A carboxy-terminal basic region controls RNA polymerase III transcription factor activity of human La protein.

abstract：:Approximately half of the introns in Drosophila melanogaster are too small to function in a vertebrate and often lack the pyrimidine tract associated with vertebrate 3' splice sites. Here, we report the splicing and spliceosome assembly properties of two such introns: one with a pyrimidine-poor 3' splice site and one ...

journal_title：Molecular and cellular biology

authors： Talerico M,Berget SM

更新日期：1994-05-01 00:00:00

abstract：:Gene expression programs required for differentiation depend on both DNA-bound transcription factors and surrounding histone modifications. Expression of the basic helix-loop-helix (bHLH) protein NeuroD1 is restricted to endocrine cells in the gastrointestinal (GI) tract, where it is important for endocrine differenti...

journal_title：Molecular and cellular biology

authors： Ray SK,Li HJ,Metzger E,Schüle R,Leiter AB

更新日期：2014-06-01 00:00:00

abstract：:Sequence analysis of a mouse testicular alpha-tubulin partial cDNA, pRD alpha TT1, reveals an isotype that differs from both the somatic and the predominant testicular alpha tubulins at approximately 30% of the 212 amino acid residues determined. Although this mouse testicular cDNA retains the highly conserved sequenc...

journal_title：Molecular and cellular biology

authors： Hecht NB,Distel RJ,Yelick PC,Tanhauser SM,Driscoll CE,Goldberg E,Tung KS

更新日期：1988-02-01 00:00:00

abstract：:We demonstrated that MIF-1, identified initially as a binding activity that associated with the intron I element of the c-myc gene, consists of two polypeptides, the myc intron-binding peptide (MIBP1) and the major histocompatibility class II promoter-binding protein, RFX1. Using a polyclonal antiserum directed agains...

journal_title：Molecular and cellular biology

authors： Reinhold W,Emens L,Itkes A,Blake M,Ichinose I,Zajac-Kaye M

更新日期：1995-06-01 00:00:00

abstract：:TGF-alpha-PE40 is a hybrid protein composed of transforming growth factor-alpha (TGF-alpha) fused to a 40,000-dalton segment of Pseudomonas exotoxin A (PE40). This hybrid protein possesses the receptor-binding activity of TGF-alpha and the cell-killing properties of PE40. These properties enable TGF-alpha-PE40 to bind...

journal_title：Molecular and cellular biology

authors： Edwards GM,DeFeo-Jones D,Tai JY,Vuocolo GA,Patrick DR,Heimbrook DC,Oliff A

更新日期：1989-07-01 00:00:00

abstract：:The carcinoembryonic antigen (CEA) family consists of a large group of evolutionarily and structurally divergent glycoproteins. The murine CEACAM9 and CEACAM11-related proteins as well as the pregnancy-specific glycoproteins (PSG) are secreted members of the CEA family which are differentially expressed in fetal troph...

journal_title：Molecular and cellular biology

authors： Finkenzeller D,Fischer B,Lutz S,Schrewe H,Shimizu T,Zimmermann W

更新日期：2003-01-01 00:00:00

abstract：:Insulin stimulates glucose transport into fat and muscle cells by increasing the exocytic trafficking rate of the GLUT4 facilitative glucose transporter from intracellular stores to the plasma membrane. Delivery of GLUT4 to the plasma membrane is mediated by formation of functional SNARE complexes containing syntaxin4...

journal_title：Molecular and cellular biology

authors： Kioumourtzoglou D,Gould GW,Bryant NJ

更新日期：2014-04-01 00:00:00

abstract：:We have identified a new immunoglobulin heavy-chain enhancer element, designated pi, between the microE2 and microE3 elements. The pi enhancer element is transcriptionally active primarily during early stages of B-cell development but becomes virtually inactive during B-cell maturation at about the stage of immunoglob...

journal_title：Molecular and cellular biology

authors： Libermann TA,Baltimore D

更新日期：1993-10-01 00:00:00

abstract：:We have recently reported that the yeast chromatin-remodeling factor Swi1 can exist as a prion, [SWI(+)], demonstrating a link between prionogenesis and global transcriptional regulation. To shed light on how the Swi1 conformational switch influences Swi1 function and to define the sequence and structural requirements...

journal_title：Molecular and cellular biology

authors： Du Z,Crow ET,Kang HS,Li L

更新日期：2010-10-01 00:00:00

abstract：:The Saccharomyces cerevisiae Trf4 and Trf5 proteins are members of a distinct family of eukaryotic DNA polymerase beta-like nucleotidyltransferases, and a template-dependent DNA polymerase activity has been reported for Trf4. To define the nucleotidyltransferase activities associated with Trf4 and Tr5, we purified the...

journal_title：Molecular and cellular biology

authors： Haracska L,Johnson RE,Prakash L,Prakash S

更新日期：2005-11-01 00:00:00

abstract：:Alternative splicing of the locus AbetaH-J-J generates three functionally distinct proteins: an enzyme, AbetaH (aspartyl-beta-hydroxylase), a structural protein of the sarcoplasmic reticulum membrane (junctin), and an integral membrane calcium binding protein (junctate). Junctin and junctate are two important proteins...

