Abstract:
:The multisubunit yeast transcription factor IIIC (TFIIIC) is a multifunctional protein required for promoter recognition, transcription factor IIIB recruitment, and chromatin antirepression. We report the isolation and characterization of TFC7, an essential gene encoding the 55-kDa polypeptide, tau55, present in affinity-purified TFIIIC. tau55 is a chimeric protein generated by an ancient chromosomal rearrangement. Its C-terminal half is essential for cell viability and sufficient to ensure TFIIIC function in DNA binding and transcription assays. The N-terminal half is nonessential and highly similar to a putative yeast protein encoded on another chromosome and to a cyanobacterial protein of unknown function. Partial deletions of the N-terminal domain impaired tau55 function at a high temperature or in media containing glycerol or ethanol, suggesting a link between PolIII transcription and metabolic pathways. Interestingly, tau55 was found, together with TFIIIC subunit tau95, in a protein complex which was distinct from TFIIIC and which may play a role in the regulation of PolIII transcription, possibly in relation to cell metabolism.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Manaud N,Arrebola R,Buffin-Meyer B,Lefebvre O,Voss H,Riva M,Conesa C,Sentenac Adoi
10.1128/mcb.18.6.3191subject
Has Abstractpub_date
1998-06-01 00:00:00pages
3191-200issue
6eissn
0270-7306issn
1098-5549journal_volume
18pub_type
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