Probing keratinocyte and differentiation specificity of the human K5 promoter in vitro and in transgenic mice.

abstract：:We analyzed the replication of two unlinked actin genes, ardB and ardC , which are abundantly transcribed in the naturally synchronous plasmodium of the slime mold Physarum polycephalum. Detection and size measurements of single-stranded nascent replication intermediates (RIs) demonstrate that these two genes are conc...

journal_title：Molecular and cellular biology

authors： Bénard M,Lagnel C,Pallotta D,Pierron G

更新日期：1996-03-01 00:00:00

abstract：:Both systemic insulin resistance and tissue-specific insulin resistance have been described in Drosophila and are accompanied by many indicators of metabolic disease. The downstream mediators of insulin-resistant pathophysiology remain unclear. We analyzed insulin signaling in the fat body studying loss and gain of fu...

journal_title：Molecular and cellular biology

authors： Musselman LP,Fink JL,Grant AR,Gatto JA,Tuthill BF 2nd,Baranski TJ

更新日期：2017-12-29 00:00:00

abstract：:Myocyte enhancer factor 2 (MEF2) transcription factors cooperate with the MyoD family of basic helix-loop-helix (bHLH) transcription factors to drive skeletal muscle development during embryogenesis, but little is known about the potential functions of MEF2 factors in postnatal skeletal muscle. Here we show that skele...

journal_title：Molecular and cellular biology

authors： Potthoff MJ,Arnold MA,McAnally J,Richardson JA,Bassel-Duby R,Olson EN

更新日期：2007-12-01 00:00:00

abstract：:A key antioxidant pathway, the Keap1-Nrf2 system, is regulated by p62/Sqstm1 via multiple mechanisms, including gene expression, posttranslational modifications (such as ubiquitination and phosphorylation), and autophagic degradation of p62/Sqstm1 and Keap1. Here we demonstrate a novel mode of regulation of the Keap1-...

journal_title：Molecular and cellular biology

authors： Kageyama S,Saito T,Obata M,Koide RH,Ichimura Y,Komatsu M

更新日期：2018-03-15 00:00:00

abstract：:The accurate segregation of chromosomes requires the kinetochore, a complex protein machine that assembles onto centromeric DNA to mediate attachment of replicated sister chromatids to the mitotic spindle apparatus. This study reveals an important role for the yeast RSC ATP-dependent chromatin-remodeling complex at th...

journal_title：Molecular and cellular biology

authors： Hsu JM,Huang J,Meluh PB,Laurent BC

更新日期：2003-05-01 00:00:00

abstract：:The genes of the trithorax group (trxG) in Drosophila melanogaster are required to maintain the pattern of homeotic gene expression that is established early in embryogenesis by the transient expression of the segmentation genes. The precise role of each of the diverse trxG members and the functional relationships amo...

journal_title：Molecular and cellular biology

authors： Crosby MA,Miller C,Alon T,Watson KL,Verrijzer CP,Goldman-Levi R,Zak NB

更新日期：1999-02-01 00:00:00

abstract：:Although RNA and RNA-binding proteins have been linked to double-strand breaks (DSBs), little is known regarding their roles in the cellular response to DSBs and, if any, in the repair process. Here, we provide direct evidence for the presence of RNA-DNA hybrids at DSBs and suggest that binding of RNA to DNA at DSBs m...

journal_title：Molecular and cellular biology

authors： Li L,Germain DR,Poon HY,Hildebrandt MR,Monckton EA,McDonald D,Hendzel MJ,Godbout R

更新日期：2016-10-28 00:00:00

abstract：:Eukaryotic translation initiation factor 4GI (eIF4GI) is an essential protein that is the target for translational regulation in many cellular processes and viral systems. It has been shown to function in both cap-dependent and cap-independent translation initiation by recruiting the 40S ribosomal subunit to the mRNA ...

journal_title：Molecular and cellular biology

authors： Byrd MP,Zamora M,Lloyd RE

更新日期：2002-07-01 00:00:00

abstract：:Human La protein has been shown to serve as a transcription factor for RNA polymerase III (pol III) by facilitating transcription termination and recycling of transcription complexes. In addition, La binds to the 3' oligo(U) ends common to all nascent pol III transcripts, and in the case of B1-Alu RNA, protects it fro...

journal_title：Molecular and cellular biology

authors： Goodier JL,Fan H,Maraia RJ

更新日期：1997-10-01 00:00:00

abstract：:Recombinant type 1 transforming growth factor beta (TGF-beta) was expressed to high levels in CHO cells by using dihydrofolate reductase (dhfr) gene amplification. The expression plasmid was derived from the pSV2 vectors and contained, in tandem, the simian TGF-beta and mouse dhfr cDNAs. Transcription of both cDNAs wa...

journal_title：Molecular and cellular biology

authors： Gentry LE,Webb NR,Lim GJ,Brunner AM,Ranchalis JE,Twardzik DR,Lioubin MN,Marquardt H,Purchio AF

