The Saccharomyces cerevisiae mutation elm4-1 facilitates pseudohyphal differentiation and interacts with a deficiency in phosphoribosylpyrophosphate synthase activity to cause constitutive pseudohyphal growth.


:Saccharomyces cerevisiae mutant E124 was selected in a visual screen based on elongated cell shape. Genetic analysis showed that E124 contains two separate mutations, pps1-1 and elm4-1, each causing a distinct phenotype inherited as a single-gene trait. In rich medium, pps1-1 by itself causes increased doubling time but does not affect cell shape, whereas elm4-1 results in a moderate cell elongation phenotype but does not affect growth rate. Reconstructed elm4-1 pps1-1 double mutants display a synthetic phenotype in rich medium including extreme cell elongation and delayed cell separation, both characteristics of pseudohyphal differentiation. The elm4-1 mutation was shown to act as a dominant factor that potentiates pseudohyphal differentiation in response to general nitrogen starvation in a genetic background in which pseudohyphal growth normally does not occur. Thus, elm4-1 allows recognition of, or response to, a pseudohyphal differentiation signal that results from nitrogen limitation. PPS1 was isolated and shown to be a previously undescribed gene coding for a protein similar in amino acid sequence to phosphoribosylpyrophosphate synthase, a rate-limiting enzyme in the biosynthesis of nucleotides, histidine, and tryptophan. Thus, the pps1-1 mutation may generate a nitrogen limitation signal, which when coupled with elm4-1 results in pseudohyphal growth even in rich medium.


Mol Cell Biol


Blacketer MJ,Madaule P,Myers AM




Has Abstract


1994-07-01 00:00:00












  • Preparation of a "functional library" of African green monkey DNA fragments which substitute for the processing/polyadenylation signal in the herpes simplex virus type 1 thymidine kinase gene.

    abstract::Fragments of African green monkey (Cercopithecus aethiops) DNA (3.5 to 18.0 kilobases) were inserted downstream from the thymidine kinase (TK, tk) coding region in pTK206/SV010, a gene construct which lacks both copies of the hexanucleotide 5'-AATAAA-3' and contains a simian virus 40 origin of replication, allowing it...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Santangelo GM,Cole CN

    更新日期:1983-04-01 00:00:00

  • Mutations affecting the structure and function of immunoglobulin M.

    abstract::Using a hybridoma cell line which secretes hapten-specific immunoglobulin M (IgM), we have isolated a variety of mutants which produce abnormal immunoglobulin. Immunoglobulin was tested for the size and composition of the component heavy and light chains and for variable and constant region related functional and sero...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Shulman MJ,Heusser C,Filkin C,Köhler G

    更新日期:1982-09-01 00:00:00

  • CRM1 mediates the export of ADAR1 through a nuclear export signal within the Z-DNA binding domain.

    abstract::RNA editing of specific residues by adenosine deamination is a nuclear process catalyzed by adenosine deaminases acting on RNA (ADAR). Different promoters in the ADAR1 gene give rise to two forms of the protein: a constitutive promoter expresses a transcript encoding (c)ADAR1, and an interferon-induced promoter expres...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Poulsen H,Nilsson J,Damgaard CK,Egebjerg J,Kjems J

    更新日期:2001-11-01 00:00:00

  • Requirement of PDZ-containing proteins for cell cycle regulation and differentiation in the mouse lens epithelium.

    abstract::The roles of PDZ domain-containing proteins such as Dlg and Scrib have been well described for Drosophila; however, their requirement for mammalian development is poorly understood. Here we show that Dlg, Scrib, MAGI1, MAGI3, and MPDZ are expressed in the mouse ocular lens. We demonstrate that the increase in prolifer...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Nguyen MM,Nguyen ML,Caruana G,Bernstein A,Lambert PF,Griep AE

    更新日期:2003-12-01 00:00:00

  • The petD gene is transcribed by functionally redundant promoters in Chlamydomonas reinhardtii chloroplasts.

    abstract::FUD6, a nonphotosynthetic mutant of Chlamydomonas reinhardtii, was previously found to be deficient in the synthesis of subunit IV of the cytochrome b6/f complex, the chloroplast petD gene product (C. Lemaire, J. Girard-Bascou, F.-A. Wollman, and P. Bennoun, Biochim. Biophys. Acta 851:229-238, 1986). The lesion in FUD...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Sturm NR,Kuras R,Büschlen S,Sakamoto W,Kindle KL,Stern DB,Wollman FA

