Gene targeting with retroviral vectors: recombination by gene conversion into regions of nonhomology.

abstract：:Posttranscriptional and posttranslational modification of macromolecules is known to fine-tune their functions. Trm112 is unique, acting as an activator of both tRNA and protein methyltransferases. Here we report that in Saccharomyces cerevisiae, Trm112 is required for efficient ribosome synthesis and progression thro...

journal_title：Molecular and cellular biology

authors： Figaro S,Wacheul L,Schillewaert S,Graille M,Huvelle E,Mongeard R,Zorbas C,Lafontaine DL,Heurgué-Hamard V

更新日期：2012-06-01 00:00:00

abstract：:Spermatogonial stem cells (SSCs) are unipotent germ cells that are at the foundation of spermatogenesis and male fertility. However, the underlying molecular mechanisms governing SSC stemness and growth properties remain elusive. We have recently identified chromodomain helicase/ATPase DNA binding protein 1-like (Chd1...

journal_title：Molecular and cellular biology

authors： Liu SS,Maguire EM,Bai YS,Huang L,Liu Y,Xu L,Fauzi I,Zhang SQ,Xiao Q,Ma NF

更新日期：2019-02-04 00:00:00

abstract：:The BamHI restriction enzyme mediates integration of nonhomologous DNA into the Saccharomyces cerevisiae genome (R. H. Schiestl and T. D. Petes, Proc. Natl. Acad. Sci. USA 88:7585-7589, 1991). The present study investigates the mechanism of such events: in particular, the mediating activity of various restriction enzy...

journal_title：Molecular and cellular biology

authors： Manivasakam P,Schiestl RH

更新日期：1998-03-01 00:00:00

abstract：:Three subtypes of retinoic acid receptors (RAR), termed RAR alpha, RAR beta, and RAR gamma, have been described. They are composed of different structural domains, including distinct domains for DNA and ligand binding. RARs specifically bind all-trans-retinoic acid (RA), 9-cis-RA, and retinoid analogs. In this study, ...

journal_title：Molecular and cellular biology

authors： Lamour FP,Lardelli P,Apfel CM

更新日期：1996-10-01 00:00:00

abstract：:Transposons have contributed protein coding sequences to a unexpectedly large number of human genes. Except for the V(D)J recombinase and telomerase, all remain of unknown function. Here we investigate the activity of the human SETMAR protein, a highly expressed fusion between a histone H3 methylase and a mariner fami...

journal_title：Molecular and cellular biology

authors： Liu D,Bischerour J,Siddique A,Buisine N,Bigot Y,Chalmers R

更新日期：2007-02-01 00:00:00

abstract：:The ErbB family includes four homologous transmembrane tyrosine kinases. Whereas ErbB-1 binds to the epidermal growth factor (EGF), both ErbB-3 and ErbB-4 bind to the Neu differentiation factors (NDFs, or neuregulins), and ErbB-2, the most oncogenic family member, is an orphan receptor whose function is still unknown....

journal_title：Molecular and cellular biology

authors： Tzahar E,Waterman H,Chen X,Levkowitz G,Karunagaran D,Lavi S,Ratzkin BJ,Yarden Y

更新日期：1996-10-01 00:00:00

abstract：:Activation of PERK following the accumulation of unfolded proteins in the endoplasmic reticulum (ER) promotes translation inhibition and cell cycle arrest. PERK function is essential for cell survival following exposure of cells to ER stress, but the mechanisms whereby PERK signaling promotes cell survival are not tho...

journal_title：Molecular and cellular biology

authors： Cullinan SB,Zhang D,Hannink M,Arvisais E,Kaufman RJ,Diehl JA

更新日期：2003-10-01 00:00:00

abstract：:The carcinoembryonic antigen (CEA) family consists of a large group of evolutionarily divergent glycoproteins. The secreted pregnancy-specific glycoproteins constitute a subgroup within the CEA family. They are predominantly expressed in trophoblast cells throughout placental development and are essential for a positi...

journal_title：Molecular and cellular biology

authors： Finkenzeller D,Fischer B,McLaughlin J,Schrewe H,Ledermann B,Zimmermann W

更新日期：2000-10-01 00:00:00

abstract：:Eukaryotic viruses have devised numerous strategies to downregulate activity of the interferon-induced, double-stranded (dsRNA)-activated protein kinase (referred to as p68 on the basis of its Mr of 68,000 in human cells). Viruses must exert this control to avoid extensive phosphorylation of the alpha subunit of eukar...

journal_title：Molecular and cellular biology

authors： Katze MG,Wambach M,Wong ML,Garfinkel M,Meurs E,Chong K,Williams BR,Hovanessian AG,Barber GN

更新日期：1991-11-01 00:00:00

abstract：:The stress-responsive DDR2 gene (previously called DDRA2) of Saccharomyces cerevisiae is transcribed at elevated levels following stress caused by heat shock or DNA damage. Previously, we identified a 51-bp promoter fragment, oligo31/32, which conferred heat shock inducibility on the heterologous CYC1-lacZ reporter ge...