journal_title：Molecular and cellular biology

authors： Feriotto G,Finotti A,Volpe P,Treves S,Ferrari S,Angelelli C,Zorzato F,Gambari R

更新日期：2005-04-01 00:00:00

abstract：:Insulin treatment of Chinese hamster ovary (CHO) cells expressing high levels of the insulin receptor (CHO/IR cells) activates both c-fos serum response element and activator protein 1 (AP-1) reporter genes approximately 10-fold. In contrast, parental CHO cells display only two- to threefold insulin stimulation of rep...

journal_title：Molecular and cellular biology

authors： Yamauchi K,Pessin JE

更新日期：1994-07-01 00:00:00

abstract：:Human insulin-like growth factor II (IGF-II) mRNAs are subject to site-specific endonucleolytic cleavage in the 3' untranslated region, leading to an unstable 5' cleavage product containing the IGF-II coding region and a very stable 3' cleavage product of 1.8 kb. This endonucleolytic cleavage is most probably the firs...

journal_title：Molecular and cellular biology

authors： Scheper W,Meinsma D,Holthuizen PE,Sussenbach JS

更新日期：1995-01-01 00:00:00

abstract：:Nuclear extracts from chicken erythroid cells selectively stimulate transcription of the chicken histone H5 gene (and not of other chicken histone genes) after coinjection into frog oocytes. This effect is shown to involve an enhancerlike activity, and a region of the H5 gene sufficient to mediate trans-activation is ...

journal_title：Molecular and cellular biology

authors： Wigley PL,Wells JR

更新日期：1987-10-01 00:00:00

abstract：:We have analyzed the 5'-flanking region of one of the genes coding for the human acute-phase protein, serum amyloid A (SAA). We found that SAA mRNA could be increased fivefold in transfected cells by treatment with phorbol 12-myristate 13-acetate (PMA). To analyze this observation further, we placed a 265-base-pair 5'...

journal_title：Molecular and cellular biology

authors： Edbrooke MR,Burt DW,Cheshire JK,Woo P

更新日期：1989-05-01 00:00:00

abstract：:Plasmid DNA substrates were X-irradiated and injected into the nuclei of Xenopus laevis oocytes. After incubation for 20 h, DNA was recovered from the oocytes and analyzed simultaneously for repair and for intermolecular homologous recombination by electrophoresis and bacterial transformation. Oocyte-mediated repair o...

journal_title：Molecular and cellular biology

authors： Sweigert SE,Carroll D

更新日期：1990-11-01 00:00:00

abstract：:The RNA-binding protein (RBP) nucleolin promotes the expression of several proliferative proteins. Nucleolin levels are high in cancer cells, but the mechanisms that control nucleolin expression are unknown. Here, we show that nucleolin abundance is controlled posttranscriptionally via factors that associate with its ...

journal_title：Molecular and cellular biology

authors： Tominaga K,Srikantan S,Lee EK,Subaran SS,Martindale JL,Abdelmohsen K,Gorospe M

更新日期：2011-10-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the MET25 gene encodes O-acetylhomoserine sulfhydrylase. Synthesis of this enzyme is repressed by the presence of S-adenosylmethionine (AdoMet) in the growth medium. We identified cis elements required for MET25 expression by analyzing small deletions in the MET25 promoter region. The resu...

journal_title：Molecular and cellular biology

authors： Thomas D,Cherest H,Surdin-Kerjan Y

更新日期：1989-08-01 00:00:00

abstract：:The homeo box gene even-skipped (eve) encodes a 376-amino-acid protein that binds with high affinity to sequences located near the 5' termini of the eve and en genes. The 5' en sites are A + T rich and contain copies of the 10-base-pair (bp) consensus sequence T-C-A-A-T-T-A-A-A-T. In contrast, the 5' eve sites are G +...

journal_title：Molecular and cellular biology

authors： Hoey T,Warrior R,Manak J,Levine M

更新日期：1988-11-01 00:00:00

abstract：:Though widely recognized in higher eukaryotes, the regulation of Saccharomyces cerevisiae genes transcribed by RNA polymerase II by proteins that bind within the coding sequence remains largely speculative. We have shown for the LPD1 gene, encoding lipoamide dehydrogenase, that the coding sequence between +13 and +469...

journal_title：Molecular and cellular biology

authors： Sinclair DA,Kornfeld GD,Dawes IW

更新日期：1994-01-01 00:00:00

abstract：:Mrc1 is a conserved checkpoint mediator protein that transduces the replication stress signal to the downstream effector kinase. The loss of mrc1 checkpoint activity results in the aberrant activation of late/dormant origins in the presence of hydroxyurea. Mrc1 was also suggested to regulate orders of early origin fir...