    更新日期:1994-09-01 00:00:00

  • Molecular analysis of the distal enhancer of the mouse alpha-fetoprotein gene.

    abstract::The mouse alpha-fetoprotein (AFP) gene is transcribed at high levels in the visceral endoderm of the yolk sac and fetal liver and at much lower rates in the endoderm of the fetal gut. Expression of the gene in vivo requires the presence of at least one of three enhancers which lie in its 5' flanking region. In this re...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Millonig JH,Emerson JA,Levorse JM,Tilghman SM

    更新日期:1995-07-01 00:00:00

  • The transcription factors c-rel and RelA control epidermal development and homeostasis in embryonic and adult skin via distinct mechanisms.

    abstract::Determining the roles of Rel/NF-kappaB transcription factors in mouse skin development with loss-of-function mutants has been limited by redundancy among these proteins and by embryonic lethality associated with the absence of RelA. Using mice lacking RelA and c-rel, which survive throughout embryogenesis on a tumor n...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Gugasyan R,Voss A,Varigos G,Thomas T,Grumont RJ,Kaur P,Grigoriadis G,Gerondakis S

    更新日期:2004-07-01 00:00:00

  • Phosphorylation of p21 in G2/M promotes cyclin B-Cdc2 kinase activity.

    abstract::Little is known about the posttranslational control of the cyclin-dependent protein kinase (CDK) inhibitor p21. We describe here a transient phosphorylation of p21 in the G2/M phase. G2/M-phosphorylated p21 is short-lived relative to hypophosphorylated p21. p21 becomes nuclear during S phase, prior to its phosphorylat...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Dash BC,El-Deiry WS

    更新日期:2005-04-01 00:00:00

  • Development of autonomously replicating plasmids for Candida albicans.

    abstract::A pool of Candida albicans RsaI fragments cloned onto a vector containing pBR322 sequences and the Candida ADE2 gene was used to transform a Candida ade2 mutant to adenine protrophy. A potential autonomously replicating sequence (ARS) in Candida DNA was identified by two criteria: instability of the selectable marker ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Kurtz MB,Cortelyou MW,Miller SM,Lai M,Kirsch DR

    更新日期:1987-01-01 00:00:00

  • The rapamycin-binding domain governs substrate selectivity by the mammalian target of rapamycin.

    abstract::The mammalian target of rapamycin (mTOR) is a Ser/Thr (S/T) protein kinase, which controls mRNA translation initiation by modulating phosphorylation of the translational regulators PHAS-I and p70(S6K). Here we show that in vitro mTOR is able to phosphorylate these two regulators at comparable rates. Both (S/T)P sites,...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: McMahon LP,Choi KM,Lin TA,Abraham RT,Lawrence JC Jr

    更新日期:2002-11-01 00:00:00

  • Abortive gap repair: underlying mechanism for Ds element formation.

    abstract::The mechanism by which the maize autonomous Ac transposable element gives rise to nonautonomous Ds elements is largely unknown. Sequence analysis of native maize Ds elements indicates a complex chimeric structure formed through deletions of Ac sequences with or without insertions of Ac-unrelated sequence blocks. These...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Rubin E,Levy AA

    更新日期:1997-11-01 00:00:00

  • Quantitative analysis of gene suppression in integrated retrovirus vectors.

    abstract::Previously, we described a retrovirus vector that contained two genes: a 5' gene under transcriptional control of the viral long terminal repeat and a 3' gene under transcriptional control of the herpes simplex virus thymidine kinase promoter. By using a biological assay, we found that expression of the 5' gene is sup...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Emerman M,Temin HM

    更新日期:1986-03-01 00:00:00

  • Isoform-specific complementation of the yeast sac6 null mutation by human fimbrin.

    abstract::The actin cytoskeleton is a fundamental component of eukaryotic cells, with both structural and motile roles. Actin and many of the actin-binding proteins found in different cell types are highly conserved, showing considerable similarity in both primary structure and biochemical properties. To make detailed compariso...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Adams AE,Shen W,Lin CS,Leavitt J,Matsudaira P

    更新日期:1995-01-01 00:00:00

  • Sterol regulatory element binding protein 1a regulates hepatic fatty acid partitioning by activating acetyl coenzyme A carboxylase 2.

    abstract::We generated a line of mice in which sterol regulatory element binding protein 1a (SREBP-1a) was specifically inactivated by insertional mutagenesis. Homozygous mutant mice were completely viable despite expressing SREBP-1a mRNA below 5% of normal, and there were minimal effects on expression of either SREBP-1c or -2....