journal_title：Molecular and cellular biology

authors： Kobayashi N,McEntee K

更新日期：1993-01-01 00:00:00

abstract：:GATA-3 is a zinc finger transcription factor which is expressed in a highly restricted and strongly conserved tissue distribution pattern in vertebrate organisms, specifically, in a subset of hematopoietic cells, in cells within the central and peripheral nervous systems, in the kidney, and in placental trophoblasts. ...

journal_title：Molecular and cellular biology

authors： Yang Z,Gu L,Romeo PH,Bories D,Motohashi H,Yamamoto M,Engel JD

更新日期：1994-03-01 00:00:00

abstract：:Fragments of the TAR DNA-binding protein 43 (TDP43) are major components of intracellular aggregates associated with amyotrophic lateral sclerosis and frontotemporal dementia. A variety of C-terminal fragments (CTFs) exist, with distinct N termini; however, little is known regarding their differences in metabolism and...

journal_title：Molecular and cellular biology

authors： Kasu YAT,Alemu S,Lamari A,Loew N,Brower CS

更新日期：2018-09-14 00:00:00

abstract：:SCIP is a POU domain transcription factor expressed by glial progenitor cells in the peripheral and central nervous systems (dividing Schwann cells and O-2A cells, respectively), where it appears to act as a repressor of myelin-specific genes. We have isolated genomic clones encoding the rat SCIP gene. Comparison of t...

journal_title：Molecular and cellular biology

authors： Kuhn R,Monuki ES,Lemke G

更新日期：1991-09-01 00:00:00

abstract：:Bone-specific transcription of the osteocalcin (OC) gene is regulated principally by the Runx2 transcription factor and is further stimulated in response to 1alpha,25-dihydroxyvitamin D3 via its specific receptor (VDR). The rat OC gene promoter contains three recognition sites for Runx2 (sites A, B, and C). Mutation o...

journal_title：Molecular and cellular biology

authors： Paredes R,Arriagada G,Cruzat F,Villagra A,Olate J,Zaidi K,van Wijnen A,Lian JB,Stein GS,Stein JL,Montecino M

更新日期：2004-10-01 00:00:00

abstract：:Generally, histone deacetylase (HDAC) inhibitor-induced p21(Waf1/Cip1) expression is thought to be p53 independent. Here we found that an inhibitor of HDAC, depsipeptide (FR901228), but not trichostatin A (TSA), induces p21(Waf1/Cip1) expression through both p53 and Sp1/Sp3 pathways in A549 cells (which retain wild-ty...

journal_title：Molecular and cellular biology

authors： Zhao Y,Lu S,Wu L,Chai G,Wang H,Chen Y,Sun J,Yu Y,Zhou W,Zheng Q,Wu M,Otterson GA,Zhu WG

更新日期：2006-04-01 00:00:00

abstract：:Cholera toxin (CT) activates expression of two immediate-early response genes (JE and TIS10) in quiescent BALB/c 3T3 cells. Increases in cyclic AMP (cAMP) levels in response to CT are likely responsible for the induction of TIS10 gene expression, since treatment with 8-Br-cAMP and increasing the intracellular levels o...

journal_title：Molecular and cellular biology

authors： Qureshi SA,Alexandropoulos K,Joseph CK,Spangler R,Foster DA

更新日期：1991-01-01 00:00:00

abstract：:Yeast metallothionein, encoded by the CUP1 gene, and its copper-dependent transcriptional activator ACE1 play a key role in mediating copper resistance in Saccharomyces cerevisiae. Using an ethyl methanesulfonate mutant of a yeast strain in which CUP1 and ACE1 were deleted, we isolated a gene, designated CUP9, which p...

journal_title：Molecular and cellular biology

authors： Knight SA,Tamai KT,Kosman DJ,Thiele DJ

更新日期：1994-12-01 00:00:00

abstract：:The transcriptional corepressor mSin3 is associated with histone deacetylases (HDACs) and is utilized by many DNA-binding transcriptional repressors. We have cloned and characterized a novel mSin3A-binding protein, SAP25. SAP25 binds to the PAH1 domain of mSin3A, associates with the mSin3A-HDAC complex in vivo, and re...

journal_title：Molecular and cellular biology

authors： Shiio Y,Rose DW,Aur R,Donohoe S,Aebersold R,Eisenman RN

更新日期：2006-02-01 00:00:00

abstract：:RNA editing in trypanosomes has been proposed to occur through transesterification or endonuclease cleavage and RNA ligation reactions. Both models involve a chimeric intermediate in which a guide RNA (gRNA) is joined through its 3' oligo(U) tail to an editing site of the corresponding mRNA. Velocity centrifugation of...

journal_title：Molecular and cellular biology

authors： Piller KJ,Decker CJ,Rusché LN,Sollner-Webb B

更新日期：1995-06-01 00:00:00

abstract：:Saccharomyces cerevisiae mutant E124 was selected in a visual screen based on elongated cell shape. Genetic analysis showed that E124 contains two separate mutations, pps1-1 and elm4-1, each causing a distinct phenotype inherited as a single-gene trait. In rich medium, pps1-1 by itself causes increased doubling time b...

