： Moniz LS,Stambolic V

更新日期：2011-01-01 00:00:00

abstract：:The RBP-J/Su(H) DNA-binding protein plays a key role in transcriptional regulation by targeting Epstein-Barr virus nuclear antigen 2 (EBNA2) and the intracellular portions of Notch receptors to specific promoters. Using the yeast two-hybrid system, we isolated a LIM-only protein, KyoT, which physically interacts with ...

journal_title：Molecular and cellular biology

authors： Taniguchi Y,Furukawa T,Tun T,Han H,Honjo T

更新日期：1998-01-01 00:00:00

abstract：:Mutations in the Drosophila mus308 gene confer specific hypersensitivity to DNA-cross-linking agents as a consequence of defects in DNA repair. The mus308 gene is shown here to encode a 229-kDa protein in which the amino-terminal domain contains the seven conserved motifs characteristic of DNA and RNA helicases and th...

journal_title：Molecular and cellular biology

authors： Harris PV,Mazina OM,Leonhardt EA,Case RB,Boyd JB,Burtis KC

更新日期：1996-10-01 00:00:00

abstract：:In in vitro splicing reactions, influenza virus NS1 mRNA was not detectably spliced, but nonetheless very efficiently formed ATP-dependent 55S complexes containing the U1, U2, U4, U5, and U6 small nuclear ribonucleoproteins (snRNPs) (C. H. Agris, M. E. Nemeroff, and R. M. Krug, Mol. Cell. Biol. 9:259-267, 1989). We de...

journal_title：Molecular and cellular biology

authors： Nemeroff ME,Utans U,Krämer A,Krug RM

更新日期：1992-03-01 00:00:00

abstract：:The mitochondrial genome of trypanosomes is composed of thousands of topologically interlocked circular DNA molecules that form the kinetoplast DNA (kDNA). Most genes encoded by the kDNA require a posttranscriptional modification process called RNA editing to form functional mRNAs. Here, we show that alternative editi...

journal_title：Molecular and cellular biology

authors： Ochsenreiter T,Anderson S,Wood ZA,Hajduk SL

更新日期：2008-09-01 00:00:00

abstract：:In addition to the highly conserved AATAAA sequence, there is a requirement for specific sequences downstream of polyadenylic acid [poly(A)] cleavage sites to generate correct mRNA 3' termini. Previous experiments demonstrated that 35 nucleotides downstream of the E2A poly(A) site were sufficient but 20 nucleotides we...

journal_title：Molecular and cellular biology

authors： Hart RP,McDevitt MA,Ali H,Nevins JR

更新日期：1985-11-01 00:00:00

abstract：:Secretory proteins in eukaryotic cells are transported to the cell surface via the endoplasmic reticulum (ER) and the Golgi apparatus by membrane-bounded vesicles. We screened a collection of temperature-sensitive mutants of Saccharomyces cerevisiae for defects in ER-to-Golgi transport. Two of the genes identified in ...

journal_title：Molecular and cellular biology

authors： Chen EJ,Frand AR,Chitouras E,Kaiser CA

更新日期：1998-12-01 00:00:00

abstract：:Very little is known about the mechanisms mediating longevities of mRNAs. As a means of identifying potential cis- and trans-acting elements which stabilize an individual mRNA, naturally occurring mutations that decreased stability of the normally long-lived globin mRNA were analyzed. Our previous studies demonstrated...

journal_title：Molecular and cellular biology

authors： Weiss IM,Liebhaber SA

更新日期：1995-05-01 00:00:00

abstract：:Recognition of gene promoters by RNA polymerase II is mediated by general transcription factor IID (TFIID), which has been thought to be a static complex and to play a passive role in the regulation of gene expression under the instruction of gene-specific transcription factors. Here we show that transforming growth f...

journal_title：Molecular and cellular biology

authors： Nakagawa T,Hosogane M,Nakagawa M,Morohoshi A,Funayama R,Nakayama K

更新日期：2018-02-12 00:00:00

abstract：:Up to 70% of human genes are associated with regions of nonmethylated DNA called CpG islands (S. Saxonov, P. Berg, and D. L. Brutlag, Proc. Natl. Acad. Sci. U. S. A. 103:1412-1417, 2006). Usually associated with the 5' end of genes, CpG islands are thought to impact gene expression. We previously demonstrated that the...

