When Long Noncoding Becomes Protein Coding.


:Recent advancements in genetic and proteomic technologies have revealed that more of the genome encodes proteins than originally thought possible. Specifically, some putative long noncoding RNAs (lncRNAs) have been misannotated as noncoding. Numerous lncRNAs have been found to contain short open reading frames (sORFs) which have been overlooked because of their small size. Many of these sORFs encode small proteins or micropeptides with fundamental biological importance. These micropeptides can aid in diverse processes, including cell division, transcription regulation, and cell signaling. Here we discuss strategies for establishing the coding potential of putative lncRNAs and describe various functions of known micropeptides.


Mol Cell Biol


Hartford CCR,Lal A




Has Abstract


2020-02-27 00:00:00












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    authors: Deng X,Gao F,Flagg T,Anderson J,May WS

    更新日期:2006-06-01 00:00:00

  • Structure and expression of STK, a src-related gene in the simple metazoan Hydra attenuata.

    abstract::Both cDNA clones and a genomic DNA clone encoding a 509-amino-acid protein that is 64% similar to chicken pp60c-src were isolated from the simple metazoan Hydra attenuata. We have designated this gene STK, for src-type kinase. Features of the amino acid sequence of the protein encoded by the STK gene suggest that it i...

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    authors: Bosch TC,Unger TF,Fisher DA,Steele RE

    更新日期:1989-10-01 00:00:00

  • Binding and uptake of diphtheria toxin by toxin-resistant Chinese hamster ovary and mouse cells.

    abstract::We investigated two phenotypically distinct types of diphtheria toxin-resistant mutants of Chinese hamster cells and compared their resistance with that of naturally resistant mouse cells. All are resistant due to a defect in the process of internalization and delivery of toxin to its target in the cytosol, elongation...

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    authors: Didsbury JR,Moehring JM,Moehring TJ

    更新日期:1983-07-01 00:00:00

  • Biochemical and functional analyses of a secreted H-2Ld molecule.

    abstract::A truncated H-2Ld gene was constructed by deleting the transmembrane and cytoplasmic exons. The truncated H-2Ld gene was introduced into mouse L cells using the thymidine kinase gene as a selectable marker. Transformants were isolated and screened for the presence of truncated H-2Ld antigen. The truncated H-2Ld gene p...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Murre C,Parker KC,Reiss CS,Driller JP,Wiley DC,Burakoff SJ,Seidman JG

    更新日期:1986-04-01 00:00:00

  • Densely methylated DNA islands in mammalian chromosomal replication origins.

    abstract::Densely methylated DNA sequence islands, designated DMIs, have been observed in two Chinese hamster cell chromosomal replication origins by using a PCR-based chemical method of detection. One of the origins, oriS14, is located within or adjacent to the coding sequence for ribosomal protein S14 on chromosome 2q, and th...

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    authors: Tasheva ES,Roufa DJ

    更新日期:1994-09-01 00:00:00

  • Saccharomyces cerevisiae killer virus transcripts contain template-coded polyadenylate tracts.

    abstract::The M double-stranded RNA component of type 1 killer strains of the yeast Saccharomyces cerevisiae contains an internal 200-base pair adenine- and uracil-rich region. The plus strands of this viral genomic RNA contain an internal adenine-rich region which allows these strands to bind to polyuridylate-Sepharose as tigh...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Hannig EM,Thiele DJ,Leibowitz MJ

    更新日期:1984-01-01 00:00:00

  • Disruption of transforming growth factor beta signaling by a mutation that prevents transphosphorylation within the receptor complex.

    abstract::T beta R-II (transforming growth factor beta [TGF-beta] type II receptor) is a transmembrane serine/threonine kinase that acts as the primary TGF-beta receptor. Ligand binding to T beta R-II leads to the recruitment and phosphorylation of T beta R-I, a distantly related transmembrane kinase that acts as a downstream s...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Cárcamo J,Zentella A,Massagué J

    更新日期:1995-03-01 00:00:00

  • TATA-box DNA binding activity and subunit composition for RNA polymerase III transcription factor IIIB from Xenopus laevis.

