Abstract:
:Mutations in the Drosophila mus308 gene confer specific hypersensitivity to DNA-cross-linking agents as a consequence of defects in DNA repair. The mus308 gene is shown here to encode a 229-kDa protein in which the amino-terminal domain contains the seven conserved motifs characteristic of DNA and RNA helicases and the carboxy-terminal domain shares over 55% sequence similarity with the polymerase domains of prokaryotic DNA polymerase I-like enzymes. This is the first reported member of this family of DNA polymerases in a eukaryotic organism, as well as the first example of a single polypeptide with homology to both DNA polymerase and helicase motifs. Identification of a closely related gene in the genome of Caenorhabditis elegans suggests that this novel polypeptide may play an evolutionarily conserved role in the repair of DNA damage in eukaryotic organisms.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Harris PV,Mazina OM,Leonhardt EA,Case RB,Boyd JB,Burtis KCdoi
10.1128/mcb.16.10.5764subject
Has Abstractpub_date
1996-10-01 00:00:00pages
5764-71issue
10eissn
0270-7306issn
1098-5549journal_volume
16pub_type
杂志文章abstract::Fusions between the TRM1 gene of Saccharomyces cerevisiae and COXIV or DHFR were made to examine the mitochondrial targeting signals of N2,N2-dimethylguanosine-specific tRNA methyltransferase [tRNA (m2(2)G)dimethyltransferase]. This enzyme is responsible for the modification of both mitochondrial and cytoplasmic tRNAs...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1990-09-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2003-05-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:2016-10-28 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2006-03-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.01283-06
更新日期:2007-01-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.26.10.3853-3863.2006
更新日期:2006-05-01 00:00:00
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doi:10.1128/mcb.18.6.3596
更新日期:1998-06-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1984-03-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:1991-10-01 00:00:00
abstract::Recent advancements in genetic and proteomic technologies have revealed that more of the genome encodes proteins than originally thought possible. Specifically, some putative long noncoding RNAs (lncRNAs) have been misannotated as noncoding. Numerous lncRNAs have been found to contain short open reading frames (sORFs)...
journal_title:Molecular and cellular biology
pub_type: 杂志文章,评审
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更新日期:2020-02-27 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:2013-04-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.17.3.1522
更新日期:1997-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-01-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
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更新日期:1996-08-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/mcb.13.6.3176
更新日期:1993-06-01 00:00:00
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journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.21.24.8336-8345.2001
更新日期:2001-12-01 00:00:00
abstract::To distinguish among possible mechanisms of repair of a double-strand break (DSB) by gene conversion in budding yeast, Saccharomyces cerevisiae, we employed isotope density transfer to analyze budding yeast mating type (MAT) gene switching in G2/M-arrested cells. Both of the newly synthesized DNA strands created durin...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
doi:10.1128/MCB.01654-06
更新日期:2006-12-01 00:00:00