Targeted disruption of retinoic acid receptor alpha (RAR alpha) and RAR gamma results in receptor-specific alterations in retinoic acid-mediated differentiation and retinoic acid metabolism.

abstract：:Murine embryonic stem (ES) cells are defined by continuous self-renewal and pluripotency. A diverse repertoire of protein isoforms arising from alternative splicing is expressed in ES cells without defined biological roles. Sall4, a transcription factor essential for pluripotency, exists as two isoforms (Sall4a and Sa...

journal_title：Molecular and cellular biology

authors： Rao S,Zhen S,Roumiantsev S,McDonald LT,Yuan GC,Orkin SH

更新日期：2010-11-01 00:00:00

abstract：:We investigated the interaction of phospholipase C-gamma (PLC-gamma) with wild-type and mutant forms of the platelet-derived growth factor (PDGF) beta-receptor both in vivo and in vitro. After PDGF treatment of CHO cell lines expressing wild-type or either of two mutant (delta Ki and Y825F) PDGF receptors, PLC-gamma b...

journal_title：Molecular and cellular biology

authors： Morrison DK,Kaplan DR,Rhee SG,Williams LT

更新日期：1990-05-01 00:00:00

abstract：:Tax1, a potent activator of human T-cell lymphotropic virus type 1 (HTLV-1) transcription, has been shown to modulate expression of many cellular genes. Tax1 does not bind DNA directly but regulates transcription through protein-protein interactions with sequence-specific transcription factors. Using the yeast two-hyb...

journal_title：Molecular and cellular biology

authors： Pise-Masison CA,Dittmer J,Clemens KE,Brady JN

更新日期：1997-03-01 00:00:00

abstract：:Formin homology (FH) proteins are implicated in cell polarization and cytokinesis through actin organization. There are two FH proteins in the yeast Saccharomyces cerevisiae, Bni1p and Bnr1p. Bni1p physically interacts with Rho family small G proteins (Rho1p and Cdc42p), actin, two actin-binding proteins (profilin and...

journal_title：Molecular and cellular biology

authors： Ozaki-Kuroda K,Yamamoto Y,Nohara H,Kinoshita M,Fujiwara T,Irie K,Takai Y

更新日期：2001-02-01 00:00:00

abstract：:Prdm1/Blimp-1 is a master regulator of gene expression in diverse tissues of the developing embryo and adult organism. Its C-terminal zinc finger domain mediates nuclear import, DNA binding, and recruitment of the corepressors G9a and HDAC1/2. Alternatively spliced transcripts lacking exon 7 sequences encode a structu...

journal_title：Molecular and cellular biology

authors： Morgan MA,Mould AW,Li L,Robertson EJ,Bikoff EK

更新日期：2012-09-01 00:00:00

abstract：:Resistance to the growth-inhibitory action of retinoic acid (RA), the bioactive derivative of vitamin A, is common in human tumors. One form of RA resistance has been associated with silencing and hypermethylation of the retinoic acid receptor beta2 gene (RARbeta2), an RA-regulated tumor suppressor gene. The presence ...

journal_title：Molecular and cellular biology

authors： Ren M,Pozzi S,Bistulfi G,Somenzi G,Rossetti S,Sacchi N

更新日期：2005-12-01 00:00:00

abstract：:Liver regeneration is a rapid and concerted response to injury, in which growth factor-generated intracellular signals result in activation of transcription factors, DNA synthesis, and hepatocyte proliferation. However, the link between cytoplasmic signals resulting in proliferative response to liver injury remains to...

journal_title：Molecular and cellular biology

authors： Bard-Chapeau EA,Yuan J,Droin N,Long S,Zhang EE,Nguyen TV,Feng GS

更新日期：2006-06-01 00:00:00

abstract：:With v-yes DNA as the probe, a human cDNA library made from placental RNA was screened under relaxed conditions, and DNA clones derived from a novel genetic locus, termed lyn, were obtained. Nucleotide sequencing revealed that lyn could encode a novel tyrosine kinase that was very similar to mouse T-lymphocyte-specifi...

journal_title：Molecular and cellular biology

authors： Yamanashi Y,Fukushige S,Semba K,Sukegawa J,Miyajima N,Matsubara K,Yamamoto T,Toyoshima K

更新日期：1987-01-01 00:00:00

abstract：:By using recombinase-mediated cassette exchange, a method that allows integration of single copies of different constructs at the same predetermined chromosomal location, several expression cassettes have been integrated at a randomly chosen locus in the genome of mouse erythroleukemia cells. The cassettes studied con...

journal_title：Molecular and cellular biology

authors： Feng YQ,Alami R,Bouhassira EE

更新日期：1999-07-01 00:00:00

abstract：:Lactoperoxidase was used to selectively radiolabel endocytic membrane. CHO cells were incubated with enzyme at 37 degrees C for 10 min to permit lactoperoxidase internalization. Radioiodination was done at 4 degrees C. About 90% of the radioiodinated products pelleted at 100,000 X g. From 12 to 15 different electropho...

