Abstract:
:The ETS transcription factor complex GABP consists of the GABPalpha protein, containing an ETS DNA binding domain, and an unrelated GABPbeta protein, containing a transactivation domain and nuclear localization signal. GABP has been shown in vitro to regulate the expression of nuclear genes involved in mitochondrial respiration and neuromuscular signaling. We investigated the in vivo function of GABP by generating a null mutation in the murine Gabpalpha gene. Embryos homozygous for the null Gabpalpha allele die prior to implantation, consistent with the broad expression of Gabpalpha throughout embryogenesis and in embryonic stem cells. Gabpalpha(+/-) mice demonstrated no detectable phenotype and unaltered protein levels in the panel of tissues examined. This indicates that Gabpalpha protein levels are tightly regulated to protect cells from the effects of loss of Gabp complex function. These results show that Gabpalpha function is essential and is not compensated for by other ETS transcription factors in the mouse, and they are consistent with a specific requirement for Gabp expression for the maintenance of target genes involved in essential mitochondrial cellular functions during early cleavage events of the embryo.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Ristevski S,O'Leary DA,Thornell AP,Owen MJ,Kola I,Hertzog PJdoi
10.1128/MCB.24.13.5844-5849.2004subject
Has Abstractpub_date
2004-07-01 00:00:00pages
5844-9issue
13eissn
0270-7306issn
1098-5549pii
24/13/5844journal_volume
24pub_type
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