journal_title：Molecular and cellular biology

authors： Matsumoto S,Kanoh Y,Shimmoto M,Hayano M,Ueda K,Fukatsu R,Kakusho N,Masai H

更新日期：2017-03-17 00:00:00

abstract：:The interaction of the retinoblastoma susceptibility gene product (Rb)-related p107 protein with the E2F transcription factor in S-phase cells facilitates the formation of a multicomponent complex also containing cyclin A and the p33cdk2 kinase. We have created a series of p107 mutants to assess the ability of p107 to...

journal_title：Molecular and cellular biology

authors： Smith EJ,Nevins JR

更新日期：1995-01-01 00:00:00

abstract：:The Ets family transcription factor PU.1 is crucial for the regulation of hematopoietic development. Pu.1 is activated in hematopoietic stem cells and is expressed in mast cells, B cells, granulocytes, and macrophages but is switched off in T cells. Many of the transcription factors regulating Pu.1 have been identifie...

journal_title：Molecular and cellular biology

authors： Hoogenkamp M,Krysinska H,Ingram R,Huang G,Barlow R,Clarke D,Ebralidze A,Zhang P,Tagoh H,Cockerill PN,Tenen DG,Bonifer C

更新日期：2007-11-01 00:00:00

abstract：:SCF complexes are E3 ubiquitin-protein ligases that mediate degradation of regulatory and signaling proteins and control G1/S cell cycle progression by degradation of G1 cyclins and the cyclin-dependent kinase inhibitor, Sic1. Interchangeable F-box proteins bind the core SCF components; each recruits a specific subset...

journal_title：Molecular and cellular biology

authors： Ivantsiv Y,Kaplun L,Tzirkin-Goldin R,Shabek N,Raveh D

更新日期：2006-03-01 00:00:00

abstract：:RNase H1 in mammalian cells is present in nuclei and mitochondria. Its absence in mitochondria results in embryonic lethality due to the failure to amplify mitochondrial DNA (mtDNA). Dual localization to mitochondria and nuclei results from differential translation initiation at two in-frame AUGs (M1 and M27) of a sin...

journal_title：Molecular and cellular biology

authors： Suzuki Y,Holmes JB,Cerritelli SM,Sakhuja K,Minczuk M,Holt IJ,Crouch RJ

更新日期：2010-11-01 00:00:00

abstract：:Human myeloid leukemia cells respond to 12-O-tetradecanoylphorbol-13-acetate (TPA) and other activators of protein kinase C (PKC) with induction of monocytic differentiation. The present studies demonstrated that treatment of U-937 and HL-60 myeloid leukemia cells with TPA, phorbol-12,13-dibutyrate, or bryostatin 1 wa...

journal_title：Molecular and cellular biology

authors： Kaneki M,Kharbanda S,Pandey P,Yoshida K,Takekawa M,Liou JR,Stone R,Kufe D

更新日期：1999-01-01 00:00:00

abstract：:The tumor suppressor p21 acts as a cell cycle inhibitor and has also been shown to regulate gene expression by functioning as a transcription corepressor. Here, we identified p21-regulated microRNAs (miRNAs) by sequencing small RNAs from isogenic p21(+/+) and p21(-/-) cells. Three abundant miRNA clusters, miR-200b-200...

journal_title：Molecular and cellular biology

authors： Li XL,Hara T,Choi Y,Subramanian M,Francis P,Bilke S,Walker RL,Pineda M,Zhu Y,Yang Y,Luo J,Wakefield LM,Brabletz T,Park BH,Sharma S,Chowdhury D,Meltzer PS,Lal A

更新日期：2014-02-01 00:00:00

abstract：:Smads 1, 5, and 8 are the intracellular mediators for the bone morphogenetic proteins (BMPs), which play crucial roles during mammalian development. Previous research has shown that Smad1 is important in the formation of the allantois, while Smad5 has been shown to be critical in the process of angiogenesis. To furthe...

journal_title：Molecular and cellular biology

authors： Hester M,Thompson JC,Mills J,Liu Y,El-Hodiri HM,Weinstein M

更新日期：2005-06-01 00:00:00

abstract：:The chromatin structure of TDH3, one of three genes encoding glyceraldehyde phosphate dehydrogenases in Saccharomyces cerevisiae, was analyzed by nuclease digestion. A large hypersensitive region was found at the TDH3 promoter extending from the RNA initiation site at position -40 to position -560. This hypersensitive...

journal_title：Molecular and cellular biology

authors： Pavlović B,Hörz W

更新日期：1988-12-01 00:00:00

abstract：:Both cDNA clones and a genomic DNA clone encoding a 509-amino-acid protein that is 64% similar to chicken pp60c-src were isolated from the simple metazoan Hydra attenuata. We have designated this gene STK, for src-type kinase. Features of the amino acid sequence of the protein encoded by the STK gene suggest that it i...

journal_title：Molecular and cellular biology

authors： Bosch TC,Unger TF,Fisher DA,Steele RE

更新日期：1989-10-01 00:00:00