journal_title：Molecular and cellular biology

authors： Storrie B,Dreesen TD,Maurey KM

更新日期：1981-03-01 00:00:00

abstract：:Rats and mice have two, equally expressed, nonallelic genes encoding preproinsulin (genes I and II). Cytological hybridization with metaphase chromosomes indicated that both genes reside on rat chromosome I but are approximately 100,000 kilobases apart. In mice the two genes reside on two different chromosomes. DNA se...

journal_title：Molecular and cellular biology

authors： Soares MB,Schon E,Henderson A,Karathanasis SK,Cate R,Zeitlin S,Chirgwin J,Efstratiadis A

更新日期：1985-08-01 00:00:00

abstract：:The basic leucine zipper transcription factor, CCAAT/enhancer binding protein alpha (C/EBPalpha), is abundantly expressed in keratinocytes of the skin; however, its function in skin is poorly characterized. UVB radiation is responsible for the majority of human skin cancers. In response to UVB-induced DNA damage, kera...

journal_title：Molecular and cellular biology

authors： Yoon K,Smart RC

更新日期：2004-12-01 00:00:00

abstract：:MIA/CD-RAP is a small, soluble protein secreted from malignant melanoma cells and from chondrocytes. Recent evidence has identified MIA/CD-RAP as the prototype of a small family of extracellular proteins adopting an SH3 domain-like fold. It is thought that interaction between MIA/CD-RAP and specific epitopes in extrac...

journal_title：Molecular and cellular biology

authors： Moser M,Bosserhoff AK,Hunziker EB,Sandell L,Fässler R,Buettner R

更新日期：2002-03-01 00:00:00

abstract：:Nucleotide excision repair and the long-patch mismatch repair systems correct abnormal DNA structures arising from DNA damage and replication errors, respectively. DNA synthesis past a damaged base (translesion replication) often causes misincorporation at the lesion site. In addition, mismatches are hot spots for DNA...

journal_title：Molecular and cellular biology

authors： Mu D,Tursun M,Duckett DR,Drummond JT,Modrich P,Sancar A

更新日期：1997-02-01 00:00:00

abstract：:To examine the role of clathrin-dependent insulin receptor internalization in insulin-stimulated signal transduction events, we expressed a dominant-interfering mutant of dynamin (K44A/dynamin) by using a recombinant adenovirus in the H4IIE hepatoma and 3T3L1 adipocyte cell lines. Expression of K44A/dynamin inhibited ...

journal_title：Molecular and cellular biology

authors： Ceresa BP,Kao AW,Santeler SR,Pessin JE

更新日期：1998-07-01 00:00:00

abstract：:The chicken immunoglobulin light-chain gene (IgL) encodes only a single variable gene segment capable of recombination. To generate an immune repertoire, chickens diversify this unique rearranged VL gene segment during B-cell development in the bursa of Fabricius. Sequence analysis of IgL cDNAs suggests that both gene...

journal_title：Molecular and cellular biology

authors： Kim S,Humphries EH,Tjoelker L,Carlson L,Thompson CB

更新日期：1990-06-01 00:00:00

abstract：:DNA from the centromere region of linkage group (LG) VII of Neurospora crassa was cloned previously from a yeast artificial chromosome library and was found to be atypical of Neurospora DNA in both composition (AT rich) and complexity (repetitive). We have determined the DNA sequence of a small portion (approximately ...

journal_title：Molecular and cellular biology

authors： Cambareri EB,Aisner R,Carbon J

更新日期：1998-09-01 00:00:00

abstract：:Bone-specific transcription of the osteocalcin (OC) gene is regulated principally by the Runx2 transcription factor and is further stimulated in response to 1alpha,25-dihydroxyvitamin D3 via its specific receptor (VDR). The rat OC gene promoter contains three recognition sites for Runx2 (sites A, B, and C). Mutation o...

journal_title：Molecular and cellular biology

authors： Paredes R,Arriagada G,Cruzat F,Villagra A,Olate J,Zaidi K,van Wijnen A,Lian JB,Stein GS,Stein JL,Montecino M

更新日期：2004-10-01 00:00:00

abstract：:A significant challenge to our understanding of eukaryotic transcriptional regulation is to determine how multiple signal transduction pathways converge on a single promoter to regulate transcription in divergent fashions. To study this, we have investigated the transcriptional regulation of the Schizosaccharomyces po...

journal_title：Molecular and cellular biology

authors： Neely LA,Hoffman CS

更新日期：2000-09-01 00:00:00

abstract：:Considering the link between plasma high-density lipoprotein (HDL) cholesterol levels and a protective effect against coronary artery disease as well as the suggested beneficial effects of retinoids on the production of the major HDL apolipoprotein (apo), apo A-I, the goal of this study was to analyze the influence of...