    abstract::The RNA polymerase III transcription initiation factor TFIIIB contains the TATA-box-binding protein (TBP) and polymerase III-specific TBP-associated factors (TAFs). Previous studies have shown that DNA oligonucleotides containing the consensus TATA-box sequence inhibit polymerase III transcription, implying that the D...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: McBryant SJ,Meier E,Leresche A,Sharp SJ,Wolf VJ,Gottesfeld JM

    更新日期:1996-09-01 00:00:00

  • Multiple homing pathways used by yeast mitochondrial group II introns.

    abstract::The yeast mitochondrial DNA group II introns aI1 and aI2 are retroelements that insert site specifically into intronless alleles by a process called homing. Here, we used patterns of flanking marker coconversion in crosses with wild-type and mutant aI2 introns to distinguish three coexisting homing pathways: two that ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Eskes R,Liu L,Ma H,Chao MY,Dickson L,Lambowitz AM,Perlman PS

    更新日期:2000-11-01 00:00:00

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    abstract::Activation of the Evi-1 zinc finger gene is a common event associated with transformation of murine myeloid leukemias. To characterize the gene product, we developed antisera against various protein domains. These antisera primarily detected a 145-kilodalton nuclear protein that bound double-stranded DNA. Binding was ...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Matsugi T,Morishita K,Ihle JN

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  • Translation and membrane insertion of the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus.

    abstract::The hemagglutinin-neuraminidase (HN) protein of paramyxoviruses is likely in the unusual class of glycoproteins with the amino terminus cytoplasmic and the carboxy terminus lumenal or external to the cell. The properties of the membrane insertion of the HN protein of Newcastle disease virus, a prototype paramyxovirus,...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Wilson C,Gilmore R,Morrison T

    更新日期:1987-04-01 00:00:00

  • Differential activation of the Ras/extracellular-signal-regulated protein kinase pathway is responsible for the biological consequences induced by the Axl receptor tyrosine kinase.

    abstract::To understand the mechanism of Axl signaling, we have initiated studies to delineate downstream components in interleukin-3-dependent 32D cells by using a chimeric receptor containing the recombinant epidermal growth factor (EGF) receptor extracellular and transmembrane domains and the Axl kinase domain (EAK [for EGF ...

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    authors: Fridell YW,Jin Y,Quilliam LA,Burchert A,McCloskey P,Spizz G,Varnum B,Der C,Liu ET

    更新日期:1996-01-01 00:00:00

  • Fine mapping of 28S rRNA sites specifically cleaved in cells undergoing apoptosis.

    abstract::Bona fide apoptosis in rat and human leukemia cells, rat thymocytes, and bovine endothelial cells was accompanied by limited and specific cleavage of polysome-associated and monosome-associated 28S rRNA, with 18S rRNA being spared. Specific 28S rRNA cleavage was observed in all instances of apoptotic death accompanied...

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    authors: Houge G,Robaye B,Eikhom TS,Golstein J,Mellgren G,Gjertsen BT,Lanotte M,Døskeland SO

    更新日期:1995-04-01 00:00:00

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    authors: Hoi CS,Lee SE,Lu SY,McDermitt DJ,Osorio KM,Piskun CM,Peters RM,Paus R,Tumbar T

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    authors: Judelson HS,Vogt VM

    更新日期:1982-03-01 00:00:00

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    journal_title:Molecular and cellular biology

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    authors: Taghian DG,Nickoloff JA

    更新日期:1997-11-01 00:00:00

  • Transforming ras proteins accelerate hormone-induced maturation and stimulate cyclic AMP phosphodiesterase in Xenopus oocytes.

    abstract::Transforming Harvey (Ha) ras oncogene products accelerated the time course of Xenopus oocyte maturation induced by insulin, insulinlike growth factor 1, or progesterone. The transforming constructs, [Val-12]Ha p21 and [Val-12, Thr-59]Ha p21, displayed equal potency and efficacy in their abilities to accelerate the gro...

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    authors: Sadler SE,Maller JL,Gibbs JB

    更新日期:1990-04-01 00:00:00

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    abstract::In eukaryotic cells, calcium entry across the cell surface activates nuclear gene expression, a process critically important for cell growth and differentiation, learning, and memory and immune cell functions. In immune cells, calcium entry occurs through store-operated Ca(2+) release-activated Ca(2+) (CRAC) channels,...