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Im SS,Hammond LE,Yousef L,Nugas-Selby C,Shin DJ,Seo YK,Fong LG,Young SG,Osborne TF

    更新日期:2009-09-01 00:00:00

  • Two novel protein-tyrosine kinases, each with a second phosphotransferase-related catalytic domain, define a new class of protein kinase.

    abstract::The protein-tyrosine kinases (PTKs) are a burgeoning family of proteins, each of which bears a conserved domain of 250 to 300 amino acids capable of phosphorylating substrate proteins on tyrosine residues. We recently exploited the existence of two highly conserved sequence elements within the catalytic domain to gene...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Wilks AF,Harpur AG,Kurban RR,Ralph SJ,Zürcher G,Ziemiecki A

    更新日期:1991-04-01 00:00:00

  • Rev7/MAD2B links c-Jun N-terminal protein kinase pathway signaling to activation of the transcription factor Elk-1.

    abstract::The mitogen-activated protein (MAP) kinases represent one of the most important classes of signaling cascades that are used by eukaryotic cells to sense extracellular signals. One of the major responses to these cascades is a change in cellular gene expression profiles mediated through the direct targeting of transcri...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Zhang L,Yang SH,Sharrocks AD

    更新日期:2007-04-01 00:00:00

  • RotundRacGAP functions with Ras during spermatogenesis and retinal differentiation in Drosophila melanogaster.

    abstract::Our analysis of rotund (rn) null mutations in Drosophila melanogaster revealed that deletion of the rn locus affects both spermatid and retinal differentiation. In the male reproductive system, the absence of RnRacGAP induced small testes, empty seminal vesicles, short testicular cysts, reduced amounts of interspermat...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Bergeret E,Pignot-Paintrand I,Guichard A,Raymond K,Fauvarque MO,Cazemajor M,Griffin-Shea R

    更新日期:2001-09-01 00:00:00

  • Effects of rho kinase and actin stress fibers on sustained extracellular signal-regulated kinase activity and activation of G(1) phase cyclin-dependent kinases.

    abstract::We recently reported that Rho kinase is required for sustained ERK signaling and the consequent mid-G(1) phase induction of cyclin D1 in fibroblasts. The results presented here indicate that these Rho kinase effects are mediated by the formation of stress fibers and the consequent clustering of alpha5beta1 integrin. M...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章,收录出版


    authors: Roovers K,Assoian RK

    更新日期:2003-06-01 00:00:00

  • Insulin stimulates syntaxin4 SNARE complex assembly via a novel regulatory mechanism.

    abstract::Insulin stimulates glucose transport into fat and muscle cells by increasing the exocytic trafficking rate of the GLUT4 facilitative glucose transporter from intracellular stores to the plasma membrane. Delivery of GLUT4 to the plasma membrane is mediated by formation of functional SNARE complexes containing syntaxin4...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Kioumourtzoglou D,Gould GW,Bryant NJ

    更新日期:2014-04-01 00:00:00

  • Serum response factor and protein-mediated DNA bending contribute to transcription of the dystrophin muscle-specific promoter.

    abstract::The minimal muscle-specific dystrophin promoter contains the consensus sequence CC(A/T)6GG, or the CArG element, which can be found in serum-inducible or muscle-specific promoters. The serum response factor (SRF), which mediates the transcriptional activation of the c-fos gene in response to serum stimulation, can bin...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Galvagni F,Lestingi M,Cartocci E,Oliviero S

    更新日期:1997-03-01 00:00:00

  • Transcriptional induction by aromatic amino acids in Saccharomyces cerevisiae.

    abstract::Aromatic aminotransferase II, product of the ARO9 gene, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism in Saccharomyces cerevisiae. ARO9 expression is under the dual control of specific induction and nitrogen source regulation. We have here identified UASaro, a 36-bp upstream element ne...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Iraqui I,Vissers S,André B,Urrestarazu A

    更新日期:1999-05-01 00:00:00

  • Cdc37 promotes the stability of protein kinases Cdc28 and Cak1.

    abstract::In the budding yeast Saccharomyces cerevisiae, Cdc37 is required for the productive formation of Cdc28-cyclin complexes. The cdc37-1 mutant arrests at Start with low levels of Cdc28 protein, which is predominantly unphosphorylated at Thr169, fails to bind cyclin, and has little protein kinase activity. We show here th...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Farrell A,Morgan DO