journal_title：Molecular and cellular biology

authors： Blacketer MJ,Madaule P,Myers AM

更新日期：1994-07-01 00:00:00

abstract：:X chromosome inactivation is a remarkable example of chromosome-wide gene silencing and facultative heterochromatin formation. Numerous histone posttranslational modifications, including H3K9me2 and H3K27me3, accompany this process, although our understanding of the enzymes that lay down these marks and the factors th...

journal_title：Molecular and cellular biology

authors： Escamilla-Del-Arenal M,da Rocha ST,Spruijt CG,Masui O,Renaud O,Smits AH,Margueron R,Vermeulen M,Heard E

更新日期：2013-12-01 00:00:00

abstract：:Circadian clocks keep time via gene expression feedback loops that are controlled by time-of-day-specific changes in the synthesis, activity, and degradation of transcription factors. Within the Drosophila melanogaster circadian clock, DOUBLETIME (DBT) kinase is necessary for the phosphorylation of PERIOD (PER), a tra...

journal_title：Molecular and cellular biology

authors： Yu W,Zheng H,Price JL,Hardin PE

更新日期：2009-03-01 00:00:00

abstract：:The TIP60 histone acetyltransferase plays diverse roles in DNA damage responses, DNA double-strand break repair, and transcriptional regulation. TIP60 resides within a multisubunit complex that has been shown to be targeted by transcription factors and to be involved in histone acetylation and transcriptional activati...

journal_title：Molecular and cellular biology

authors： Park JH,Sun XJ,Roeder RG

更新日期：2010-06-01 00:00:00

abstract：:Gonadotropin-releasing hormone (GnRH) interacts with a G protein-coupled receptor and increases the transcription of the glycoprotein hormone alpha-subunit gene. We have explored the possibility that mitogen-activated protein kinase (MAPK) plays a role in mediating GnRH effects on transcription. Activation of the MAPK...

journal_title：Molecular and cellular biology

authors： Roberson MS,Misra-Press A,Laurance ME,Stork PJ,Maurer RA

更新日期：1995-07-01 00:00:00

abstract：:Previous investigations have defined three upstream activation elements--CCAC, A/T, and TATA sequences--necessary for muscle-specific transcription of the myoglobin gene. In the present study, we demonstrate that these three sequences elements, prepared as synthetic oligonucleotide cassettes, function synergistically ...

journal_title：Molecular and cellular biology

authors： Grayson J,Williams RS,Yu YT,Bassel-Duby R

更新日期：1995-04-01 00:00:00

abstract：:We determined the sequence of a Drosophila tRNA gene cluster containing a tRNAHis gene and a tRNAHis pseudogene in close proximity on the same DNA strand. The pseudogene contains eight consecutive base pairs different from the region of the bona fide gene which codes for the 3' portion of the anticodon stem of tRNAHis...

journal_title：Molecular and cellular biology

authors： Cooley L,Schaack J,Burke DJ,Thomas B,Söll D

更新日期：1984-12-01 00:00:00

abstract：:Activation of multiple signaling pathways is required to trigger the full spectrum of in vitro and in vivo phenotypic traits associated with neoplastic transformation by oncogenic Ras. To determine which of these pathways are important for N-ras tumorigenesis in human cancer cells and also to investigate the possibili...

journal_title：Molecular and cellular biology

authors： Gupta S,Plattner R,Der CJ,Stanbridge EJ

更新日期：2000-12-01 00:00:00

abstract：:A new dominant amplifiable selective system for use in bacterium-animal cell shuttle vectors was developed by the insertion of a 2-kilobase genomic fragment containing the cloned Escherichia coli gene for asparagine synthetase (AS) into the pBR322-simian virus 40 recombinant vector pSV2 so as to place the translationa...

journal_title：Molecular and cellular biology

authors： Cartier M,Chang MW,Stanners CP

更新日期：1987-05-01 00:00:00

abstract：:Downregulation in expression of the c-myc proto-oncogene is an early molecular event in differentiation of murine C2C12 myoblasts into multinucleated myotubes. During differentiation, levels of c-myc mRNA decrease 3- to 10-fold despite a lack of change in its transcription rate. To identify cis-acting elements that ta...

journal_title：Molecular and cellular biology

authors： Yeilding NM,Lee WM

更新日期：1997-05-01 00:00:00

abstract：:Transcription factor CREB regulates cyclic AMP (cAMP)-dependent gene expression by binding to and activating transcription from cAMP response elements (CREs) in the promoters of target genes. The transcriptional transactivation functions of CREB are activated by its phosphorylation by cAMP-dependent protein kinase A (...

journal_title：Molecular and cellular biology

authors： Vallejo M,Gosse ME,Beckman W,Habener JF

更新日期：1995-01-01 00:00:00