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    authors: Yeh YC,Parekh AB

    更新日期:2015-04-01 00:00:00

  • Cellular role of yeast Apn1 apurinic endonuclease/3'-diesterase: repair of oxidative and alkylation DNA damage and control of spontaneous mutation.

    abstract::The APN1 gene of Saccharomyces cerevisiae encodes the major apurinic/apyrimidinic endonuclease and 3'-repair DNA diesterase in yeast cell extracts. The Apn1 protein is a homolog of Escherichia coli endonuclease IV, which functions in the repair of some oxidative and alkylation damages in that organism. We show here th...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Ramotar D,Popoff SC,Gralla EB,Demple B

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  • Sterol regulatory element binding protein 1a regulates hepatic fatty acid partitioning by activating acetyl coenzyme A carboxylase 2.

    abstract::We generated a line of mice in which sterol regulatory element binding protein 1a (SREBP-1a) was specifically inactivated by insertional mutagenesis. Homozygous mutant mice were completely viable despite expressing SREBP-1a mRNA below 5% of normal, and there were minimal effects on expression of either SREBP-1c or -2....

    journal_title:Molecular and cellular biology

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    authors: Im SS,Hammond LE,Yousef L,Nugas-Selby C,Shin DJ,Seo YK,Fong LG,Young SG,Osborne TF

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  • Synthetic enhancement of a TFIIB defect by a mutation in SSU72, an essential yeast gene encoding a novel protein that affects transcription start site selection in vivo.

    abstract::An ssu72 mutant of Saccharomyces cerevisiae was identified as an enhancer of a TFIIB defect (sua7-1) that confers both a cold-sensitive growth defect and a downstream shift in transcription start site selection. The ssu72-1 allele did not affect cold sensitivity but, in combination with sua7-1, created a heat-sensitiv...

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    pub_type: 杂志文章


    authors: Sun ZW,Hampsey M

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    journal_title:Molecular and cellular biology

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    authors: Zhang S,Cagatay T,Amanai M,Zhang M,Kline J,Castrillon DH,Ashfaq R,Oz OK,Wharton KA Jr

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  • The centrosomal, putative tumor suppressor protein TACC2 is dispensable for normal development, and deficiency does not lead to cancer.

    abstract::TACC2 is a member of the transforming acidic coiled-coil-containing protein family and is associated with the centrosome-spindle apparatus during cell cycling. In vivo, the TACC2 gene is expressed in various splice forms predominantly in postmitotic tissues, including heart, muscle, kidney, and brain. Studies of human...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Schuendeln MM,Piekorz RP,Wichmann C,Lee Y,McKinnon PJ,Boyd K,Takahashi Y,Ihle JN

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  • Expression of chimeric tRNA-driven antisense transcripts renders NIH 3T3 cells highly resistant to Moloney murine leukemia virus replication.

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Sullenger BA,Lee TC,Smith CA,Ungers GE,Gilboa E

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  • Spatially restricted translation of the xCR1 mRNA in Xenopus embryos.

    abstract::The xCR1 protein is a maternal determinant and cofactor for nodal signaling in vertebrate embryos. The xCR1 protein accumulates specifically in the animal cells of Xenopus embryos, but maternal xCR1 mRNA is distributed equally throughout all embryonic cells. Here, we show that vegetal cell-specific translational repre...

    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Zhang Y,Forinash KD,McGivern J,Fritz B,Dorey K,Sheets MD

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: McLeod M,Craft S,Broach JR

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Johnstone RW,See RH,Sells SF,Wang J,Muthukkumar S,Englert C,Haber DA,Licht JD,Sugrue SP,Roberts T,Rangnekar VM,Shi Y

    更新日期:1996-12-01 00:00:00

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Sun Y,Yuan J,Liu H,Shi Z,Baker K,Vuori K,Wu J,Feng GS

    更新日期:2004-02-01 00:00:00

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Langer SJ,Bortner DM,Roussel MF,Sherr CJ,Ostrowski MC

    更新日期:1992-12-01 00:00:00

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    journal_title:Molecular and cellular biology

    pub_type: 杂志文章


    authors: Hu MC,Sharp SB,Davidson N

    更新日期:1986-01-01 00:00:00