    更新日期:2000-02-01 00:00:00

  • Occludin S471 Phosphorylation Contributes to Epithelial Monolayer Maturation.

    abstract::Multiple organ systems require epithelial barriers for normal function, and barrier loss is a hallmark of diseases ranging from inflammation to epithelial cancers. However, the molecular processes regulating epithelial barrier maturation are not fully elucidated. After contact, epithelial cells undergo size-reductive ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Bolinger MT,Ramshekar A,Waldschmidt HV,Larsen SD,Bewley MC,Flanagan JM,Antonetti DA

    更新日期:2016-07-14 00:00:00

  • Disruption of the Rb--Raf-1 interaction inhibits tumor growth and angiogenesis.

    abstract::The retinoblastoma tumor suppressor protein (Rb) plays a vital role in regulating mammalian cell cycle progression and inactivation of Rb is necessary for entry into S phase. Rb is inactivated by phosphorylation upon growth factor stimulation of quiescent cells, facilitating the transition from G(1) phase to S phase. ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Dasgupta P,Sun J,Wang S,Fusaro G,Betts V,Padmanabhan J,Sebti SM,Chellappan SP

    更新日期:2004-11-01 00:00:00

  • Characterization of the dead ringer gene identifies a novel, highly conserved family of sequence-specific DNA-binding proteins.

    abstract::We reported the identification of a new family of DNA-binding proteins from our characterization of the dead ringer (dri) gene of Drosophila melanogaster. We show that dri encodes a nuclear protein that contains a sequence-specific DNA-binding domain that bears no similarity to known DNA-binding domains. A number of p...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Gregory SL,Kortschak RD,Kalionis B,Saint R

    更新日期:1996-03-01 00:00:00

  • Densely methylated DNA islands in mammalian chromosomal replication origins.

    abstract::Densely methylated DNA sequence islands, designated DMIs, have been observed in two Chinese hamster cell chromosomal replication origins by using a PCR-based chemical method of detection. One of the origins, oriS14, is located within or adjacent to the coding sequence for ribosomal protein S14 on chromosome 2q, and th...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Tasheva ES,Roufa DJ

    更新日期:1994-09-01 00:00:00

  • Characterization of a family of related cellular transcription factors which can modulate human immunodeficiency virus type 1 transcription in vitro.

    abstract::LBP-1 is a cellular protein which binds strongly to sequences around the human immunodeficiency virus type 1 (HIV-1) initiation site and weakly over the TATA box. We have previously shown that LBP-1 represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA box. Four similar but distinct cDNAs encodi...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Yoon JB,Li G,Roeder RG

    更新日期:1994-03-01 00:00:00

  • Type 1 transforming growth factor beta: amplified expression and secretion of mature and precursor polypeptides in Chinese hamster ovary cells.

    abstract::Recombinant type 1 transforming growth factor beta (TGF-beta) was expressed to high levels in CHO cells by using dihydrofolate reductase (dhfr) gene amplification. The expression plasmid was derived from the pSV2 vectors and contained, in tandem, the simian TGF-beta and mouse dhfr cDNAs. Transcription of both cDNAs wa...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Gentry LE,Webb NR,Lim GJ,Brunner AM,Ranchalis JE,Twardzik DR,Lioubin MN,Marquardt H,Purchio AF

    更新日期:1987-10-01 00:00:00

  • Insulin-stimulated disassociation of the SOS-Grb2 complex.

    abstract::Insulin stimulation of differentiated 3T3-L1 adipocytes or Chinese hamster ovary cells expressing high levels of the insulin receptor resulted in a time-dependent decrease in the electrophoretic mobility of SOS on sodium dodecyl sulfate-polyacrylamide gels. The reduction in SOS mobility was completely reversed by alka...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Waters SB,Yamauchi K,Pessin JE

    更新日期:1995-05-01 00:00:00

  • Evidence for involvement of activin A and bone morphogenetic protein 4 in mammalian mesoderm and hematopoietic development.

    abstract::Xenopus in vitro studies have implicated both transforming growth factor beta (TGF-beta) and fibroblast growth factor (FGF) families in mesoderm induction. Although members of both families are present during mouse mesoderm formation, there is little evidence for their functional role in mesoderm induction. We show th...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Johansson BM,Wiles MV

    更新日期:1995-01-01 00:00:00