更新日期：1987-10-01 00:00:00

abstract：:We have previously shown that the SIS/platelet-derived growth factor B chain contains a nuclear targeting signal near its C terminus. Here we show that the platelet-derived growth factor A chain also contains a nuclear targeting signal encoded by an exon which is subject to alternative splicing. This sequence is capab...

journal_title：Molecular and cellular biology

authors： Maher DW,Lee BA,Donoghue DJ

更新日期：1989-05-01 00:00:00

abstract：:Polyoma virus transforms, upon infection or DNA transfection, nonpermissive Fisher rat fibroblasts. Cloned viral DNA was deleted of sequences around the Bg/I site at nucleotide 86 by Bal31 nuclease treatment and then recloned in Escherichia coli. The extent of deletion for each mutant was then determined by DNA sequen...

journal_title：Molecular and cellular biology

authors： Katinka M,Yaniv M

更新日期：1982-10-01 00:00:00

abstract：:Rapid kinetic techniques were employed to measure the transport of adenine in adenine phosphoribosyltransferase-deficient L929 and Chinese hamster ovary (CHO) cells in zero-trans entry and exit and equilibrium exchange procedures. The kinetic parameters of transport were computed by fitting appropriate integrated rate...

journal_title：Molecular and cellular biology

authors： Puziss MB,Wohlhueter RM,Plagemann PG

更新日期：1983-01-01 00:00:00

abstract：:The retinoblastoma tumor suppressor protein (RB) is a potent inhibitor of cell proliferation. RB is expressed throughout the cell cycle, but its antiproliferative activity is neutralized by phosphorylation during the G(1)/S transition. RB plays an essential role in the G(1) arrest induced by a variety of growth inhibi...

journal_title：Molecular and cellular biology

authors： Knudsen KE,Booth D,Naderi S,Sever-Chroneos Z,Fribourg AF,Hunton IC,Feramisco JR,Wang JY,Knudsen ES

更新日期：2000-10-01 00:00:00

abstract：:Inactivating mutations within the von Hippel-Lindau (VHL) tumor suppressor gene predispose patients to develop a variety of highly vascularized tumors. pVHL targets alpha subunits of the heterodimeric transcription factor hypoxia-inducible factor (HIF), a critical regulator of energy metabolism, angiogenesis, hematopo...

journal_title：Molecular and cellular biology

authors： Mack FA,Patel JH,Biju MP,Haase VH,Simon MC

更新日期：2005-06-01 00:00:00

abstract：:Physiological cell deaths occur ubiquitously throughout biology and have common attributes, including apoptotic morphology with mitosis-like chromatin condensation and prelytic genome digestion. The fundamental question is whether a common mechanism of dying underlies these common hallmarks of death. Here we describe ...

journal_title：Molecular and cellular biology

authors： Harvey KJ,Blomquist JF,Ucker DS

更新日期：1998-05-01 00:00:00

abstract：:Genomic imprinting results in parent-specific monoallelic expression of a small number of genes in mammals. The identity of imprints is unknown, but much evidence points to a role for DNA methylation. The maternal alleles of the imprinted H19 gene are active and hypomethylated; the paternal alleles are inactive and hy...

journal_title：Molecular and cellular biology

authors： Szabó PE,Pfeifer GP,Mann JR

更新日期：1998-11-01 00:00:00

abstract：:G proteins are molecular switches that control a wide variety of physiological functions, including neurotransmission, transcriptional activation, cell migration, cell growth. and proliferation. The ability of GTPases to participate in signaling events is determined by the ratio of GTP-bound to GDP-bound forms in the ...

journal_title：Molecular and cellular biology

authors： Xia C,Ma W,Stafford LJ,Liu C,Gong L,Martin JF,Liu M

更新日期：2003-04-01 00:00:00

abstract：:The turnover of the colony-stimulating factor 1 receptor (CSF-1R), the c-fms proto-oncogene product, is accelerated by ligand binding or by activators of protein kinase C (PKC), such as the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). The mechanisms of ligand- and TPA-induced downmodulation were shown to ...

journal_title：Molecular and cellular biology

authors： Downing JR,Roussel MF,Sherr CJ

更新日期：1989-07-01 00:00:00

abstract：:F9 embryonic teratocarcinoma stem cells differentiate into an epithelial cell type called extraembryonic endoderm when treated with retinoic acid (RA), a derivative of retinol (vitamin A). This differentiation is presumably mediated through the actions of retinoid receptors, the RARs and RXRs. To delineate the functio...

