Abstract:
:SCIP is a POU domain transcription factor expressed by glial progenitor cells in the peripheral and central nervous systems (dividing Schwann cells and O-2A cells, respectively), where it appears to act as a repressor of myelin-specific genes. We have isolated genomic clones encoding the rat SCIP gene. Comparison of the structure of these clones with genomic Southern blots and SCIP cDNAs demonstrates that SCIP is encoded in a single-copy, intronless gene that has the general features of an expressed retroposon. This gene contributes to an extended CpG island. It is transcribed to produce a 3.1-kb mRNA that encodes a 451-amino-acid protein with a predicted molecular mass of 45 kDa. Immunopurified SCIP antibodies specifically recognize a nuclear protein of this size in cultured proliferating Schwann cells, and gel shift analyses demonstrate that this protein is the predominant octamer-binding protein in these cells.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Kuhn R,Monuki ES,Lemke Gdoi
10.1128/mcb.11.9.4642subject
Has Abstractpub_date
1991-09-01 00:00:00pages
4642-50issue
9eissn
0270-7306issn
1098-5549journal_volume
11pub_type
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