journal_title：Molecular and cellular biology

authors： Zhou JC,Blackledge NP,Farcas AM,Klose RJ

更新日期：2012-01-01 00:00:00

abstract：:The cytosolic heat shock cognate 70-kDa protein (hsc70) is required for efficient import of ornithine transcarbamylase precursor (pOTC) into rat liver mitochondria (K. Terada, K. Ohtsuka, N. Imamoto, Y. Yoneda, and M. Mori, Mol. Cell. Biol. 15:3708-3713, 1995). The requirement of hsc70 for mitochondrial import of vari...

journal_title：Molecular and cellular biology

authors： Terada K,Ueda I,Ohtsuka K,Oda T,Ichiyama A,Mori M

更新日期：1996-11-01 00:00:00

abstract：:Retrovirus vector infection was used to introduce large numbers of unique genetic markers into tumor cell populations for the purpose of analyzing comparative changes in the clonal composition of metastatic versus that of nonmetastatic tumors during their progressive growth in vivo. The cell lines used were SP1, a non...

journal_title：Molecular and cellular biology

authors： Korczak B,Robson IB,Lamarche C,Bernstein A,Kerbel RS

更新日期：1988-08-01 00:00:00

abstract：:Activation-induced deaminase (AID) is essential for class switch recombination and somatic hypermutation, and it has the ability to deaminate single-stranded DNA at cytidines. Mammalian class switch regions form R-loops upon transcription in the physiological orientation. The displaced DNA strand of an R-loop is force...

journal_title：Molecular and cellular biology

authors： Yu K,Roy D,Bayramyan M,Haworth IS,Lieber MR

更新日期：2005-03-01 00:00:00

abstract：:A pool of Candida albicans RsaI fragments cloned onto a vector containing pBR322 sequences and the Candida ADE2 gene was used to transform a Candida ade2 mutant to adenine protrophy. A potential autonomously replicating sequence (ARS) in Candida DNA was identified by two criteria: instability of the selectable marker ...

journal_title：Molecular and cellular biology

authors： Kurtz MB,Cortelyou MW,Miller SM,Lai M,Kirsch DR

更新日期：1987-01-01 00:00:00

abstract：:Bone metastasis is the hallmark of progressive and castration-resistant prostate cancers. MicroRNA 1 (miR-1) levels are decreased in clinical samples of primary prostate cancer and further reduced in metastases. SRC has been implicated as a critical factor in bone metastasis, and here we show that SRC is a direct targ...

journal_title：Molecular and cellular biology

authors： Liu YN,Yin J,Barrett B,Sheppard-Tillman H,Li D,Casey OM,Fang L,Hynes PG,Ameri AH,Kelly K

更新日期：2015-06-01 00:00:00

abstract：:The natriuretic peptide receptors are three homologous cell surface proteins, each with a single transmembrane domain. The atrial natriuretic peptide receptor type A (ANPRA) and the homologous receptor type B (ANPRB) are both membrane guanylyl cyclases that synthesize cyclic GMP as an intracellular second messenger. T...

journal_title：Molecular and cellular biology

authors： Wilcox JN,Augustine A,Goeddel DV,Lowe DG

更新日期：1991-07-01 00:00:00

abstract：:During V(D)J recombination, the coding ends to be joined are extensively modified. Those modifications, termed coding-end processing, consist of removal and addition of various numbers of nucleotides. We previously showed in vivo that coding-end processing is specific for each coding end, suggesting that specific moti...

journal_title：Molecular and cellular biology

authors： Nadel B,Feeney AJ

更新日期：1997-07-01 00:00:00

abstract：:ADAR2 is a double-stranded-RNA-specific adenosine deaminase involved in the editing of mammalian RNAs by the site-selective conversion of adenosine to inosine. Previous studies from our laboratory have demonstrated that ADAR2 can modify its own pre-mRNA to create a proximal 3' splice site containing a noncanonical ade...

journal_title：Molecular and cellular biology

authors： Feng Y,Sansam CL,Singh M,Emeson RB

更新日期：2006-01-01 00:00:00

abstract：:We have designed and constructed integration-defective retroviral vectors to explore their potential for gene targeting in mammalian cells. Two nonoverlapping deletion mutants of the bacterial neomycin resistance (neo) gene were used to detect homologous recombination events between viral and chromosomal sequences. St...