journal_title：Molecular and cellular biology

authors： Boylan JF,Lufkin T,Achkar CC,Taneja R,Chambon P,Gudas LJ

更新日期：1995-02-01 00:00:00

abstract：:U6 RNA genes from the trypanosomatids Crithidia fasciculata and Leptomonas seymouri have been isolated and sequenced. As in Trypanosoma brucei, the U6 RNA genes in both C. fasciculata and L. seymouri are arranged in close linkage with upstream tRNA genes. The U6 RNA sequences from C. fasciculata and L. seymouri deviat...

journal_title：Molecular and cellular biology

authors： Xu GL,Wieland B,Bindereif A

更新日期：1994-07-01 00:00:00

abstract：:SEC63 encodes a protein required for secretory protein translocation into the endoplasmic reticulum (ER) of Saccharomyces cerevisiae (J. A. Rothblatt, R. J. Deshaies, S. L. Sanders, G. Daum, and R. Schekman, J. Cell Biol. 109:2641-2652, 1989). Antibody directed against a recombinant form of the protein detects a 73-kD...

journal_title：Molecular and cellular biology

authors： Feldheim D,Rothblatt J,Schekman R

更新日期：1992-07-01 00:00:00

abstract：:As shown by Southern blot analysis, the metallothionein-1 (MT-1) genes in rats comprise a multigene family. We present the sequence of the MT-1 structural gene and compare its features with other metallothionein genes. Three MT-1 pseudogenes which we sequenced apparently arose by reverse transcription of processed mRN...

journal_title：Molecular and cellular biology

authors： Andersen RD,Birren BW,Taplitz SJ,Herschman HR

更新日期：1986-01-01 00:00:00

abstract：:Biallelic expression of Igf2 is frequently seen in cancers because Igf2 functions as a survival factor. In many tumors the activation of Igf2 expression has been correlated with de novo methylation of the imprinted region. We have compared the intrinsic susceptibilities of the imprinted region of Igf2 and H19, other i...

journal_title：Molecular and cellular biology

authors： Biniszkiewicz D,Gribnau J,Ramsahoye B,Gaudet F,Eggan K,Humpherys D,Mastrangelo MA,Jun Z,Walter J,Jaenisch R

更新日期：2002-04-01 00:00:00

abstract：:We have isolated a full-length mouse cDNA encoding a lysine-rich protein of 1,131 amino acids with a calculated molecular mass of 126 kDa. The protein binds in a sequence-unspecific manner to DNA, is localized exclusively in the nucleus, and contains a putative ATP binding site and a stretch of 80 amino acids with hom...

journal_title：Molecular and cellular biology

authors： Luckow B,Bunz F,Stillman B,Lichter P,Schütz G

更新日期：1994-03-01 00:00:00

abstract：:DNA sequences encoding a human melanoma membrane-bound sialoglycoprotein of 130,000 molecular weight (gp130) were introduced into a clonal derivative of mouse B-16 melanoma cells with the selectable neomycin resistance gene (aminoglycoside phosphotransferase). Mouse transfectants were identified by a rapid and precise...

journal_title：Molecular and cellular biology

authors： Albino AP,Graf LH Jr,Kantor RR,McLean W,Silagi S,Old LJ

更新日期：1985-04-01 00:00:00

abstract：:A novel small nuclear ribonucleoprotein (snRNP) complex containing both U11 and U12 RNAs has been identified in HeLa cell extracts. This U11/U12 snRNP complex can be visualized on glycerol gradients, on native polyacrylamide gels, and by selection with antisense 2'-O-methyl oligoribonucleotides. RNase H-mediated degra...

journal_title：Molecular and cellular biology

authors： Wassarman KM,Steitz JA

更新日期：1992-03-01 00:00:00

abstract：:Phosphorylation is important in p53-mediated DNA damage responses. After UV irradiation, p53 is phosphorylated specifically at murine residue Ser389. Phosphorylation mutant p53.S389A cells and mice show reduced apoptosis and compromised tumor suppression after UV irradiation. We investigated the underlying cellular pr...

journal_title：Molecular and cellular biology

authors： Bruins W,Bruning O,Jonker MJ,Zwart E,van der Hoeven TV,Pennings JL,Rauwerda H,de Vries A,Breit TM

更新日期：2008-03-01 00:00:00

abstract：:alpha 1-Antitrypsin (AT), the major elastase inhibitor in mammalian serum, is produced primarily in the liver. We have characterized AT gene structure and expression in the mouse species Mus caroli, which expresses high levels of AT in the kidneys as well as in the liver. Analysis of cDNA and genomic clones showed tha...

journal_title：Molecular and cellular biology

authors： Latimer JJ,Berger FG,Baumann H

更新日期：1990-02-01 00:00:00

abstract：:We set up an alternative splicing system in vitro in which the relative amounts of two spliced RNAs, one containing and the other lacking a particular exon, were directly proportional to the length of an inverted repeat inserted into the flanking introns. We then used the system to measure the effect of intramolecular...

journal_title：Molecular and cellular biology

authors： Solnick D,Lee SI

更新日期：1987-09-01 00:00:00