journal_title：Molecular and cellular biology

authors： Vu-Dac N,Schoonjans K,Kosykh V,Dallongeville J,Heyman RA,Staels B,Auwerx J

更新日期：1996-07-01 00:00:00

abstract：:The influence of transcription on homologous intrachromosomal recombination between direct and inverted repeats has been examined by using Chinese hamster ovary cells. Recombination was monitored between two integrated neomycin (neo) genes, including one silent allele and a second allele regulated by the inducible mou...

journal_title：Molecular and cellular biology

authors： Nickoloff JA

更新日期：1992-12-01 00:00:00

abstract：:We have been interested in how Rous sarcoma virus (RSV) influences transformed cell morphology and compared the molecular properties of chicken embryo cells (CEC) infected with mutants of RSV that induce the fusiform transformed cell morphology with those of CEC infected by wild-type RSV, which induces the more normal...

journal_title：Molecular and cellular biology

authors： Rohrschneider L,Reynolds S

更新日期：1985-11-01 00:00:00

abstract：:Cytosine methylation at CpG dinucleotides is thought to cause more than one-third of all transition mutations responsible for human genetic diseases and cancer. We investigated the methylation status of the CpG dinucleotide at codon 248 in exon 7 of the p53 gene because this codon is a hot spot for inactivating mutati...

journal_title：Molecular and cellular biology

authors： Magewu AN,Jones PA

更新日期：1994-06-01 00:00:00

abstract：:Up to 70% of human genes are associated with regions of nonmethylated DNA called CpG islands (S. Saxonov, P. Berg, and D. L. Brutlag, Proc. Natl. Acad. Sci. U. S. A. 103:1412-1417, 2006). Usually associated with the 5' end of genes, CpG islands are thought to impact gene expression. We previously demonstrated that the...

journal_title：Molecular and cellular biology

authors： Zhou JC,Blackledge NP,Farcas AM,Klose RJ

更新日期：2012-01-01 00:00:00

abstract：:We have analyzed the fine structure of DNA repair in Chinese hamster ovary (CHO) cells within the G1 and G2 phases of the cell cycle. Repair of inactive regions of the genome has been suggested to increase in the G2 phase of the cell cycle compared with other phases. However, detailed studies of DNA repair in the G2 p...

journal_title：Molecular and cellular biology

authors： Petersen LN,Orren DK,Bohr VA

更新日期：1995-07-01 00:00:00

abstract：:R2 non-long terminal repeat retrotransposable elements insert at a unique site in the 28S rRNA genes of insects. The protein encoded by the single open reading frame of R2 is capable of conducting the initial steps of its integration in vitro. The protein nicks the noncoding strand of the 28S target DNA (the strand wh...

journal_title：Molecular and cellular biology

authors： Luan DD,Eickbush TH

更新日期：1996-09-01 00:00:00

abstract：:The ETS transcription factor complex GABP consists of the GABPalpha protein, containing an ETS DNA binding domain, and an unrelated GABPbeta protein, containing a transactivation domain and nuclear localization signal. GABP has been shown in vitro to regulate the expression of nuclear genes involved in mitochondrial r...

journal_title：Molecular and cellular biology

authors： Ristevski S,O'Leary DA,Thornell AP,Owen MJ,Kola I,Hertzog PJ

更新日期：2004-07-01 00:00:00

abstract：:Signal transducers and activators of transcription (STATs) are transcription factors that mediate normal biologic responses to cytokines and growth factors. However, abnormal activation of certain STAT family members, including Stat3, is increasingly associated with oncogenesis. In fibroblasts expressing the Src oncop...

journal_title：Molecular and cellular biology

authors： Turkson J,Bowman T,Adnane J,Zhang Y,Djeu JY,Sekharam M,Frank DA,Holzman LB,Wu J,Sebti S,Jove R

更新日期：1999-11-01 00:00:00

abstract：:To analyze determinants within the Na,K-ATPase alpha subunit that contribute to differential ouabain sensitivity, we constructed and expressed a panel of chimeric cDNA molecules between ouabain-resistant and ouabain-sensitive alpha subunit cDNAs. When introduced into ouabain-sensitive monkey CV-1 cells, ouabain-resist...

journal_title：Molecular and cellular biology

authors： Emanuel JR,Graw S,Housman D,Levenson R

更新日期：1989-09-01 00:00:00

abstract：:The Cyp cell line consists of mouse cells transformed by a thermosensitive polyomavirus (Py) genome and routinely propagated at 39 degrees C. Cyp cells are readily induced to synthesize free Py DNA by being transferred to 33 degrees C. In one subclone (C12/a1/S48, or S48) of this line, such induction resulted in the i...

journal_title：Molecular and cellular biology

authors： Huberdeau D,Sylla BS,Herring-Gillam E,Bourgaux-Ramoisy D,Bourgaux P

更新日期：1985-10-01 00:00:00