journal_title：Molecular and cellular biology

authors： Ellis J,Bernstein A

更新日期：1989-04-01 00:00:00

abstract：:TFIIIC plays a key role in nucleating the assembly of the initiation factor TFIIIB on class III genes. We have characterized an essential gene, TFC8, encoding the 60-kDa polypeptide, tau60, present in affinity-purified TFIIIC. Hemagglutinin-tagged variants of tau60 were found to be part of TFIIIC-tDNA complexes and to...

journal_title：Molecular and cellular biology

authors： Deprez E,Arrebola R,Conesa C,Sentenac A

更新日期：1999-12-01 00:00:00

abstract：:RAG1 and RAG2 catalyze the first DNA cleavage steps in V(D)J recombination. We demonstrate that the isolated central domain of RAG1 has inherent single-stranded (ss) DNA cleavage activity, which does not require, but is enhanced by, RAG2. The central domain, therefore, contains the active-site residues necessary to pe...

journal_title：Molecular and cellular biology

authors： De P,Peak MM,Rodgers KK

更新日期：2004-08-01 00:00:00

abstract：:GCN5, a putative transcriptional adapter in humans and yeast, possesses histone acetyltransferase (HAT) activity which has been linked to GCN5's role in transcriptional activation in yeast. In this report, we demonstrate a functional interaction between human GCN5 (hGCN5) and the DNA-dependent protein kinase (DNA-PK) ...

journal_title：Molecular and cellular biology

authors： Barlev NA,Poltoratsky V,Owen-Hughes T,Ying C,Liu L,Workman JL,Berger SL

更新日期：1998-03-01 00:00:00

abstract：:The laminin alpha4 chain, a component of laminin-8 and -9, is expressed in basement membranes, such as those beneath endothelia, the perineurium of peripheral nerves, and around developing muscle fibers. Laminin alpha4-null mice presented with hemorrhages during the embryonic and neonatal period and had extensive blee...

journal_title：Molecular and cellular biology

authors： Thyboll J,Kortesmaa J,Cao R,Soininen R,Wang L,Iivanainen A,Sorokin L,Risling M,Cao Y,Tryggvason K

更新日期：2002-02-01 00:00:00

abstract：:The pancreatic-islet-enriched transcription factors MafA and MafB have unique expression patterns in β cells in rodents. MafA is specifically expressed in β cells and is a key regulatory factor for maintaining adult β-cell function, whereas MafB plays an essential role in β-cell development during embryogenesis, and i...

journal_title：Molecular and cellular biology

authors： Xiafukaiti G,Maimaiti S,Ogata K,Kuno A,Kudo T,Shawki HH,Oishi H,Takahashi S

更新日期：2019-08-12 00:00:00

abstract：:An elastase-human growth hormone (hGH) fusion gene containing 205 base pairs of elastase 5' flanking region is expressed exclusively in pancreatic acinar cells of transgenic mice. This paper shows that the promoter region (-72 to +8) and the enhancer (-205 to -73) function independently of each other. The elastase enh...

journal_title：Molecular and cellular biology

authors： Ornitz DM,Hammer RE,Davison BL,Brinster RL,Palmiter RD

更新日期：1987-10-01 00:00:00

abstract：:We have previously reported that immobilized p21ras forms a GMPPNP-dependent complex with a MEK activity. Furthermore, the association of the MEK activity was found to be independent of the presence of Raf-1. We have extended those observations to show that MEK1 is the MEK activity previously described to associate wi...

journal_title：Molecular and cellular biology

authors： Moodie SA,Paris MJ,Kolch W,Wolfman A

更新日期：1994-11-01 00:00:00

abstract：:Inactivation of the tumor suppressor p53 by degradation is a mechanism utilized by cells to adapt to endoplasmic reticulum (ER) stress. However, the mechanisms of p53 destabilization by ER stress are not known. We demonstrate here that the E3 ubiquitin-ligase Hdm2 is essential for the nucleocytoplasmic transport and p...

journal_title：Molecular and cellular biology

authors： Pluquet O,Qu LK,Baltzis D,Koromilas AE

更新日期：2005-11-01 00:00:00

abstract：:Repair of chromosomal breaks is essential for cellular viability, but misrepair generates mutations and gross chromosomal rearrangements. We investigated the interrelationship between two homologous-repair pathways, i.e., mutagenic single-strand annealing (SSA) and precise homology-directed repair (HDR). For this, we ...

journal_title：Molecular and cellular biology

authors： Stark JM,Pierce AJ,Oh J,Pastink A,Jasin M

更新日期：2004-11-01 00:00:00